Experiment: R2A with Chlorite 4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS09465 and RR42_RS09470 are separated by 20 nucleotides RR42_RS09470 and RR42_RS09475 overlap by 4 nucleotides RR42_RS09475 and RR42_RS09480 are separated by 23 nucleotides RR42_RS09480 and RR42_RS09485 overlap by 14 nucleotides
RR42_RS09465: RR42_RS09465 - bifunctional glyoxylate/hydroxypyruvate reductase B, at 2,008,115 to 2,009,092
_RS09465
RR42_RS09470: RR42_RS09470 - 2-dehydro-3-deoxygluconokinase, at 2,009,113 to 2,010,057
_RS09470
RR42_RS09475: RR42_RS09475 - glutamine ABC transporter ATP-binding protein, at 2,010,054 to 2,010,815
_RS09475
RR42_RS09480: RR42_RS09480 - amino acid ABC transporter ATP-binding protein, at 2,010,839 to 2,011,573
_RS09480
RR42_RS09485: RR42_RS09485 - amino acid ABC transporter permease, at 2,011,560 to 2,012,210
_RS09485
Position (kb)
2010
2011 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2009.078 kb on + strand at 2009.079 kb on - strand at 2009.349 kb on - strand, within RR42_RS09470 at 2009.735 kb on + strand, within RR42_RS09470 at 2010.109 kb on - strand at 2010.174 kb on + strand, within RR42_RS09475 at 2010.175 kb on - strand, within RR42_RS09475 at 2010.259 kb on + strand, within RR42_RS09475 at 2010.259 kb on + strand, within RR42_RS09475 at 2010.259 kb on + strand, within RR42_RS09475 at 2010.259 kb on + strand, within RR42_RS09475 at 2010.582 kb on - strand, within RR42_RS09475 at 2010.627 kb on + strand, within RR42_RS09475 at 2010.627 kb on + strand, within RR42_RS09475 at 2010.781 kb on + strand at 2010.782 kb on - strand at 2010.955 kb on + strand, within RR42_RS09480 at 2011.171 kb on - strand, within RR42_RS09480 at 2011.311 kb on + strand, within RR42_RS09480 at 2011.312 kb on - strand, within RR42_RS09480 at 2011.312 kb on - strand, within RR42_RS09480 at 2011.332 kb on - strand, within RR42_RS09480 at 2011.332 kb on - strand, within RR42_RS09480 at 2011.537 kb on - strand at 2011.537 kb on - strand at 2011.537 kb on - strand at 2011.634 kb on + strand, within RR42_RS09485 at 2011.635 kb on - strand, within RR42_RS09485 at 2011.635 kb on - strand, within RR42_RS09485 at 2011.635 kb on - strand, within RR42_RS09485
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chlorite 4 mM remove 2,009,078 + -0.5 2,009,079 - -0.1 2,009,349 - RR42_RS09470 0.25 +0.8 2,009,735 + RR42_RS09470 0.66 -1.0 2,010,109 - +0.4 2,010,174 + RR42_RS09475 0.16 -0.6 2,010,175 - RR42_RS09475 0.16 +0.3 2,010,259 + RR42_RS09475 0.27 -0.3 2,010,259 + RR42_RS09475 0.27 +0.4 2,010,259 + RR42_RS09475 0.27 -0.3 2,010,259 + RR42_RS09475 0.27 +2.7 2,010,582 - RR42_RS09475 0.69 +0.8 2,010,627 + RR42_RS09475 0.75 +0.2 2,010,627 + RR42_RS09475 0.75 -0.5 2,010,781 + -0.5 2,010,782 - +0.1 2,010,955 + RR42_RS09480 0.16 +0.1 2,011,171 - RR42_RS09480 0.45 -1.1 2,011,311 + RR42_RS09480 0.64 +1.3 2,011,312 - RR42_RS09480 0.64 +0.4 2,011,312 - RR42_RS09480 0.64 -0.8 2,011,332 - RR42_RS09480 0.67 -0.5 2,011,332 - RR42_RS09480 0.67 -0.6 2,011,537 - -0.2 2,011,537 - +0.2 2,011,537 - -0.5 2,011,634 + RR42_RS09485 0.11 -0.4 2,011,635 - RR42_RS09485 0.12 +3.1 2,011,635 - RR42_RS09485 0.12 +0.5 2,011,635 - RR42_RS09485 0.12 -0.4
Or see this region's nucleotide sequence