Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2480 and Psyr_2481 are separated by 90 nucleotides Psyr_2481 and Psyr_2482 are separated by 153 nucleotides Psyr_2482 and Psyr_2483 overlap by 4 nucleotides
Psyr_2480: Psyr_2480 - transcriptional regulator, LysR family, at 2,877,813 to 2,878,730
_2480
Psyr_2481: Psyr_2481 - thiol peroxidase (atypical 2-Cys peroxiredoxin), at 2,878,821 to 2,879,321
_2481
Psyr_2482: Psyr_2482 - Secretion protein HlyD, at 2,879,475 to 2,880,779
_2482
Psyr_2483: Psyr_2483 - Acriflavin resistance protein, at 2,880,776 to 2,883,886
_2483
Position (kb)
2879
2880
2881 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2878.495 kb on - strand, within Psyr_2480 at 2878.495 kb on - strand, within Psyr_2480 at 2878.706 kb on + strand at 2878.754 kb on + strand at 2878.754 kb on + strand at 2878.754 kb on + strand at 2878.755 kb on - strand at 2878.755 kb on - strand at 2878.964 kb on - strand, within Psyr_2481 at 2878.988 kb on + strand, within Psyr_2481 at 2879.011 kb on + strand, within Psyr_2481 at 2879.015 kb on + strand, within Psyr_2481 at 2879.016 kb on - strand, within Psyr_2481 at 2879.016 kb on - strand, within Psyr_2481 at 2879.016 kb on - strand, within Psyr_2481 at 2879.169 kb on + strand, within Psyr_2481 at 2879.170 kb on - strand, within Psyr_2481 at 2879.170 kb on - strand, within Psyr_2481 at 2879.292 kb on + strand at 2879.293 kb on - strand at 2879.293 kb on - strand at 2879.319 kb on + strand at 2879.320 kb on - strand at 2879.334 kb on + strand at 2879.334 kb on + strand at 2879.335 kb on - strand at 2879.335 kb on - strand at 2879.335 kb on - strand at 2879.335 kb on - strand at 2879.335 kb on - strand at 2879.336 kb on + strand at 2879.382 kb on + strand at 2879.425 kb on + strand at 2879.425 kb on + strand at 2879.430 kb on + strand at 2879.430 kb on + strand at 2879.430 kb on + strand at 2879.431 kb on - strand at 2879.431 kb on - strand at 2879.431 kb on - strand at 2879.440 kb on + strand at 2879.441 kb on - strand at 2879.485 kb on + strand at 2879.485 kb on + strand at 2879.485 kb on + strand at 2879.718 kb on + strand, within Psyr_2482 at 2879.718 kb on + strand, within Psyr_2482 at 2879.719 kb on - strand, within Psyr_2482 at 2879.719 kb on - strand, within Psyr_2482 at 2879.719 kb on - strand, within Psyr_2482 at 2879.719 kb on - strand, within Psyr_2482 at 2879.722 kb on - strand, within Psyr_2482 at 2879.799 kb on + strand, within Psyr_2482 at 2879.800 kb on - strand, within Psyr_2482 at 2879.891 kb on + strand, within Psyr_2482 at 2880.018 kb on + strand, within Psyr_2482 at 2880.019 kb on - strand, within Psyr_2482 at 2880.425 kb on + strand, within Psyr_2482 at 2880.427 kb on + strand, within Psyr_2482 at 2880.427 kb on + strand, within Psyr_2482 at 2880.428 kb on - strand, within Psyr_2482 at 2880.428 kb on - strand, within Psyr_2482 at 2880.428 kb on - strand, within Psyr_2482 at 2880.511 kb on - strand, within Psyr_2482 at 2880.775 kb on + strand at 2880.775 kb on + strand at 2880.775 kb on + strand at 2880.775 kb on - strand at 2880.776 kb on - strand at 2880.776 kb on - strand at 2880.776 kb on - strand at 2880.776 kb on - strand at 2880.860 kb on + strand at 2880.862 kb on + strand at 2880.863 kb on - strand at 2881.048 kb on + strand at 2881.048 kb on + strand at 2881.160 kb on + strand, within Psyr_2483 at 2881.259 kb on - strand, within Psyr_2483 at 2881.496 kb on + strand, within Psyr_2483
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 2,878,495 - Psyr_2480 0.74 +0.5 2,878,495 - Psyr_2480 0.74 +0.9 2,878,706 + -0.9 2,878,754 + +0.8 2,878,754 + -0.8 2,878,754 + -0.1 2,878,755 - -0.9 2,878,755 - -0.5 2,878,964 - Psyr_2481 0.29 +1.1 2,878,988 + Psyr_2481 0.33 -1.5 2,879,011 + Psyr_2481 0.38 +0.1 2,879,015 + Psyr_2481 0.39 -0.3 2,879,016 - Psyr_2481 0.39 -1.7 2,879,016 - Psyr_2481 0.39 +0.9 2,879,016 - Psyr_2481 0.39 +1.9 2,879,169 + Psyr_2481 0.69 -0.0 2,879,170 - Psyr_2481 0.70 -0.9 2,879,170 - Psyr_2481 0.70 +2.8 2,879,292 + +0.8 2,879,293 - +1.3 2,879,293 - +0.5 2,879,319 + -1.0 2,879,320 - -0.3 2,879,334 + +0.1 2,879,334 + -2.2 2,879,335 - -1.5 2,879,335 - +2.1 2,879,335 - -0.5 2,879,335 - -1.0 2,879,335 - -1.2 2,879,336 + -0.7 2,879,382 + +0.1 2,879,425 + -0.5 2,879,425 + -1.0 2,879,430 + -0.5 2,879,430 + +0.1 2,879,430 + -1.5 2,879,431 - +1.5 2,879,431 - +0.2 2,879,431 - -0.6 2,879,440 + +1.9 2,879,441 - -0.5 2,879,485 + -0.5 2,879,485 + -1.2 2,879,485 + -0.9 2,879,718 + Psyr_2482 0.19 -0.7 2,879,718 + Psyr_2482 0.19 -0.1 2,879,719 - Psyr_2482 0.19 -1.8 2,879,719 - Psyr_2482 0.19 -1.3 2,879,719 - Psyr_2482 0.19 -0.5 2,879,719 - Psyr_2482 0.19 +0.3 2,879,722 - Psyr_2482 0.19 -1.0 2,879,799 + Psyr_2482 0.25 -1.1 2,879,800 - Psyr_2482 0.25 -0.5 2,879,891 + Psyr_2482 0.32 -1.1 2,880,018 + Psyr_2482 0.42 -0.5 2,880,019 - Psyr_2482 0.42 -0.8 2,880,425 + Psyr_2482 0.73 -0.5 2,880,427 + Psyr_2482 0.73 +0.0 2,880,427 + Psyr_2482 0.73 -0.6 2,880,428 - Psyr_2482 0.73 +1.0 2,880,428 - Psyr_2482 0.73 +1.3 2,880,428 - Psyr_2482 0.73 -0.5 2,880,511 - Psyr_2482 0.79 -1.3 2,880,775 + +1.1 2,880,775 + +0.2 2,880,775 + -0.4 2,880,775 - -0.5 2,880,776 - -0.8 2,880,776 - +0.3 2,880,776 - +0.5 2,880,776 - +0.4 2,880,860 + -0.7 2,880,862 + -0.2 2,880,863 - -0.5 2,881,048 + -0.5 2,881,048 + -1.0 2,881,160 + Psyr_2483 0.12 -1.6 2,881,259 - Psyr_2483 0.16 +0.1 2,881,496 + Psyr_2483 0.23 -2.5
Or see this region's nucleotide sequence