Experiment: R2A with Chlorite 4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21545 and RR42_RS21550 are separated by 197 nucleotides RR42_RS21550 and RR42_RS21555 are separated by 259 nucleotides RR42_RS21555 and RR42_RS21560 are separated by 11 nucleotides RR42_RS21560 and RR42_RS21565 are separated by 120 nucleotides
RR42_RS21545: RR42_RS21545 - hypothetical protein, at 160,117 to 160,659
_RS21545
RR42_RS21550: RR42_RS21550 - hypothetical protein, at 160,857 to 161,270
_RS21550
RR42_RS21555: RR42_RS21555 - membrane protein, at 161,530 to 162,519
_RS21555
RR42_RS21560: RR42_RS21560 - hypothetical protein, at 162,531 to 163,229
_RS21560
RR42_RS21565: RR42_RS21565 - hypothetical protein, at 163,350 to 164,309
_RS21565
Position (kb) 161
162
163 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 160.571 kb on + strand, within RR42_RS21545 at 160.711 kb on + strand at 160.712 kb on - strand at 160.755 kb on + strand at 160.755 kb on + strand at 160.756 kb on - strand at 160.765 kb on - strand at 160.952 kb on + strand, within RR42_RS21550 at 160.980 kb on + strand, within RR42_RS21550 at 160.981 kb on - strand, within RR42_RS21550 at 160.981 kb on - strand, within RR42_RS21550 at 161.011 kb on - strand, within RR42_RS21550 at 161.011 kb on - strand, within RR42_RS21550 at 161.011 kb on - strand, within RR42_RS21550 at 161.012 kb on + strand, within RR42_RS21550 at 161.013 kb on - strand, within RR42_RS21550 at 161.013 kb on - strand, within RR42_RS21550 at 161.013 kb on - strand, within RR42_RS21550 at 161.409 kb on + strand at 161.445 kb on - strand at 161.445 kb on - strand at 161.452 kb on - strand at 161.452 kb on - strand at 161.452 kb on - strand at 161.452 kb on - strand at 161.452 kb on - strand at 161.489 kb on - strand at 161.513 kb on + strand at 161.514 kb on - strand at 161.514 kb on - strand at 161.734 kb on + strand, within RR42_RS21555 at 161.735 kb on - strand, within RR42_RS21555 at 161.831 kb on - strand, within RR42_RS21555 at 161.831 kb on - strand, within RR42_RS21555 at 161.944 kb on + strand, within RR42_RS21555 at 161.944 kb on + strand, within RR42_RS21555 at 162.043 kb on + strand, within RR42_RS21555 at 162.043 kb on + strand, within RR42_RS21555 at 162.043 kb on + strand, within RR42_RS21555 at 162.044 kb on - strand, within RR42_RS21555 at 162.044 kb on - strand, within RR42_RS21555 at 162.044 kb on - strand, within RR42_RS21555 at 162.044 kb on - strand, within RR42_RS21555 at 162.044 kb on - strand, within RR42_RS21555 at 162.278 kb on + strand, within RR42_RS21555 at 162.316 kb on + strand, within RR42_RS21555 at 162.338 kb on + strand, within RR42_RS21555 at 162.387 kb on + strand, within RR42_RS21555 at 162.548 kb on - strand at 162.580 kb on + strand at 162.580 kb on + strand at 162.580 kb on + strand at 162.580 kb on + strand at 162.580 kb on + strand at 162.581 kb on - strand at 162.581 kb on - strand at 162.581 kb on - strand at 162.581 kb on - strand at 162.581 kb on - strand at 162.616 kb on + strand, within RR42_RS21560 at 162.617 kb on - strand, within RR42_RS21560 at 162.678 kb on + strand, within RR42_RS21560 at 162.761 kb on + strand, within RR42_RS21560 at 162.871 kb on + strand, within RR42_RS21560 at 162.872 kb on - strand, within RR42_RS21560 at 163.240 kb on + strand at 163.278 kb on + strand at 163.278 kb on + strand at 163.278 kb on + strand at 163.279 kb on - strand at 163.279 kb on - strand at 163.279 kb on - strand at 163.279 kb on - strand at 163.393 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chlorite 4 mM remove 160,571 + RR42_RS21545 0.84 -1.1 160,711 + -0.9 160,712 - +0.6 160,755 + -0.8 160,755 + -1.1 160,756 - +0.6 160,765 - -3.0 160,952 + RR42_RS21550 0.23 -0.3 160,980 + RR42_RS21550 0.30 -0.1 160,981 - RR42_RS21550 0.30 -0.7 160,981 - RR42_RS21550 0.30 -0.2 161,011 - RR42_RS21550 0.37 +1.8 161,011 - RR42_RS21550 0.37 -0.6 161,011 - RR42_RS21550 0.37 -0.1 161,012 + RR42_RS21550 0.37 -1.6 161,013 - RR42_RS21550 0.38 -0.8 161,013 - RR42_RS21550 0.38 -0.7 161,013 - RR42_RS21550 0.38 +0.1 161,409 + -1.6 161,445 - -0.5 161,445 - -0.0 161,452 - +0.6 161,452 - +0.5 161,452 - +0.4 161,452 - +0.9 161,452 - -0.4 161,489 - -0.4 161,513 + -1.2 161,514 - +1.1 161,514 - +0.9 161,734 + RR42_RS21555 0.21 -0.9 161,735 - RR42_RS21555 0.21 +0.3 161,831 - RR42_RS21555 0.30 +0.9 161,831 - RR42_RS21555 0.30 -0.7 161,944 + RR42_RS21555 0.42 +0.6 161,944 + RR42_RS21555 0.42 +0.7 162,043 + RR42_RS21555 0.52 +1.0 162,043 + RR42_RS21555 0.52 -0.7 162,043 + RR42_RS21555 0.52 +0.4 162,044 - RR42_RS21555 0.52 -2.6 162,044 - RR42_RS21555 0.52 +0.2 162,044 - RR42_RS21555 0.52 +0.4 162,044 - RR42_RS21555 0.52 +0.2 162,044 - RR42_RS21555 0.52 +0.8 162,278 + RR42_RS21555 0.76 -0.4 162,316 + RR42_RS21555 0.79 +0.1 162,338 + RR42_RS21555 0.82 -1.4 162,387 + RR42_RS21555 0.87 -0.2 162,548 - -0.2 162,580 + -0.2 162,580 + -1.0 162,580 + -0.4 162,580 + +1.2 162,580 + -2.1 162,581 - +1.5 162,581 - -2.4 162,581 - -0.1 162,581 - -0.0 162,581 - +0.2 162,616 + RR42_RS21560 0.12 -1.3 162,617 - RR42_RS21560 0.12 -0.6 162,678 + RR42_RS21560 0.21 -0.5 162,761 + RR42_RS21560 0.33 -0.2 162,871 + RR42_RS21560 0.49 +0.2 162,872 - RR42_RS21560 0.49 -1.0 163,240 + -0.9 163,278 + -1.5 163,278 + +1.2 163,278 + -2.1 163,279 - +0.1 163,279 - +0.1 163,279 - -0.7 163,279 - +0.5 163,393 + -0.2
Or see this region's nucleotide sequence