Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2471 and Psyr_2472 are separated by 30 nucleotides Psyr_2472 and Psyr_2473 are separated by 165 nucleotides Psyr_2473 and Psyr_2474 are separated by 185 nucleotides
Psyr_2471: Psyr_2471 - hydroxymethylglutaryl-CoA lyase, at 2,865,211 to 2,866,110
_2471
Psyr_2472: Psyr_2472 - transcriptional regulator, MerR family, at 2,866,141 to 2,866,533
_2472
Psyr_2473: Psyr_2473 - transcriptional regulator, LysR family, at 2,866,699 to 2,867,628
_2473
Psyr_2474: Psyr_2474 - Acyl-CoA dehydrogenase, C-terminal:Acyl-CoA dehydrogenase, central region, at 2,867,814 to 2,869,043
_2474
Position (kb)
2866
2867
2868 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2866.125 kb on - strand at 2866.125 kb on - strand at 2866.316 kb on + strand, within Psyr_2472 at 2866.317 kb on - strand, within Psyr_2472 at 2866.409 kb on + strand, within Psyr_2472 at 2866.410 kb on - strand, within Psyr_2472 at 2866.410 kb on - strand, within Psyr_2472 at 2866.410 kb on - strand, within Psyr_2472 at 2866.410 kb on - strand, within Psyr_2472 at 2866.410 kb on - strand, within Psyr_2472 at 2866.410 kb on - strand, within Psyr_2472 at 2866.413 kb on + strand, within Psyr_2472 at 2866.414 kb on - strand, within Psyr_2472 at 2866.414 kb on - strand, within Psyr_2472 at 2866.414 kb on - strand, within Psyr_2472 at 2866.414 kb on - strand, within Psyr_2472 at 2866.414 kb on - strand, within Psyr_2472 at 2866.414 kb on - strand, within Psyr_2472 at 2866.414 kb on - strand, within Psyr_2472 at 2866.414 kb on - strand, within Psyr_2472 at 2866.414 kb on - strand, within Psyr_2472 at 2866.414 kb on - strand, within Psyr_2472 at 2866.518 kb on - strand at 2866.518 kb on - strand at 2866.552 kb on - strand at 2866.552 kb on - strand at 2866.553 kb on + strand at 2866.554 kb on - strand at 2866.554 kb on - strand at 2866.554 kb on - strand at 2866.554 kb on - strand at 2866.554 kb on - strand at 2866.554 kb on - strand at 2866.554 kb on - strand at 2866.850 kb on + strand, within Psyr_2473 at 2866.850 kb on + strand, within Psyr_2473 at 2866.856 kb on + strand, within Psyr_2473 at 2866.856 kb on + strand, within Psyr_2473 at 2866.856 kb on + strand, within Psyr_2473 at 2866.856 kb on + strand, within Psyr_2473 at 2866.856 kb on + strand, within Psyr_2473 at 2866.857 kb on - strand, within Psyr_2473 at 2866.857 kb on - strand, within Psyr_2473 at 2866.857 kb on - strand, within Psyr_2473 at 2866.857 kb on - strand, within Psyr_2473 at 2866.857 kb on - strand, within Psyr_2473 at 2866.857 kb on - strand, within Psyr_2473 at 2866.940 kb on + strand, within Psyr_2473 at 2866.940 kb on + strand, within Psyr_2473 at 2866.940 kb on + strand, within Psyr_2473 at 2866.940 kb on + strand, within Psyr_2473 at 2866.940 kb on + strand, within Psyr_2473 at 2866.940 kb on + strand, within Psyr_2473 at 2866.940 kb on + strand, within Psyr_2473 at 2866.940 kb on + strand, within Psyr_2473 at 2866.940 kb on + strand, within Psyr_2473 at 2866.941 kb on - strand, within Psyr_2473 at 2866.941 kb on - strand, within Psyr_2473 at 2866.941 kb on - strand, within Psyr_2473 at 2866.941 kb on - strand, within Psyr_2473 at 2866.941 kb on - strand, within Psyr_2473 at 2867.031 kb on + strand, within Psyr_2473 at 2867.031 kb on + strand, within Psyr_2473 at 2867.037 kb on + strand, within Psyr_2473 at 2867.038 kb on - strand, within Psyr_2473 at 2867.118 kb on - strand, within Psyr_2473 at 2867.576 kb on + strand at 2867.577 kb on - strand at 2867.577 kb on - strand at 2867.667 kb on + strand at 2867.702 kb on + strand at 2867.702 kb on + strand at 2867.702 kb on + strand at 2867.702 kb on + strand at 2867.703 kb on - strand at 2867.724 kb on - strand at 2867.741 kb on + strand at 2867.741 kb on + strand at 2867.742 kb on - strand at 2867.742 kb on - strand at 2867.764 kb on + strand at 2867.765 kb on - strand at 2867.880 kb on + strand at 2867.880 kb on + strand at 2867.880 kb on + strand at 2867.881 kb on - strand at 2867.881 kb on - strand at 2867.881 kb on - strand at 2868.045 kb on + strand, within Psyr_2474 at 2868.046 kb on - strand, within Psyr_2474
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 2,866,125 - -1.8 2,866,125 - -1.2 2,866,316 + Psyr_2472 0.45 -0.0 2,866,317 - Psyr_2472 0.45 -0.8 2,866,409 + Psyr_2472 0.68 -0.4 2,866,410 - Psyr_2472 0.68 -0.4 2,866,410 - Psyr_2472 0.68 -0.5 2,866,410 - Psyr_2472 0.68 +1.0 2,866,410 - Psyr_2472 0.68 -1.0 2,866,410 - Psyr_2472 0.68 -0.9 2,866,410 - Psyr_2472 0.68 -0.1 2,866,413 + Psyr_2472 0.69 -1.0 2,866,414 - Psyr_2472 0.69 +0.2 2,866,414 - Psyr_2472 0.69 +0.8 2,866,414 - Psyr_2472 0.69 -0.9 2,866,414 - Psyr_2472 0.69 -1.3 2,866,414 - Psyr_2472 0.69 -0.4 2,866,414 - Psyr_2472 0.69 -0.3 2,866,414 - Psyr_2472 0.69 +0.7 2,866,414 - Psyr_2472 0.69 -1.8 2,866,414 - Psyr_2472 0.69 -1.3 2,866,414 - Psyr_2472 0.69 -0.5 2,866,518 - -0.1 2,866,518 - -1.0 2,866,552 - -0.2 2,866,552 - -0.4 2,866,553 + -0.8 2,866,554 - -0.3 2,866,554 - -1.3 2,866,554 - -0.0 2,866,554 - +1.8 2,866,554 - -0.2 2,866,554 - -1.8 2,866,554 - -0.8 2,866,850 + Psyr_2473 0.16 +0.8 2,866,850 + Psyr_2473 0.16 +0.2 2,866,856 + Psyr_2473 0.17 -1.0 2,866,856 + Psyr_2473 0.17 -1.8 2,866,856 + Psyr_2473 0.17 -2.2 2,866,856 + Psyr_2473 0.17 -0.4 2,866,856 + Psyr_2473 0.17 -0.9 2,866,857 - Psyr_2473 0.17 -0.9 2,866,857 - Psyr_2473 0.17 -0.7 2,866,857 - Psyr_2473 0.17 -0.0 2,866,857 - Psyr_2473 0.17 -0.0 2,866,857 - Psyr_2473 0.17 -2.2 2,866,857 - Psyr_2473 0.17 -1.8 2,866,940 + Psyr_2473 0.26 -0.4 2,866,940 + Psyr_2473 0.26 -0.7 2,866,940 + Psyr_2473 0.26 -1.3 2,866,940 + Psyr_2473 0.26 -0.5 2,866,940 + Psyr_2473 0.26 -0.6 2,866,940 + Psyr_2473 0.26 -0.4 2,866,940 + Psyr_2473 0.26 +1.6 2,866,940 + Psyr_2473 0.26 +0.7 2,866,940 + Psyr_2473 0.26 -0.4 2,866,941 - Psyr_2473 0.26 -1.3 2,866,941 - Psyr_2473 0.26 +0.9 2,866,941 - Psyr_2473 0.26 -0.4 2,866,941 - Psyr_2473 0.26 -0.2 2,866,941 - Psyr_2473 0.26 +0.2 2,867,031 + Psyr_2473 0.36 -1.0 2,867,031 + Psyr_2473 0.36 -0.8 2,867,037 + Psyr_2473 0.36 +1.4 2,867,038 - Psyr_2473 0.36 +0.6 2,867,118 - Psyr_2473 0.45 +0.0 2,867,576 + +1.9 2,867,577 - -0.4 2,867,577 - -1.4 2,867,667 + +0.3 2,867,702 + +0.6 2,867,702 + +0.1 2,867,702 + -1.3 2,867,702 + +0.3 2,867,703 - -0.7 2,867,724 - -1.4 2,867,741 + +0.1 2,867,741 + +1.0 2,867,742 - -2.2 2,867,742 - -0.2 2,867,764 + -1.4 2,867,765 - +1.1 2,867,880 + +0.0 2,867,880 + +0.7 2,867,880 + -1.3 2,867,881 - -2.3 2,867,881 - +1.3 2,867,881 - -0.6 2,868,045 + Psyr_2474 0.19 -0.2 2,868,046 - Psyr_2474 0.19 -0.1
Or see this region's nucleotide sequence