Experiment: D-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5861 and Pf1N1B4_5862 are separated by 52 nucleotides Pf1N1B4_5862 and Pf1N1B4_5863 overlap by 1 nucleotides
Pf1N1B4_5861 - Alpha/beta hydrolase fold (EC 3.8.1.5), at 295,362 to 296,288
_5861
Pf1N1B4_5862 - Glycolate dehydrogenase (EC 1.1.99.14), subunit GlcD, at 296,341 to 297,840
_5862
Pf1N1B4_5863 - Glycolate dehydrogenase (EC 1.1.99.14), FAD-binding subunit GlcE, at 297,840 to 298,889
_5863
Position (kb) 296
297
298 Strain fitness (log2 ratio) -2
-1
0
1 at 295.440 kb on + strand at 295.441 kb on - strand at 295.575 kb on + strand, within Pf1N1B4_5861 at 295.641 kb on - strand, within Pf1N1B4_5861 at 295.756 kb on + strand, within Pf1N1B4_5861 at 295.757 kb on - strand, within Pf1N1B4_5861 at 295.826 kb on + strand, within Pf1N1B4_5861 at 295.944 kb on + strand, within Pf1N1B4_5861 at 295.950 kb on + strand at 296.768 kb on - strand, within Pf1N1B4_5862 at 296.854 kb on + strand, within Pf1N1B4_5862 at 296.867 kb on + strand, within Pf1N1B4_5862 at 296.868 kb on - strand, within Pf1N1B4_5862 at 297.395 kb on - strand, within Pf1N1B4_5862 at 297.513 kb on + strand, within Pf1N1B4_5862 at 297.514 kb on - strand, within Pf1N1B4_5862 at 298.003 kb on - strand, within Pf1N1B4_5863 at 298.003 kb on - strand, within Pf1N1B4_5863 at 298.306 kb on - strand, within Pf1N1B4_5863 at 298.554 kb on + strand at 298.583 kb on - strand, within Pf1N1B4_5863 at 298.583 kb on - strand, within Pf1N1B4_5863
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lactate (C) remove 295,440 + -0.8 295,441 - -0.6 295,575 + Pf1N1B4_5861 0.23 -0.2 295,641 - Pf1N1B4_5861 0.30 -1.4 295,756 + Pf1N1B4_5861 0.43 +0.1 295,757 - Pf1N1B4_5861 0.43 -0.5 295,826 + Pf1N1B4_5861 0.50 +0.1 295,944 + Pf1N1B4_5861 0.63 +1.4 295,950 + +1.5 296,768 - Pf1N1B4_5862 0.28 -1.0 296,854 + Pf1N1B4_5862 0.34 -1.1 296,867 + Pf1N1B4_5862 0.35 -0.8 296,868 - Pf1N1B4_5862 0.35 -1.8 297,395 - Pf1N1B4_5862 0.70 -2.2 297,513 + Pf1N1B4_5862 0.78 -1.1 297,514 - Pf1N1B4_5862 0.78 +0.0 298,003 - Pf1N1B4_5863 0.16 -1.2 298,003 - Pf1N1B4_5863 0.16 -1.1 298,306 - Pf1N1B4_5863 0.44 -1.6 298,554 + -2.2 298,583 - Pf1N1B4_5863 0.71 +0.2 298,583 - Pf1N1B4_5863 0.71 -1.0
Or see this region's nucleotide sequence