Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_5102 and Psyr_5103 are separated by 1 nucleotides Psyr_5103 and Psyr_5104 overlap by 4 nucleotides
Psyr_5102: Psyr_5102 - ABC transporter, at 6,053,830 to 6,054,609
_5102
Psyr_5103: Psyr_5103 - Periplasmic binding protein, at 6,054,611 to 6,055,561
_5103
Psyr_5104: Psyr_5104 - transport system permease protein, at 6,055,558 to 6,056,601
_5104
Position (kb)
6054
6055
6056 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6053.612 kb on + strand at 6053.612 kb on + strand at 6053.618 kb on + strand at 6053.669 kb on + strand at 6053.670 kb on - strand at 6053.670 kb on - strand at 6053.767 kb on + strand at 6053.767 kb on + strand at 6053.767 kb on + strand at 6053.767 kb on + strand at 6053.767 kb on + strand at 6053.768 kb on - strand at 6053.998 kb on + strand, within Psyr_5102 at 6054.203 kb on + strand, within Psyr_5102 at 6054.204 kb on - strand, within Psyr_5102 at 6054.204 kb on - strand, within Psyr_5102 at 6054.651 kb on + strand at 6054.651 kb on + strand at 6054.652 kb on - strand at 6054.652 kb on - strand at 6054.652 kb on - strand at 6054.652 kb on - strand at 6054.850 kb on - strand, within Psyr_5103 at 6054.850 kb on - strand, within Psyr_5103 at 6054.883 kb on + strand, within Psyr_5103 at 6054.884 kb on - strand, within Psyr_5103 at 6054.884 kb on - strand, within Psyr_5103 at 6054.887 kb on + strand, within Psyr_5103 at 6054.887 kb on + strand, within Psyr_5103 at 6054.887 kb on + strand, within Psyr_5103 at 6054.888 kb on - strand, within Psyr_5103 at 6054.929 kb on + strand, within Psyr_5103 at 6054.929 kb on + strand, within Psyr_5103 at 6054.930 kb on - strand, within Psyr_5103 at 6054.930 kb on - strand, within Psyr_5103 at 6054.930 kb on - strand, within Psyr_5103 at 6054.930 kb on - strand, within Psyr_5103 at 6054.947 kb on + strand, within Psyr_5103 at 6054.947 kb on + strand, within Psyr_5103 at 6054.948 kb on - strand, within Psyr_5103 at 6054.948 kb on - strand, within Psyr_5103 at 6054.948 kb on - strand, within Psyr_5103 at 6055.010 kb on + strand, within Psyr_5103 at 6055.010 kb on + strand, within Psyr_5103 at 6055.011 kb on - strand, within Psyr_5103 at 6055.039 kb on - strand, within Psyr_5103 at 6055.039 kb on - strand, within Psyr_5103 at 6055.039 kb on - strand, within Psyr_5103 at 6055.073 kb on + strand, within Psyr_5103 at 6055.074 kb on - strand, within Psyr_5103 at 6055.074 kb on - strand, within Psyr_5103 at 6055.306 kb on - strand, within Psyr_5103 at 6055.466 kb on + strand, within Psyr_5103 at 6055.466 kb on + strand, within Psyr_5103 at 6055.467 kb on - strand at 6055.481 kb on + strand at 6055.481 kb on + strand at 6055.482 kb on - strand at 6055.482 kb on - strand at 6055.482 kb on - strand at 6055.482 kb on - strand at 6055.482 kb on - strand at 6055.778 kb on + strand, within Psyr_5104 at 6055.778 kb on + strand, within Psyr_5104 at 6055.778 kb on + strand, within Psyr_5104 at 6055.779 kb on - strand, within Psyr_5104 at 6055.782 kb on + strand, within Psyr_5104 at 6055.782 kb on + strand, within Psyr_5104 at 6055.782 kb on + strand, within Psyr_5104 at 6055.782 kb on + strand, within Psyr_5104 at 6055.783 kb on - strand, within Psyr_5104 at 6055.783 kb on - strand, within Psyr_5104 at 6056.216 kb on + strand, within Psyr_5104 at 6056.216 kb on + strand, within Psyr_5104 at 6056.313 kb on + strand, within Psyr_5104 at 6056.313 kb on + strand, within Psyr_5104 at 6056.357 kb on + strand, within Psyr_5104 at 6056.357 kb on + strand, within Psyr_5104 at 6056.545 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 6,053,612 + -0.0 6,053,612 + +1.4 6,053,618 + -0.1 6,053,669 + -0.0 6,053,670 - +0.5 6,053,670 - +0.1 6,053,767 + +0.4 6,053,767 + -2.5 6,053,767 + -0.4 6,053,767 + +0.7 6,053,767 + -0.2 6,053,768 - +2.1 6,053,998 + Psyr_5102 0.22 +0.1 6,054,203 + Psyr_5102 0.48 +0.0 6,054,204 - Psyr_5102 0.48 +0.3 6,054,204 - Psyr_5102 0.48 +0.2 6,054,651 + +0.3 6,054,651 + -0.1 6,054,652 - -1.0 6,054,652 - -0.6 6,054,652 - -1.3 6,054,652 - -2.0 6,054,850 - Psyr_5103 0.25 -0.1 6,054,850 - Psyr_5103 0.25 +0.3 6,054,883 + Psyr_5103 0.29 +0.5 6,054,884 - Psyr_5103 0.29 -1.0 6,054,884 - Psyr_5103 0.29 +1.0 6,054,887 + Psyr_5103 0.29 +0.1 6,054,887 + Psyr_5103 0.29 -0.5 6,054,887 + Psyr_5103 0.29 +1.1 6,054,888 - Psyr_5103 0.29 -0.5 6,054,929 + Psyr_5103 0.33 -0.6 6,054,929 + Psyr_5103 0.33 +0.8 6,054,930 - Psyr_5103 0.34 -0.5 6,054,930 - Psyr_5103 0.34 +1.1 6,054,930 - Psyr_5103 0.34 +0.6 6,054,930 - Psyr_5103 0.34 -0.5 6,054,947 + Psyr_5103 0.35 +0.3 6,054,947 + Psyr_5103 0.35 -1.3 6,054,948 - Psyr_5103 0.35 +0.6 6,054,948 - Psyr_5103 0.35 +0.1 6,054,948 - Psyr_5103 0.35 +1.3 6,055,010 + Psyr_5103 0.42 -0.1 6,055,010 + Psyr_5103 0.42 +1.8 6,055,011 - Psyr_5103 0.42 -0.8 6,055,039 - Psyr_5103 0.45 -0.5 6,055,039 - Psyr_5103 0.45 +1.2 6,055,039 - Psyr_5103 0.45 +0.9 6,055,073 + Psyr_5103 0.49 +1.1 6,055,074 - Psyr_5103 0.49 +1.7 6,055,074 - Psyr_5103 0.49 +0.3 6,055,306 - Psyr_5103 0.73 -0.1 6,055,466 + Psyr_5103 0.90 +0.8 6,055,466 + Psyr_5103 0.90 +0.1 6,055,467 - -1.7 6,055,481 + -0.2 6,055,481 + -0.5 6,055,482 - +0.2 6,055,482 - -0.4 6,055,482 - +1.5 6,055,482 - +2.7 6,055,482 - -1.5 6,055,778 + Psyr_5104 0.21 -0.3 6,055,778 + Psyr_5104 0.21 +2.3 6,055,778 + Psyr_5104 0.21 +0.3 6,055,779 - Psyr_5104 0.21 +0.1 6,055,782 + Psyr_5104 0.21 +0.1 6,055,782 + Psyr_5104 0.21 +0.8 6,055,782 + Psyr_5104 0.21 -0.8 6,055,782 + Psyr_5104 0.21 +1.6 6,055,783 - Psyr_5104 0.22 +0.1 6,055,783 - Psyr_5104 0.22 +1.1 6,056,216 + Psyr_5104 0.63 +0.7 6,056,216 + Psyr_5104 0.63 +1.5 6,056,313 + Psyr_5104 0.72 -0.4 6,056,313 + Psyr_5104 0.72 +0.1 6,056,357 + Psyr_5104 0.77 -0.0 6,056,357 + Psyr_5104 0.77 +0.0 6,056,545 - -0.6
Or see this region's nucleotide sequence