Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2364 and Psyr_2365 overlap by 4 nucleotides Psyr_2365 and Psyr_2366 are separated by 356 nucleotides
Psyr_2364: Psyr_2364 - conserved hypothetical protein, at 2,741,814 to 2,742,653
_2364
Psyr_2365: Psyr_2365 - Radical SAM, at 2,742,650 to 2,743,870
_2365
Psyr_2366: Psyr_2366 - ABC transporter, at 2,744,227 to 2,744,973
_2366
Position (kb)
2742
2743
2744 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2741.677 kb on + strand at 2741.677 kb on + strand at 2741.698 kb on - strand at 2741.698 kb on - strand at 2741.716 kb on + strand at 2741.801 kb on + strand at 2741.801 kb on + strand at 2741.801 kb on + strand at 2741.802 kb on - strand at 2741.802 kb on - strand at 2741.802 kb on - strand at 2741.802 kb on - strand at 2742.010 kb on + strand, within Psyr_2364 at 2742.010 kb on + strand, within Psyr_2364 at 2742.013 kb on + strand, within Psyr_2364 at 2742.014 kb on - strand, within Psyr_2364 at 2742.112 kb on + strand, within Psyr_2364 at 2742.113 kb on - strand, within Psyr_2364 at 2742.115 kb on + strand, within Psyr_2364 at 2742.116 kb on - strand, within Psyr_2364 at 2742.116 kb on - strand, within Psyr_2364 at 2742.116 kb on - strand, within Psyr_2364 at 2742.226 kb on + strand, within Psyr_2364 at 2742.409 kb on + strand, within Psyr_2364 at 2743.015 kb on - strand, within Psyr_2365 at 2743.041 kb on + strand, within Psyr_2365 at 2743.041 kb on + strand, within Psyr_2365 at 2743.041 kb on + strand, within Psyr_2365 at 2743.041 kb on + strand, within Psyr_2365 at 2743.042 kb on - strand, within Psyr_2365 at 2743.042 kb on - strand, within Psyr_2365 at 2743.122 kb on + strand, within Psyr_2365 at 2743.122 kb on + strand, within Psyr_2365 at 2743.122 kb on + strand, within Psyr_2365 at 2743.122 kb on + strand, within Psyr_2365 at 2743.123 kb on - strand, within Psyr_2365 at 2743.123 kb on - strand, within Psyr_2365 at 2743.123 kb on - strand, within Psyr_2365 at 2743.123 kb on - strand, within Psyr_2365 at 2743.125 kb on + strand, within Psyr_2365 at 2743.126 kb on - strand, within Psyr_2365 at 2743.152 kb on + strand, within Psyr_2365 at 2743.152 kb on + strand, within Psyr_2365 at 2743.284 kb on + strand, within Psyr_2365 at 2743.284 kb on + strand, within Psyr_2365 at 2743.284 kb on + strand, within Psyr_2365 at 2743.452 kb on + strand, within Psyr_2365 at 2743.453 kb on - strand, within Psyr_2365 at 2743.516 kb on - strand, within Psyr_2365 at 2743.575 kb on + strand, within Psyr_2365 at 2743.657 kb on - strand, within Psyr_2365 at 2743.726 kb on + strand, within Psyr_2365 at 2743.726 kb on + strand, within Psyr_2365 at 2743.727 kb on - strand, within Psyr_2365 at 2743.728 kb on + strand, within Psyr_2365 at 2743.728 kb on + strand, within Psyr_2365 at 2743.728 kb on + strand, within Psyr_2365 at 2743.728 kb on + strand, within Psyr_2365 at 2743.728 kb on + strand, within Psyr_2365 at 2743.728 kb on + strand, within Psyr_2365 at 2743.729 kb on - strand, within Psyr_2365 at 2743.729 kb on - strand, within Psyr_2365 at 2743.729 kb on - strand, within Psyr_2365 at 2743.729 kb on - strand, within Psyr_2365 at 2743.729 kb on - strand, within Psyr_2365 at 2743.729 kb on - strand, within Psyr_2365 at 2743.805 kb on - strand at 2743.824 kb on + strand at 2743.825 kb on - strand at 2743.825 kb on - strand at 2743.825 kb on - strand at 2743.825 kb on - strand at 2743.861 kb on + strand at 2743.892 kb on + strand at 2743.893 kb on - strand at 2743.981 kb on + strand at 2743.981 kb on + strand at 2743.982 kb on - strand at 2744.076 kb on - strand at 2744.076 kb on - strand at 2744.081 kb on + strand at 2744.188 kb on - strand at 2744.285 kb on + strand at 2744.285 kb on + strand at 2744.286 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 2,741,677 + -0.6 2,741,677 + -0.5 2,741,698 - -0.2 2,741,698 - -1.0 2,741,716 + +0.8 2,741,801 + +0.3 2,741,801 + +0.6 2,741,801 + -1.0 2,741,802 - -0.6 2,741,802 - -0.2 2,741,802 - -0.0 2,741,802 - -1.7 2,742,010 + Psyr_2364 0.23 +0.8 2,742,010 + Psyr_2364 0.23 +0.8 2,742,013 + Psyr_2364 0.24 -1.1 2,742,014 - Psyr_2364 0.24 -0.4 2,742,112 + Psyr_2364 0.35 -1.2 2,742,113 - Psyr_2364 0.36 +0.6 2,742,115 + Psyr_2364 0.36 +0.5 2,742,116 - Psyr_2364 0.36 +0.6 2,742,116 - Psyr_2364 0.36 +0.8 2,742,116 - Psyr_2364 0.36 -0.0 2,742,226 + Psyr_2364 0.49 -0.1 2,742,409 + Psyr_2364 0.71 +1.6 2,743,015 - Psyr_2365 0.30 +0.8 2,743,041 + Psyr_2365 0.32 +0.6 2,743,041 + Psyr_2365 0.32 +1.0 2,743,041 + Psyr_2365 0.32 +0.6 2,743,041 + Psyr_2365 0.32 +1.0 2,743,042 - Psyr_2365 0.32 +0.6 2,743,042 - Psyr_2365 0.32 -0.4 2,743,122 + Psyr_2365 0.39 +0.4 2,743,122 + Psyr_2365 0.39 +0.2 2,743,122 + Psyr_2365 0.39 -0.4 2,743,122 + Psyr_2365 0.39 +1.6 2,743,123 - Psyr_2365 0.39 -0.2 2,743,123 - Psyr_2365 0.39 +1.0 2,743,123 - Psyr_2365 0.39 -0.3 2,743,123 - Psyr_2365 0.39 -0.1 2,743,125 + Psyr_2365 0.39 -0.2 2,743,126 - Psyr_2365 0.39 +0.5 2,743,152 + Psyr_2365 0.41 +1.2 2,743,152 + Psyr_2365 0.41 +0.5 2,743,284 + Psyr_2365 0.52 +0.1 2,743,284 + Psyr_2365 0.52 +1.2 2,743,284 + Psyr_2365 0.52 -0.2 2,743,452 + Psyr_2365 0.66 +0.0 2,743,453 - Psyr_2365 0.66 +0.5 2,743,516 - Psyr_2365 0.71 -0.5 2,743,575 + Psyr_2365 0.76 -0.5 2,743,657 - Psyr_2365 0.82 -0.2 2,743,726 + Psyr_2365 0.88 +0.8 2,743,726 + Psyr_2365 0.88 -0.2 2,743,727 - Psyr_2365 0.88 -0.3 2,743,728 + Psyr_2365 0.88 -1.2 2,743,728 + Psyr_2365 0.88 -0.0 2,743,728 + Psyr_2365 0.88 -2.4 2,743,728 + Psyr_2365 0.88 +1.0 2,743,728 + Psyr_2365 0.88 -0.0 2,743,728 + Psyr_2365 0.88 +0.1 2,743,729 - Psyr_2365 0.88 +0.8 2,743,729 - Psyr_2365 0.88 -2.5 2,743,729 - Psyr_2365 0.88 -1.5 2,743,729 - Psyr_2365 0.88 -0.6 2,743,729 - Psyr_2365 0.88 +0.0 2,743,729 - Psyr_2365 0.88 -0.0 2,743,805 - +0.0 2,743,824 + +0.6 2,743,825 - +1.6 2,743,825 - +0.6 2,743,825 - -0.6 2,743,825 - -0.2 2,743,861 + -0.5 2,743,892 + -0.2 2,743,893 - -1.0 2,743,981 + +0.4 2,743,981 + +0.4 2,743,982 - -0.2 2,744,076 - +2.2 2,744,076 - +0.2 2,744,081 + +0.9 2,744,188 - +0.2 2,744,285 + -1.0 2,744,285 + -1.1 2,744,286 - -1.4
Or see this region's nucleotide sequence