Experiment: R2A with Chlorite 4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01160 and RR42_RS01165 overlap by 4 nucleotides RR42_RS01165 and RR42_RS01170 are separated by 40 nucleotides RR42_RS01170 and RR42_RS01175 are separated by 121 nucleotides
RR42_RS01160: RR42_RS01160 - homoserine O-acetyltransferase, at 266,379 to 267,551
_RS01160
RR42_RS01165: RR42_RS01165 - methionine biosynthesis protein MetW, at 267,548 to 268,159
_RS01165
RR42_RS01170: RR42_RS01170 - LysR family transcriptional regulator, at 268,200 to 269,117
_RS01170
RR42_RS01175: RR42_RS01175 - membrane protein, at 269,239 to 270,183
_RS01175
Position (kb) 268
269
270 Strain fitness (log2 ratio) -2
-1
0
1
2 at 267.231 kb on + strand, within RR42_RS01160 at 267.231 kb on + strand, within RR42_RS01160 at 267.249 kb on + strand, within RR42_RS01160 at 267.276 kb on + strand, within RR42_RS01160 at 267.439 kb on - strand at 267.439 kb on - strand at 267.439 kb on - strand at 267.496 kb on - strand at 267.516 kb on + strand at 267.517 kb on - strand at 267.517 kb on - strand at 267.520 kb on - strand at 267.541 kb on + strand at 267.541 kb on + strand at 267.542 kb on - strand at 267.542 kb on - strand at 267.571 kb on + strand at 267.701 kb on + strand, within RR42_RS01165 at 267.815 kb on - strand, within RR42_RS01165 at 267.984 kb on - strand, within RR42_RS01165 at 267.992 kb on + strand, within RR42_RS01165 at 267.992 kb on + strand, within RR42_RS01165 at 267.993 kb on - strand, within RR42_RS01165 at 268.009 kb on - strand, within RR42_RS01165 at 268.009 kb on - strand, within RR42_RS01165 at 268.237 kb on + strand at 268.238 kb on - strand at 268.245 kb on - strand at 268.285 kb on + strand at 268.286 kb on - strand at 268.305 kb on + strand, within RR42_RS01170 at 268.306 kb on - strand, within RR42_RS01170 at 268.306 kb on - strand, within RR42_RS01170 at 268.306 kb on - strand, within RR42_RS01170 at 268.306 kb on - strand, within RR42_RS01170 at 268.540 kb on + strand, within RR42_RS01170 at 268.540 kb on + strand, within RR42_RS01170 at 268.541 kb on - strand, within RR42_RS01170 at 268.541 kb on - strand, within RR42_RS01170 at 269.085 kb on + strand at 269.086 kb on - strand at 269.131 kb on + strand at 269.132 kb on - strand at 269.142 kb on - strand at 269.152 kb on + strand at 269.215 kb on + strand at 269.216 kb on - strand at 269.216 kb on - strand at 269.216 kb on - strand at 269.227 kb on + strand at 269.228 kb on - strand at 269.228 kb on - strand at 269.523 kb on + strand, within RR42_RS01175 at 269.566 kb on + strand, within RR42_RS01175 at 269.566 kb on + strand, within RR42_RS01175 at 269.566 kb on + strand, within RR42_RS01175 at 269.567 kb on - strand, within RR42_RS01175 at 269.596 kb on + strand, within RR42_RS01175 at 269.785 kb on + strand, within RR42_RS01175 at 269.956 kb on - strand, within RR42_RS01175 at 269.968 kb on + strand, within RR42_RS01175 at 269.968 kb on + strand, within RR42_RS01175 at 269.968 kb on + strand, within RR42_RS01175 at 269.968 kb on + strand, within RR42_RS01175 at 269.969 kb on - strand, within RR42_RS01175 at 269.969 kb on - strand, within RR42_RS01175 at 269.969 kb on - strand, within RR42_RS01175 at 269.969 kb on - strand, within RR42_RS01175 at 269.980 kb on + strand, within RR42_RS01175 at 269.980 kb on + strand, within RR42_RS01175 at 269.980 kb on + strand, within RR42_RS01175 at 269.980 kb on + strand, within RR42_RS01175 at 269.981 kb on - strand, within RR42_RS01175 at 269.981 kb on - strand, within RR42_RS01175 at 269.981 kb on - strand, within RR42_RS01175 at 269.981 kb on - strand, within RR42_RS01175 at 270.034 kb on + strand, within RR42_RS01175
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chlorite 4 mM remove 267,231 + RR42_RS01160 0.73 -1.6 267,231 + RR42_RS01160 0.73 -1.1 267,249 + RR42_RS01160 0.74 +0.4 267,276 + RR42_RS01160 0.76 -1.2 267,439 - +0.8 267,439 - -0.1 267,439 - +0.4 267,496 - +0.5 267,516 + +1.4 267,517 - +0.0 267,517 - +0.8 267,520 - +0.3 267,541 + -1.1 267,541 + +0.0 267,542 - -0.2 267,542 - -0.7 267,571 + -0.7 267,701 + RR42_RS01165 0.25 -0.0 267,815 - RR42_RS01165 0.44 -1.7 267,984 - RR42_RS01165 0.71 +1.6 267,992 + RR42_RS01165 0.73 +0.1 267,992 + RR42_RS01165 0.73 +0.1 267,993 - RR42_RS01165 0.73 -1.0 268,009 - RR42_RS01165 0.75 -1.1 268,009 - RR42_RS01165 0.75 +0.7 268,237 + +0.9 268,238 - +0.7 268,245 - -0.6 268,285 + -0.0 268,286 - -0.4 268,305 + RR42_RS01170 0.11 -0.3 268,306 - RR42_RS01170 0.12 +0.7 268,306 - RR42_RS01170 0.12 +0.7 268,306 - RR42_RS01170 0.12 +0.9 268,306 - RR42_RS01170 0.12 +0.2 268,540 + RR42_RS01170 0.37 +0.7 268,540 + RR42_RS01170 0.37 +2.5 268,541 - RR42_RS01170 0.37 +0.3 268,541 - RR42_RS01170 0.37 -0.6 269,085 + -1.0 269,086 - +0.4 269,131 + +1.1 269,132 - +1.0 269,142 - -0.4 269,152 + -0.6 269,215 + -0.7 269,216 - -0.1 269,216 - +0.6 269,216 - +0.4 269,227 + -0.4 269,228 - -0.3 269,228 - -0.4 269,523 + RR42_RS01175 0.30 +0.8 269,566 + RR42_RS01175 0.35 -0.1 269,566 + RR42_RS01175 0.35 -0.1 269,566 + RR42_RS01175 0.35 +1.3 269,567 - RR42_RS01175 0.35 +0.7 269,596 + RR42_RS01175 0.38 -0.5 269,785 + RR42_RS01175 0.58 +1.1 269,956 - RR42_RS01175 0.76 -0.7 269,968 + RR42_RS01175 0.77 -0.2 269,968 + RR42_RS01175 0.77 -0.4 269,968 + RR42_RS01175 0.77 -0.4 269,968 + RR42_RS01175 0.77 +0.2 269,969 - RR42_RS01175 0.77 +0.6 269,969 - RR42_RS01175 0.77 -0.2 269,969 - RR42_RS01175 0.77 +0.3 269,969 - RR42_RS01175 0.77 +0.3 269,980 + RR42_RS01175 0.78 -1.5 269,980 + RR42_RS01175 0.78 -2.1 269,980 + RR42_RS01175 0.78 +0.3 269,980 + RR42_RS01175 0.78 +0.4 269,981 - RR42_RS01175 0.79 +0.3 269,981 - RR42_RS01175 0.79 +0.5 269,981 - RR42_RS01175 0.79 +0.8 269,981 - RR42_RS01175 0.79 +0.4 270,034 + RR42_RS01175 0.84 +0.3
Or see this region's nucleotide sequence