Experiment: D-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4192 and Pf1N1B4_4193 are separated by 47 nucleotides Pf1N1B4_4193 and Pf1N1B4_4194 are separated by 52 nucleotides Pf1N1B4_4194 and Pf1N1B4_4195 are separated by 146 nucleotides Pf1N1B4_4195 and Pf1N1B4_4196 overlap by 37 nucleotides Pf1N1B4_4196 and Pf1N1B4_4197 overlap by 2 nucleotides
Pf1N1B4_4192 - Hydrolase, alpha/beta fold family functionally coupled to Phosphoribulokinase, at 4,644,910 to 4,645,776
_4192
Pf1N1B4_4193 - Oxidoreductase, short chain dehydrogenase/reductase family, at 4,645,824 to 4,646,612
_4193
Pf1N1B4_4194 - 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34), at 4,646,665 to 4,647,786
_4194
Pf1N1B4_4195 - Transcriptional regulator, TetR family, at 4,647,933 to 4,648,505
_4195
Pf1N1B4_4196 - hypothetical protein, at 4,648,469 to 4,648,630
_4196
Pf1N1B4_4197 - Glucose ABC transport system, periplasmic sugar-binding protein, at 4,648,629 to 4,648,754
_4197
Position (kb) 4646
4647
4648 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4645.762 kb on - strand at 4645.841 kb on - strand at 4646.083 kb on + strand, within Pf1N1B4_4193 at 4646.083 kb on + strand, within Pf1N1B4_4193 at 4646.083 kb on + strand at 4646.084 kb on - strand, within Pf1N1B4_4193 at 4646.084 kb on - strand, within Pf1N1B4_4193 at 4646.084 kb on - strand, within Pf1N1B4_4193 at 4646.084 kb on - strand, within Pf1N1B4_4193 at 4646.166 kb on - strand, within Pf1N1B4_4193 at 4646.598 kb on - strand at 4646.732 kb on + strand at 4646.733 kb on - strand at 4646.733 kb on - strand at 4646.753 kb on + strand at 4646.869 kb on - strand at 4646.915 kb on + strand, within Pf1N1B4_4194 at 4646.915 kb on + strand, within Pf1N1B4_4194 at 4646.915 kb on + strand, within Pf1N1B4_4194 at 4646.915 kb on + strand, within Pf1N1B4_4194 at 4646.915 kb on + strand at 4646.916 kb on - strand, within Pf1N1B4_4194 at 4646.916 kb on - strand at 4646.916 kb on - strand at 4646.916 kb on - strand, within Pf1N1B4_4194 at 4647.246 kb on - strand, within Pf1N1B4_4194 at 4647.563 kb on + strand at 4647.657 kb on - strand, within Pf1N1B4_4194 at 4647.766 kb on - strand at 4647.821 kb on - strand at 4647.886 kb on + strand at 4647.887 kb on - strand at 4647.890 kb on + strand at 4648.185 kb on + strand at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand at 4648.186 kb on - strand, within Pf1N1B4_4195 at 4648.186 kb on - strand, within Pf1N1B4_4195 at 4648.186 kb on - strand, within Pf1N1B4_4195 at 4648.186 kb on - strand at 4648.186 kb on - strand, within Pf1N1B4_4195 at 4648.186 kb on - strand, within Pf1N1B4_4195 at 4648.329 kb on + strand, within Pf1N1B4_4195
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lactate (C) remove 4,645,762 - +0.2 4,645,841 - -0.8 4,646,083 + Pf1N1B4_4193 0.33 +0.7 4,646,083 + Pf1N1B4_4193 0.33 +0.5 4,646,083 + +0.8 4,646,084 - Pf1N1B4_4193 0.33 +0.1 4,646,084 - Pf1N1B4_4193 0.33 -0.2 4,646,084 - Pf1N1B4_4193 0.33 +0.2 4,646,084 - Pf1N1B4_4193 0.33 -0.8 4,646,166 - Pf1N1B4_4193 0.43 +0.1 4,646,598 - -0.2 4,646,732 + +0.3 4,646,733 - +0.0 4,646,733 - -0.2 4,646,753 + -1.0 4,646,869 - +0.8 4,646,915 + Pf1N1B4_4194 0.22 -0.5 4,646,915 + Pf1N1B4_4194 0.22 +1.1 4,646,915 + Pf1N1B4_4194 0.22 +1.8 4,646,915 + Pf1N1B4_4194 0.22 +0.7 4,646,915 + +0.2 4,646,916 - Pf1N1B4_4194 0.22 +1.1 4,646,916 - -0.2 4,646,916 - +0.2 4,646,916 - Pf1N1B4_4194 0.22 +0.6 4,647,246 - Pf1N1B4_4194 0.52 +0.0 4,647,563 + -0.2 4,647,657 - Pf1N1B4_4194 0.88 -0.7 4,647,766 - -0.2 4,647,821 - +0.0 4,647,886 + +0.3 4,647,887 - +0.2 4,647,890 + -0.2 4,648,185 + +0.8 4,648,185 + Pf1N1B4_4195 0.44 -2.0 4,648,185 + Pf1N1B4_4195 0.44 -1.2 4,648,185 + Pf1N1B4_4195 0.44 +2.1 4,648,185 + Pf1N1B4_4195 0.44 -0.4 4,648,185 + Pf1N1B4_4195 0.44 -0.5 4,648,185 + Pf1N1B4_4195 0.44 -1.1 4,648,185 + -0.5 4,648,185 + Pf1N1B4_4195 0.44 -0.4 4,648,185 + -0.2 4,648,186 - Pf1N1B4_4195 0.44 -1.0 4,648,186 - Pf1N1B4_4195 0.44 +0.1 4,648,186 - Pf1N1B4_4195 0.44 +1.2 4,648,186 - +0.8 4,648,186 - Pf1N1B4_4195 0.44 +0.1 4,648,186 - Pf1N1B4_4195 0.44 +0.8 4,648,329 + Pf1N1B4_4195 0.69 -1.8
Or see this region's nucleotide sequence