Experiment: R2A with Cisplatin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS11960 and CA265_RS11965 are separated by 14 nucleotides CA265_RS11965 and CA265_RS11970 are separated by 80 nucleotides
CA265_RS11960: CA265_RS11960 - nucleotidyltransferase, at 2,963,534 to 2,965,030
_RS11960
CA265_RS11965: CA265_RS11965 - error-prone DNA polymerase, at 2,965,045 to 2,968,275
_RS11965
CA265_RS11970: CA265_RS11970 - SAM-dependent methyltransferase, at 2,968,356 to 2,968,979
_RS11970
Position (kb)
2965
2966
2967
2968
2969 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2964.100 kb on + strand, within CA265_RS11960 at 2964.100 kb on + strand, within CA265_RS11960 at 2964.100 kb on + strand, within CA265_RS11960 at 2964.248 kb on + strand, within CA265_RS11960 at 2964.318 kb on + strand, within CA265_RS11960 at 2964.336 kb on + strand, within CA265_RS11960 at 2964.336 kb on + strand, within CA265_RS11960 at 2964.413 kb on - strand, within CA265_RS11960 at 2964.418 kb on + strand, within CA265_RS11960 at 2964.419 kb on - strand, within CA265_RS11960 at 2964.523 kb on + strand, within CA265_RS11960 at 2964.733 kb on + strand, within CA265_RS11960 at 2964.733 kb on + strand, within CA265_RS11960 at 2964.829 kb on - strand, within CA265_RS11960 at 2964.836 kb on + strand, within CA265_RS11960 at 2964.837 kb on - strand, within CA265_RS11960 at 2964.929 kb on + strand at 2964.929 kb on + strand at 2964.930 kb on - strand at 2964.930 kb on - strand at 2964.937 kb on - strand at 2964.983 kb on + strand at 2964.984 kb on - strand at 2965.012 kb on - strand at 2965.012 kb on - strand at 2965.012 kb on - strand at 2965.028 kb on + strand at 2965.037 kb on + strand at 2965.038 kb on - strand at 2965.051 kb on + strand at 2965.069 kb on - strand at 2965.220 kb on + strand at 2965.221 kb on - strand at 2965.363 kb on + strand at 2965.404 kb on + strand, within CA265_RS11965 at 2965.405 kb on - strand, within CA265_RS11965 at 2965.460 kb on - strand, within CA265_RS11965 at 2965.525 kb on + strand, within CA265_RS11965 at 2965.526 kb on - strand, within CA265_RS11965 at 2965.662 kb on - strand, within CA265_RS11965 at 2965.838 kb on - strand, within CA265_RS11965 at 2965.838 kb on - strand, within CA265_RS11965 at 2965.932 kb on - strand, within CA265_RS11965 at 2965.990 kb on + strand, within CA265_RS11965 at 2965.991 kb on - strand, within CA265_RS11965 at 2966.100 kb on - strand, within CA265_RS11965 at 2966.106 kb on + strand, within CA265_RS11965 at 2966.219 kb on + strand, within CA265_RS11965 at 2966.229 kb on + strand, within CA265_RS11965 at 2966.229 kb on + strand, within CA265_RS11965 at 2966.229 kb on + strand, within CA265_RS11965 at 2966.229 kb on + strand, within CA265_RS11965 at 2966.229 kb on + strand, within CA265_RS11965 at 2966.229 kb on + strand, within CA265_RS11965 at 2966.230 kb on - strand, within CA265_RS11965 at 2966.230 kb on - strand, within CA265_RS11965 at 2966.336 kb on + strand, within CA265_RS11965 at 2966.336 kb on + strand, within CA265_RS11965 at 2966.340 kb on + strand, within CA265_RS11965 at 2966.340 kb on + strand, within CA265_RS11965 at 2966.341 kb on - strand, within CA265_RS11965 at 2966.366 kb on + strand, within CA265_RS11965 at 2966.367 kb on - strand, within CA265_RS11965 at 2966.377 kb on - strand, within CA265_RS11965 at 2966.583 kb on + strand, within CA265_RS11965 at 2966.606 kb on + strand, within CA265_RS11965 at 2966.637 kb on - strand, within CA265_RS11965 at 2966.708 kb on + strand, within CA265_RS11965 at 2966.925 kb on + strand, within CA265_RS11965 at 2966.947 kb on + strand, within CA265_RS11965 at 2967.253 kb on + strand, within CA265_RS11965 at 2967.290 kb on + strand, within CA265_RS11965 at 2967.387 kb on + strand, within CA265_RS11965 at 2967.388 kb on - strand, within CA265_RS11965 at 2967.396 kb on + strand, within CA265_RS11965 at 2967.397 kb on - strand, within CA265_RS11965 at 2967.400 kb on + strand, within CA265_RS11965 at 2967.442 kb on + strand, within CA265_RS11965 at 2967.647 kb on + strand, within CA265_RS11965 at 2967.647 kb on + strand, within CA265_RS11965 at 2967.725 kb on + strand, within CA265_RS11965 at 2967.755 kb on + strand, within CA265_RS11965 at 2967.756 kb on - strand, within CA265_RS11965 at 2967.764 kb on - strand, within CA265_RS11965 at 2967.764 kb on - strand, within CA265_RS11965 at 2967.888 kb on - strand, within CA265_RS11965 at 2967.935 kb on + strand, within CA265_RS11965 at 2967.936 kb on - strand, within CA265_RS11965 at 2967.950 kb on + strand, within CA265_RS11965 at 2967.951 kb on - strand at 2967.951 kb on - strand, within CA265_RS11965 at 2967.951 kb on - strand, within CA265_RS11965 at 2967.951 kb on - strand at 2968.034 kb on + strand at 2968.248 kb on + strand at 2968.285 kb on - strand at 2968.348 kb on - strand at 2968.412 kb on + strand at 2968.413 kb on - strand at 2968.459 kb on - strand, within CA265_RS11970 at 2968.682 kb on + strand, within CA265_RS11970 at 2968.683 kb on - strand, within CA265_RS11970 at 2968.891 kb on + strand, within CA265_RS11970 at 2968.891 kb on + strand, within CA265_RS11970 at 2968.892 kb on - strand, within CA265_RS11970 at 2968.892 kb on - strand, within CA265_RS11970 at 2968.925 kb on + strand at 2968.925 kb on + strand at 2968.926 kb on - strand at 2969.042 kb on - strand at 2969.042 kb on - strand at 2969.074 kb on + strand at 2969.190 kb on + strand at 2969.219 kb on + strand at 2969.219 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.01 mg/ml remove 2,964,100 + CA265_RS11960 0.38 +1.2 2,964,100 + CA265_RS11960 0.38 -0.9 2,964,100 + CA265_RS11960 0.38 -0.1 2,964,248 + CA265_RS11960 0.48 +0.6 2,964,318 + CA265_RS11960 0.52 -0.9 2,964,336 + CA265_RS11960 0.54 -0.1 2,964,336 + CA265_RS11960 0.54 -0.3 2,964,413 - CA265_RS11960 0.59 -0.3 2,964,418 + CA265_RS11960 0.59 +0.4 2,964,419 - CA265_RS11960 0.59 +0.4 2,964,523 + CA265_RS11960 0.66 -0.4 2,964,733 + CA265_RS11960 0.80 +0.1 2,964,733 + CA265_RS11960 0.80 -0.8 2,964,829 - CA265_RS11960 0.87 +0.0 2,964,836 + CA265_RS11960 0.87 +0.3 2,964,837 - CA265_RS11960 0.87 +0.2 2,964,929 + -1.4 2,964,929 + +0.1 2,964,930 - -0.6 2,964,930 - +0.6 2,964,937 - -0.4 2,964,983 + +0.5 2,964,984 - -0.5 2,965,012 - +0.0 2,965,012 - -0.1 2,965,012 - +0.8 2,965,028 + +0.3 2,965,037 + +0.1 2,965,038 - +0.4 2,965,051 + +1.0 2,965,069 - -2.0 2,965,220 + +0.3 2,965,221 - -0.0 2,965,363 + +0.4 2,965,404 + CA265_RS11965 0.11 -0.3 2,965,405 - CA265_RS11965 0.11 +0.2 2,965,460 - CA265_RS11965 0.13 +0.1 2,965,525 + CA265_RS11965 0.15 -0.6 2,965,526 - CA265_RS11965 0.15 +0.2 2,965,662 - CA265_RS11965 0.19 -0.5 2,965,838 - CA265_RS11965 0.25 -0.2 2,965,838 - CA265_RS11965 0.25 -0.3 2,965,932 - CA265_RS11965 0.27 -3.0 2,965,990 + CA265_RS11965 0.29 -0.9 2,965,991 - CA265_RS11965 0.29 +1.7 2,966,100 - CA265_RS11965 0.33 -0.9 2,966,106 + CA265_RS11965 0.33 -0.9 2,966,219 + CA265_RS11965 0.36 -0.3 2,966,229 + CA265_RS11965 0.37 -0.5 2,966,229 + CA265_RS11965 0.37 -1.4 2,966,229 + CA265_RS11965 0.37 -0.6 2,966,229 + CA265_RS11965 0.37 -0.9 2,966,229 + CA265_RS11965 0.37 +0.3 2,966,229 + CA265_RS11965 0.37 -1.0 2,966,230 - CA265_RS11965 0.37 -0.4 2,966,230 - CA265_RS11965 0.37 -0.1 2,966,336 + CA265_RS11965 0.40 -0.6 2,966,336 + CA265_RS11965 0.40 +0.1 2,966,340 + CA265_RS11965 0.40 +0.0 2,966,340 + CA265_RS11965 0.40 +0.6 2,966,341 - CA265_RS11965 0.40 -0.7 2,966,366 + CA265_RS11965 0.41 -0.2 2,966,367 - CA265_RS11965 0.41 +1.3 2,966,377 - CA265_RS11965 0.41 -0.8 2,966,583 + CA265_RS11965 0.48 -0.1 2,966,606 + CA265_RS11965 0.48 +0.1 2,966,637 - CA265_RS11965 0.49 -0.2 2,966,708 + CA265_RS11965 0.51 -0.9 2,966,925 + CA265_RS11965 0.58 -0.4 2,966,947 + CA265_RS11965 0.59 -0.1 2,967,253 + CA265_RS11965 0.68 -0.8 2,967,290 + CA265_RS11965 0.69 -1.0 2,967,387 + CA265_RS11965 0.72 -0.0 2,967,388 - CA265_RS11965 0.73 -0.2 2,967,396 + CA265_RS11965 0.73 -0.4 2,967,397 - CA265_RS11965 0.73 -0.2 2,967,400 + CA265_RS11965 0.73 -0.3 2,967,442 + CA265_RS11965 0.74 +0.4 2,967,647 + CA265_RS11965 0.81 +0.8 2,967,647 + CA265_RS11965 0.81 +1.6 2,967,725 + CA265_RS11965 0.83 +0.1 2,967,755 + CA265_RS11965 0.84 -0.3 2,967,756 - CA265_RS11965 0.84 +0.9 2,967,764 - CA265_RS11965 0.84 -0.9 2,967,764 - CA265_RS11965 0.84 +1.3 2,967,888 - CA265_RS11965 0.88 +0.4 2,967,935 + CA265_RS11965 0.89 +0.8 2,967,936 - CA265_RS11965 0.89 -1.9 2,967,950 + CA265_RS11965 0.90 +0.2 2,967,951 - +0.8 2,967,951 - CA265_RS11965 0.90 +0.4 2,967,951 - CA265_RS11965 0.90 +1.2 2,967,951 - -0.2 2,968,034 + +0.1 2,968,248 + +1.9 2,968,285 - -2.0 2,968,348 - +0.9 2,968,412 + -0.2 2,968,413 - -0.4 2,968,459 - CA265_RS11970 0.17 -1.2 2,968,682 + CA265_RS11970 0.52 +0.7 2,968,683 - CA265_RS11970 0.52 -0.3 2,968,891 + CA265_RS11970 0.86 -0.4 2,968,891 + CA265_RS11970 0.86 -0.1 2,968,892 - CA265_RS11970 0.86 +0.1 2,968,892 - CA265_RS11970 0.86 -0.0 2,968,925 + +0.8 2,968,925 + -1.8 2,968,926 - +0.9 2,969,042 - +1.4 2,969,042 - +0.1 2,969,074 + +1.5 2,969,190 + -1.2 2,969,219 + +0.4 2,969,219 + +0.2
Or see this region's nucleotide sequence