Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1999 and Psyr_2000 are separated by 217 nucleotides Psyr_2000 and Psyr_2001 are separated by 114 nucleotides Psyr_2001 and Psyr_2002 are separated by 94 nucleotides
Psyr_1999: Psyr_1999 - OmpA/MotB, at 2,320,206 to 2,321,021
_1999
Psyr_2000: Psyr_2000 - transcriptional regulator, GntR family, at 2,321,239 to 2,322,678
_2000
Psyr_2001: Psyr_2001 - conserved hypothetical protein, at 2,322,793 to 2,323,434
_2001
Psyr_2002: Psyr_2002 - Protein of unknown function UPF0153, at 2,323,529 to 2,323,786
_2002
Position (kb)
2321
2322
2323 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2320.261 kb on + strand at 2320.261 kb on + strand at 2320.263 kb on + strand at 2320.340 kb on + strand, within Psyr_1999 at 2320.420 kb on - strand, within Psyr_1999 at 2320.450 kb on - strand, within Psyr_1999 at 2320.455 kb on + strand, within Psyr_1999 at 2320.550 kb on + strand, within Psyr_1999 at 2320.789 kb on - strand, within Psyr_1999 at 2320.790 kb on + strand, within Psyr_1999 at 2320.791 kb on - strand, within Psyr_1999 at 2320.892 kb on + strand, within Psyr_1999 at 2321.023 kb on - strand at 2321.185 kb on + strand at 2321.195 kb on + strand at 2321.195 kb on + strand at 2321.195 kb on + strand at 2321.195 kb on + strand at 2321.196 kb on - strand at 2321.196 kb on - strand at 2321.196 kb on - strand at 2321.196 kb on - strand at 2321.257 kb on + strand at 2321.306 kb on - strand at 2321.375 kb on + strand at 2321.375 kb on + strand at 2321.375 kb on + strand at 2321.375 kb on + strand at 2321.375 kb on + strand at 2321.376 kb on - strand at 2321.440 kb on + strand, within Psyr_2000 at 2321.440 kb on + strand, within Psyr_2000 at 2321.440 kb on + strand, within Psyr_2000 at 2321.440 kb on + strand, within Psyr_2000 at 2321.440 kb on + strand, within Psyr_2000 at 2321.440 kb on + strand, within Psyr_2000 at 2321.440 kb on + strand, within Psyr_2000 at 2321.441 kb on - strand, within Psyr_2000 at 2321.441 kb on - strand, within Psyr_2000 at 2321.600 kb on - strand, within Psyr_2000 at 2321.600 kb on - strand, within Psyr_2000 at 2321.671 kb on + strand, within Psyr_2000 at 2321.671 kb on + strand, within Psyr_2000 at 2321.671 kb on + strand, within Psyr_2000 at 2321.671 kb on + strand, within Psyr_2000 at 2321.671 kb on + strand, within Psyr_2000 at 2321.671 kb on + strand, within Psyr_2000 at 2321.671 kb on + strand, within Psyr_2000 at 2321.671 kb on + strand, within Psyr_2000 at 2321.671 kb on + strand, within Psyr_2000 at 2321.671 kb on + strand, within Psyr_2000 at 2321.671 kb on + strand, within Psyr_2000 at 2321.672 kb on - strand, within Psyr_2000 at 2321.672 kb on - strand, within Psyr_2000 at 2321.672 kb on - strand, within Psyr_2000 at 2321.672 kb on - strand, within Psyr_2000 at 2321.672 kb on - strand, within Psyr_2000 at 2321.672 kb on - strand, within Psyr_2000 at 2321.672 kb on - strand, within Psyr_2000 at 2321.672 kb on - strand, within Psyr_2000 at 2321.672 kb on - strand, within Psyr_2000 at 2321.672 kb on - strand, within Psyr_2000 at 2321.672 kb on - strand, within Psyr_2000 at 2321.672 kb on - strand, within Psyr_2000 at 2321.721 kb on + strand, within Psyr_2000 at 2322.094 kb on + strand, within Psyr_2000 at 2322.095 kb on - strand, within Psyr_2000 at 2322.095 kb on - strand, within Psyr_2000 at 2322.116 kb on + strand, within Psyr_2000 at 2322.116 kb on + strand, within Psyr_2000 at 2322.166 kb on + strand, within Psyr_2000 at 2322.167 kb on - strand, within Psyr_2000 at 2322.167 kb on - strand, within Psyr_2000 at 2322.167 kb on - strand, within Psyr_2000 at 2322.232 kb on + strand, within Psyr_2000 at 2322.382 kb on + strand, within Psyr_2000 at 2322.382 kb on + strand, within Psyr_2000 at 2322.383 kb on - strand, within Psyr_2000 at 2322.570 kb on + strand at 2322.570 kb on + strand at 2322.570 kb on + strand at 2322.571 kb on - strand at 2322.571 kb on - strand at 2322.571 kb on - strand at 2322.571 kb on - strand at 2322.650 kb on + strand at 2322.650 kb on + strand at 2322.650 kb on + strand at 2322.650 kb on + strand at 2322.650 kb on + strand at 2322.651 kb on - strand at 2322.674 kb on - strand at 2322.792 kb on + strand at 2322.831 kb on - strand at 2323.257 kb on + strand, within Psyr_2001 at 2323.377 kb on + strand at 2323.377 kb on + strand at 2323.397 kb on + strand at 2323.397 kb on + strand at 2323.397 kb on + strand at 2323.397 kb on + strand at 2323.397 kb on + strand at 2323.397 kb on + strand at 2323.398 kb on - strand at 2323.398 kb on - strand at 2323.398 kb on - strand at 2323.398 kb on - strand at 2323.432 kb on + strand at 2323.432 kb on + strand at 2323.432 kb on + strand at 2323.432 kb on + strand at 2323.433 kb on - strand at 2323.433 kb on - strand at 2323.433 kb on - strand at 2323.433 kb on - strand at 2323.433 kb on - strand at 2323.433 kb on - strand at 2323.563 kb on + strand, within Psyr_2002
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 2,320,261 + +0.2 2,320,261 + -0.9 2,320,263 + -1.2 2,320,340 + Psyr_1999 0.16 +1.5 2,320,420 - Psyr_1999 0.26 -0.0 2,320,450 - Psyr_1999 0.30 -0.4 2,320,455 + Psyr_1999 0.31 +0.7 2,320,550 + Psyr_1999 0.42 +1.0 2,320,789 - Psyr_1999 0.71 +0.2 2,320,790 + Psyr_1999 0.72 -0.2 2,320,791 - Psyr_1999 0.72 -1.9 2,320,892 + Psyr_1999 0.84 +1.5 2,321,023 - +0.7 2,321,185 + -0.6 2,321,195 + -0.9 2,321,195 + -0.0 2,321,195 + +2.3 2,321,195 + -0.9 2,321,196 - +0.6 2,321,196 - -0.8 2,321,196 - -0.6 2,321,196 - -0.0 2,321,257 + +1.0 2,321,306 - -0.0 2,321,375 + -0.2 2,321,375 + +0.6 2,321,375 + -0.6 2,321,375 + -0.1 2,321,375 + +1.4 2,321,376 - -1.4 2,321,440 + Psyr_2000 0.14 -0.0 2,321,440 + Psyr_2000 0.14 +0.5 2,321,440 + Psyr_2000 0.14 +0.2 2,321,440 + Psyr_2000 0.14 -1.4 2,321,440 + Psyr_2000 0.14 -2.0 2,321,440 + Psyr_2000 0.14 -2.4 2,321,440 + Psyr_2000 0.14 -0.6 2,321,441 - Psyr_2000 0.14 +0.2 2,321,441 - Psyr_2000 0.14 +0.5 2,321,600 - Psyr_2000 0.25 -1.4 2,321,600 - Psyr_2000 0.25 -1.0 2,321,671 + Psyr_2000 0.30 -1.0 2,321,671 + Psyr_2000 0.30 -0.0 2,321,671 + Psyr_2000 0.30 -2.6 2,321,671 + Psyr_2000 0.30 -0.0 2,321,671 + Psyr_2000 0.30 -0.8 2,321,671 + Psyr_2000 0.30 +0.7 2,321,671 + Psyr_2000 0.30 -0.8 2,321,671 + Psyr_2000 0.30 +2.0 2,321,671 + Psyr_2000 0.30 -1.0 2,321,671 + Psyr_2000 0.30 +0.4 2,321,671 + Psyr_2000 0.30 -2.4 2,321,672 - Psyr_2000 0.30 +0.2 2,321,672 - Psyr_2000 0.30 +1.8 2,321,672 - Psyr_2000 0.30 +0.6 2,321,672 - Psyr_2000 0.30 -0.6 2,321,672 - Psyr_2000 0.30 -1.4 2,321,672 - Psyr_2000 0.30 +1.2 2,321,672 - Psyr_2000 0.30 -0.9 2,321,672 - Psyr_2000 0.30 -0.1 2,321,672 - Psyr_2000 0.30 -0.5 2,321,672 - Psyr_2000 0.30 -0.8 2,321,672 - Psyr_2000 0.30 +1.4 2,321,672 - Psyr_2000 0.30 -0.0 2,321,721 + Psyr_2000 0.33 +0.2 2,322,094 + Psyr_2000 0.59 -1.6 2,322,095 - Psyr_2000 0.59 +0.2 2,322,095 - Psyr_2000 0.59 +0.3 2,322,116 + Psyr_2000 0.61 +1.0 2,322,116 + Psyr_2000 0.61 +0.5 2,322,166 + Psyr_2000 0.64 -0.4 2,322,167 - Psyr_2000 0.64 -2.5 2,322,167 - Psyr_2000 0.64 +0.4 2,322,167 - Psyr_2000 0.64 +1.5 2,322,232 + Psyr_2000 0.69 -0.6 2,322,382 + Psyr_2000 0.79 -0.2 2,322,382 + Psyr_2000 0.79 -0.4 2,322,383 - Psyr_2000 0.79 +0.4 2,322,570 + -0.0 2,322,570 + -0.1 2,322,570 + +0.4 2,322,571 - -0.5 2,322,571 - -1.0 2,322,571 - -0.6 2,322,571 - -0.6 2,322,650 + -0.8 2,322,650 + +0.4 2,322,650 + +1.0 2,322,650 + +0.1 2,322,650 + +2.0 2,322,651 - -0.2 2,322,674 - -0.2 2,322,792 + -2.0 2,322,831 - +2.3 2,323,257 + Psyr_2001 0.72 -1.8 2,323,377 + -0.0 2,323,377 + -0.4 2,323,397 + +0.2 2,323,397 + -0.8 2,323,397 + +1.0 2,323,397 + -1.0 2,323,397 + -0.2 2,323,397 + -1.0 2,323,398 - -2.4 2,323,398 - -0.0 2,323,398 - -0.4 2,323,398 - +0.5 2,323,432 + -0.6 2,323,432 + -0.4 2,323,432 + -2.0 2,323,432 + -0.4 2,323,433 - +0.1 2,323,433 - -0.9 2,323,433 - +0.2 2,323,433 - -0.9 2,323,433 - +0.6 2,323,433 - -1.5 2,323,563 + Psyr_2002 0.13 -0.0
Or see this region's nucleotide sequence