Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4713 and Psyr_4714 are separated by 15 nucleotides Psyr_4714 and Psyr_4715 overlap by 4 nucleotides Psyr_4715 and Psyr_4716 are separated by 50 nucleotides Psyr_4716 and Psyr_4717 are separated by 277 nucleotides
Psyr_4713: Psyr_4713 - Sarcosine oxidase, beta subunit, heterotetrameric, at 5,584,702 to 5,585,952
_4713
Psyr_4714: Psyr_4714 - Sarcosine oxidase, delta subunit, heterotetrameric, at 5,585,968 to 5,586,264
_4714
Psyr_4715: Psyr_4715 - Sarcosine oxidase, alpha subunit, heterotetrameric, at 5,586,261 to 5,589,281
_4715
Psyr_4716: Psyr_4716 - Sarcosine oxidase, gamma subunit, heterotetrameric, at 5,589,332 to 5,589,964
_4716
Psyr_4717: Psyr_4717 - formyltetrahydrofolate deformylase, at 5,590,242 to 5,591,099
_4717
Position (kb) 5586
5587
5588
5589
5590 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 5585.548 kb on + strand, within Psyr_4713 at 5585.548 kb on + strand, within Psyr_4713 at 5585.549 kb on - strand, within Psyr_4713 at 5585.549 kb on - strand, within Psyr_4713 at 5585.976 kb on + strand at 5585.976 kb on + strand at 5585.976 kb on + strand at 5585.976 kb on + strand at 5585.976 kb on + strand at 5585.976 kb on + strand at 5585.976 kb on + strand at 5585.977 kb on - strand at 5585.977 kb on - strand at 5585.977 kb on - strand at 5586.094 kb on + strand, within Psyr_4714 at 5586.094 kb on + strand, within Psyr_4714 at 5586.094 kb on + strand, within Psyr_4714 at 5586.094 kb on + strand, within Psyr_4714 at 5586.094 kb on + strand, within Psyr_4714 at 5586.095 kb on - strand, within Psyr_4714 at 5586.095 kb on - strand, within Psyr_4714 at 5586.095 kb on - strand, within Psyr_4714 at 5586.095 kb on - strand, within Psyr_4714 at 5586.095 kb on - strand, within Psyr_4714 at 5586.095 kb on - strand, within Psyr_4714 at 5586.095 kb on - strand, within Psyr_4714 at 5586.095 kb on - strand, within Psyr_4714 at 5586.095 kb on - strand, within Psyr_4714 at 5586.095 kb on - strand, within Psyr_4714 at 5586.143 kb on + strand, within Psyr_4714 at 5586.143 kb on + strand, within Psyr_4714 at 5586.167 kb on - strand, within Psyr_4714 at 5586.167 kb on - strand, within Psyr_4714 at 5586.196 kb on + strand, within Psyr_4714 at 5586.196 kb on + strand, within Psyr_4714 at 5586.197 kb on - strand, within Psyr_4714 at 5586.260 kb on + strand at 5586.260 kb on + strand at 5586.260 kb on + strand at 5586.260 kb on + strand at 5586.260 kb on + strand at 5586.260 kb on + strand at 5586.260 kb on + strand at 5586.261 kb on - strand at 5586.261 kb on - strand at 5586.261 kb on - strand at 5586.323 kb on + strand at 5586.324 kb on - strand at 5586.399 kb on - strand at 5586.420 kb on + strand at 5586.420 kb on + strand at 5586.421 kb on - strand at 5586.421 kb on - strand at 5586.538 kb on - strand at 5586.642 kb on + strand, within Psyr_4715 at 5586.642 kb on + strand, within Psyr_4715 at 5586.643 kb on - strand, within Psyr_4715 at 5586.645 kb on + strand, within Psyr_4715 at 5586.646 kb on - strand, within Psyr_4715 at 5586.660 kb on + strand, within Psyr_4715 at 5586.661 kb on - strand, within Psyr_4715 at 5586.684 kb on + strand, within Psyr_4715 at 5586.685 kb on - strand, within Psyr_4715 at 5586.693 kb on + strand, within Psyr_4715 at 5586.693 kb on + strand, within Psyr_4715 at 5586.694 kb on - strand, within Psyr_4715 at 5586.694 kb on - strand, within Psyr_4715 at 5586.694 kb on - strand, within Psyr_4715 at 5586.694 kb on - strand, within Psyr_4715 at 5586.750 kb on + strand, within Psyr_4715 at 5586.751 kb on - strand, within Psyr_4715 at 5587.016 kb on + strand, within Psyr_4715 at 5587.016 kb on + strand, within Psyr_4715 at 5587.017 kb on - strand, within Psyr_4715 at 5587.118 kb on - strand, within Psyr_4715 at 5587.155 kb on + strand, within Psyr_4715 at 5587.155 kb on + strand, within Psyr_4715 at 5587.156 kb on - strand, within Psyr_4715 at 5587.206 kb on + strand, within Psyr_4715 at 5587.207 kb on - strand, within Psyr_4715 at 5587.207 kb on - strand, within Psyr_4715 at 5587.207 kb on - strand, within Psyr_4715 at 5587.207 kb on - strand, within Psyr_4715 at 5587.207 kb on - strand, within Psyr_4715 at 5587.628 kb on + strand, within Psyr_4715 at 5587.628 kb on + strand, within Psyr_4715 at 5587.629 kb on - strand, within Psyr_4715 at 5587.629 kb on - strand, within Psyr_4715 at 5587.629 kb on - strand, within Psyr_4715 at 5587.656 kb on + strand, within Psyr_4715 at 5587.656 kb on + strand, within Psyr_4715 at 5588.043 kb on + strand, within Psyr_4715 at 5588.044 kb on - strand, within Psyr_4715 at 5588.044 kb on - strand, within Psyr_4715 at 5588.044 kb on - strand, within Psyr_4715 at 5588.331 kb on + strand, within Psyr_4715 at 5588.331 kb on + strand, within Psyr_4715 at 5588.332 kb on - strand, within Psyr_4715 at 5588.332 kb on - strand, within Psyr_4715 at 5588.535 kb on + strand, within Psyr_4715 at 5588.535 kb on + strand, within Psyr_4715 at 5588.535 kb on + strand, within Psyr_4715 at 5588.535 kb on + strand, within Psyr_4715 at 5588.536 kb on - strand, within Psyr_4715 at 5588.536 kb on - strand, within Psyr_4715 at 5588.724 kb on + strand, within Psyr_4715 at 5588.793 kb on + strand, within Psyr_4715 at 5588.794 kb on - strand, within Psyr_4715 at 5588.794 kb on - strand, within Psyr_4715 at 5588.794 kb on - strand, within Psyr_4715 at 5588.794 kb on - strand, within Psyr_4715 at 5589.132 kb on + strand at 5589.133 kb on - strand at 5589.174 kb on - strand at 5589.279 kb on + strand at 5589.280 kb on - strand at 5589.350 kb on + strand at 5589.664 kb on - strand, within Psyr_4716 at 5589.756 kb on + strand, within Psyr_4716 at 5589.757 kb on - strand, within Psyr_4716 at 5589.776 kb on + strand, within Psyr_4716 at 5589.776 kb on + strand, within Psyr_4716 at 5589.906 kb on - strand at 5589.941 kb on + strand at 5589.984 kb on + strand at 5589.984 kb on + strand at 5589.985 kb on - strand at 5589.985 kb on - strand at 5590.015 kb on + strand at 5590.015 kb on + strand at 5590.016 kb on - strand at 5590.036 kb on - strand at 5590.193 kb on - strand at 5590.224 kb on + strand at 5590.224 kb on + strand at 5590.241 kb on + strand at 5590.242 kb on - strand at 5590.242 kb on - strand at 5590.242 kb on - strand at 5590.242 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 5,585,548 + Psyr_4713 0.68 +0.4 5,585,548 + Psyr_4713 0.68 +1.1 5,585,549 - Psyr_4713 0.68 -0.7 5,585,549 - Psyr_4713 0.68 +0.4 5,585,976 + +1.9 5,585,976 + -0.2 5,585,976 + -0.5 5,585,976 + +0.2 5,585,976 + +0.0 5,585,976 + -0.6 5,585,976 + +0.9 5,585,977 - -0.2 5,585,977 - +0.8 5,585,977 - +0.5 5,586,094 + Psyr_4714 0.42 +0.5 5,586,094 + Psyr_4714 0.42 +0.0 5,586,094 + Psyr_4714 0.42 -0.3 5,586,094 + Psyr_4714 0.42 -1.8 5,586,094 + Psyr_4714 0.42 -0.6 5,586,095 - Psyr_4714 0.43 +0.3 5,586,095 - Psyr_4714 0.43 +0.7 5,586,095 - Psyr_4714 0.43 -0.4 5,586,095 - Psyr_4714 0.43 +0.9 5,586,095 - Psyr_4714 0.43 -1.2 5,586,095 - Psyr_4714 0.43 -0.1 5,586,095 - Psyr_4714 0.43 +1.7 5,586,095 - Psyr_4714 0.43 +0.4 5,586,095 - Psyr_4714 0.43 +0.2 5,586,095 - Psyr_4714 0.43 +0.1 5,586,143 + Psyr_4714 0.59 -0.1 5,586,143 + Psyr_4714 0.59 +0.4 5,586,167 - Psyr_4714 0.67 +1.2 5,586,167 - Psyr_4714 0.67 -0.6 5,586,196 + Psyr_4714 0.77 -0.1 5,586,196 + Psyr_4714 0.77 +0.6 5,586,197 - Psyr_4714 0.77 +0.5 5,586,260 + +1.1 5,586,260 + -0.2 5,586,260 + -0.2 5,586,260 + -0.1 5,586,260 + +0.4 5,586,260 + +0.0 5,586,260 + +0.9 5,586,261 - -0.4 5,586,261 - +1.5 5,586,261 - -1.1 5,586,323 + +0.1 5,586,324 - +0.5 5,586,399 - +0.4 5,586,420 + -0.5 5,586,420 + +1.4 5,586,421 - -0.3 5,586,421 - -1.3 5,586,538 - -0.1 5,586,642 + Psyr_4715 0.13 +0.8 5,586,642 + Psyr_4715 0.13 +0.3 5,586,643 - Psyr_4715 0.13 +1.1 5,586,645 + Psyr_4715 0.13 -1.9 5,586,646 - Psyr_4715 0.13 -0.9 5,586,660 + Psyr_4715 0.13 -0.3 5,586,661 - Psyr_4715 0.13 +0.8 5,586,684 + Psyr_4715 0.14 -0.1 5,586,685 - Psyr_4715 0.14 -1.0 5,586,693 + Psyr_4715 0.14 +0.9 5,586,693 + Psyr_4715 0.14 +3.8 5,586,694 - Psyr_4715 0.14 +0.6 5,586,694 - Psyr_4715 0.14 +1.3 5,586,694 - Psyr_4715 0.14 -1.8 5,586,694 - Psyr_4715 0.14 -0.2 5,586,750 + Psyr_4715 0.16 -0.4 5,586,751 - Psyr_4715 0.16 -0.2 5,587,016 + Psyr_4715 0.25 -0.6 5,587,016 + Psyr_4715 0.25 +0.5 5,587,017 - Psyr_4715 0.25 +1.2 5,587,118 - Psyr_4715 0.28 +0.1 5,587,155 + Psyr_4715 0.30 +1.1 5,587,155 + Psyr_4715 0.30 +1.4 5,587,156 - Psyr_4715 0.30 -0.1 5,587,206 + Psyr_4715 0.31 +0.2 5,587,207 - Psyr_4715 0.31 +0.6 5,587,207 - Psyr_4715 0.31 +1.6 5,587,207 - Psyr_4715 0.31 -0.9 5,587,207 - Psyr_4715 0.31 +0.1 5,587,207 - Psyr_4715 0.31 +0.4 5,587,628 + Psyr_4715 0.45 -0.5 5,587,628 + Psyr_4715 0.45 -1.3 5,587,629 - Psyr_4715 0.45 +0.2 5,587,629 - Psyr_4715 0.45 +0.1 5,587,629 - Psyr_4715 0.45 +0.9 5,587,656 + Psyr_4715 0.46 +0.2 5,587,656 + Psyr_4715 0.46 +0.4 5,588,043 + Psyr_4715 0.59 +1.1 5,588,044 - Psyr_4715 0.59 +1.0 5,588,044 - Psyr_4715 0.59 +0.1 5,588,044 - Psyr_4715 0.59 +0.6 5,588,331 + Psyr_4715 0.69 +0.4 5,588,331 + Psyr_4715 0.69 +0.1 5,588,332 - Psyr_4715 0.69 +0.0 5,588,332 - Psyr_4715 0.69 +0.9 5,588,535 + Psyr_4715 0.75 +0.4 5,588,535 + Psyr_4715 0.75 +0.1 5,588,535 + Psyr_4715 0.75 +0.3 5,588,535 + Psyr_4715 0.75 +1.6 5,588,536 - Psyr_4715 0.75 -0.1 5,588,536 - Psyr_4715 0.75 -0.5 5,588,724 + Psyr_4715 0.82 +0.1 5,588,793 + Psyr_4715 0.84 -0.5 5,588,794 - Psyr_4715 0.84 +0.9 5,588,794 - Psyr_4715 0.84 -1.4 5,588,794 - Psyr_4715 0.84 +0.1 5,588,794 - Psyr_4715 0.84 -1.8 5,589,132 + +0.4 5,589,133 - -0.8 5,589,174 - -1.6 5,589,279 + -0.5 5,589,280 - -0.4 5,589,350 + -1.6 5,589,664 - Psyr_4716 0.52 -0.1 5,589,756 + Psyr_4716 0.67 +0.9 5,589,757 - Psyr_4716 0.67 +0.3 5,589,776 + Psyr_4716 0.70 -2.2 5,589,776 + Psyr_4716 0.70 -1.0 5,589,906 - +0.2 5,589,941 + -0.8 5,589,984 + +0.0 5,589,984 + -0.4 5,589,985 - -0.6 5,589,985 - +0.3 5,590,015 + -0.2 5,590,015 + -0.6 5,590,016 - +1.2 5,590,036 - -1.4 5,590,193 - -0.7 5,590,224 + +0.5 5,590,224 + -0.8 5,590,241 + +0.2 5,590,242 - -0.1 5,590,242 - -0.7 5,590,242 - -0.2 5,590,242 - +0.2
Or see this region's nucleotide sequence