Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3004 and Psyr_3005 are separated by 80 nucleotides Psyr_3005 and Psyr_3006 are separated by 126 nucleotides Psyr_3006 and Psyr_3007 overlap by 6 nucleotides Psyr_3007 and Psyr_3008 overlap by 4 nucleotides
Psyr_3004: Psyr_3004 - transcriptional regulator, LysR family, at 3,599,856 to 3,600,815
_3004
Psyr_3005: Psyr_3005 - 3-oxoadipate enol-lactonase, at 3,600,896 to 3,601,687
_3005
Psyr_3006: Psyr_3006 - Protein of unknown function DUF419, at 3,601,814 to 3,602,182
_3006
Psyr_3007: Psyr_3007 - Protein of unknown function DUF1294, at 3,602,177 to 3,602,611
_3007
Psyr_3008: Psyr_3008 - Undecaprenyl-diphosphatase, at 3,602,608 to 3,603,438
_3008
Position (kb) 3600
3601
3602 Strain fitness (log2 ratio) -2
-1
0
1
2 at 3599.900 kb on - strand at 3600.171 kb on + strand, within Psyr_3004 at 3600.172 kb on - strand, within Psyr_3004 at 3600.435 kb on - strand, within Psyr_3004 at 3600.619 kb on + strand, within Psyr_3004 at 3600.619 kb on + strand, within Psyr_3004 at 3600.622 kb on + strand, within Psyr_3004 at 3600.622 kb on + strand, within Psyr_3004 at 3600.622 kb on + strand, within Psyr_3004 at 3600.845 kb on - strand at 3600.845 kb on - strand at 3600.845 kb on - strand at 3600.998 kb on - strand, within Psyr_3005 at 3601.448 kb on + strand, within Psyr_3005 at 3601.485 kb on + strand, within Psyr_3005 at 3601.651 kb on - strand at 3601.720 kb on + strand at 3601.721 kb on - strand at 3601.721 kb on - strand at 3601.740 kb on + strand at 3601.740 kb on + strand at 3601.740 kb on + strand at 3601.740 kb on + strand at 3601.741 kb on - strand at 3601.741 kb on - strand at 3601.741 kb on - strand at 3601.741 kb on - strand at 3601.741 kb on - strand at 3601.741 kb on - strand at 3601.741 kb on - strand at 3601.741 kb on - strand at 3601.741 kb on - strand at 3601.741 kb on - strand at 3601.741 kb on - strand at 3601.741 kb on - strand at 3601.895 kb on + strand, within Psyr_3006 at 3601.895 kb on + strand, within Psyr_3006 at 3601.895 kb on + strand, within Psyr_3006 at 3601.896 kb on - strand, within Psyr_3006 at 3601.896 kb on - strand, within Psyr_3006 at 3601.896 kb on - strand, within Psyr_3006 at 3602.028 kb on + strand, within Psyr_3006 at 3602.028 kb on + strand, within Psyr_3006 at 3602.029 kb on - strand, within Psyr_3006 at 3602.029 kb on - strand, within Psyr_3006 at 3602.079 kb on + strand, within Psyr_3006 at 3602.079 kb on + strand, within Psyr_3006 at 3602.178 kb on + strand at 3602.178 kb on + strand at 3602.178 kb on + strand at 3602.178 kb on + strand at 3602.178 kb on + strand at 3602.178 kb on + strand at 3602.179 kb on - strand at 3602.179 kb on - strand at 3602.179 kb on - strand at 3602.179 kb on - strand at 3602.265 kb on - strand, within Psyr_3007 at 3602.265 kb on - strand, within Psyr_3007 at 3602.502 kb on + strand, within Psyr_3007
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 3,599,900 - -1.0 3,600,171 + Psyr_3004 0.33 -2.0 3,600,172 - Psyr_3004 0.33 -1.3 3,600,435 - Psyr_3004 0.60 +0.5 3,600,619 + Psyr_3004 0.79 -0.4 3,600,619 + Psyr_3004 0.79 -1.1 3,600,622 + Psyr_3004 0.80 -0.1 3,600,622 + Psyr_3004 0.80 +2.0 3,600,622 + Psyr_3004 0.80 +0.1 3,600,845 - +1.6 3,600,845 - +0.3 3,600,845 - -0.6 3,600,998 - Psyr_3005 0.13 -1.8 3,601,448 + Psyr_3005 0.70 -0.3 3,601,485 + Psyr_3005 0.74 -0.9 3,601,651 - -1.1 3,601,720 + -1.4 3,601,721 - -0.1 3,601,721 - +0.0 3,601,740 + +0.3 3,601,740 + -0.2 3,601,740 + +0.6 3,601,740 + -0.6 3,601,741 - -0.1 3,601,741 - -0.1 3,601,741 - -0.4 3,601,741 - -0.8 3,601,741 - -1.7 3,601,741 - -1.2 3,601,741 - -0.4 3,601,741 - -0.9 3,601,741 - +0.0 3,601,741 - +0.1 3,601,741 - -0.5 3,601,741 - +0.8 3,601,895 + Psyr_3006 0.22 -0.4 3,601,895 + Psyr_3006 0.22 +1.4 3,601,895 + Psyr_3006 0.22 -0.1 3,601,896 - Psyr_3006 0.22 -0.3 3,601,896 - Psyr_3006 0.22 +0.6 3,601,896 - Psyr_3006 0.22 +0.8 3,602,028 + Psyr_3006 0.58 -0.3 3,602,028 + Psyr_3006 0.58 -1.2 3,602,029 - Psyr_3006 0.58 -0.7 3,602,029 - Psyr_3006 0.58 -0.6 3,602,079 + Psyr_3006 0.72 -0.5 3,602,079 + Psyr_3006 0.72 +0.1 3,602,178 + +0.0 3,602,178 + -0.4 3,602,178 + +0.0 3,602,178 + -2.0 3,602,178 + +0.0 3,602,178 + -0.0 3,602,179 - -0.5 3,602,179 - -1.4 3,602,179 - +0.5 3,602,179 - +1.2 3,602,265 - Psyr_3007 0.20 +0.3 3,602,265 - Psyr_3007 0.20 -1.1 3,602,502 + Psyr_3007 0.75 -0.7
Or see this region's nucleotide sequence