Experiment: R2A with Chlorite 4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06560 and RR42_RS06565 are separated by 95 nucleotides RR42_RS06565 and RR42_RS06570 are separated by 14 nucleotides RR42_RS06570 and RR42_RS06575 are separated by 202 nucleotides
RR42_RS06560: RR42_RS06560 - succinyl-CoA:3-ketoacid-CoA transferase, at 1,434,556 to 1,435,194
_RS06560
RR42_RS06565: RR42_RS06565 - metal-binding protein, at 1,435,290 to 1,435,784
_RS06565
RR42_RS06570: RR42_RS06570 - 3-hydroxybutyrate dehydrogenase, at 1,435,799 to 1,436,575
_RS06570
RR42_RS06575: RR42_RS06575 - alpha/beta hydrolase, at 1,436,778 to 1,437,665
_RS06575
Position (kb)
1435
1436
1437 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1435.309 kb on + strand at 1435.309 kb on + strand at 1435.310 kb on - strand at 1435.310 kb on - strand at 1435.658 kb on - strand, within RR42_RS06565 at 1435.658 kb on - strand, within RR42_RS06565 at 1435.967 kb on - strand, within RR42_RS06570 at 1436.119 kb on - strand, within RR42_RS06570 at 1436.119 kb on - strand, within RR42_RS06570 at 1436.119 kb on - strand, within RR42_RS06570 at 1436.119 kb on - strand, within RR42_RS06570 at 1436.570 kb on - strand at 1436.686 kb on + strand at 1436.687 kb on - strand at 1436.687 kb on - strand at 1436.701 kb on - strand at 1436.760 kb on + strand at 1436.760 kb on + strand at 1436.848 kb on - strand at 1436.848 kb on - strand at 1436.956 kb on - strand, within RR42_RS06575 at 1436.956 kb on - strand, within RR42_RS06575 at 1436.978 kb on - strand, within RR42_RS06575 at 1436.978 kb on - strand, within RR42_RS06575 at 1437.022 kb on - strand, within RR42_RS06575 at 1437.036 kb on + strand at 1437.037 kb on - strand, within RR42_RS06575 at 1437.037 kb on - strand, within RR42_RS06575 at 1437.037 kb on - strand, within RR42_RS06575 at 1437.037 kb on - strand, within RR42_RS06575 at 1437.037 kb on - strand, within RR42_RS06575 at 1437.222 kb on + strand, within RR42_RS06575 at 1437.222 kb on + strand, within RR42_RS06575 at 1437.223 kb on - strand, within RR42_RS06575 at 1437.223 kb on - strand, within RR42_RS06575 at 1437.388 kb on - strand, within RR42_RS06575 at 1437.499 kb on + strand, within RR42_RS06575
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chlorite 4 mM remove 1,435,309 + +0.1 1,435,309 + +0.2 1,435,310 - +1.0 1,435,310 - -0.3 1,435,658 - RR42_RS06565 0.74 +0.4 1,435,658 - RR42_RS06565 0.74 +0.0 1,435,967 - RR42_RS06570 0.22 +0.4 1,436,119 - RR42_RS06570 0.41 -0.1 1,436,119 - RR42_RS06570 0.41 +0.2 1,436,119 - RR42_RS06570 0.41 -0.6 1,436,119 - RR42_RS06570 0.41 +1.4 1,436,570 - +1.4 1,436,686 + -0.9 1,436,687 - -0.0 1,436,687 - -0.1 1,436,701 - +0.2 1,436,760 + +0.5 1,436,760 + +0.5 1,436,848 - +0.1 1,436,848 - -0.7 1,436,956 - RR42_RS06575 0.20 -1.5 1,436,956 - RR42_RS06575 0.20 -2.0 1,436,978 - RR42_RS06575 0.23 -0.0 1,436,978 - RR42_RS06575 0.23 -1.4 1,437,022 - RR42_RS06575 0.27 -2.0 1,437,036 + -1.0 1,437,037 - RR42_RS06575 0.29 -1.0 1,437,037 - RR42_RS06575 0.29 -1.2 1,437,037 - RR42_RS06575 0.29 -3.2 1,437,037 - RR42_RS06575 0.29 -0.4 1,437,037 - RR42_RS06575 0.29 -1.5 1,437,222 + RR42_RS06575 0.50 -0.0 1,437,222 + RR42_RS06575 0.50 +0.1 1,437,223 - RR42_RS06575 0.50 -1.6 1,437,223 - RR42_RS06575 0.50 -0.6 1,437,388 - RR42_RS06575 0.69 -0.1 1,437,499 + RR42_RS06575 0.81 -0.4
Or see this region's nucleotide sequence