Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2617 and Psyr_2618 are separated by 2 nucleotides Psyr_2618 and Psyr_2619 are separated by 214 nucleotides
Psyr_2617: Psyr_2617 - Secretion protein HlyD, at 3,172,404 to 3,173,561
_2617
Psyr_2618: Psyr_2618 - ABC transporter:Protein of unknown function DUF214, at 3,173,564 to 3,175,525
_2618
Psyr_2619: Psyr_2619 - diaminobutyrate aminotransferase apoenzyme, at 3,175,740 to 3,177,074
_2619
Position (kb)
3173
3174
3175
3176 Strain fitness (log2 ratio)
-2
-1
0
1 at 3172.785 kb on + strand, within Psyr_2617 at 3172.786 kb on - strand, within Psyr_2617 at 3172.947 kb on + strand, within Psyr_2617 at 3173.131 kb on - strand, within Psyr_2617 at 3173.759 kb on + strand at 3173.759 kb on + strand at 3173.900 kb on - strand, within Psyr_2618 at 3173.900 kb on - strand, within Psyr_2618 at 3174.509 kb on - strand, within Psyr_2618 at 3174.605 kb on + strand, within Psyr_2618 at 3174.606 kb on - strand, within Psyr_2618 at 3174.606 kb on - strand, within Psyr_2618 at 3174.608 kb on + strand, within Psyr_2618 at 3174.608 kb on + strand, within Psyr_2618 at 3174.608 kb on + strand, within Psyr_2618 at 3174.609 kb on - strand, within Psyr_2618 at 3175.091 kb on + strand, within Psyr_2618 at 3175.091 kb on + strand, within Psyr_2618 at 3175.091 kb on + strand, within Psyr_2618 at 3175.092 kb on - strand, within Psyr_2618 at 3175.365 kb on - strand at 3175.631 kb on + strand at 3175.632 kb on - strand at 3175.634 kb on + strand at 3175.634 kb on + strand at 3175.634 kb on + strand at 3175.635 kb on - strand at 3175.635 kb on - strand at 3175.635 kb on - strand at 3175.779 kb on - strand at 3176.118 kb on - strand, within Psyr_2619 at 3176.199 kb on - strand, within Psyr_2619 at 3176.490 kb on + strand, within Psyr_2619 at 3176.503 kb on + strand, within Psyr_2619 at 3176.503 kb on + strand, within Psyr_2619 at 3176.525 kb on + strand, within Psyr_2619
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 3,172,785 + Psyr_2617 0.33 +0.9 3,172,786 - Psyr_2617 0.33 -1.9 3,172,947 + Psyr_2617 0.47 +0.2 3,173,131 - Psyr_2617 0.63 -0.5 3,173,759 + +1.3 3,173,759 + -0.9 3,173,900 - Psyr_2618 0.17 +1.3 3,173,900 - Psyr_2618 0.17 +0.2 3,174,509 - Psyr_2618 0.48 +0.2 3,174,605 + Psyr_2618 0.53 +0.2 3,174,606 - Psyr_2618 0.53 +0.3 3,174,606 - Psyr_2618 0.53 -0.4 3,174,608 + Psyr_2618 0.53 -0.4 3,174,608 + Psyr_2618 0.53 -1.3 3,174,608 + Psyr_2618 0.53 -2.5 3,174,609 - Psyr_2618 0.53 -0.8 3,175,091 + Psyr_2618 0.78 +0.6 3,175,091 + Psyr_2618 0.78 +0.7 3,175,091 + Psyr_2618 0.78 +0.3 3,175,092 - Psyr_2618 0.78 -0.6 3,175,365 - +1.2 3,175,631 + +0.7 3,175,632 - -0.5 3,175,634 + -1.0 3,175,634 + +1.4 3,175,634 + -0.8 3,175,635 - -1.2 3,175,635 - +0.6 3,175,635 - +0.6 3,175,779 - -0.1 3,176,118 - Psyr_2619 0.28 -1.0 3,176,199 - Psyr_2619 0.34 -0.3 3,176,490 + Psyr_2619 0.56 +0.3 3,176,503 + Psyr_2619 0.57 +1.4 3,176,503 + Psyr_2619 0.57 -0.8 3,176,525 + Psyr_2619 0.59 +0.4
Or see this region's nucleotide sequence