Experiment: R2A with Cisplatin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS11035 and CA265_RS11040 are separated by 224 nucleotides CA265_RS11040 and CA265_RS11045 are separated by 7 nucleotides
CA265_RS11035: CA265_RS11035 - phosphohydrolase, at 2,709,348 to 2,710,202
_RS11035
CA265_RS11040: CA265_RS11040 - hypothetical protein, at 2,710,427 to 2,711,080
_RS11040
CA265_RS11045: CA265_RS11045 - ABC transporter, at 2,711,088 to 2,712,386
_RS11045
Position (kb)
2710
2711
2712 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2709.464 kb on - strand, within CA265_RS11035 at 2709.522 kb on + strand, within CA265_RS11035 at 2709.580 kb on - strand, within CA265_RS11035 at 2709.584 kb on + strand, within CA265_RS11035 at 2709.597 kb on - strand, within CA265_RS11035 at 2709.602 kb on + strand, within CA265_RS11035 at 2709.602 kb on + strand, within CA265_RS11035 at 2709.602 kb on + strand, within CA265_RS11035 at 2709.603 kb on - strand, within CA265_RS11035 at 2709.603 kb on - strand, within CA265_RS11035 at 2709.839 kb on - strand, within CA265_RS11035 at 2709.856 kb on - strand, within CA265_RS11035 at 2709.856 kb on - strand, within CA265_RS11035 at 2709.908 kb on - strand, within CA265_RS11035 at 2709.921 kb on - strand, within CA265_RS11035 at 2709.968 kb on + strand, within CA265_RS11035 at 2710.011 kb on + strand, within CA265_RS11035 at 2710.011 kb on + strand, within CA265_RS11035 at 2710.134 kb on - strand at 2710.211 kb on - strand at 2710.228 kb on - strand at 2710.284 kb on - strand at 2710.304 kb on + strand at 2710.348 kb on + strand at 2710.348 kb on + strand at 2710.407 kb on + strand at 2710.413 kb on + strand at 2710.424 kb on + strand at 2710.426 kb on + strand at 2710.427 kb on - strand at 2710.461 kb on + strand at 2710.462 kb on - strand at 2710.462 kb on - strand at 2710.504 kb on + strand, within CA265_RS11040 at 2710.573 kb on - strand, within CA265_RS11040 at 2710.573 kb on - strand, within CA265_RS11040 at 2710.647 kb on - strand, within CA265_RS11040 at 2710.649 kb on - strand, within CA265_RS11040 at 2710.679 kb on + strand, within CA265_RS11040 at 2710.680 kb on - strand, within CA265_RS11040 at 2710.680 kb on - strand, within CA265_RS11040 at 2710.685 kb on + strand, within CA265_RS11040 at 2710.724 kb on + strand, within CA265_RS11040 at 2710.725 kb on - strand, within CA265_RS11040 at 2710.756 kb on + strand, within CA265_RS11040 at 2710.813 kb on + strand, within CA265_RS11040 at 2710.827 kb on + strand, within CA265_RS11040 at 2710.832 kb on - strand, within CA265_RS11040 at 2710.865 kb on - strand, within CA265_RS11040 at 2711.014 kb on + strand, within CA265_RS11040 at 2711.079 kb on - strand at 2711.114 kb on + strand at 2711.120 kb on + strand at 2711.172 kb on + strand at 2711.175 kb on - strand at 2711.175 kb on - strand at 2711.178 kb on - strand at 2711.228 kb on - strand, within CA265_RS11045 at 2711.255 kb on + strand, within CA265_RS11045 at 2711.321 kb on + strand, within CA265_RS11045 at 2711.337 kb on + strand, within CA265_RS11045 at 2711.341 kb on + strand, within CA265_RS11045 at 2711.582 kb on - strand, within CA265_RS11045 at 2711.631 kb on - strand, within CA265_RS11045 at 2711.634 kb on + strand, within CA265_RS11045 at 2711.635 kb on - strand, within CA265_RS11045 at 2711.676 kb on + strand, within CA265_RS11045 at 2711.751 kb on + strand, within CA265_RS11045 at 2711.752 kb on - strand, within CA265_RS11045 at 2711.752 kb on - strand, within CA265_RS11045 at 2711.807 kb on - strand, within CA265_RS11045 at 2711.914 kb on + strand, within CA265_RS11045 at 2711.991 kb on - strand, within CA265_RS11045 at 2712.047 kb on - strand, within CA265_RS11045 at 2712.071 kb on - strand, within CA265_RS11045
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.01 mg/ml remove 2,709,464 - CA265_RS11035 0.14 -1.0 2,709,522 + CA265_RS11035 0.20 -0.2 2,709,580 - CA265_RS11035 0.27 +0.6 2,709,584 + CA265_RS11035 0.28 -1.4 2,709,597 - CA265_RS11035 0.29 -1.2 2,709,602 + CA265_RS11035 0.30 +0.4 2,709,602 + CA265_RS11035 0.30 -1.0 2,709,602 + CA265_RS11035 0.30 -2.5 2,709,603 - CA265_RS11035 0.30 -0.7 2,709,603 - CA265_RS11035 0.30 -1.3 2,709,839 - CA265_RS11035 0.57 -0.8 2,709,856 - CA265_RS11035 0.59 -0.2 2,709,856 - CA265_RS11035 0.59 +2.1 2,709,908 - CA265_RS11035 0.65 -0.8 2,709,921 - CA265_RS11035 0.67 +1.4 2,709,968 + CA265_RS11035 0.73 -0.1 2,710,011 + CA265_RS11035 0.78 +1.0 2,710,011 + CA265_RS11035 0.78 +0.1 2,710,134 - -0.1 2,710,211 - +1.1 2,710,228 - +0.0 2,710,284 - -0.3 2,710,304 + +0.5 2,710,348 + -0.4 2,710,348 + -0.5 2,710,407 + +0.8 2,710,413 + +0.2 2,710,424 + -0.4 2,710,426 + -0.2 2,710,427 - +0.1 2,710,461 + -0.2 2,710,462 - +0.2 2,710,462 - -0.1 2,710,504 + CA265_RS11040 0.12 -0.2 2,710,573 - CA265_RS11040 0.22 +0.3 2,710,573 - CA265_RS11040 0.22 -1.5 2,710,647 - CA265_RS11040 0.34 +0.2 2,710,649 - CA265_RS11040 0.34 -0.3 2,710,679 + CA265_RS11040 0.39 +0.2 2,710,680 - CA265_RS11040 0.39 -1.4 2,710,680 - CA265_RS11040 0.39 +0.9 2,710,685 + CA265_RS11040 0.39 -0.2 2,710,724 + CA265_RS11040 0.45 +0.5 2,710,725 - CA265_RS11040 0.46 -0.5 2,710,756 + CA265_RS11040 0.50 +0.2 2,710,813 + CA265_RS11040 0.59 -1.1 2,710,827 + CA265_RS11040 0.61 +0.9 2,710,832 - CA265_RS11040 0.62 -1.1 2,710,865 - CA265_RS11040 0.67 +1.5 2,711,014 + CA265_RS11040 0.90 -0.5 2,711,079 - -0.8 2,711,114 + -0.0 2,711,120 + -0.4 2,711,172 + -0.2 2,711,175 - +0.4 2,711,175 - +0.0 2,711,178 - -1.8 2,711,228 - CA265_RS11045 0.11 +0.1 2,711,255 + CA265_RS11045 0.13 -0.2 2,711,321 + CA265_RS11045 0.18 -0.3 2,711,337 + CA265_RS11045 0.19 +0.9 2,711,341 + CA265_RS11045 0.19 +0.1 2,711,582 - CA265_RS11045 0.38 -0.3 2,711,631 - CA265_RS11045 0.42 +0.5 2,711,634 + CA265_RS11045 0.42 -1.6 2,711,635 - CA265_RS11045 0.42 +0.9 2,711,676 + CA265_RS11045 0.45 -0.4 2,711,751 + CA265_RS11045 0.51 +1.1 2,711,752 - CA265_RS11045 0.51 -0.6 2,711,752 - CA265_RS11045 0.51 -0.8 2,711,807 - CA265_RS11045 0.55 -0.6 2,711,914 + CA265_RS11045 0.64 -0.1 2,711,991 - CA265_RS11045 0.70 +0.4 2,712,047 - CA265_RS11045 0.74 +0.9 2,712,071 - CA265_RS11045 0.76 +0.5
Or see this region's nucleotide sequence