Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4614 and Psyr_4615 are separated by 82 nucleotides Psyr_4615 and Psyr_4616 are separated by 425 nucleotides
Psyr_4614: Psyr_4614 - extracellular solute-binding protein, family 1, at 5,471,880 to 5,472,914
_4614
Psyr_4615: Psyr_4615 - Spermidine/putrescine ABC transporter ATP-binding subunit, at 5,472,997 to 5,474,121
_4615
Psyr_4616: Psyr_4616 - Integrase, catalytic region, at 5,474,547 to 5,475,383
_4616
Position (kb)
5472
5473
5474
5475 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5472.064 kb on + strand, within Psyr_4614 at 5472.065 kb on - strand, within Psyr_4614 at 5472.094 kb on + strand, within Psyr_4614 at 5472.095 kb on - strand, within Psyr_4614 at 5472.095 kb on - strand, within Psyr_4614 at 5472.095 kb on - strand, within Psyr_4614 at 5472.095 kb on - strand, within Psyr_4614 at 5472.095 kb on - strand, within Psyr_4614 at 5472.163 kb on + strand, within Psyr_4614 at 5472.164 kb on - strand, within Psyr_4614 at 5472.164 kb on - strand, within Psyr_4614 at 5472.164 kb on - strand, within Psyr_4614 at 5472.229 kb on + strand, within Psyr_4614 at 5472.229 kb on + strand, within Psyr_4614 at 5472.230 kb on - strand, within Psyr_4614 at 5472.230 kb on - strand, within Psyr_4614 at 5472.230 kb on - strand, within Psyr_4614 at 5472.266 kb on - strand, within Psyr_4614 at 5472.363 kb on + strand, within Psyr_4614 at 5472.364 kb on - strand, within Psyr_4614 at 5472.370 kb on + strand, within Psyr_4614 at 5472.370 kb on + strand, within Psyr_4614 at 5472.370 kb on + strand, within Psyr_4614 at 5472.370 kb on + strand, within Psyr_4614 at 5472.370 kb on + strand, within Psyr_4614 at 5472.371 kb on - strand, within Psyr_4614 at 5472.371 kb on - strand, within Psyr_4614 at 5472.371 kb on - strand, within Psyr_4614 at 5472.371 kb on - strand, within Psyr_4614 at 5472.421 kb on + strand, within Psyr_4614 at 5472.421 kb on + strand, within Psyr_4614 at 5472.421 kb on + strand, within Psyr_4614 at 5472.421 kb on + strand, within Psyr_4614 at 5472.421 kb on + strand, within Psyr_4614 at 5472.422 kb on - strand, within Psyr_4614 at 5472.422 kb on - strand at 5472.520 kb on + strand, within Psyr_4614 at 5472.672 kb on + strand, within Psyr_4614 at 5472.673 kb on - strand, within Psyr_4614 at 5472.673 kb on - strand, within Psyr_4614 at 5472.736 kb on + strand, within Psyr_4614 at 5472.736 kb on + strand, within Psyr_4614 at 5472.736 kb on + strand, within Psyr_4614 at 5472.737 kb on - strand, within Psyr_4614 at 5472.784 kb on + strand, within Psyr_4614 at 5472.785 kb on - strand, within Psyr_4614 at 5472.785 kb on - strand, within Psyr_4614 at 5472.943 kb on + strand at 5473.166 kb on + strand, within Psyr_4615 at 5473.166 kb on + strand, within Psyr_4615 at 5473.166 kb on + strand, within Psyr_4615 at 5473.166 kb on + strand, within Psyr_4615 at 5473.166 kb on + strand, within Psyr_4615 at 5473.167 kb on - strand at 5473.167 kb on - strand, within Psyr_4615 at 5473.167 kb on - strand, within Psyr_4615 at 5473.167 kb on - strand, within Psyr_4615 at 5473.167 kb on - strand, within Psyr_4615 at 5473.421 kb on + strand, within Psyr_4615 at 5473.421 kb on + strand, within Psyr_4615 at 5473.421 kb on + strand, within Psyr_4615 at 5473.422 kb on - strand, within Psyr_4615 at 5473.422 kb on - strand, within Psyr_4615 at 5473.422 kb on - strand, within Psyr_4615 at 5473.520 kb on + strand, within Psyr_4615 at 5473.520 kb on + strand, within Psyr_4615 at 5473.520 kb on + strand, within Psyr_4615 at 5473.521 kb on - strand, within Psyr_4615 at 5473.521 kb on - strand, within Psyr_4615 at 5473.521 kb on - strand, within Psyr_4615 at 5473.521 kb on - strand, within Psyr_4615 at 5473.521 kb on - strand, within Psyr_4615 at 5473.521 kb on - strand, within Psyr_4615 at 5473.521 kb on - strand, within Psyr_4615 at 5473.713 kb on + strand, within Psyr_4615 at 5473.835 kb on + strand, within Psyr_4615 at 5473.835 kb on + strand, within Psyr_4615 at 5473.835 kb on + strand, within Psyr_4615 at 5473.836 kb on - strand, within Psyr_4615 at 5473.836 kb on - strand, within Psyr_4615 at 5473.836 kb on - strand, within Psyr_4615 at 5473.836 kb on - strand, within Psyr_4615 at 5473.866 kb on + strand, within Psyr_4615 at 5474.051 kb on + strand at 5474.051 kb on + strand at 5474.052 kb on - strand at 5474.052 kb on - strand at 5474.159 kb on + strand at 5474.159 kb on + strand at 5474.160 kb on - strand at 5474.263 kb on + strand at 5474.264 kb on - strand at 5474.354 kb on + strand at 5474.354 kb on + strand at 5474.477 kb on + strand at 5474.477 kb on + strand at 5474.478 kb on - strand at 5474.478 kb on - strand at 5474.478 kb on - strand at 5474.478 kb on - strand at 5474.478 kb on - strand at 5474.478 kb on - strand at 5474.478 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 5,472,064 + Psyr_4614 0.18 -0.0 5,472,065 - Psyr_4614 0.18 -1.3 5,472,094 + Psyr_4614 0.21 -1.4 5,472,095 - Psyr_4614 0.21 -0.1 5,472,095 - Psyr_4614 0.21 +1.5 5,472,095 - Psyr_4614 0.21 -1.2 5,472,095 - Psyr_4614 0.21 +0.5 5,472,095 - Psyr_4614 0.21 +0.3 5,472,163 + Psyr_4614 0.27 -0.4 5,472,164 - Psyr_4614 0.27 -0.9 5,472,164 - Psyr_4614 0.27 -2.8 5,472,164 - Psyr_4614 0.27 +0.5 5,472,229 + Psyr_4614 0.34 -0.9 5,472,229 + Psyr_4614 0.34 -1.0 5,472,230 - Psyr_4614 0.34 +2.1 5,472,230 - Psyr_4614 0.34 +2.1 5,472,230 - Psyr_4614 0.34 -0.6 5,472,266 - Psyr_4614 0.37 -0.7 5,472,363 + Psyr_4614 0.47 +0.6 5,472,364 - Psyr_4614 0.47 -1.8 5,472,370 + Psyr_4614 0.47 -0.1 5,472,370 + Psyr_4614 0.47 -0.3 5,472,370 + Psyr_4614 0.47 -0.3 5,472,370 + Psyr_4614 0.47 +0.2 5,472,370 + Psyr_4614 0.47 +0.0 5,472,371 - Psyr_4614 0.47 -0.4 5,472,371 - Psyr_4614 0.47 -1.8 5,472,371 - Psyr_4614 0.47 -0.4 5,472,371 - Psyr_4614 0.47 -0.1 5,472,421 + Psyr_4614 0.52 -0.4 5,472,421 + Psyr_4614 0.52 +0.2 5,472,421 + Psyr_4614 0.52 +0.3 5,472,421 + Psyr_4614 0.52 +0.0 5,472,421 + Psyr_4614 0.52 -0.8 5,472,422 - Psyr_4614 0.52 -1.1 5,472,422 - -0.1 5,472,520 + Psyr_4614 0.62 +0.4 5,472,672 + Psyr_4614 0.77 +0.7 5,472,673 - Psyr_4614 0.77 -0.1 5,472,673 - Psyr_4614 0.77 +0.1 5,472,736 + Psyr_4614 0.83 -0.7 5,472,736 + Psyr_4614 0.83 -1.1 5,472,736 + Psyr_4614 0.83 +0.3 5,472,737 - Psyr_4614 0.83 -0.4 5,472,784 + Psyr_4614 0.87 -0.4 5,472,785 - Psyr_4614 0.87 -0.8 5,472,785 - Psyr_4614 0.87 -0.6 5,472,943 + +1.4 5,473,166 + Psyr_4615 0.15 +0.4 5,473,166 + Psyr_4615 0.15 +0.3 5,473,166 + Psyr_4615 0.15 -1.4 5,473,166 + Psyr_4615 0.15 -0.1 5,473,166 + Psyr_4615 0.15 -1.9 5,473,167 - -0.7 5,473,167 - Psyr_4615 0.15 -1.7 5,473,167 - Psyr_4615 0.15 -1.9 5,473,167 - Psyr_4615 0.15 -1.2 5,473,167 - Psyr_4615 0.15 +0.2 5,473,421 + Psyr_4615 0.38 +3.2 5,473,421 + Psyr_4615 0.38 +0.3 5,473,421 + Psyr_4615 0.38 -0.7 5,473,422 - Psyr_4615 0.38 -0.7 5,473,422 - Psyr_4615 0.38 +0.0 5,473,422 - Psyr_4615 0.38 +0.1 5,473,520 + Psyr_4615 0.46 +0.2 5,473,520 + Psyr_4615 0.46 +0.0 5,473,520 + Psyr_4615 0.46 -0.4 5,473,521 - Psyr_4615 0.47 +0.2 5,473,521 - Psyr_4615 0.47 -0.2 5,473,521 - Psyr_4615 0.47 -0.1 5,473,521 - Psyr_4615 0.47 +0.5 5,473,521 - Psyr_4615 0.47 +1.9 5,473,521 - Psyr_4615 0.47 +0.6 5,473,521 - Psyr_4615 0.47 -0.2 5,473,713 + Psyr_4615 0.64 +1.3 5,473,835 + Psyr_4615 0.74 +0.3 5,473,835 + Psyr_4615 0.74 +0.9 5,473,835 + Psyr_4615 0.74 -0.4 5,473,836 - Psyr_4615 0.75 +0.8 5,473,836 - Psyr_4615 0.75 -0.9 5,473,836 - Psyr_4615 0.75 +0.8 5,473,836 - Psyr_4615 0.75 -0.1 5,473,866 + Psyr_4615 0.77 +0.0 5,474,051 + -0.3 5,474,051 + -0.3 5,474,052 - -1.6 5,474,052 - +0.8 5,474,159 + -1.1 5,474,159 + -1.3 5,474,160 - -0.7 5,474,263 + +0.7 5,474,264 - -0.3 5,474,354 + -0.8 5,474,354 + -0.4 5,474,477 + -0.2 5,474,477 + +0.6 5,474,478 - -0.5 5,474,478 - +0.5 5,474,478 - -0.0 5,474,478 - +0.0 5,474,478 - +2.1 5,474,478 - -0.7 5,474,478 - -0.9
Or see this region's nucleotide sequence