Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4577 and Psyr_4578 are separated by 11 nucleotides Psyr_4578 and Psyr_4579 are separated by 34 nucleotides Psyr_4579 and Psyr_4580 overlap by 4 nucleotides
Psyr_4577: Psyr_4577 - Cyclic nucleotide-binding:Bacterial regulatory protein, Crp, at 5,439,759 to 5,440,403
_4577
Psyr_4578: Psyr_4578 - conserved hypothetical protein, at 5,440,415 to 5,441,110
_4578
Psyr_4579: Psyr_4579 - indole-3-glycerol phosphate synthase, at 5,441,145 to 5,441,981
_4579
Psyr_4580: Psyr_4580 - anthranilate phosphoribosyltransferase, at 5,441,978 to 5,443,039
_4580
Position (kb) 5441
5442 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5440.151 kb on + strand, within Psyr_4577 at 5440.151 kb on + strand, within Psyr_4577 at 5440.151 kb on + strand, within Psyr_4577 at 5440.152 kb on - strand, within Psyr_4577 at 5440.152 kb on - strand, within Psyr_4577 at 5440.413 kb on - strand at 5440.413 kb on - strand at 5440.413 kb on - strand at 5440.488 kb on + strand, within Psyr_4578 at 5440.488 kb on + strand, within Psyr_4578 at 5440.488 kb on + strand, within Psyr_4578 at 5440.778 kb on + strand, within Psyr_4578 at 5440.779 kb on - strand, within Psyr_4578 at 5440.779 kb on - strand, within Psyr_4578 at 5440.779 kb on - strand, within Psyr_4578 at 5440.799 kb on + strand, within Psyr_4578 at 5440.799 kb on + strand, within Psyr_4578 at 5440.799 kb on + strand, within Psyr_4578 at 5440.800 kb on - strand, within Psyr_4578 at 5440.800 kb on - strand, within Psyr_4578 at 5440.801 kb on + strand, within Psyr_4578 at 5440.801 kb on + strand, within Psyr_4578 at 5440.836 kb on + strand, within Psyr_4578 at 5440.837 kb on - strand, within Psyr_4578 at 5440.837 kb on - strand, within Psyr_4578 at 5441.087 kb on + strand at 5441.087 kb on + strand at 5441.087 kb on + strand at 5441.087 kb on + strand at 5441.087 kb on + strand at 5441.087 kb on + strand at 5441.087 kb on + strand at 5441.087 kb on + strand at 5441.087 kb on + strand at 5441.087 kb on + strand at 5441.087 kb on + strand at 5441.087 kb on + strand at 5441.087 kb on + strand at 5441.087 kb on + strand at 5441.087 kb on + strand at 5441.087 kb on + strand at 5441.087 kb on + strand at 5441.087 kb on + strand at 5441.087 kb on + strand at 5441.087 kb on + strand at 5441.087 kb on + strand at 5441.087 kb on + strand at 5441.087 kb on + strand at 5441.087 kb on + strand at 5441.087 kb on + strand at 5441.087 kb on + strand at 5441.087 kb on + strand at 5441.087 kb on + strand at 5441.087 kb on + strand at 5441.087 kb on + strand at 5441.087 kb on + strand at 5441.087 kb on + strand at 5441.088 kb on - strand at 5441.088 kb on - strand at 5441.088 kb on - strand at 5441.088 kb on - strand at 5441.088 kb on - strand at 5441.088 kb on - strand at 5441.088 kb on - strand at 5441.088 kb on - strand at 5441.088 kb on - strand at 5441.088 kb on - strand at 5441.088 kb on - strand at 5441.088 kb on - strand at 5441.088 kb on - strand at 5441.088 kb on - strand at 5441.088 kb on - strand at 5441.088 kb on - strand at 5441.088 kb on - strand at 5441.166 kb on + strand at 5441.167 kb on - strand at 5441.257 kb on + strand, within Psyr_4579 at 5441.360 kb on + strand, within Psyr_4579 at 5441.360 kb on + strand, within Psyr_4579 at 5441.384 kb on + strand, within Psyr_4579 at 5441.384 kb on + strand, within Psyr_4579 at 5441.384 kb on + strand, within Psyr_4579 at 5441.384 kb on + strand, within Psyr_4579 at 5441.385 kb on - strand, within Psyr_4579 at 5441.385 kb on - strand, within Psyr_4579 at 5441.385 kb on - strand, within Psyr_4579 at 5441.475 kb on + strand, within Psyr_4579 at 5441.475 kb on + strand, within Psyr_4579 at 5441.475 kb on + strand, within Psyr_4579 at 5441.475 kb on + strand, within Psyr_4579 at 5441.475 kb on + strand, within Psyr_4579 at 5441.476 kb on - strand, within Psyr_4579 at 5441.476 kb on - strand, within Psyr_4579 at 5441.659 kb on + strand, within Psyr_4579 at 5441.729 kb on - strand, within Psyr_4579 at 5441.965 kb on - strand at 5442.114 kb on + strand, within Psyr_4580 at 5442.115 kb on - strand, within Psyr_4580 at 5442.286 kb on - strand, within Psyr_4580 at 5442.493 kb on + strand, within Psyr_4580 at 5442.493 kb on + strand, within Psyr_4580 at 5442.494 kb on - strand, within Psyr_4580 at 5442.633 kb on + strand, within Psyr_4580 at 5442.634 kb on - strand, within Psyr_4580
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 5,440,151 + Psyr_4577 0.61 -2.1 5,440,151 + Psyr_4577 0.61 -0.1 5,440,151 + Psyr_4577 0.61 +0.4 5,440,152 - Psyr_4577 0.61 -0.7 5,440,152 - Psyr_4577 0.61 -0.6 5,440,413 - +0.3 5,440,413 - +0.3 5,440,413 - -0.8 5,440,488 + Psyr_4578 0.10 -0.7 5,440,488 + Psyr_4578 0.10 -0.7 5,440,488 + Psyr_4578 0.10 +0.7 5,440,778 + Psyr_4578 0.52 -0.7 5,440,779 - Psyr_4578 0.52 -0.2 5,440,779 - Psyr_4578 0.52 +0.1 5,440,779 - Psyr_4578 0.52 -0.5 5,440,799 + Psyr_4578 0.55 -0.8 5,440,799 + Psyr_4578 0.55 -1.9 5,440,799 + Psyr_4578 0.55 +1.1 5,440,800 - Psyr_4578 0.55 +0.9 5,440,800 - Psyr_4578 0.55 -1.1 5,440,801 + Psyr_4578 0.55 +0.2 5,440,801 + Psyr_4578 0.55 -0.6 5,440,836 + Psyr_4578 0.60 +0.1 5,440,837 - Psyr_4578 0.61 -0.4 5,440,837 - Psyr_4578 0.61 -1.2 5,441,087 + +2.3 5,441,087 + -1.1 5,441,087 + -1.0 5,441,087 + -0.7 5,441,087 + +0.4 5,441,087 + +0.2 5,441,087 + +1.3 5,441,087 + +0.5 5,441,087 + -0.4 5,441,087 + -0.5 5,441,087 + +0.5 5,441,087 + -1.5 5,441,087 + -0.7 5,441,087 + -2.4 5,441,087 + -0.6 5,441,087 + +0.2 5,441,087 + +0.3 5,441,087 + +0.9 5,441,087 + -1.1 5,441,087 + -1.3 5,441,087 + +0.0 5,441,087 + +1.7 5,441,087 + +0.3 5,441,087 + -1.7 5,441,087 + +0.9 5,441,087 + +0.6 5,441,087 + -2.5 5,441,087 + -1.4 5,441,087 + +0.2 5,441,087 + -1.3 5,441,087 + -0.7 5,441,087 + -0.3 5,441,088 - -1.3 5,441,088 - +1.3 5,441,088 - -0.5 5,441,088 - -0.3 5,441,088 - -0.1 5,441,088 - +1.2 5,441,088 - +0.5 5,441,088 - -1.4 5,441,088 - +0.4 5,441,088 - -0.5 5,441,088 - -0.1 5,441,088 - +0.0 5,441,088 - +0.5 5,441,088 - -0.9 5,441,088 - -0.5 5,441,088 - -3.4 5,441,088 - -2.0 5,441,166 + +0.4 5,441,167 - +0.5 5,441,257 + Psyr_4579 0.13 +1.1 5,441,360 + Psyr_4579 0.26 -0.9 5,441,360 + Psyr_4579 0.26 -1.1 5,441,384 + Psyr_4579 0.29 -0.7 5,441,384 + Psyr_4579 0.29 -0.1 5,441,384 + Psyr_4579 0.29 -0.1 5,441,384 + Psyr_4579 0.29 -2.2 5,441,385 - Psyr_4579 0.29 -0.1 5,441,385 - Psyr_4579 0.29 -0.1 5,441,385 - Psyr_4579 0.29 -0.7 5,441,475 + Psyr_4579 0.39 -0.7 5,441,475 + Psyr_4579 0.39 +0.2 5,441,475 + Psyr_4579 0.39 -1.2 5,441,475 + Psyr_4579 0.39 +0.5 5,441,475 + Psyr_4579 0.39 +0.3 5,441,476 - Psyr_4579 0.40 -0.9 5,441,476 - Psyr_4579 0.40 -0.6 5,441,659 + Psyr_4579 0.61 +0.1 5,441,729 - Psyr_4579 0.70 -0.3 5,441,965 - -0.0 5,442,114 + Psyr_4580 0.13 -0.3 5,442,115 - Psyr_4580 0.13 -0.4 5,442,286 - Psyr_4580 0.29 -0.7 5,442,493 + Psyr_4580 0.48 -0.7 5,442,493 + Psyr_4580 0.48 +0.6 5,442,494 - Psyr_4580 0.49 -0.1 5,442,633 + Psyr_4580 0.62 -0.7 5,442,634 - Psyr_4580 0.62 -0.7
Or see this region's nucleotide sequence