Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1996 and Psyr_1997 are separated by 116 nucleotides Psyr_1997 and Psyr_1998 are separated by 10 nucleotides Psyr_1998 and Psyr_1999 overlap by 4 nucleotides
Psyr_1996: Psyr_1996 - Electron transfer flavoprotein, alpha subunit, at 2,317,972 to 2,318,901
_1996
Psyr_1997: Psyr_1997 - amino acid ABC transporter substrate-binding protein, PAAT family, at 2,319,018 to 2,319,845
_1997
Psyr_1998: Psyr_1998 - lipoprotein, putative, at 2,319,856 to 2,320,209
_1998
Psyr_1999: Psyr_1999 - OmpA/MotB, at 2,320,206 to 2,321,021
_1999
Position (kb)
2319
2320
2321 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2318.917 kb on + strand at 2318.918 kb on - strand at 2318.918 kb on - strand at 2318.918 kb on - strand at 2318.919 kb on + strand at 2318.920 kb on - strand at 2318.995 kb on - strand at 2319.001 kb on + strand at 2319.139 kb on - strand, within Psyr_1997 at 2319.342 kb on + strand, within Psyr_1997 at 2319.343 kb on - strand, within Psyr_1997 at 2319.567 kb on + strand, within Psyr_1997 at 2319.567 kb on + strand, within Psyr_1997 at 2319.568 kb on - strand, within Psyr_1997 at 2319.568 kb on - strand, within Psyr_1997 at 2319.568 kb on - strand, within Psyr_1997 at 2319.568 kb on - strand, within Psyr_1997 at 2319.600 kb on - strand, within Psyr_1997 at 2319.613 kb on + strand, within Psyr_1997 at 2319.614 kb on - strand, within Psyr_1997 at 2319.843 kb on + strand at 2319.843 kb on + strand at 2319.844 kb on - strand at 2320.060 kb on + strand, within Psyr_1998 at 2320.061 kb on - strand, within Psyr_1998 at 2320.061 kb on - strand, within Psyr_1998 at 2320.061 kb on - strand, within Psyr_1998 at 2320.214 kb on + strand at 2320.261 kb on + strand at 2320.261 kb on + strand at 2320.263 kb on + strand at 2320.340 kb on + strand, within Psyr_1999 at 2320.420 kb on - strand, within Psyr_1999 at 2320.450 kb on - strand, within Psyr_1999 at 2320.455 kb on + strand, within Psyr_1999 at 2320.550 kb on + strand, within Psyr_1999 at 2320.789 kb on - strand, within Psyr_1999 at 2320.790 kb on + strand, within Psyr_1999 at 2320.791 kb on - strand, within Psyr_1999 at 2320.892 kb on + strand, within Psyr_1999 at 2321.023 kb on - strand at 2321.185 kb on + strand at 2321.195 kb on + strand at 2321.195 kb on + strand at 2321.195 kb on + strand at 2321.195 kb on + strand at 2321.196 kb on - strand at 2321.196 kb on - strand at 2321.196 kb on - strand at 2321.196 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 2,318,917 + -0.6 2,318,918 - -1.5 2,318,918 - -0.3 2,318,918 - -0.0 2,318,919 + -0.0 2,318,920 - +0.5 2,318,995 - +0.4 2,319,001 + +0.6 2,319,139 - Psyr_1997 0.15 -1.3 2,319,342 + Psyr_1997 0.39 -1.0 2,319,343 - Psyr_1997 0.39 -0.9 2,319,567 + Psyr_1997 0.66 -1.2 2,319,567 + Psyr_1997 0.66 -1.3 2,319,568 - Psyr_1997 0.66 -0.0 2,319,568 - Psyr_1997 0.66 +0.4 2,319,568 - Psyr_1997 0.66 +2.3 2,319,568 - Psyr_1997 0.66 +0.6 2,319,600 - Psyr_1997 0.70 +0.2 2,319,613 + Psyr_1997 0.72 +1.2 2,319,614 - Psyr_1997 0.72 -0.6 2,319,843 + -0.1 2,319,843 + +0.4 2,319,844 - -0.4 2,320,060 + Psyr_1998 0.58 +0.6 2,320,061 - Psyr_1998 0.58 +0.7 2,320,061 - Psyr_1998 0.58 -1.2 2,320,061 - Psyr_1998 0.58 +1.0 2,320,214 + -0.8 2,320,261 + +0.2 2,320,261 + -0.9 2,320,263 + -1.2 2,320,340 + Psyr_1999 0.16 +1.5 2,320,420 - Psyr_1999 0.26 -0.0 2,320,450 - Psyr_1999 0.30 -0.4 2,320,455 + Psyr_1999 0.31 +0.7 2,320,550 + Psyr_1999 0.42 +1.0 2,320,789 - Psyr_1999 0.71 +0.2 2,320,790 + Psyr_1999 0.72 -0.2 2,320,791 - Psyr_1999 0.72 -1.9 2,320,892 + Psyr_1999 0.84 +1.5 2,321,023 - +0.7 2,321,185 + -0.6 2,321,195 + -0.9 2,321,195 + -0.0 2,321,195 + +2.3 2,321,195 + -0.9 2,321,196 - +0.6 2,321,196 - -0.8 2,321,196 - -0.6 2,321,196 - -0.0
Or see this region's nucleotide sequence