Experiment: R2A with Cisplatin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS20645 and CA265_RS20650 are separated by 150 nucleotides CA265_RS20650 and CA265_RS20655 are separated by 75 nucleotides CA265_RS20655 and CA265_RS20660 are separated by 91 nucleotides CA265_RS20660 and CA265_RS20665 are separated by 317 nucleotides
CA265_RS20645: CA265_RS20645 - RNA polymerase subunit sigma-70, at 5,110,548 to 5,111,126
_RS20645
CA265_RS20650: CA265_RS20650 - hypothetical protein, at 5,111,277 to 5,111,528
_RS20650
CA265_RS20655: CA265_RS20655 - transcriptional regulator, at 5,111,604 to 5,111,873
_RS20655
CA265_RS20660: CA265_RS20660 - hypothetical protein, at 5,111,965 to 5,112,837
_RS20660
CA265_RS20665: CA265_RS20665 - hypothetical protein, at 5,113,155 to 5,114,132
_RS20665
Position (kb)
5111
5112
5113 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5111.031 kb on - strand, within CA265_RS20645 at 5111.035 kb on + strand, within CA265_RS20645 at 5111.035 kb on + strand, within CA265_RS20645 at 5111.089 kb on + strand at 5111.112 kb on - strand at 5111.112 kb on - strand at 5111.114 kb on + strand at 5111.116 kb on + strand at 5111.116 kb on + strand at 5111.191 kb on + strand at 5111.218 kb on - strand at 5111.234 kb on - strand at 5111.237 kb on + strand at 5111.352 kb on + strand, within CA265_RS20650 at 5111.426 kb on + strand, within CA265_RS20650 at 5111.504 kb on - strand at 5111.506 kb on - strand at 5111.548 kb on - strand at 5111.558 kb on - strand at 5111.579 kb on + strand at 5111.580 kb on - strand at 5111.603 kb on + strand at 5111.610 kb on - strand at 5111.718 kb on + strand, within CA265_RS20655 at 5111.724 kb on - strand, within CA265_RS20655 at 5111.724 kb on - strand, within CA265_RS20655 at 5111.733 kb on + strand, within CA265_RS20655 at 5111.769 kb on + strand, within CA265_RS20655 at 5111.769 kb on + strand, within CA265_RS20655 at 5111.770 kb on - strand, within CA265_RS20655 at 5111.770 kb on - strand, within CA265_RS20655 at 5111.770 kb on - strand, within CA265_RS20655 at 5111.770 kb on - strand, within CA265_RS20655 at 5111.789 kb on - strand, within CA265_RS20655 at 5111.801 kb on + strand, within CA265_RS20655 at 5111.801 kb on + strand, within CA265_RS20655 at 5111.868 kb on - strand at 5111.959 kb on + strand at 5111.963 kb on + strand at 5111.964 kb on - strand at 5111.978 kb on - strand at 5111.978 kb on - strand at 5111.992 kb on + strand at 5112.025 kb on + strand at 5112.025 kb on + strand at 5112.025 kb on + strand at 5112.026 kb on - strand at 5112.245 kb on - strand, within CA265_RS20660 at 5112.248 kb on - strand, within CA265_RS20660 at 5112.307 kb on + strand, within CA265_RS20660 at 5112.387 kb on - strand, within CA265_RS20660 at 5112.421 kb on + strand, within CA265_RS20660 at 5112.422 kb on - strand, within CA265_RS20660 at 5112.490 kb on + strand, within CA265_RS20660 at 5112.491 kb on - strand, within CA265_RS20660 at 5112.491 kb on - strand, within CA265_RS20660 at 5112.494 kb on + strand, within CA265_RS20660 at 5112.495 kb on - strand, within CA265_RS20660 at 5112.631 kb on + strand, within CA265_RS20660 at 5112.631 kb on + strand, within CA265_RS20660 at 5112.631 kb on + strand, within CA265_RS20660 at 5112.737 kb on - strand, within CA265_RS20660 at 5112.879 kb on - strand at 5112.901 kb on - strand at 5113.053 kb on - strand at 5113.089 kb on - strand at 5113.100 kb on - strand at 5113.147 kb on - strand at 5113.147 kb on - strand at 5113.150 kb on - strand at 5113.155 kb on - strand at 5113.155 kb on - strand at 5113.414 kb on - strand, within CA265_RS20665 at 5113.475 kb on + strand, within CA265_RS20665 at 5113.476 kb on - strand, within CA265_RS20665 at 5113.481 kb on + strand, within CA265_RS20665 at 5113.643 kb on - strand, within CA265_RS20665 at 5113.792 kb on + strand, within CA265_RS20665
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.01 mg/ml remove 5,111,031 - CA265_RS20645 0.83 +0.2 5,111,035 + CA265_RS20645 0.84 +0.3 5,111,035 + CA265_RS20645 0.84 +0.6 5,111,089 + -0.5 5,111,112 - +0.7 5,111,112 - +1.3 5,111,114 + -0.9 5,111,116 + -0.0 5,111,116 + -2.5 5,111,191 + +0.5 5,111,218 - +0.2 5,111,234 - -0.9 5,111,237 + -0.5 5,111,352 + CA265_RS20650 0.30 +1.0 5,111,426 + CA265_RS20650 0.59 +1.5 5,111,504 - -0.2 5,111,506 - +0.0 5,111,548 - -0.5 5,111,558 - +0.5 5,111,579 + -0.8 5,111,580 - -0.3 5,111,603 + -0.5 5,111,610 - -0.7 5,111,718 + CA265_RS20655 0.42 -0.4 5,111,724 - CA265_RS20655 0.44 -0.2 5,111,724 - CA265_RS20655 0.44 +0.2 5,111,733 + CA265_RS20655 0.48 +0.0 5,111,769 + CA265_RS20655 0.61 +0.5 5,111,769 + CA265_RS20655 0.61 +0.2 5,111,770 - CA265_RS20655 0.61 +0.5 5,111,770 - CA265_RS20655 0.61 +1.1 5,111,770 - CA265_RS20655 0.61 +1.1 5,111,770 - CA265_RS20655 0.61 -0.4 5,111,789 - CA265_RS20655 0.69 +0.2 5,111,801 + CA265_RS20655 0.73 +0.9 5,111,801 + CA265_RS20655 0.73 -0.1 5,111,868 - +0.5 5,111,959 + -0.6 5,111,963 + +0.8 5,111,964 - +0.5 5,111,978 - -0.1 5,111,978 - +0.3 5,111,992 + -0.5 5,112,025 + -1.0 5,112,025 + +2.1 5,112,025 + +0.7 5,112,026 - -0.2 5,112,245 - CA265_RS20660 0.32 -0.3 5,112,248 - CA265_RS20660 0.32 -0.9 5,112,307 + CA265_RS20660 0.39 -0.9 5,112,387 - CA265_RS20660 0.48 -0.1 5,112,421 + CA265_RS20660 0.52 +0.1 5,112,422 - CA265_RS20660 0.52 -0.9 5,112,490 + CA265_RS20660 0.60 +0.3 5,112,491 - CA265_RS20660 0.60 +0.7 5,112,491 - CA265_RS20660 0.60 +1.4 5,112,494 + CA265_RS20660 0.61 -0.6 5,112,495 - CA265_RS20660 0.61 -1.9 5,112,631 + CA265_RS20660 0.76 +0.4 5,112,631 + CA265_RS20660 0.76 -1.4 5,112,631 + CA265_RS20660 0.76 -2.1 5,112,737 - CA265_RS20660 0.88 -1.7 5,112,879 - -0.3 5,112,901 - +0.3 5,113,053 - +0.0 5,113,089 - +0.2 5,113,100 - -1.0 5,113,147 - -0.2 5,113,147 - -0.1 5,113,150 - -0.0 5,113,155 - -0.6 5,113,155 - -0.4 5,113,414 - CA265_RS20665 0.26 -0.5 5,113,475 + CA265_RS20665 0.33 +2.1 5,113,476 - CA265_RS20665 0.33 +1.8 5,113,481 + CA265_RS20665 0.33 +0.1 5,113,643 - CA265_RS20665 0.50 -0.7 5,113,792 + CA265_RS20665 0.65 -0.0
Or see this region's nucleotide sequence