Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4963

Experiment: D-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4962 and Pf1N1B4_4963 are separated by 233 nucleotidesPf1N1B4_4963 and Pf1N1B4_4964 are separated by 22 nucleotidesPf1N1B4_4964 and Pf1N1B4_4965 are separated by 24 nucleotides Pf1N1B4_4962 - C4-dicarboxylate transport protein, at 5,502,748 to 5,504,055 _4962 Pf1N1B4_4963 - Tricarboxylate transport protein TctC, at 5,504,289 to 5,505,254 _4963 Pf1N1B4_4964 - Tricarboxylate transport protein TctB, at 5,505,277 to 5,505,747 _4964 Pf1N1B4_4965 - Tricarboxylate transport membrane protein TctA, at 5,505,772 to 5,507,280 _4965 Position (kb) 5504 5505 5506Strain fitness (log2 ratio) -2 -1 0 1 2at 5503.613 kb on + strandat 5503.613 kb on + strand, within Pf1N1B4_4962at 5503.614 kb on - strand, within Pf1N1B4_4962at 5503.681 kb on + strand, within Pf1N1B4_4962at 5503.681 kb on + strand, within Pf1N1B4_4962at 5503.682 kb on - strand, within Pf1N1B4_4962at 5503.682 kb on - strand, within Pf1N1B4_4962at 5503.682 kb on - strand, within Pf1N1B4_4962at 5503.884 kb on + strand, within Pf1N1B4_4962at 5504.076 kb on + strandat 5504.076 kb on + strandat 5504.198 kb on + strandat 5504.198 kb on + strandat 5504.198 kb on + strandat 5504.199 kb on - strandat 5504.199 kb on - strandat 5504.199 kb on - strandat 5504.199 kb on - strandat 5504.205 kb on + strandat 5504.205 kb on + strandat 5504.205 kb on + strandat 5504.206 kb on - strandat 5504.206 kb on - strandat 5504.206 kb on - strandat 5504.211 kb on + strandat 5504.416 kb on - strand, within Pf1N1B4_4963at 5504.659 kb on - strand, within Pf1N1B4_4963at 5504.659 kb on - strand, within Pf1N1B4_4963at 5504.835 kb on + strand, within Pf1N1B4_4963at 5504.836 kb on - strand, within Pf1N1B4_4963at 5504.929 kb on - strand, within Pf1N1B4_4963at 5504.929 kb on - strand, within Pf1N1B4_4963at 5505.019 kb on - strand, within Pf1N1B4_4963at 5505.083 kb on + strand, within Pf1N1B4_4963at 5505.084 kb on - strand, within Pf1N1B4_4963at 5505.127 kb on + strand, within Pf1N1B4_4963at 5505.127 kb on + strand, within Pf1N1B4_4963at 5505.128 kb on - strand, within Pf1N1B4_4963at 5505.191 kb on + strandat 5505.302 kb on + strandat 5505.302 kb on + strandat 5505.303 kb on - strandat 5505.385 kb on + strand, within Pf1N1B4_4964at 5505.385 kb on + strand, within Pf1N1B4_4964at 5505.391 kb on + strand, within Pf1N1B4_4964at 5505.567 kb on + strand, within Pf1N1B4_4964at 5505.571 kb on + strand, within Pf1N1B4_4964at 5505.602 kb on + strand, within Pf1N1B4_4964at 5505.603 kb on - strand, within Pf1N1B4_4964at 5505.912 kb on - strandat 5505.976 kb on + strand, within Pf1N1B4_4965at 5505.976 kb on + strand, within Pf1N1B4_4965at 5506.210 kb on + strand, within Pf1N1B4_4965at 5506.211 kb on - strand, within Pf1N1B4_4965at 5506.211 kb on - strand, within Pf1N1B4_4965

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Lactate (C)
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5,503,613 + -0.2
5,503,613 + Pf1N1B4_4962 0.66 -2.0
5,503,614 - Pf1N1B4_4962 0.66 +1.8
5,503,681 + Pf1N1B4_4962 0.71 +0.8
5,503,681 + Pf1N1B4_4962 0.71 -0.6
5,503,682 - Pf1N1B4_4962 0.71 -1.2
5,503,682 - Pf1N1B4_4962 0.71 -0.5
5,503,682 - Pf1N1B4_4962 0.71 -0.2
5,503,884 + Pf1N1B4_4962 0.87 -0.0
5,504,076 + +0.5
5,504,076 + -0.8
5,504,198 + -0.4
5,504,198 + +0.1
5,504,198 + -2.5
5,504,199 - -1.8
5,504,199 - -0.8
5,504,199 - -0.8
5,504,199 - +0.2
5,504,205 + -0.5
5,504,205 + +0.1
5,504,205 + +0.9
5,504,206 - -1.8
5,504,206 - +1.1
5,504,206 - +0.4
5,504,211 + +0.1
5,504,416 - Pf1N1B4_4963 0.13 +1.4
5,504,659 - Pf1N1B4_4963 0.38 -1.4
5,504,659 - Pf1N1B4_4963 0.38 +0.4
5,504,835 + Pf1N1B4_4963 0.57 -0.8
5,504,836 - Pf1N1B4_4963 0.57 +0.6
5,504,929 - Pf1N1B4_4963 0.66 +0.1
5,504,929 - Pf1N1B4_4963 0.66 +0.3
5,505,019 - Pf1N1B4_4963 0.76 +0.2
5,505,083 + Pf1N1B4_4963 0.82 +0.6
5,505,084 - Pf1N1B4_4963 0.82 -0.4
5,505,127 + Pf1N1B4_4963 0.87 +1.2
5,505,127 + Pf1N1B4_4963 0.87 -0.2
5,505,128 - Pf1N1B4_4963 0.87 +0.6
5,505,191 + +2.4
5,505,302 + -0.8
5,505,302 + +0.5
5,505,303 - +0.5
5,505,385 + Pf1N1B4_4964 0.23 -0.8
5,505,385 + Pf1N1B4_4964 0.23 +0.3
5,505,391 + Pf1N1B4_4964 0.24 -0.3
5,505,567 + Pf1N1B4_4964 0.62 +0.8
5,505,571 + Pf1N1B4_4964 0.62 +0.1
5,505,602 + Pf1N1B4_4964 0.69 -0.2
5,505,603 - Pf1N1B4_4964 0.69 -0.9
5,505,912 - +0.5
5,505,976 + Pf1N1B4_4965 0.14 +0.1
5,505,976 + Pf1N1B4_4965 0.14 -0.1
5,506,210 + Pf1N1B4_4965 0.29 -2.2
5,506,211 - Pf1N1B4_4965 0.29 +0.7
5,506,211 - Pf1N1B4_4965 0.29 +0.8

Or see this region's nucleotide sequence