Experiment: R2A with Cisplatin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS22550 and CA265_RS22555 overlap by 1 nucleotides CA265_RS22555 and CA265_RS22560 are separated by 11 nucleotides CA265_RS22560 and CA265_RS22565 are separated by 71 nucleotides
CA265_RS22550: CA265_RS22550 - hypothetical protein, at 5,525,972 to 5,527,030
_RS22550
CA265_RS22555: CA265_RS22555 - lipoyl(octanoyl) transferase, at 5,527,030 to 5,527,758
_RS22555
CA265_RS22560: CA265_RS22560 - 4'-phosphopantetheinyl transferase, at 5,527,770 to 5,528,417
_RS22560
CA265_RS22565: CA265_RS22565 - dCTP deaminase, at 5,528,489 to 5,529,025
_RS22565
Position (kb)
5527
5528
5529 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5526.778 kb on + strand, within CA265_RS22550 at 5526.779 kb on - strand, within CA265_RS22550 at 5526.882 kb on + strand, within CA265_RS22550 at 5526.883 kb on - strand, within CA265_RS22550 at 5526.890 kb on + strand, within CA265_RS22550 at 5526.914 kb on + strand, within CA265_RS22550 at 5526.915 kb on - strand, within CA265_RS22550 at 5526.991 kb on + strand at 5526.991 kb on + strand at 5526.991 kb on + strand at 5526.991 kb on + strand at 5527.002 kb on - strand at 5527.031 kb on + strand at 5527.767 kb on - strand at 5527.785 kb on + strand at 5527.785 kb on + strand at 5527.795 kb on - strand at 5528.434 kb on + strand at 5528.455 kb on + strand at 5528.463 kb on - strand at 5528.569 kb on - strand, within CA265_RS22565 at 5528.603 kb on + strand, within CA265_RS22565 at 5528.611 kb on + strand, within CA265_RS22565 at 5528.617 kb on + strand, within CA265_RS22565 at 5528.618 kb on - strand, within CA265_RS22565 at 5528.675 kb on - strand, within CA265_RS22565 at 5528.685 kb on - strand, within CA265_RS22565 at 5528.685 kb on - strand, within CA265_RS22565 at 5528.686 kb on + strand, within CA265_RS22565 at 5528.686 kb on + strand, within CA265_RS22565 at 5528.686 kb on + strand, within CA265_RS22565 at 5528.686 kb on + strand, within CA265_RS22565 at 5528.687 kb on - strand, within CA265_RS22565 at 5528.707 kb on - strand, within CA265_RS22565 at 5528.724 kb on + strand, within CA265_RS22565 at 5528.729 kb on + strand, within CA265_RS22565 at 5528.751 kb on + strand, within CA265_RS22565 at 5528.767 kb on + strand, within CA265_RS22565 at 5528.767 kb on + strand, within CA265_RS22565 at 5528.776 kb on + strand, within CA265_RS22565 at 5528.815 kb on + strand, within CA265_RS22565 at 5528.837 kb on - strand, within CA265_RS22565 at 5528.945 kb on + strand, within CA265_RS22565 at 5528.946 kb on - strand, within CA265_RS22565 at 5528.965 kb on + strand, within CA265_RS22565 at 5528.965 kb on + strand, within CA265_RS22565 at 5528.965 kb on + strand, within CA265_RS22565 at 5528.966 kb on - strand, within CA265_RS22565 at 5528.969 kb on + strand, within CA265_RS22565 at 5528.977 kb on + strand at 5528.978 kb on - strand at 5529.017 kb on - strand at 5529.085 kb on + strand at 5529.086 kb on - strand at 5529.106 kb on + strand at 5529.156 kb on - strand at 5529.220 kb on + strand at 5529.220 kb on + strand at 5529.331 kb on + strand at 5529.332 kb on - strand at 5529.332 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.01 mg/ml remove 5,526,778 + CA265_RS22550 0.76 -0.5 5,526,779 - CA265_RS22550 0.76 -0.2 5,526,882 + CA265_RS22550 0.86 +0.3 5,526,883 - CA265_RS22550 0.86 -1.0 5,526,890 + CA265_RS22550 0.87 -1.6 5,526,914 + CA265_RS22550 0.89 +0.1 5,526,915 - CA265_RS22550 0.89 -0.2 5,526,991 + -0.1 5,526,991 + +0.2 5,526,991 + -1.9 5,526,991 + +0.1 5,527,002 - +0.6 5,527,031 + +0.5 5,527,767 - +0.2 5,527,785 + +0.4 5,527,785 + -0.6 5,527,795 - -2.0 5,528,434 + -1.3 5,528,455 + -0.1 5,528,463 - +0.3 5,528,569 - CA265_RS22565 0.15 -0.8 5,528,603 + CA265_RS22565 0.21 +0.2 5,528,611 + CA265_RS22565 0.23 -0.8 5,528,617 + CA265_RS22565 0.24 -0.6 5,528,618 - CA265_RS22565 0.24 -1.2 5,528,675 - CA265_RS22565 0.35 -0.5 5,528,685 - CA265_RS22565 0.36 -3.1 5,528,685 - CA265_RS22565 0.36 -0.1 5,528,686 + CA265_RS22565 0.37 -0.1 5,528,686 + CA265_RS22565 0.37 -1.3 5,528,686 + CA265_RS22565 0.37 -0.7 5,528,686 + CA265_RS22565 0.37 -1.7 5,528,687 - CA265_RS22565 0.37 +0.4 5,528,707 - CA265_RS22565 0.41 -0.8 5,528,724 + CA265_RS22565 0.44 -0.0 5,528,729 + CA265_RS22565 0.45 -0.4 5,528,751 + CA265_RS22565 0.49 +0.6 5,528,767 + CA265_RS22565 0.52 -0.7 5,528,767 + CA265_RS22565 0.52 +0.1 5,528,776 + CA265_RS22565 0.53 +0.9 5,528,815 + CA265_RS22565 0.61 -2.1 5,528,837 - CA265_RS22565 0.65 -0.4 5,528,945 + CA265_RS22565 0.85 -0.5 5,528,946 - CA265_RS22565 0.85 -0.2 5,528,965 + CA265_RS22565 0.89 -2.3 5,528,965 + CA265_RS22565 0.89 -0.9 5,528,965 + CA265_RS22565 0.89 -1.8 5,528,966 - CA265_RS22565 0.89 +1.2 5,528,969 + CA265_RS22565 0.89 -0.6 5,528,977 + -0.2 5,528,978 - -0.8 5,529,017 - -0.4 5,529,085 + -2.3 5,529,086 - +0.5 5,529,106 + +0.8 5,529,156 - -0.9 5,529,220 + -0.0 5,529,220 + +0.1 5,529,331 + -0.9 5,529,332 - +2.0 5,529,332 - -0.5
Or see this region's nucleotide sequence