Experiment: R2A with Cisplatin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS12980 and CA265_RS12985 are separated by 203 nucleotides CA265_RS12985 and CA265_RS12990 are separated by 126 nucleotides CA265_RS12990 and CA265_RS12995 are separated by 288 nucleotides CA265_RS12995 and CA265_RS13000 overlap by 1 nucleotides CA265_RS13000 and CA265_RS13005 are separated by 12 nucleotides
CA265_RS12980: CA265_RS12980 - ATP-dependent DNA helicase, at 3,230,513 to 3,232,816
_RS12980
CA265_RS12985: CA265_RS12985 - hypothetical protein, at 3,233,020 to 3,233,439
_RS12985
CA265_RS12990: CA265_RS12990 - chromosome segregation protein SMC, at 3,233,566 to 3,237,105
_RS12990
CA265_RS12995: CA265_RS12995 - hypothetical protein, at 3,237,394 to 3,237,603
_RS12995
CA265_RS13000: CA265_RS13000 - hypothetical protein, at 3,237,603 to 3,238,013
_RS13000
CA265_RS13005: CA265_RS13005 - peptidase M20, at 3,238,026 to 3,239,000
_RS13005
Position (kb)
3233
3234
3235
3236
3237
3238 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3232.893 kb on - strand at 3232.911 kb on + strand at 3232.919 kb on - strand at 3232.919 kb on - strand at 3232.928 kb on + strand at 3232.983 kb on + strand at 3233.091 kb on + strand, within CA265_RS12985 at 3233.092 kb on - strand, within CA265_RS12985 at 3233.124 kb on + strand, within CA265_RS12985 at 3233.125 kb on - strand, within CA265_RS12985 at 3233.270 kb on + strand, within CA265_RS12985 at 3233.483 kb on - strand at 3233.575 kb on - strand at 3233.577 kb on + strand at 3233.577 kb on + strand at 3233.599 kb on - strand at 3233.649 kb on + strand at 3233.685 kb on - strand at 3233.820 kb on + strand at 3233.871 kb on + strand at 3233.872 kb on - strand at 3233.880 kb on + strand at 3233.973 kb on + strand, within CA265_RS12990 at 3234.044 kb on + strand, within CA265_RS12990 at 3234.081 kb on - strand, within CA265_RS12990 at 3234.196 kb on - strand, within CA265_RS12990 at 3234.211 kb on + strand, within CA265_RS12990 at 3234.229 kb on + strand, within CA265_RS12990 at 3234.273 kb on - strand, within CA265_RS12990 at 3234.288 kb on - strand, within CA265_RS12990 at 3234.393 kb on + strand, within CA265_RS12990 at 3234.393 kb on + strand, within CA265_RS12990 at 3234.571 kb on - strand, within CA265_RS12990 at 3234.574 kb on + strand, within CA265_RS12990 at 3234.591 kb on - strand, within CA265_RS12990 at 3234.749 kb on + strand, within CA265_RS12990 at 3234.749 kb on + strand, within CA265_RS12990 at 3234.750 kb on - strand, within CA265_RS12990 at 3234.750 kb on - strand, within CA265_RS12990 at 3234.750 kb on - strand, within CA265_RS12990 at 3234.750 kb on - strand, within CA265_RS12990 at 3234.848 kb on + strand, within CA265_RS12990 at 3234.888 kb on + strand, within CA265_RS12990 at 3234.918 kb on + strand, within CA265_RS12990 at 3234.981 kb on - strand, within CA265_RS12990 at 3234.981 kb on - strand, within CA265_RS12990 at 3235.042 kb on + strand, within CA265_RS12990 at 3235.044 kb on + strand, within CA265_RS12990 at 3235.045 kb on - strand, within CA265_RS12990 at 3235.185 kb on - strand, within CA265_RS12990 at 3235.329 kb on + strand, within CA265_RS12990 at 3235.370 kb on + strand, within CA265_RS12990 at 3235.371 kb on - strand, within CA265_RS12990 at 3235.371 kb on - strand, within CA265_RS12990 at 3235.504 kb on + strand, within CA265_RS12990 at 3235.505 kb on - strand, within CA265_RS12990 at 3235.626 kb on + strand, within CA265_RS12990 at 3235.635 kb on + strand, within CA265_RS12990 at 3235.647 kb on - strand, within CA265_RS12990 at 3235.667 kb on + strand, within CA265_RS12990 at 3235.667 kb on + strand, within CA265_RS12990 at 3235.929 kb on + strand, within CA265_RS12990 at 3235.929 kb on + strand, within CA265_RS12990 at 3235.976 kb on - strand, within CA265_RS12990 at 3235.976 kb on - strand, within CA265_RS12990 at 3235.976 kb on - strand, within CA265_RS12990 at 3236.039 kb on + strand, within CA265_RS12990 at 3236.135 kb on + strand, within CA265_RS12990 at 3236.135 kb on + strand, within CA265_RS12990 at 3236.135 kb on + strand, within CA265_RS12990 at 3236.136 kb on - strand, within CA265_RS12990 at 3236.176 kb on + strand, within CA265_RS12990 at 3236.227 kb on + strand, within CA265_RS12990 at 3236.250 kb on + strand, within CA265_RS12990 at 3236.251 kb on - strand, within CA265_RS12990 at 3236.309 kb on + strand, within CA265_RS12990 at 3236.375 kb on + strand, within CA265_RS12990 at 3236.508 kb on + strand, within CA265_RS12990 at 3236.533 kb on - strand, within CA265_RS12990 at 3236.552 kb on - strand, within CA265_RS12990 at 3236.584 kb on - strand, within CA265_RS12990 at 3236.617 kb on - strand, within CA265_RS12990 at 3236.702 kb on + strand, within CA265_RS12990 at 3236.703 kb on - strand, within CA265_RS12990 at 3236.719 kb on - strand, within CA265_RS12990 at 3236.766 kb on + strand at 3236.775 kb on + strand at 3236.778 kb on - strand at 3236.869 kb on + strand at 3236.955 kb on + strand at 3237.001 kb on - strand at 3237.030 kb on + strand at 3237.030 kb on + strand at 3237.074 kb on + strand at 3237.095 kb on + strand at 3237.104 kb on - strand at 3237.239 kb on + strand at 3237.266 kb on - strand at 3237.270 kb on + strand at 3237.272 kb on + strand at 3237.272 kb on + strand at 3237.393 kb on + strand at 3237.402 kb on + strand at 3237.516 kb on - strand, within CA265_RS12995 at 3237.602 kb on - strand at 3237.602 kb on - strand at 3237.602 kb on - strand at 3237.602 kb on - strand at 3237.602 kb on - strand at 3237.611 kb on + strand at 3237.612 kb on - strand at 3237.612 kb on - strand at 3237.690 kb on - strand, within CA265_RS13000 at 3237.731 kb on + strand, within CA265_RS13000 at 3237.788 kb on - strand, within CA265_RS13000 at 3237.839 kb on - strand, within CA265_RS13000 at 3237.867 kb on + strand, within CA265_RS13000 at 3237.946 kb on + strand, within CA265_RS13000 at 3237.948 kb on - strand, within CA265_RS13000 at 3237.948 kb on - strand, within CA265_RS13000 at 3237.952 kb on + strand, within CA265_RS13000 at 3238.017 kb on - strand at 3238.038 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.01 mg/ml remove 3,232,893 - +1.5 3,232,911 + -0.2 3,232,919 - +0.3 3,232,919 - +1.0 3,232,928 + +1.5 3,232,983 + +0.6 3,233,091 + CA265_RS12985 0.17 +0.2 3,233,092 - CA265_RS12985 0.17 -0.8 3,233,124 + CA265_RS12985 0.25 -0.2 3,233,125 - CA265_RS12985 0.25 -0.4 3,233,270 + CA265_RS12985 0.60 +0.3 3,233,483 - +1.7 3,233,575 - -1.0 3,233,577 + -0.7 3,233,577 + -1.0 3,233,599 - -0.7 3,233,649 + -1.9 3,233,685 - -2.0 3,233,820 + -1.6 3,233,871 + -0.7 3,233,872 - -1.1 3,233,880 + -1.5 3,233,973 + CA265_RS12990 0.11 -1.0 3,234,044 + CA265_RS12990 0.14 -0.8 3,234,081 - CA265_RS12990 0.15 -0.0 3,234,196 - CA265_RS12990 0.18 -1.3 3,234,211 + CA265_RS12990 0.18 -0.9 3,234,229 + CA265_RS12990 0.19 -0.0 3,234,273 - CA265_RS12990 0.20 -0.0 3,234,288 - CA265_RS12990 0.20 -0.8 3,234,393 + CA265_RS12990 0.23 +0.1 3,234,393 + CA265_RS12990 0.23 -2.5 3,234,571 - CA265_RS12990 0.28 -1.2 3,234,574 + CA265_RS12990 0.28 -1.0 3,234,591 - CA265_RS12990 0.29 -0.7 3,234,749 + CA265_RS12990 0.33 -1.8 3,234,749 + CA265_RS12990 0.33 -2.5 3,234,750 - CA265_RS12990 0.33 -3.1 3,234,750 - CA265_RS12990 0.33 +0.6 3,234,750 - CA265_RS12990 0.33 -0.8 3,234,750 - CA265_RS12990 0.33 -0.7 3,234,848 + CA265_RS12990 0.36 -1.6 3,234,888 + CA265_RS12990 0.37 -1.4 3,234,918 + CA265_RS12990 0.38 -0.8 3,234,981 - CA265_RS12990 0.40 +0.4 3,234,981 - CA265_RS12990 0.40 -0.4 3,235,042 + CA265_RS12990 0.42 +0.5 3,235,044 + CA265_RS12990 0.42 -1.1 3,235,045 - CA265_RS12990 0.42 -0.1 3,235,185 - CA265_RS12990 0.46 +0.1 3,235,329 + CA265_RS12990 0.50 -1.0 3,235,370 + CA265_RS12990 0.51 -1.6 3,235,371 - CA265_RS12990 0.51 -1.0 3,235,371 - CA265_RS12990 0.51 +0.1 3,235,504 + CA265_RS12990 0.55 -0.8 3,235,505 - CA265_RS12990 0.55 -0.8 3,235,626 + CA265_RS12990 0.58 +0.4 3,235,635 + CA265_RS12990 0.58 -0.7 3,235,647 - CA265_RS12990 0.59 -0.7 3,235,667 + CA265_RS12990 0.59 -2.0 3,235,667 + CA265_RS12990 0.59 +0.4 3,235,929 + CA265_RS12990 0.67 -0.5 3,235,929 + CA265_RS12990 0.67 -1.1 3,235,976 - CA265_RS12990 0.68 -0.8 3,235,976 - CA265_RS12990 0.68 -1.3 3,235,976 - CA265_RS12990 0.68 -0.2 3,236,039 + CA265_RS12990 0.70 -1.2 3,236,135 + CA265_RS12990 0.73 -1.3 3,236,135 + CA265_RS12990 0.73 -3.5 3,236,135 + CA265_RS12990 0.73 +0.1 3,236,136 - CA265_RS12990 0.73 -2.1 3,236,176 + CA265_RS12990 0.74 -0.4 3,236,227 + CA265_RS12990 0.75 -1.5 3,236,250 + CA265_RS12990 0.76 -2.0 3,236,251 - CA265_RS12990 0.76 -2.0 3,236,309 + CA265_RS12990 0.77 +0.2 3,236,375 + CA265_RS12990 0.79 -0.6 3,236,508 + CA265_RS12990 0.83 +0.2 3,236,533 - CA265_RS12990 0.84 -1.0 3,236,552 - CA265_RS12990 0.84 -1.2 3,236,584 - CA265_RS12990 0.85 -1.4 3,236,617 - CA265_RS12990 0.86 -0.8 3,236,702 + CA265_RS12990 0.89 +0.1 3,236,703 - CA265_RS12990 0.89 -0.3 3,236,719 - CA265_RS12990 0.89 -0.1 3,236,766 + -0.8 3,236,775 + +0.5 3,236,778 - -0.8 3,236,869 + -1.6 3,236,955 + -1.5 3,237,001 - -0.4 3,237,030 + -0.8 3,237,030 + -0.5 3,237,074 + -2.6 3,237,095 + -0.4 3,237,104 - +0.2 3,237,239 + -1.1 3,237,266 - -1.0 3,237,270 + -1.0 3,237,272 + +0.5 3,237,272 + +0.5 3,237,393 + +0.4 3,237,402 + -0.3 3,237,516 - CA265_RS12995 0.58 -0.1 3,237,602 - -0.9 3,237,602 - -0.7 3,237,602 - -0.0 3,237,602 - -0.3 3,237,602 - +0.3 3,237,611 + +0.3 3,237,612 - +0.9 3,237,612 - +0.3 3,237,690 - CA265_RS13000 0.21 +0.6 3,237,731 + CA265_RS13000 0.31 +0.0 3,237,788 - CA265_RS13000 0.45 +0.2 3,237,839 - CA265_RS13000 0.57 -0.1 3,237,867 + CA265_RS13000 0.64 -0.6 3,237,946 + CA265_RS13000 0.83 -0.1 3,237,948 - CA265_RS13000 0.84 -1.2 3,237,948 - CA265_RS13000 0.84 +1.0 3,237,952 + CA265_RS13000 0.85 +0.0 3,238,017 - -0.3 3,238,038 + +0.3
Or see this region's nucleotide sequence