Experiment: D-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4030 and Pf1N1B4_4031 overlap by 4 nucleotides Pf1N1B4_4031 and Pf1N1B4_4032 are separated by 2 nucleotides Pf1N1B4_4032 and Pf1N1B4_4033 are separated by 9 nucleotides Pf1N1B4_4033 and Pf1N1B4_4034 are separated by 12 nucleotides
Pf1N1B4_4030 - NADH-ubiquinone oxidoreductase chain L (EC 1.6.5.3), at 4,481,295 to 4,483,148
_4030
Pf1N1B4_4031 - NADH-ubiquinone oxidoreductase chain K (EC 1.6.5.3), at 4,483,145 to 4,483,453
_4031
Pf1N1B4_4032 - NADH-ubiquinone oxidoreductase chain J (EC 1.6.5.3), at 4,483,456 to 4,483,959
_4032
Pf1N1B4_4033 - NADH-ubiquinone oxidoreductase chain I (EC 1.6.5.3), at 4,483,969 to 4,484,514
_4033
Pf1N1B4_4034 - NADH-ubiquinone oxidoreductase chain H (EC 1.6.5.3), at 4,484,527 to 4,485,534
_4034
Position (kb)
4483
4484
4485 Strain fitness (log2 ratio)
-1
0
1 at 4483.005 kb on + strand at 4483.006 kb on - strand at 4483.198 kb on - strand, within Pf1N1B4_4031 at 4483.634 kb on + strand, within Pf1N1B4_4032 at 4483.635 kb on - strand, within Pf1N1B4_4032 at 4484.060 kb on + strand, within Pf1N1B4_4033 at 4484.061 kb on - strand at 4484.069 kb on + strand, within Pf1N1B4_4033 at 4484.070 kb on - strand, within Pf1N1B4_4033 at 4484.070 kb on - strand, within Pf1N1B4_4033 at 4484.508 kb on - strand at 4484.508 kb on - strand at 4484.807 kb on + strand, within Pf1N1B4_4034 at 4485.124 kb on - strand, within Pf1N1B4_4034 at 4485.503 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lactate (C) remove 4,483,005 + -0.8 4,483,006 - +0.2 4,483,198 - Pf1N1B4_4031 0.17 -0.5 4,483,634 + Pf1N1B4_4032 0.35 +0.1 4,483,635 - Pf1N1B4_4032 0.36 +1.7 4,484,060 + Pf1N1B4_4033 0.17 +1.2 4,484,061 - -0.7 4,484,069 + Pf1N1B4_4033 0.18 -1.3 4,484,070 - Pf1N1B4_4033 0.18 -0.7 4,484,070 - Pf1N1B4_4033 0.18 -0.2 4,484,508 - -0.5 4,484,508 - -0.9 4,484,807 + Pf1N1B4_4034 0.28 +0.6 4,485,124 - Pf1N1B4_4034 0.59 -0.8 4,485,503 - -0.4
Or see this region's nucleotide sequence