Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2974 and Psyr_2975 are separated by 124 nucleotides Psyr_2975 and Psyr_2976 are separated by 110 nucleotides
Psyr_2974: Psyr_2974 - FAD-dependent pyridine nucleotide-disulfide oxidoreductase, at 3,566,107 to 3,567,669
_2974
Psyr_2975: Psyr_2975 - Alkyl hydroperoxide reductase/ Thiol specific antioxidant/ Mal allergen, at 3,567,794 to 3,568,357
_2975
Psyr_2976: Psyr_2976 - Phage integrase:Phage integrase, N-terminal SAM-like protein, at 3,568,468 to 3,569,427
_2976
Position (kb)
3567
3568
3569 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3566.883 kb on + strand, within Psyr_2974 at 3566.883 kb on + strand, within Psyr_2974 at 3566.985 kb on - strand, within Psyr_2974 at 3566.985 kb on - strand, within Psyr_2974 at 3567.149 kb on + strand, within Psyr_2974 at 3567.149 kb on + strand, within Psyr_2974 at 3567.149 kb on + strand, within Psyr_2974 at 3567.150 kb on - strand, within Psyr_2974 at 3567.241 kb on + strand, within Psyr_2974 at 3567.302 kb on + strand, within Psyr_2974 at 3567.489 kb on + strand, within Psyr_2974 at 3567.489 kb on + strand, within Psyr_2974 at 3567.490 kb on - strand, within Psyr_2974 at 3567.490 kb on - strand, within Psyr_2974 at 3567.686 kb on + strand at 3567.686 kb on + strand at 3567.695 kb on + strand at 3567.701 kb on + strand at 3567.702 kb on - strand at 3567.753 kb on - strand at 3567.832 kb on + strand at 3567.832 kb on + strand at 3567.833 kb on - strand at 3567.888 kb on + strand, within Psyr_2975 at 3567.889 kb on - strand, within Psyr_2975 at 3568.028 kb on + strand, within Psyr_2975 at 3568.028 kb on + strand, within Psyr_2975 at 3568.029 kb on - strand, within Psyr_2975 at 3568.078 kb on - strand, within Psyr_2975 at 3568.078 kb on - strand, within Psyr_2975 at 3568.078 kb on - strand, within Psyr_2975 at 3568.078 kb on - strand, within Psyr_2975 at 3568.078 kb on - strand, within Psyr_2975 at 3568.152 kb on + strand, within Psyr_2975 at 3568.152 kb on + strand, within Psyr_2975 at 3568.152 kb on + strand, within Psyr_2975 at 3568.152 kb on + strand, within Psyr_2975 at 3568.152 kb on + strand, within Psyr_2975 at 3568.152 kb on + strand, within Psyr_2975 at 3568.153 kb on - strand, within Psyr_2975 at 3568.153 kb on - strand, within Psyr_2975 at 3568.153 kb on - strand, within Psyr_2975 at 3568.153 kb on - strand, within Psyr_2975 at 3568.185 kb on + strand, within Psyr_2975 at 3568.185 kb on + strand, within Psyr_2975 at 3568.185 kb on + strand, within Psyr_2975 at 3568.185 kb on + strand, within Psyr_2975 at 3568.186 kb on - strand, within Psyr_2975 at 3568.220 kb on + strand, within Psyr_2975 at 3568.250 kb on + strand, within Psyr_2975 at 3568.251 kb on - strand, within Psyr_2975 at 3568.335 kb on - strand at 3568.335 kb on - strand at 3568.351 kb on + strand at 3568.361 kb on + strand at 3568.361 kb on + strand at 3568.361 kb on + strand at 3568.362 kb on - strand at 3568.362 kb on - strand at 3568.362 kb on - strand at 3568.362 kb on - strand at 3568.362 kb on - strand at 3568.363 kb on + strand at 3568.364 kb on - strand at 3568.364 kb on - strand at 3568.364 kb on - strand at 3568.410 kb on + strand at 3568.410 kb on + strand at 3568.411 kb on - strand at 3568.411 kb on - strand at 3568.482 kb on + strand at 3568.551 kb on - strand at 3568.551 kb on - strand at 3568.582 kb on + strand, within Psyr_2976 at 3568.582 kb on + strand, within Psyr_2976 at 3568.583 kb on - strand, within Psyr_2976 at 3568.583 kb on - strand, within Psyr_2976 at 3568.586 kb on + strand, within Psyr_2976 at 3568.586 kb on + strand, within Psyr_2976 at 3568.586 kb on + strand, within Psyr_2976 at 3568.586 kb on + strand, within Psyr_2976 at 3568.586 kb on + strand, within Psyr_2976 at 3568.586 kb on + strand, within Psyr_2976 at 3568.586 kb on + strand, within Psyr_2976 at 3568.586 kb on + strand, within Psyr_2976 at 3568.586 kb on + strand, within Psyr_2976 at 3568.586 kb on + strand, within Psyr_2976 at 3568.587 kb on - strand, within Psyr_2976 at 3568.587 kb on - strand, within Psyr_2976 at 3568.587 kb on - strand, within Psyr_2976 at 3568.587 kb on - strand, within Psyr_2976 at 3568.664 kb on + strand, within Psyr_2976 at 3568.664 kb on + strand, within Psyr_2976 at 3568.665 kb on - strand, within Psyr_2976 at 3568.747 kb on + strand, within Psyr_2976 at 3568.747 kb on + strand, within Psyr_2976 at 3568.748 kb on - strand, within Psyr_2976 at 3568.748 kb on - strand, within Psyr_2976 at 3568.748 kb on - strand, within Psyr_2976 at 3568.752 kb on + strand, within Psyr_2976 at 3568.816 kb on + strand, within Psyr_2976 at 3568.816 kb on + strand, within Psyr_2976 at 3568.816 kb on + strand, within Psyr_2976 at 3568.816 kb on + strand, within Psyr_2976 at 3568.816 kb on + strand, within Psyr_2976 at 3568.816 kb on + strand, within Psyr_2976 at 3568.816 kb on + strand, within Psyr_2976 at 3568.816 kb on + strand, within Psyr_2976 at 3568.816 kb on + strand, within Psyr_2976 at 3568.816 kb on + strand, within Psyr_2976 at 3568.816 kb on + strand, within Psyr_2976 at 3568.816 kb on + strand, within Psyr_2976 at 3568.816 kb on + strand, within Psyr_2976 at 3568.816 kb on + strand, within Psyr_2976 at 3568.817 kb on - strand, within Psyr_2976 at 3568.817 kb on - strand, within Psyr_2976 at 3568.817 kb on - strand, within Psyr_2976 at 3568.817 kb on - strand, within Psyr_2976 at 3568.817 kb on - strand, within Psyr_2976 at 3568.817 kb on - strand, within Psyr_2976 at 3568.817 kb on - strand, within Psyr_2976 at 3568.817 kb on - strand, within Psyr_2976 at 3568.817 kb on - strand, within Psyr_2976 at 3568.817 kb on - strand, within Psyr_2976 at 3568.817 kb on - strand, within Psyr_2976 at 3568.817 kb on - strand, within Psyr_2976 at 3568.817 kb on - strand, within Psyr_2976 at 3568.817 kb on - strand, within Psyr_2976 at 3568.818 kb on + strand, within Psyr_2976 at 3568.819 kb on - strand, within Psyr_2976 at 3568.819 kb on - strand, within Psyr_2976 at 3568.820 kb on + strand, within Psyr_2976 at 3568.820 kb on + strand, within Psyr_2976 at 3568.820 kb on + strand, within Psyr_2976 at 3568.820 kb on + strand, within Psyr_2976 at 3568.821 kb on - strand, within Psyr_2976 at 3568.821 kb on - strand, within Psyr_2976 at 3568.821 kb on - strand, within Psyr_2976 at 3568.821 kb on - strand, within Psyr_2976 at 3568.821 kb on - strand, within Psyr_2976 at 3568.821 kb on - strand, within Psyr_2976 at 3568.821 kb on - strand, within Psyr_2976 at 3568.821 kb on - strand, within Psyr_2976 at 3568.863 kb on - strand, within Psyr_2976 at 3568.907 kb on + strand, within Psyr_2976 at 3568.907 kb on + strand, within Psyr_2976 at 3568.907 kb on + strand, within Psyr_2976 at 3568.908 kb on - strand, within Psyr_2976 at 3568.908 kb on - strand, within Psyr_2976 at 3568.908 kb on - strand, within Psyr_2976 at 3568.908 kb on - strand, within Psyr_2976 at 3568.948 kb on + strand, within Psyr_2976 at 3568.949 kb on - strand, within Psyr_2976 at 3568.949 kb on - strand, within Psyr_2976 at 3569.111 kb on - strand, within Psyr_2976
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 3,566,883 + Psyr_2974 0.50 +0.6 3,566,883 + Psyr_2974 0.50 +0.0 3,566,985 - Psyr_2974 0.56 +0.0 3,566,985 - Psyr_2974 0.56 -2.4 3,567,149 + Psyr_2974 0.67 -0.5 3,567,149 + Psyr_2974 0.67 -0.4 3,567,149 + Psyr_2974 0.67 +0.6 3,567,150 - Psyr_2974 0.67 +1.2 3,567,241 + Psyr_2974 0.73 +0.1 3,567,302 + Psyr_2974 0.76 -2.6 3,567,489 + Psyr_2974 0.88 +1.9 3,567,489 + Psyr_2974 0.88 -0.2 3,567,490 - Psyr_2974 0.88 -1.3 3,567,490 - Psyr_2974 0.88 +0.6 3,567,686 + -2.4 3,567,686 + +1.2 3,567,695 + -1.2 3,567,701 + -2.2 3,567,702 - +0.2 3,567,753 - -1.7 3,567,832 + +0.6 3,567,832 + -1.0 3,567,833 - -0.0 3,567,888 + Psyr_2975 0.17 -1.2 3,567,889 - Psyr_2975 0.17 -2.2 3,568,028 + Psyr_2975 0.41 -0.2 3,568,028 + Psyr_2975 0.41 -0.9 3,568,029 - Psyr_2975 0.42 -0.7 3,568,078 - Psyr_2975 0.50 +1.2 3,568,078 - Psyr_2975 0.50 +0.2 3,568,078 - Psyr_2975 0.50 -0.7 3,568,078 - Psyr_2975 0.50 -1.0 3,568,078 - Psyr_2975 0.50 -0.1 3,568,152 + Psyr_2975 0.63 -1.0 3,568,152 + Psyr_2975 0.63 +0.4 3,568,152 + Psyr_2975 0.63 -0.4 3,568,152 + Psyr_2975 0.63 -0.8 3,568,152 + Psyr_2975 0.63 +1.2 3,568,152 + Psyr_2975 0.63 -0.7 3,568,153 - Psyr_2975 0.64 +1.2 3,568,153 - Psyr_2975 0.64 +0.6 3,568,153 - Psyr_2975 0.64 -0.1 3,568,153 - Psyr_2975 0.64 +0.7 3,568,185 + Psyr_2975 0.69 -1.7 3,568,185 + Psyr_2975 0.69 -1.0 3,568,185 + Psyr_2975 0.69 +0.6 3,568,185 + Psyr_2975 0.69 -1.7 3,568,186 - Psyr_2975 0.70 -0.9 3,568,220 + Psyr_2975 0.76 +0.2 3,568,250 + Psyr_2975 0.81 +0.6 3,568,251 - Psyr_2975 0.81 -0.4 3,568,335 - -0.3 3,568,335 - -0.6 3,568,351 + +0.8 3,568,361 + -1.7 3,568,361 + -0.9 3,568,361 + -0.9 3,568,362 - -1.0 3,568,362 - -0.3 3,568,362 - +0.8 3,568,362 - +0.4 3,568,362 - +0.4 3,568,363 + +2.6 3,568,364 - -0.8 3,568,364 - -0.4 3,568,364 - +0.1 3,568,410 + +0.4 3,568,410 + -0.1 3,568,411 - +0.6 3,568,411 - -1.0 3,568,482 + -0.4 3,568,551 - +1.9 3,568,551 - -1.1 3,568,582 + Psyr_2976 0.12 -2.0 3,568,582 + Psyr_2976 0.12 -0.3 3,568,583 - Psyr_2976 0.12 +0.6 3,568,583 - Psyr_2976 0.12 +0.2 3,568,586 + Psyr_2976 0.12 +0.0 3,568,586 + Psyr_2976 0.12 +0.8 3,568,586 + Psyr_2976 0.12 +0.0 3,568,586 + Psyr_2976 0.12 -1.7 3,568,586 + Psyr_2976 0.12 -1.0 3,568,586 + Psyr_2976 0.12 -0.6 3,568,586 + Psyr_2976 0.12 -1.5 3,568,586 + Psyr_2976 0.12 -0.1 3,568,586 + Psyr_2976 0.12 +0.5 3,568,586 + Psyr_2976 0.12 +0.8 3,568,587 - Psyr_2976 0.12 -0.9 3,568,587 - Psyr_2976 0.12 -1.4 3,568,587 - Psyr_2976 0.12 -1.2 3,568,587 - Psyr_2976 0.12 +1.6 3,568,664 + Psyr_2976 0.20 -1.7 3,568,664 + Psyr_2976 0.20 -1.0 3,568,665 - Psyr_2976 0.21 +0.4 3,568,747 + Psyr_2976 0.29 +0.2 3,568,747 + Psyr_2976 0.29 +0.6 3,568,748 - Psyr_2976 0.29 +0.2 3,568,748 - Psyr_2976 0.29 +1.2 3,568,748 - Psyr_2976 0.29 +0.2 3,568,752 + Psyr_2976 0.30 +1.2 3,568,816 + Psyr_2976 0.36 -0.4 3,568,816 + Psyr_2976 0.36 +0.9 3,568,816 + Psyr_2976 0.36 -0.2 3,568,816 + Psyr_2976 0.36 -1.7 3,568,816 + Psyr_2976 0.36 -0.4 3,568,816 + Psyr_2976 0.36 +0.2 3,568,816 + Psyr_2976 0.36 -0.9 3,568,816 + Psyr_2976 0.36 -0.3 3,568,816 + Psyr_2976 0.36 -0.5 3,568,816 + Psyr_2976 0.36 +0.2 3,568,816 + Psyr_2976 0.36 -0.5 3,568,816 + Psyr_2976 0.36 +1.1 3,568,816 + Psyr_2976 0.36 +2.9 3,568,816 + Psyr_2976 0.36 -0.7 3,568,817 - Psyr_2976 0.36 -3.5 3,568,817 - Psyr_2976 0.36 +0.6 3,568,817 - Psyr_2976 0.36 +0.1 3,568,817 - Psyr_2976 0.36 -0.2 3,568,817 - Psyr_2976 0.36 +0.0 3,568,817 - Psyr_2976 0.36 +1.5 3,568,817 - Psyr_2976 0.36 -1.0 3,568,817 - Psyr_2976 0.36 -0.8 3,568,817 - Psyr_2976 0.36 -1.0 3,568,817 - Psyr_2976 0.36 +0.4 3,568,817 - Psyr_2976 0.36 -1.7 3,568,817 - Psyr_2976 0.36 -1.7 3,568,817 - Psyr_2976 0.36 +0.9 3,568,817 - Psyr_2976 0.36 +0.6 3,568,818 + Psyr_2976 0.36 -1.2 3,568,819 - Psyr_2976 0.37 +1.9 3,568,819 - Psyr_2976 0.37 +0.1 3,568,820 + Psyr_2976 0.37 -0.6 3,568,820 + Psyr_2976 0.37 +1.0 3,568,820 + Psyr_2976 0.37 -1.7 3,568,820 + Psyr_2976 0.37 +0.4 3,568,821 - Psyr_2976 0.37 -1.0 3,568,821 - Psyr_2976 0.37 +1.2 3,568,821 - Psyr_2976 0.37 +0.6 3,568,821 - Psyr_2976 0.37 -1.7 3,568,821 - Psyr_2976 0.37 +0.1 3,568,821 - Psyr_2976 0.37 +1.6 3,568,821 - Psyr_2976 0.37 -0.1 3,568,821 - Psyr_2976 0.37 +0.7 3,568,863 - Psyr_2976 0.41 +0.2 3,568,907 + Psyr_2976 0.46 -1.7 3,568,907 + Psyr_2976 0.46 -0.6 3,568,907 + Psyr_2976 0.46 -0.5 3,568,908 - Psyr_2976 0.46 -1.4 3,568,908 - Psyr_2976 0.46 +0.6 3,568,908 - Psyr_2976 0.46 +2.9 3,568,908 - Psyr_2976 0.46 -1.8 3,568,948 + Psyr_2976 0.50 -0.4 3,568,949 - Psyr_2976 0.50 -0.4 3,568,949 - Psyr_2976 0.50 -0.4 3,569,111 - Psyr_2976 0.67 -1.0
Or see this region's nucleotide sequence