Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2753 and Psyr_2754 are separated by 49 nucleotides Psyr_2754 and Psyr_2755 are separated by 135 nucleotides Psyr_2755 and Psyr_2756 are separated by 101 nucleotides Psyr_2756 and Psyr_2757 are separated by 0 nucleotides
Psyr_2753: Psyr_2753 - Zinc-containing alcohol dehydrogenase superfamily, at 3,341,164 to 3,342,147
_2753
Psyr_2754: Psyr_2754 - regulatory protein, TetR, at 3,342,197 to 3,342,856
_2754
Psyr_2755: Psyr_2755 - 2-nitropropane dioxygenase, NPD, at 3,342,992 to 3,344,071
_2755
Psyr_2756: Psyr_2756 - Molybdenum ABC transporter, periplasmic binding protein, at 3,344,173 to 3,344,940
_2756
Psyr_2757: Psyr_2757 - Binding-protein-dependent transport systems inner membrane component, at 3,344,941 to 3,345,621
_2757
Position (kb)
3342
3343
3344
3345 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3342.023 kb on + strand, within Psyr_2753 at 3342.023 kb on + strand, within Psyr_2753 at 3342.023 kb on + strand, within Psyr_2753 at 3342.023 kb on + strand, within Psyr_2753 at 3342.023 kb on + strand, within Psyr_2753 at 3342.023 kb on + strand, within Psyr_2753 at 3342.024 kb on - strand, within Psyr_2753 at 3342.024 kb on - strand, within Psyr_2753 at 3342.024 kb on - strand, within Psyr_2753 at 3342.024 kb on - strand, within Psyr_2753 at 3342.024 kb on - strand, within Psyr_2753 at 3342.024 kb on - strand, within Psyr_2753 at 3342.147 kb on + strand at 3342.147 kb on + strand at 3342.148 kb on - strand at 3342.148 kb on - strand at 3342.148 kb on - strand at 3342.148 kb on - strand at 3342.148 kb on - strand at 3342.247 kb on + strand at 3342.248 kb on - strand at 3342.601 kb on - strand, within Psyr_2754 at 3342.601 kb on - strand, within Psyr_2754 at 3342.645 kb on + strand, within Psyr_2754 at 3342.645 kb on + strand, within Psyr_2754 at 3342.645 kb on + strand, within Psyr_2754 at 3342.645 kb on + strand, within Psyr_2754 at 3342.645 kb on + strand, within Psyr_2754 at 3342.645 kb on + strand, within Psyr_2754 at 3342.651 kb on + strand, within Psyr_2754 at 3342.652 kb on - strand, within Psyr_2754 at 3342.652 kb on - strand, within Psyr_2754 at 3342.652 kb on - strand, within Psyr_2754 at 3342.652 kb on - strand, within Psyr_2754 at 3342.690 kb on + strand, within Psyr_2754 at 3342.690 kb on + strand, within Psyr_2754 at 3342.690 kb on + strand, within Psyr_2754 at 3342.690 kb on + strand, within Psyr_2754 at 3342.690 kb on + strand at 3342.690 kb on + strand, within Psyr_2754 at 3342.691 kb on - strand, within Psyr_2754 at 3342.691 kb on - strand, within Psyr_2754 at 3342.691 kb on - strand, within Psyr_2754 at 3342.691 kb on - strand, within Psyr_2754 at 3342.691 kb on - strand, within Psyr_2754 at 3342.691 kb on - strand, within Psyr_2754 at 3342.691 kb on - strand, within Psyr_2754 at 3342.691 kb on - strand, within Psyr_2754 at 3342.691 kb on - strand, within Psyr_2754 at 3342.856 kb on + strand at 3342.868 kb on - strand at 3343.283 kb on + strand, within Psyr_2755 at 3343.286 kb on + strand, within Psyr_2755 at 3343.286 kb on + strand, within Psyr_2755 at 3343.287 kb on - strand, within Psyr_2755 at 3343.678 kb on - strand, within Psyr_2755 at 3343.691 kb on - strand, within Psyr_2755 at 3343.727 kb on + strand, within Psyr_2755 at 3343.728 kb on - strand, within Psyr_2755 at 3343.728 kb on - strand, within Psyr_2755 at 3343.728 kb on - strand, within Psyr_2755 at 3344.102 kb on + strand at 3344.103 kb on - strand at 3344.104 kb on + strand at 3344.105 kb on - strand at 3344.106 kb on + strand at 3344.106 kb on + strand at 3344.106 kb on + strand at 3344.106 kb on + strand at 3344.106 kb on + strand at 3344.107 kb on - strand at 3344.107 kb on - strand at 3344.111 kb on - strand at 3344.114 kb on + strand at 3344.115 kb on - strand at 3344.115 kb on - strand at 3344.115 kb on - strand at 3344.117 kb on - strand at 3344.184 kb on + strand at 3344.374 kb on + strand, within Psyr_2756 at 3344.374 kb on + strand, within Psyr_2756 at 3344.374 kb on + strand, within Psyr_2756 at 3344.374 kb on + strand, within Psyr_2756 at 3344.374 kb on + strand, within Psyr_2756 at 3344.375 kb on - strand, within Psyr_2756 at 3344.375 kb on - strand, within Psyr_2756 at 3344.375 kb on - strand, within Psyr_2756 at 3344.375 kb on - strand, within Psyr_2756 at 3344.481 kb on + strand, within Psyr_2756 at 3344.486 kb on - strand, within Psyr_2756 at 3344.527 kb on + strand, within Psyr_2756 at 3344.527 kb on + strand, within Psyr_2756 at 3344.527 kb on + strand, within Psyr_2756 at 3344.527 kb on + strand, within Psyr_2756 at 3344.527 kb on + strand, within Psyr_2756 at 3344.528 kb on - strand, within Psyr_2756 at 3344.528 kb on - strand, within Psyr_2756 at 3344.528 kb on - strand, within Psyr_2756 at 3344.528 kb on - strand, within Psyr_2756 at 3344.528 kb on - strand, within Psyr_2756 at 3344.528 kb on - strand, within Psyr_2756 at 3344.528 kb on - strand, within Psyr_2756 at 3344.550 kb on - strand, within Psyr_2756 at 3344.550 kb on - strand, within Psyr_2756 at 3344.688 kb on + strand, within Psyr_2756 at 3344.755 kb on + strand, within Psyr_2756 at 3344.755 kb on + strand, within Psyr_2756 at 3344.755 kb on + strand, within Psyr_2756 at 3344.756 kb on - strand, within Psyr_2756 at 3344.756 kb on - strand, within Psyr_2756 at 3344.756 kb on - strand, within Psyr_2756 at 3344.756 kb on - strand, within Psyr_2756 at 3344.756 kb on - strand, within Psyr_2756 at 3344.938 kb on + strand at 3344.938 kb on + strand at 3344.939 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 3,342,023 + Psyr_2753 0.87 -0.5 3,342,023 + Psyr_2753 0.87 +0.5 3,342,023 + Psyr_2753 0.87 -0.1 3,342,023 + Psyr_2753 0.87 +0.3 3,342,023 + Psyr_2753 0.87 -0.3 3,342,023 + Psyr_2753 0.87 +1.5 3,342,024 - Psyr_2753 0.87 -0.3 3,342,024 - Psyr_2753 0.87 -0.7 3,342,024 - Psyr_2753 0.87 +0.1 3,342,024 - Psyr_2753 0.87 +0.3 3,342,024 - Psyr_2753 0.87 +0.6 3,342,024 - Psyr_2753 0.87 +0.2 3,342,147 + +0.6 3,342,147 + -0.7 3,342,148 - +1.9 3,342,148 - +1.0 3,342,148 - -1.4 3,342,148 - -0.1 3,342,148 - -0.3 3,342,247 + +1.2 3,342,248 - -0.8 3,342,601 - Psyr_2754 0.61 -0.5 3,342,601 - Psyr_2754 0.61 -0.1 3,342,645 + Psyr_2754 0.68 +0.3 3,342,645 + Psyr_2754 0.68 -0.1 3,342,645 + Psyr_2754 0.68 +0.6 3,342,645 + Psyr_2754 0.68 +0.5 3,342,645 + Psyr_2754 0.68 +1.6 3,342,645 + Psyr_2754 0.68 +0.3 3,342,651 + Psyr_2754 0.69 -0.1 3,342,652 - Psyr_2754 0.69 +0.5 3,342,652 - Psyr_2754 0.69 -0.9 3,342,652 - Psyr_2754 0.69 +0.8 3,342,652 - Psyr_2754 0.69 -0.4 3,342,690 + Psyr_2754 0.75 +1.9 3,342,690 + Psyr_2754 0.75 -0.9 3,342,690 + Psyr_2754 0.75 +1.6 3,342,690 + Psyr_2754 0.75 -0.2 3,342,690 + -0.4 3,342,690 + Psyr_2754 0.75 +0.3 3,342,691 - Psyr_2754 0.75 -0.2 3,342,691 - Psyr_2754 0.75 -0.5 3,342,691 - Psyr_2754 0.75 -0.0 3,342,691 - Psyr_2754 0.75 -0.9 3,342,691 - Psyr_2754 0.75 -1.0 3,342,691 - Psyr_2754 0.75 +0.3 3,342,691 - Psyr_2754 0.75 -0.1 3,342,691 - Psyr_2754 0.75 +1.5 3,342,691 - Psyr_2754 0.75 +0.1 3,342,856 + -1.1 3,342,868 - +1.8 3,343,283 + Psyr_2755 0.27 -0.9 3,343,286 + Psyr_2755 0.27 -1.0 3,343,286 + Psyr_2755 0.27 -1.0 3,343,287 - Psyr_2755 0.27 -1.2 3,343,678 - Psyr_2755 0.64 -0.4 3,343,691 - Psyr_2755 0.65 -1.8 3,343,727 + Psyr_2755 0.68 +1.2 3,343,728 - Psyr_2755 0.68 -2.1 3,343,728 - Psyr_2755 0.68 -0.3 3,343,728 - Psyr_2755 0.68 -1.2 3,344,102 + +0.0 3,344,103 - -0.0 3,344,104 + +1.1 3,344,105 - -0.1 3,344,106 + -1.0 3,344,106 + +0.8 3,344,106 + -1.2 3,344,106 + +0.3 3,344,106 + -1.8 3,344,107 - +0.8 3,344,107 - -1.1 3,344,111 - -1.2 3,344,114 + -1.1 3,344,115 - -0.3 3,344,115 - +0.3 3,344,115 - -0.7 3,344,117 - -0.8 3,344,184 + +0.1 3,344,374 + Psyr_2756 0.26 +1.0 3,344,374 + Psyr_2756 0.26 -1.4 3,344,374 + Psyr_2756 0.26 +2.7 3,344,374 + Psyr_2756 0.26 +0.0 3,344,374 + Psyr_2756 0.26 -0.5 3,344,375 - Psyr_2756 0.26 -0.1 3,344,375 - Psyr_2756 0.26 -0.1 3,344,375 - Psyr_2756 0.26 -0.2 3,344,375 - Psyr_2756 0.26 -0.7 3,344,481 + Psyr_2756 0.40 +0.1 3,344,486 - Psyr_2756 0.41 +0.6 3,344,527 + Psyr_2756 0.46 +0.7 3,344,527 + Psyr_2756 0.46 +0.7 3,344,527 + Psyr_2756 0.46 -0.9 3,344,527 + Psyr_2756 0.46 -1.1 3,344,527 + Psyr_2756 0.46 -0.5 3,344,528 - Psyr_2756 0.46 +1.9 3,344,528 - Psyr_2756 0.46 +0.5 3,344,528 - Psyr_2756 0.46 -0.4 3,344,528 - Psyr_2756 0.46 -0.2 3,344,528 - Psyr_2756 0.46 -1.4 3,344,528 - Psyr_2756 0.46 -0.5 3,344,528 - Psyr_2756 0.46 +0.3 3,344,550 - Psyr_2756 0.49 -1.2 3,344,550 - Psyr_2756 0.49 +0.1 3,344,688 + Psyr_2756 0.67 +0.4 3,344,755 + Psyr_2756 0.76 -3.1 3,344,755 + Psyr_2756 0.76 +1.0 3,344,755 + Psyr_2756 0.76 -0.5 3,344,756 - Psyr_2756 0.76 +3.3 3,344,756 - Psyr_2756 0.76 +1.1 3,344,756 - Psyr_2756 0.76 -0.8 3,344,756 - Psyr_2756 0.76 -0.1 3,344,756 - Psyr_2756 0.76 -0.2 3,344,938 + -2.1 3,344,938 + -0.4 3,344,939 - +0.9
Or see this region's nucleotide sequence