Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4617 and Psyr_4618 are separated by 177 nucleotides Psyr_4618 and Psyr_4619 overlap by 4 nucleotides Psyr_4619 and Psyr_4620 are separated by 114 nucleotides
Psyr_4617: Psyr_4617 - ISPsy9, transposase OrfA, at 5,475,383 to 5,475,922
_4617
Psyr_4618: Psyr_4618 - regulatory protein, LuxR:Response regulator receiver, at 5,476,100 to 5,476,729
_4618
Psyr_4619: Psyr_4619 - multi-sensor signal transduction histidine kinase, at 5,476,726 to 5,478,738
_4619
Psyr_4620: Psyr_4620 - conserved hypothetical protein, at 5,478,853 to 5,479,857
_4620
Position (kb) 5476
5477
5478
5479 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5476.055 kb on + strand at 5476.055 kb on + strand at 5476.055 kb on + strand at 5476.055 kb on + strand at 5476.055 kb on + strand at 5476.055 kb on + strand at 5476.126 kb on - strand at 5476.126 kb on - strand at 5476.126 kb on - strand at 5476.359 kb on + strand, within Psyr_4618 at 5476.360 kb on - strand, within Psyr_4618 at 5476.360 kb on - strand, within Psyr_4618 at 5476.423 kb on + strand, within Psyr_4618 at 5476.568 kb on - strand, within Psyr_4618 at 5476.764 kb on + strand at 5476.766 kb on + strand at 5476.766 kb on + strand at 5476.766 kb on + strand at 5476.766 kb on + strand at 5476.766 kb on + strand at 5476.767 kb on - strand at 5476.767 kb on - strand at 5476.767 kb on - strand at 5476.767 kb on - strand at 5476.767 kb on - strand at 5476.767 kb on - strand at 5477.331 kb on + strand, within Psyr_4619 at 5477.383 kb on + strand, within Psyr_4619 at 5477.383 kb on + strand, within Psyr_4619 at 5477.384 kb on - strand, within Psyr_4619 at 5477.438 kb on + strand, within Psyr_4619 at 5477.439 kb on - strand, within Psyr_4619 at 5477.439 kb on - strand, within Psyr_4619 at 5477.439 kb on - strand, within Psyr_4619 at 5477.439 kb on - strand, within Psyr_4619 at 5477.439 kb on - strand, within Psyr_4619 at 5477.439 kb on - strand, within Psyr_4619 at 5477.639 kb on + strand, within Psyr_4619 at 5477.673 kb on - strand, within Psyr_4619 at 5478.241 kb on - strand, within Psyr_4619 at 5478.344 kb on + strand, within Psyr_4619 at 5478.345 kb on - strand, within Psyr_4619 at 5478.670 kb on - strand at 5478.711 kb on + strand at 5478.742 kb on + strand at 5478.742 kb on + strand at 5478.742 kb on + strand at 5478.742 kb on + strand at 5478.743 kb on - strand at 5478.743 kb on - strand at 5478.743 kb on - strand at 5478.743 kb on - strand at 5478.743 kb on - strand at 5478.743 kb on - strand at 5478.743 kb on - strand at 5478.818 kb on - strand at 5478.848 kb on - strand at 5478.859 kb on + strand at 5478.860 kb on - strand at 5478.902 kb on + strand at 5479.022 kb on + strand, within Psyr_4620 at 5479.023 kb on - strand, within Psyr_4620 at 5479.257 kb on + strand, within Psyr_4620 at 5479.617 kb on + strand, within Psyr_4620 at 5479.618 kb on - strand, within Psyr_4620 at 5479.664 kb on + strand, within Psyr_4620 at 5479.665 kb on - strand, within Psyr_4620 at 5479.665 kb on - strand, within Psyr_4620 at 5479.665 kb on - strand, within Psyr_4620 at 5479.685 kb on - strand, within Psyr_4620 at 5479.685 kb on - strand, within Psyr_4620 at 5479.731 kb on + strand, within Psyr_4620
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 5,476,055 + -1.4 5,476,055 + -0.4 5,476,055 + -0.1 5,476,055 + +0.2 5,476,055 + -0.4 5,476,055 + -0.7 5,476,126 - -1.1 5,476,126 - -0.7 5,476,126 - -0.1 5,476,359 + Psyr_4618 0.41 -3.3 5,476,360 - Psyr_4618 0.41 -1.9 5,476,360 - Psyr_4618 0.41 -0.5 5,476,423 + Psyr_4618 0.51 -0.6 5,476,568 - Psyr_4618 0.74 -1.3 5,476,764 + +0.2 5,476,766 + +0.1 5,476,766 + -0.6 5,476,766 + -2.2 5,476,766 + -0.9 5,476,766 + -0.3 5,476,767 - -0.3 5,476,767 - -0.2 5,476,767 - -0.3 5,476,767 - -0.3 5,476,767 - +0.3 5,476,767 - -0.5 5,477,331 + Psyr_4619 0.30 -0.3 5,477,383 + Psyr_4619 0.33 +0.2 5,477,383 + Psyr_4619 0.33 -0.1 5,477,384 - Psyr_4619 0.33 -0.8 5,477,438 + Psyr_4619 0.35 +0.6 5,477,439 - Psyr_4619 0.35 -1.3 5,477,439 - Psyr_4619 0.35 +0.9 5,477,439 - Psyr_4619 0.35 +0.2 5,477,439 - Psyr_4619 0.35 +0.3 5,477,439 - Psyr_4619 0.35 -0.4 5,477,439 - Psyr_4619 0.35 -0.1 5,477,639 + Psyr_4619 0.45 -1.6 5,477,673 - Psyr_4619 0.47 -0.1 5,478,241 - Psyr_4619 0.75 +1.4 5,478,344 + Psyr_4619 0.80 -0.6 5,478,345 - Psyr_4619 0.80 -0.5 5,478,670 - -0.8 5,478,711 + -0.6 5,478,742 + -0.4 5,478,742 + -0.3 5,478,742 + -0.7 5,478,742 + -0.8 5,478,743 - -0.4 5,478,743 - +3.7 5,478,743 - -0.6 5,478,743 - -0.8 5,478,743 - -0.2 5,478,743 - +1.9 5,478,743 - +0.3 5,478,818 - +0.2 5,478,848 - +0.6 5,478,859 + -1.1 5,478,860 - -0.5 5,478,902 + -0.1 5,479,022 + Psyr_4620 0.17 -0.5 5,479,023 - Psyr_4620 0.17 +0.4 5,479,257 + Psyr_4620 0.40 +0.2 5,479,617 + Psyr_4620 0.76 -1.2 5,479,618 - Psyr_4620 0.76 -0.7 5,479,664 + Psyr_4620 0.81 -0.4 5,479,665 - Psyr_4620 0.81 -0.3 5,479,665 - Psyr_4620 0.81 +0.2 5,479,665 - Psyr_4620 0.81 -1.1 5,479,685 - Psyr_4620 0.83 +0.8 5,479,685 - Psyr_4620 0.83 +0.9 5,479,731 + Psyr_4620 0.87 +1.3
Or see this region's nucleotide sequence