Experiment: R2A with Chlorite 4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14980 and RR42_RS14985 are separated by 130 nucleotides RR42_RS14985 and RR42_RS14990 are separated by 111 nucleotides RR42_RS14990 and RR42_RS14995 are separated by 184 nucleotides
RR42_RS14980: RR42_RS14980 - glutamyl-tRNA amidotransferase, at 3,239,567 to 3,240,016
_RS14980
RR42_RS14985: RR42_RS14985 - 30S ribosomal protein S21, at 3,240,147 to 3,240,359
_RS14985
RR42_RS14990: RR42_RS14990 - membrane protein, at 3,240,471 to 3,241,745
_RS14990
RR42_RS14995: RR42_RS14995 - tRNA threonylcarbamoyladenosine modification protein TsaD, at 3,241,930 to 3,242,970
_RS14995
Position (kb)
3240
3241
3242 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3239.513 kb on + strand at 3239.551 kb on + strand at 3239.660 kb on - strand, within RR42_RS14980 at 3239.751 kb on + strand, within RR42_RS14980 at 3239.751 kb on + strand, within RR42_RS14980 at 3239.751 kb on + strand, within RR42_RS14980 at 3239.752 kb on - strand, within RR42_RS14980 at 3240.022 kb on + strand at 3240.152 kb on - strand at 3240.152 kb on - strand at 3240.191 kb on - strand, within RR42_RS14985 at 3240.371 kb on - strand at 3240.403 kb on - strand at 3240.403 kb on - strand at 3240.410 kb on + strand at 3240.433 kb on + strand at 3240.557 kb on + strand at 3240.557 kb on + strand at 3240.558 kb on - strand at 3240.559 kb on + strand at 3240.560 kb on - strand at 3240.604 kb on + strand, within RR42_RS14990 at 3240.604 kb on + strand, within RR42_RS14990 at 3240.767 kb on + strand, within RR42_RS14990 at 3240.767 kb on + strand, within RR42_RS14990 at 3241.471 kb on + strand, within RR42_RS14990 at 3241.471 kb on + strand, within RR42_RS14990 at 3241.774 kb on - strand at 3241.830 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chlorite 4 mM remove 3,239,513 + +0.2 3,239,551 + -0.2 3,239,660 - RR42_RS14980 0.21 +0.5 3,239,751 + RR42_RS14980 0.41 -1.6 3,239,751 + RR42_RS14980 0.41 -1.2 3,239,751 + RR42_RS14980 0.41 -1.9 3,239,752 - RR42_RS14980 0.41 -0.0 3,240,022 + -2.7 3,240,152 - +0.6 3,240,152 - -2.0 3,240,191 - RR42_RS14985 0.21 -0.6 3,240,371 - -0.0 3,240,403 - -0.0 3,240,403 - +1.7 3,240,410 + -1.3 3,240,433 + -0.4 3,240,557 + +0.0 3,240,557 + -2.0 3,240,558 - -0.9 3,240,559 + -1.0 3,240,560 - +0.3 3,240,604 + RR42_RS14990 0.10 -1.6 3,240,604 + RR42_RS14990 0.10 -0.9 3,240,767 + RR42_RS14990 0.23 +0.4 3,240,767 + RR42_RS14990 0.23 -1.1 3,241,471 + RR42_RS14990 0.78 +0.0 3,241,471 + RR42_RS14990 0.78 +0.4 3,241,774 - -1.4 3,241,830 + +1.8
Or see this region's nucleotide sequence