Experiment: R2A with Chlorite 4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21570 and RR42_RS21575 are separated by 125 nucleotides RR42_RS21575 and RR42_RS21580 are separated by 34 nucleotides
RR42_RS21570: RR42_RS21570 - membrane protein, at 164,956 to 166,104
_RS21570
RR42_RS21575: RR42_RS21575 - peptidase E, at 166,230 to 166,985
_RS21575
RR42_RS21580: RR42_RS21580 - cysteine desulfurase, at 167,020 to 168,204
_RS21580
Position (kb)
166
167 Strain fitness (log2 ratio)
-2
-1
0
1 at 165.236 kb on + strand, within RR42_RS21570 at 165.237 kb on - strand, within RR42_RS21570 at 165.237 kb on - strand, within RR42_RS21570 at 165.237 kb on - strand, within RR42_RS21570 at 165.237 kb on - strand, within RR42_RS21570 at 165.237 kb on - strand, within RR42_RS21570 at 165.400 kb on - strand, within RR42_RS21570 at 165.400 kb on - strand, within RR42_RS21570 at 165.401 kb on + strand, within RR42_RS21570 at 165.401 kb on + strand, within RR42_RS21570 at 165.402 kb on - strand, within RR42_RS21570 at 165.402 kb on - strand, within RR42_RS21570 at 165.402 kb on - strand, within RR42_RS21570 at 165.402 kb on - strand, within RR42_RS21570 at 165.452 kb on + strand, within RR42_RS21570 at 165.527 kb on + strand, within RR42_RS21570 at 165.527 kb on + strand, within RR42_RS21570 at 165.617 kb on + strand, within RR42_RS21570 at 165.618 kb on - strand, within RR42_RS21570 at 165.630 kb on - strand, within RR42_RS21570 at 165.648 kb on - strand, within RR42_RS21570 at 165.716 kb on + strand, within RR42_RS21570 at 165.717 kb on - strand, within RR42_RS21570 at 165.717 kb on - strand, within RR42_RS21570 at 165.717 kb on - strand, within RR42_RS21570 at 165.717 kb on - strand, within RR42_RS21570 at 165.717 kb on - strand, within RR42_RS21570 at 165.719 kb on + strand, within RR42_RS21570 at 165.719 kb on + strand, within RR42_RS21570 at 165.720 kb on - strand, within RR42_RS21570 at 165.744 kb on - strand, within RR42_RS21570 at 165.744 kb on - strand, within RR42_RS21570 at 165.795 kb on - strand, within RR42_RS21570 at 165.795 kb on - strand, within RR42_RS21570 at 165.795 kb on - strand, within RR42_RS21570 at 165.795 kb on - strand, within RR42_RS21570 at 165.961 kb on - strand, within RR42_RS21570 at 165.999 kb on - strand at 166.128 kb on - strand at 166.128 kb on - strand at 166.128 kb on - strand at 166.318 kb on - strand, within RR42_RS21575 at 166.631 kb on - strand, within RR42_RS21575 at 166.831 kb on + strand, within RR42_RS21575 at 166.831 kb on + strand, within RR42_RS21575 at 166.831 kb on + strand, within RR42_RS21575 at 166.831 kb on + strand, within RR42_RS21575 at 166.831 kb on + strand, within RR42_RS21575 at 166.831 kb on + strand, within RR42_RS21575 at 166.831 kb on + strand, within RR42_RS21575 at 166.832 kb on - strand, within RR42_RS21575 at 166.832 kb on - strand, within RR42_RS21575 at 166.832 kb on - strand, within RR42_RS21575 at 166.832 kb on - strand, within RR42_RS21575 at 166.832 kb on - strand, within RR42_RS21575 at 166.934 kb on - strand at 166.998 kb on + strand at 167.073 kb on + strand at 167.074 kb on - strand at 167.074 kb on - strand at 167.081 kb on + strand at 167.082 kb on - strand at 167.117 kb on + strand at 167.264 kb on + strand, within RR42_RS21580 at 167.468 kb on + strand, within RR42_RS21580 at 167.468 kb on + strand, within RR42_RS21580 at 167.469 kb on - strand, within RR42_RS21580 at 167.469 kb on - strand, within RR42_RS21580 at 167.531 kb on + strand, within RR42_RS21580 at 167.943 kb on + strand, within RR42_RS21580 at 167.943 kb on + strand, within RR42_RS21580 at 167.943 kb on + strand, within RR42_RS21580 at 167.944 kb on - strand, within RR42_RS21580 at 167.944 kb on - strand, within RR42_RS21580
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chlorite 4 mM remove 165,236 + RR42_RS21570 0.24 -0.5 165,237 - RR42_RS21570 0.24 +0.3 165,237 - RR42_RS21570 0.24 +0.1 165,237 - RR42_RS21570 0.24 -0.4 165,237 - RR42_RS21570 0.24 +0.6 165,237 - RR42_RS21570 0.24 +0.5 165,400 - RR42_RS21570 0.39 +0.3 165,400 - RR42_RS21570 0.39 +0.2 165,401 + RR42_RS21570 0.39 -0.7 165,401 + RR42_RS21570 0.39 +0.0 165,402 - RR42_RS21570 0.39 -0.0 165,402 - RR42_RS21570 0.39 +0.2 165,402 - RR42_RS21570 0.39 +0.2 165,402 - RR42_RS21570 0.39 -1.6 165,452 + RR42_RS21570 0.43 +1.7 165,527 + RR42_RS21570 0.50 +0.4 165,527 + RR42_RS21570 0.50 -0.9 165,617 + RR42_RS21570 0.58 -0.1 165,618 - RR42_RS21570 0.58 -0.0 165,630 - RR42_RS21570 0.59 +0.3 165,648 - RR42_RS21570 0.60 -0.2 165,716 + RR42_RS21570 0.66 +0.4 165,717 - RR42_RS21570 0.66 +1.0 165,717 - RR42_RS21570 0.66 +0.9 165,717 - RR42_RS21570 0.66 +1.0 165,717 - RR42_RS21570 0.66 +0.0 165,717 - RR42_RS21570 0.66 -0.4 165,719 + RR42_RS21570 0.66 +0.0 165,719 + RR42_RS21570 0.66 -0.4 165,720 - RR42_RS21570 0.66 +0.7 165,744 - RR42_RS21570 0.69 -1.4 165,744 - RR42_RS21570 0.69 -0.1 165,795 - RR42_RS21570 0.73 +0.6 165,795 - RR42_RS21570 0.73 +0.5 165,795 - RR42_RS21570 0.73 +0.8 165,795 - RR42_RS21570 0.73 -0.1 165,961 - RR42_RS21570 0.87 +0.2 165,999 - -0.4 166,128 - +0.4 166,128 - -0.1 166,128 - -0.4 166,318 - RR42_RS21575 0.12 -0.3 166,631 - RR42_RS21575 0.53 -0.2 166,831 + RR42_RS21575 0.79 +0.2 166,831 + RR42_RS21575 0.79 +0.7 166,831 + RR42_RS21575 0.79 +0.7 166,831 + RR42_RS21575 0.79 -0.7 166,831 + RR42_RS21575 0.79 +0.7 166,831 + RR42_RS21575 0.79 +0.2 166,831 + RR42_RS21575 0.79 -0.5 166,832 - RR42_RS21575 0.80 +0.4 166,832 - RR42_RS21575 0.80 -0.2 166,832 - RR42_RS21575 0.80 +1.2 166,832 - RR42_RS21575 0.80 -1.8 166,832 - RR42_RS21575 0.80 +0.3 166,934 - -1.0 166,998 + -1.8 167,073 + -0.7 167,074 - -2.4 167,074 - +0.4 167,081 + +0.2 167,082 - +1.2 167,117 + -0.1 167,264 + RR42_RS21580 0.21 +0.4 167,468 + RR42_RS21580 0.38 -0.3 167,468 + RR42_RS21580 0.38 +0.8 167,469 - RR42_RS21580 0.38 +0.7 167,469 - RR42_RS21580 0.38 -1.1 167,531 + RR42_RS21580 0.43 -0.2 167,943 + RR42_RS21580 0.78 -1.2 167,943 + RR42_RS21580 0.78 -0.1 167,943 + RR42_RS21580 0.78 +0.6 167,944 - RR42_RS21580 0.78 -0.1 167,944 - RR42_RS21580 0.78 +1.2
Or see this region's nucleotide sequence