Experiment: D-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5706 and Pf1N1B4_5707 are separated by 10 nucleotides Pf1N1B4_5707 and Pf1N1B4_5708 are separated by 84 nucleotides
Pf1N1B4_5706 - Fumarylacetoacetate hydrolase family protein, at 144,539 to 145,660
_5706
Pf1N1B4_5707 - NADH dehydrogenase (EC 1.6.99.3), at 145,671 to 146,762
_5707
Pf1N1B4_5708 - Transcriptional regulator, TetR family, at 146,847 to 147,461
_5708
Position (kb) 145
146
147 Strain fitness (log2 ratio) -2
-1
0
1
2 at 144.884 kb on + strand, within Pf1N1B4_5706 at 144.885 kb on - strand, within Pf1N1B4_5706 at 144.910 kb on + strand, within Pf1N1B4_5706 at 144.978 kb on + strand, within Pf1N1B4_5706 at 145.436 kb on + strand, within Pf1N1B4_5706 at 145.479 kb on - strand, within Pf1N1B4_5706 at 145.479 kb on - strand, within Pf1N1B4_5706 at 145.484 kb on + strand, within Pf1N1B4_5706 at 145.502 kb on + strand, within Pf1N1B4_5706 at 145.639 kb on - strand at 145.847 kb on + strand, within Pf1N1B4_5707 at 146.047 kb on + strand, within Pf1N1B4_5707 at 146.048 kb on - strand, within Pf1N1B4_5707 at 146.048 kb on - strand, within Pf1N1B4_5707 at 146.048 kb on - strand at 146.082 kb on - strand, within Pf1N1B4_5707 at 146.132 kb on + strand, within Pf1N1B4_5707 at 146.132 kb on + strand, within Pf1N1B4_5707 at 146.132 kb on + strand, within Pf1N1B4_5707 at 146.133 kb on - strand, within Pf1N1B4_5707 at 146.133 kb on - strand, within Pf1N1B4_5707 at 146.151 kb on + strand, within Pf1N1B4_5707 at 146.152 kb on - strand, within Pf1N1B4_5707 at 146.190 kb on - strand, within Pf1N1B4_5707 at 146.348 kb on + strand, within Pf1N1B4_5707 at 146.348 kb on + strand, within Pf1N1B4_5707 at 146.349 kb on - strand at 146.549 kb on - strand at 146.555 kb on - strand, within Pf1N1B4_5707 at 146.555 kb on - strand, within Pf1N1B4_5707 at 146.582 kb on - strand, within Pf1N1B4_5707 at 146.790 kb on - strand at 147.330 kb on - strand, within Pf1N1B4_5708 at 147.332 kb on - strand, within Pf1N1B4_5708 at 147.332 kb on - strand, within Pf1N1B4_5708 at 147.480 kb on - strand at 147.567 kb on + strand at 147.567 kb on + strand at 147.567 kb on + strand at 147.590 kb on + strand at 147.591 kb on - strand at 147.591 kb on - strand at 147.591 kb on - strand at 147.623 kb on + strand at 147.747 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lactate (C) remove 144,884 + Pf1N1B4_5706 0.31 -0.7 144,885 - Pf1N1B4_5706 0.31 -1.0 144,910 + Pf1N1B4_5706 0.33 -0.9 144,978 + Pf1N1B4_5706 0.39 -0.1 145,436 + Pf1N1B4_5706 0.80 +0.1 145,479 - Pf1N1B4_5706 0.84 +0.7 145,479 - Pf1N1B4_5706 0.84 +0.4 145,484 + Pf1N1B4_5706 0.84 -0.6 145,502 + Pf1N1B4_5706 0.86 -1.0 145,639 - -0.5 145,847 + Pf1N1B4_5707 0.16 -0.9 146,047 + Pf1N1B4_5707 0.34 -0.3 146,048 - Pf1N1B4_5707 0.35 -0.3 146,048 - Pf1N1B4_5707 0.35 -1.2 146,048 - -0.0 146,082 - Pf1N1B4_5707 0.38 -1.6 146,132 + Pf1N1B4_5707 0.42 +2.3 146,132 + Pf1N1B4_5707 0.42 +0.3 146,132 + Pf1N1B4_5707 0.42 -0.0 146,133 - Pf1N1B4_5707 0.42 +0.1 146,133 - Pf1N1B4_5707 0.42 +0.1 146,151 + Pf1N1B4_5707 0.44 +0.3 146,152 - Pf1N1B4_5707 0.44 -0.3 146,190 - Pf1N1B4_5707 0.48 +0.1 146,348 + Pf1N1B4_5707 0.62 -0.4 146,348 + Pf1N1B4_5707 0.62 +0.1 146,349 - +1.7 146,549 - -0.6 146,555 - Pf1N1B4_5707 0.81 +0.7 146,555 - Pf1N1B4_5707 0.81 +0.4 146,582 - Pf1N1B4_5707 0.83 +1.7 146,790 - -0.4 147,330 - Pf1N1B4_5708 0.79 +0.1 147,332 - Pf1N1B4_5708 0.79 -0.2 147,332 - Pf1N1B4_5708 0.79 -0.3 147,480 - +0.1 147,567 + -0.9 147,567 + -1.6 147,567 + -2.1 147,590 + -0.6 147,591 - -1.7 147,591 - -0.6 147,591 - -0.9 147,623 + +0.3 147,747 - +0.4
Or see this region's nucleotide sequence