Experiment: KB with Berberine 250 ug/ml 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Psyr_4385 and Psyr_4386 are separated by 330 nucleotides Psyr_4386 and Psyr_4387 are separated by 95 nucleotides 
        Psyr_4385: Psyr_4385 - Alpha-2-macroglobulin, N-terminal:Alpha-2-macroglobulin, N-terminal, at 5,242,625 to 5,247,583 
        _4385 
         
        
        Psyr_4386: Psyr_4386 - conserved hypothetical protein, at 5,247,914 to 5,248,207 
        _4386 
         
        
        Psyr_4387: Psyr_4387 - Glycosyl transferase, family 51:Penicillin-binding protein, transpeptidase:Penicillin-binding, C-terminal, at 5,248,303 to 5,250,651 
        _4387 
         Position (kb) 5247 
5248 
5249 Strain fitness (log2 ratio) -2 
-1 
0 
1 
2 at 5247.149 kb on + strand at 5247.149 kb on + strand at 5247.150 kb on - strand at 5247.411 kb on - strand at 5247.411 kb on - strand at 5247.458 kb on + strand at 5247.459 kb on - strand at 5247.459 kb on - strand at 5247.459 kb on - strand at 5247.612 kb on + strand at 5247.704 kb on - strand at 5247.759 kb on + strand at 5247.767 kb on + strand at 5247.901 kb on - strand at 5248.008 kb on + strand, within Psyr_4386 at 5248.008 kb on + strand, within Psyr_4386 at 5248.008 kb on + strand, within Psyr_4386 at 5248.008 kb on + strand, within Psyr_4386 at 5248.009 kb on - strand, within Psyr_4386 at 5248.009 kb on - strand, within Psyr_4386 at 5248.199 kb on + strand at 5248.199 kb on + strand at 5248.199 kb on + strand at 5248.199 kb on + strand at 5248.199 kb on + strand at 5248.199 kb on + strand at 5248.200 kb on - strand at 5248.200 kb on - strand at 5248.200 kb on - strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.288 kb on + strand at 5248.288 kb on + strand at 5248.493 kb on + strand at 5248.494 kb on - strand at 5248.494 kb on - strand at 5248.532 kb on - strand at 5248.534 kb on + strand at 5248.534 kb on + strand at 5248.535 kb on - strand at 5248.535 kb on - strand at 5248.535 kb on - strand at 5248.555 kb on + strand, within Psyr_4387 at 5248.556 kb on - strand, within Psyr_4387 at 5248.556 kb on - strand, within Psyr_4387 at 5248.573 kb on + strand, within Psyr_4387 at 5248.574 kb on - strand, within Psyr_4387 at 5248.643 kb on + strand, within Psyr_4387 at 5248.644 kb on - strand, within Psyr_4387 at 5248.644 kb on - strand, within Psyr_4387 at 5248.704 kb on + strand, within Psyr_4387 at 5248.704 kb on + strand, within Psyr_4387 at 5248.704 kb on + strand, within Psyr_4387 at 5248.704 kb on + strand, within Psyr_4387 at 5248.704 kb on + strand, within Psyr_4387 at 5248.704 kb on + strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.786 kb on + strand, within Psyr_4387 at 5248.787 kb on - strand, within Psyr_4387 at 5248.918 kb on - strand, within Psyr_4387  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction KB with Berberine 250 ug/ml remove 5,247,149 +  +0.7 5,247,149 +  -0.3 5,247,150 -  +0.7 5,247,411 -  -0.1 5,247,411 -  -0.2 5,247,458 +  -1.2 5,247,459 -  -0.7 5,247,459 -  -0.1 5,247,459 -  -1.0 5,247,612 +  -0.2 5,247,704 -  -0.1 5,247,759 +  +0.7 5,247,767 +  -1.4 5,247,901 -  +0.5 5,248,008 +  Psyr_4386 0.32  -0.7 5,248,008 +  Psyr_4386 0.32  +0.4 5,248,008 +  Psyr_4386 0.32  -0.8 5,248,008 +  Psyr_4386 0.32  -0.1 5,248,009 -  Psyr_4386 0.32  -1.2 5,248,009 -  Psyr_4386 0.32  -0.9 5,248,199 +  -1.6 5,248,199 +  -2.3 5,248,199 +  -1.6 5,248,199 +  -0.4 5,248,199 +  -0.7 5,248,199 +  +0.6 5,248,200 -  -0.1 5,248,200 -  -0.6 5,248,200 -  -0.8 5,248,207 +  -1.9 5,248,207 +  +1.3 5,248,207 +  -0.5 5,248,207 +  -0.5 5,248,207 +  -0.8 5,248,207 +  -0.4 5,248,207 +  -0.1 5,248,207 +  +1.5 5,248,207 +  -0.9 5,248,207 +  -0.7 5,248,207 +  +0.7 5,248,207 +  +0.3 5,248,207 +  +0.2 5,248,207 +  +0.3 5,248,207 +  +0.9 5,248,207 +  -1.3 5,248,207 +  -1.3 5,248,207 +  +0.3 5,248,207 +  +0.9 5,248,207 +  -0.8 5,248,207 +  +2.0 5,248,208 -  +0.1 5,248,208 -  -0.5 5,248,208 -  +0.6 5,248,208 -  +0.5 5,248,208 -  -0.3 5,248,208 -  +0.0 5,248,208 -  +0.2 5,248,208 -  -0.5 5,248,208 -  -0.1 5,248,208 -  +0.1 5,248,208 -  +0.4 5,248,208 -  -0.2 5,248,208 -  +0.1 5,248,208 -  -0.2 5,248,208 -  -0.1 5,248,208 -  -0.1 5,248,208 -  +0.6 5,248,208 -  +0.1 5,248,208 -  +0.6 5,248,208 -  -1.3 5,248,208 -  +1.4 5,248,208 -  +1.5 5,248,208 -  +1.4 5,248,208 -  +0.6 5,248,208 -  +0.6 5,248,208 -  +1.9 5,248,208 -  +1.8 5,248,208 -  +0.3 5,248,208 -  -1.3 5,248,208 -  -2.6 5,248,211 +  -0.7 5,248,211 +  +1.3 5,248,211 +  +0.3 5,248,211 +  -0.1 5,248,211 +  +0.4 5,248,211 +  +0.3 5,248,211 +  -0.4 5,248,211 +  -1.1 5,248,211 +  -0.6 5,248,211 +  -0.1 5,248,211 +  -1.0 5,248,211 +  +0.5 5,248,211 +  -0.1 5,248,211 +  -1.0 5,248,211 +  -0.7 5,248,211 +  +0.3 5,248,211 +  -2.2 5,248,211 +  -1.9 5,248,211 +  +1.9 5,248,211 +  +0.4 5,248,212 -  +0.2 5,248,212 -  +0.2 5,248,212 -  -0.0 5,248,212 -  +0.5 5,248,212 -  +0.4 5,248,212 -  +0.4 5,248,212 -  -0.4 5,248,212 -  +0.4 5,248,212 -  +0.9 5,248,212 -  +0.0 5,248,212 -  -1.6 5,248,212 -  +0.1 5,248,212 -  -0.3 5,248,212 -  +0.8 5,248,212 -  -0.1 5,248,212 -  +0.1 5,248,212 -  +0.0 5,248,212 -  +0.6 5,248,212 -  -0.4 5,248,212 -  +0.5 5,248,212 -  -0.7 5,248,212 -  -0.6 5,248,212 -  -1.5 5,248,288 +  -0.7 5,248,288 +  +0.3 5,248,493 +  -0.4 5,248,494 -  -0.3 5,248,494 -  -0.7 5,248,532 -  +0.6 5,248,534 +  -0.0 5,248,534 +  -0.4 5,248,535 -  +0.6 5,248,535 -  +0.2 5,248,535 -  -0.7 5,248,555 +  Psyr_4387 0.11  -0.2 5,248,556 -  Psyr_4387 0.11  +0.1 5,248,556 -  Psyr_4387 0.11  +0.6 5,248,573 +  Psyr_4387 0.11  -0.6 5,248,574 -  Psyr_4387 0.12  +0.5 5,248,643 +  Psyr_4387 0.14  -1.0 5,248,644 -  Psyr_4387 0.15  -1.7 5,248,644 -  Psyr_4387 0.15  +0.2 5,248,704 +  Psyr_4387 0.17  -0.4 5,248,704 +  Psyr_4387 0.17  -0.4 5,248,704 +  Psyr_4387 0.17  +0.2 5,248,704 +  Psyr_4387 0.17  +0.1 5,248,704 +  Psyr_4387 0.17  -1.1 5,248,704 +  Psyr_4387 0.17  -1.7 5,248,705 -  Psyr_4387 0.17  -0.9 5,248,705 -  Psyr_4387 0.17  +0.8 5,248,705 -  Psyr_4387 0.17  +1.9 5,248,705 -  Psyr_4387 0.17  -0.1 5,248,705 -  Psyr_4387 0.17  +0.1 5,248,705 -  Psyr_4387 0.17  -0.7 5,248,705 -  Psyr_4387 0.17  +0.4 5,248,705 -  Psyr_4387 0.17  +0.4 5,248,705 -  Psyr_4387 0.17  +0.6 5,248,705 -  Psyr_4387 0.17  +1.1 5,248,705 -  Psyr_4387 0.17  +0.6 5,248,705 -  Psyr_4387 0.17  +1.5 5,248,786 +  Psyr_4387 0.21  +0.0 5,248,787 -  Psyr_4387 0.21  -0.7 5,248,918 -  Psyr_4387 0.26  -0.2 
Or see this region's nucleotide sequence