Experiment: D-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_356 and Pf1N1B4_357 are separated by 4 nucleotides Pf1N1B4_357 and Pf1N1B4_358 are separated by 186 nucleotides Pf1N1B4_358 and Pf1N1B4_359 are separated by 374 nucleotides
Pf1N1B4_356 - 3-demethylubiquinone-9 3-methyltransferase (EC 2.1.1.64), at 423,359 to 424,057
_356
Pf1N1B4_357 - Similar to phosphoglycolate phosphatase, clustered with ubiquinone biosynthesis SAM-dependent O-methyltransferase, at 424,062 to 424,733
_357
Pf1N1B4_358 - Oxidoreductase, short chain dehydrogenase/reductase family, at 424,920 to 425,660
_358
Pf1N1B4_359 - GGDEF domain protein, at 426,035 to 426,961
_359
Position (kb) 424
425
426 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 424.630 kb on - strand, within Pf1N1B4_357 at 424.793 kb on + strand at 424.793 kb on + strand at 424.793 kb on + strand at 424.794 kb on - strand at 424.794 kb on - strand at 424.794 kb on - strand at 424.794 kb on - strand at 424.797 kb on + strand at 424.797 kb on + strand at 424.797 kb on + strand at 424.797 kb on + strand at 424.797 kb on + strand at 424.797 kb on + strand at 424.797 kb on + strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.838 kb on - strand at 424.883 kb on + strand at 424.883 kb on + strand at 424.883 kb on + strand at 424.883 kb on + strand at 424.883 kb on + strand at 424.919 kb on + strand at 424.919 kb on + strand at 424.920 kb on - strand at 424.920 kb on - strand at 424.920 kb on - strand at 424.930 kb on - strand at 424.930 kb on - strand at 425.017 kb on - strand, within Pf1N1B4_358 at 425.017 kb on - strand at 425.079 kb on + strand, within Pf1N1B4_358 at 425.079 kb on + strand, within Pf1N1B4_358 at 425.079 kb on + strand, within Pf1N1B4_358 at 425.079 kb on + strand, within Pf1N1B4_358 at 425.080 kb on - strand, within Pf1N1B4_358 at 425.080 kb on - strand, within Pf1N1B4_358 at 425.080 kb on - strand, within Pf1N1B4_358 at 425.080 kb on - strand, within Pf1N1B4_358 at 425.157 kb on + strand, within Pf1N1B4_358 at 425.157 kb on + strand, within Pf1N1B4_358 at 425.157 kb on + strand, within Pf1N1B4_358 at 425.157 kb on + strand, within Pf1N1B4_358 at 425.157 kb on + strand, within Pf1N1B4_358 at 425.157 kb on + strand at 425.157 kb on + strand, within Pf1N1B4_358 at 425.158 kb on - strand, within Pf1N1B4_358 at 425.158 kb on - strand, within Pf1N1B4_358 at 425.158 kb on - strand, within Pf1N1B4_358 at 425.158 kb on - strand, within Pf1N1B4_358 at 425.158 kb on - strand, within Pf1N1B4_358 at 425.470 kb on + strand, within Pf1N1B4_358 at 425.470 kb on + strand, within Pf1N1B4_358 at 425.470 kb on + strand, within Pf1N1B4_358 at 425.470 kb on + strand, within Pf1N1B4_358 at 425.470 kb on + strand, within Pf1N1B4_358 at 425.470 kb on + strand, within Pf1N1B4_358 at 425.471 kb on - strand, within Pf1N1B4_358 at 425.471 kb on - strand, within Pf1N1B4_358 at 425.471 kb on - strand, within Pf1N1B4_358 at 425.471 kb on - strand, within Pf1N1B4_358 at 425.471 kb on - strand, within Pf1N1B4_358 at 425.471 kb on - strand, within Pf1N1B4_358 at 425.471 kb on - strand, within Pf1N1B4_358 at 425.544 kb on + strand, within Pf1N1B4_358 at 425.598 kb on + strand at 425.598 kb on + strand at 425.598 kb on + strand at 425.599 kb on - strand at 425.599 kb on - strand at 425.604 kb on + strand at 425.604 kb on + strand at 425.604 kb on + strand at 425.604 kb on + strand at 425.604 kb on + strand at 425.604 kb on + strand at 425.605 kb on - strand at 425.605 kb on - strand at 425.658 kb on + strand at 425.721 kb on - strand at 425.745 kb on - strand at 425.745 kb on - strand at 425.745 kb on - strand at 425.905 kb on + strand at 425.905 kb on + strand at 425.906 kb on - strand at 425.925 kb on + strand at 425.925 kb on + strand at 426.001 kb on + strand at 426.012 kb on + strand at 426.013 kb on - strand at 426.211 kb on - strand, within Pf1N1B4_359 at 426.317 kb on + strand, within Pf1N1B4_359 at 426.620 kb on + strand, within Pf1N1B4_359 at 426.620 kb on + strand, within Pf1N1B4_359 at 426.620 kb on + strand, within Pf1N1B4_359 at 426.621 kb on - strand, within Pf1N1B4_359
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lactate (C) remove 424,630 - Pf1N1B4_357 0.85 +0.6 424,793 + +0.6 424,793 + -0.2 424,793 + -0.6 424,794 - -1.8 424,794 - -0.2 424,794 - -0.2 424,794 - +0.0 424,797 + +1.6 424,797 + -1.3 424,797 + +0.3 424,797 + +0.1 424,797 + +0.7 424,797 + -1.6 424,797 + -0.4 424,798 - -0.5 424,798 - +0.0 424,798 - +0.0 424,798 - +0.0 424,798 - +0.6 424,798 - -0.6 424,798 - -2.2 424,798 - +2.0 424,798 - +0.0 424,798 - -0.6 424,798 - +0.3 424,798 - -0.3 424,798 - -0.2 424,838 - -0.6 424,883 + -0.7 424,883 + +0.4 424,883 + +0.4 424,883 + -0.7 424,883 + -1.3 424,919 + +0.4 424,919 + -0.4 424,920 - -1.0 424,920 - +0.0 424,920 - -1.3 424,930 - -2.9 424,930 - -1.0 425,017 - Pf1N1B4_358 0.13 +0.0 425,017 - +0.0 425,079 + Pf1N1B4_358 0.21 -0.7 425,079 + Pf1N1B4_358 0.21 -0.7 425,079 + Pf1N1B4_358 0.21 -0.7 425,079 + Pf1N1B4_358 0.21 -3.2 425,080 - Pf1N1B4_358 0.22 -1.6 425,080 - Pf1N1B4_358 0.22 -0.4 425,080 - Pf1N1B4_358 0.22 +0.6 425,080 - Pf1N1B4_358 0.22 -1.5 425,157 + Pf1N1B4_358 0.32 +1.0 425,157 + Pf1N1B4_358 0.32 -0.4 425,157 + Pf1N1B4_358 0.32 -1.5 425,157 + Pf1N1B4_358 0.32 -0.8 425,157 + Pf1N1B4_358 0.32 -1.0 425,157 + +1.0 425,157 + Pf1N1B4_358 0.32 +0.4 425,158 - Pf1N1B4_358 0.32 -1.9 425,158 - Pf1N1B4_358 0.32 +1.0 425,158 - Pf1N1B4_358 0.32 -0.3 425,158 - Pf1N1B4_358 0.32 +1.0 425,158 - Pf1N1B4_358 0.32 -1.0 425,470 + Pf1N1B4_358 0.74 -1.3 425,470 + Pf1N1B4_358 0.74 -0.7 425,470 + Pf1N1B4_358 0.74 -0.8 425,470 + Pf1N1B4_358 0.74 -2.0 425,470 + Pf1N1B4_358 0.74 -0.8 425,470 + Pf1N1B4_358 0.74 -0.7 425,471 - Pf1N1B4_358 0.74 -1.0 425,471 - Pf1N1B4_358 0.74 -3.0 425,471 - Pf1N1B4_358 0.74 -2.5 425,471 - Pf1N1B4_358 0.74 -0.6 425,471 - Pf1N1B4_358 0.74 +0.0 425,471 - Pf1N1B4_358 0.74 -0.7 425,471 - Pf1N1B4_358 0.74 +1.0 425,544 + Pf1N1B4_358 0.84 -1.6 425,598 + -0.7 425,598 + -0.6 425,598 + -1.0 425,599 - +0.4 425,599 - -0.4 425,604 + +0.6 425,604 + -2.5 425,604 + +0.3 425,604 + -0.8 425,604 + -2.0 425,604 + -2.0 425,605 - -0.2 425,605 - -2.5 425,658 + -0.5 425,721 - -0.7 425,745 - +0.1 425,745 - -0.7 425,745 - +0.0 425,905 + -0.8 425,905 + +0.0 425,906 - +0.3 425,925 + +1.0 425,925 + -1.3 426,001 + +1.0 426,012 + -1.3 426,013 - +0.0 426,211 - Pf1N1B4_359 0.19 -1.8 426,317 + Pf1N1B4_359 0.30 +0.7 426,620 + Pf1N1B4_359 0.63 -0.7 426,620 + Pf1N1B4_359 0.63 -0.2 426,620 + Pf1N1B4_359 0.63 +0.6 426,621 - Pf1N1B4_359 0.63 +0.7
Or see this region's nucleotide sequence