Experiment: R2A with Chlorite 4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27825 and RR42_RS27830 overlap by 4 nucleotides RR42_RS27830 and RR42_RS27835 are separated by 59 nucleotides RR42_RS27835 and RR42_RS27840 are separated by 37 nucleotides RR42_RS27840 and RR42_RS27845 are separated by 65 nucleotides
RR42_RS27825: RR42_RS27825 - 4-hydroxyphenylacetate isomerase, at 1,607,156 to 1,607,887
_RS27825
RR42_RS27830: RR42_RS27830 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 1,607,884 to 1,608,648
_RS27830
RR42_RS27835: RR42_RS27835 - betaine-aldehyde dehydrogenase, at 1,608,708 to 1,610,168
_RS27835
RR42_RS27840: RR42_RS27840 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 1,610,206 to 1,611,054
_RS27840
RR42_RS27845: RR42_RS27845 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 1,611,120 to 1,611,509
_RS27845
Position (kb) 1608
1609
1610
1611 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1607.838 kb on + strand at 1607.838 kb on + strand at 1607.948 kb on + strand at 1607.948 kb on + strand at 1608.043 kb on + strand, within RR42_RS27830 at 1608.043 kb on + strand, within RR42_RS27830 at 1608.044 kb on - strand, within RR42_RS27830 at 1608.138 kb on + strand, within RR42_RS27830 at 1608.138 kb on + strand, within RR42_RS27830 at 1608.139 kb on - strand, within RR42_RS27830 at 1608.139 kb on - strand, within RR42_RS27830 at 1608.139 kb on - strand, within RR42_RS27830 at 1608.139 kb on - strand, within RR42_RS27830 at 1608.139 kb on - strand, within RR42_RS27830 at 1608.163 kb on + strand, within RR42_RS27830 at 1608.164 kb on - strand, within RR42_RS27830 at 1608.172 kb on + strand, within RR42_RS27830 at 1608.173 kb on - strand, within RR42_RS27830 at 1608.235 kb on + strand, within RR42_RS27830 at 1608.235 kb on + strand, within RR42_RS27830 at 1608.241 kb on + strand, within RR42_RS27830 at 1608.241 kb on + strand, within RR42_RS27830 at 1608.242 kb on - strand, within RR42_RS27830 at 1608.272 kb on - strand, within RR42_RS27830 at 1608.298 kb on + strand, within RR42_RS27830 at 1608.298 kb on + strand, within RR42_RS27830 at 1608.481 kb on + strand, within RR42_RS27830 at 1608.482 kb on - strand, within RR42_RS27830 at 1608.872 kb on + strand, within RR42_RS27835 at 1608.872 kb on + strand, within RR42_RS27835 at 1609.095 kb on + strand, within RR42_RS27835 at 1609.096 kb on - strand, within RR42_RS27835 at 1609.096 kb on - strand, within RR42_RS27835 at 1609.695 kb on + strand, within RR42_RS27835 at 1609.695 kb on + strand, within RR42_RS27835 at 1609.696 kb on - strand, within RR42_RS27835 at 1609.696 kb on - strand, within RR42_RS27835 at 1609.923 kb on + strand, within RR42_RS27835 at 1609.924 kb on - strand, within RR42_RS27835 at 1609.924 kb on - strand, within RR42_RS27835 at 1609.924 kb on - strand, within RR42_RS27835 at 1609.938 kb on + strand, within RR42_RS27835 at 1609.939 kb on - strand, within RR42_RS27835 at 1610.147 kb on + strand at 1610.148 kb on - strand at 1610.216 kb on + strand at 1610.216 kb on + strand at 1610.431 kb on + strand, within RR42_RS27840 at 1610.431 kb on + strand, within RR42_RS27840 at 1610.431 kb on + strand, within RR42_RS27840 at 1610.431 kb on + strand, within RR42_RS27840 at 1610.474 kb on - strand, within RR42_RS27840 at 1610.603 kb on - strand, within RR42_RS27840 at 1610.897 kb on - strand, within RR42_RS27840 at 1610.972 kb on - strand at 1610.973 kb on - strand at 1611.142 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chlorite 4 mM remove 1,607,838 + +0.3 1,607,838 + +0.5 1,607,948 + -0.1 1,607,948 + +0.6 1,608,043 + RR42_RS27830 0.21 -0.6 1,608,043 + RR42_RS27830 0.21 +0.7 1,608,044 - RR42_RS27830 0.21 +0.3 1,608,138 + RR42_RS27830 0.33 -1.5 1,608,138 + RR42_RS27830 0.33 +0.2 1,608,139 - RR42_RS27830 0.33 +0.1 1,608,139 - RR42_RS27830 0.33 -0.0 1,608,139 - RR42_RS27830 0.33 -0.1 1,608,139 - RR42_RS27830 0.33 -1.3 1,608,139 - RR42_RS27830 0.33 -0.9 1,608,163 + RR42_RS27830 0.36 -0.7 1,608,164 - RR42_RS27830 0.37 +1.6 1,608,172 + RR42_RS27830 0.38 -0.8 1,608,173 - RR42_RS27830 0.38 -0.3 1,608,235 + RR42_RS27830 0.46 +0.4 1,608,235 + RR42_RS27830 0.46 +0.0 1,608,241 + RR42_RS27830 0.47 -0.7 1,608,241 + RR42_RS27830 0.47 +0.3 1,608,242 - RR42_RS27830 0.47 +0.1 1,608,272 - RR42_RS27830 0.51 +2.0 1,608,298 + RR42_RS27830 0.54 +1.0 1,608,298 + RR42_RS27830 0.54 +0.2 1,608,481 + RR42_RS27830 0.78 +0.1 1,608,482 - RR42_RS27830 0.78 -0.7 1,608,872 + RR42_RS27835 0.11 -0.3 1,608,872 + RR42_RS27835 0.11 +0.4 1,609,095 + RR42_RS27835 0.26 +1.4 1,609,096 - RR42_RS27835 0.27 +0.2 1,609,096 - RR42_RS27835 0.27 +1.2 1,609,695 + RR42_RS27835 0.68 +0.6 1,609,695 + RR42_RS27835 0.68 +0.2 1,609,696 - RR42_RS27835 0.68 +0.5 1,609,696 - RR42_RS27835 0.68 -1.9 1,609,923 + RR42_RS27835 0.83 -0.0 1,609,924 - RR42_RS27835 0.83 -0.8 1,609,924 - RR42_RS27835 0.83 +0.3 1,609,924 - RR42_RS27835 0.83 +0.2 1,609,938 + RR42_RS27835 0.84 +0.2 1,609,939 - RR42_RS27835 0.84 -0.3 1,610,147 + -0.6 1,610,148 - +0.8 1,610,216 + -0.1 1,610,216 + -0.7 1,610,431 + RR42_RS27840 0.27 +0.4 1,610,431 + RR42_RS27840 0.27 -0.0 1,610,431 + RR42_RS27840 0.27 +0.0 1,610,431 + RR42_RS27840 0.27 -0.1 1,610,474 - RR42_RS27840 0.32 +1.9 1,610,603 - RR42_RS27840 0.47 -2.0 1,610,897 - RR42_RS27840 0.81 +0.8 1,610,972 - -0.6 1,610,973 - -1.6 1,611,142 - +2.7
Or see this region's nucleotide sequence