Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1978 and Psyr_1979 are separated by 29 nucleotides Psyr_1979 and Psyr_1980 are separated by 129 nucleotides Psyr_1980 and Psyr_1981 are separated by 67 nucleotides
Psyr_1978: Psyr_1978 - Thioesterase superfamily, at 2,297,353 to 2,297,814
_1978
Psyr_1979: Psyr_1979 - tRNA-U20a,U20b-dihydrouridine synthase, at 2,297,844 to 2,298,809
_1979
Psyr_1980: Psyr_1980 - Heat shock protein Hsp20, at 2,298,939 to 2,299,382
_1980
Psyr_1981: Psyr_1981 - PAS:GGDEF, at 2,299,450 to 2,301,642
_1981
Position (kb)
2297
2298
2299 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2296.891 kb on + strand at 2296.922 kb on + strand at 2296.927 kb on - strand at 2296.927 kb on - strand at 2297.003 kb on - strand at 2297.003 kb on - strand at 2297.003 kb on - strand at 2297.060 kb on + strand at 2297.062 kb on + strand at 2297.062 kb on + strand at 2297.062 kb on + strand at 2297.062 kb on + strand at 2297.062 kb on + strand at 2297.062 kb on + strand at 2297.062 kb on + strand at 2297.062 kb on + strand at 2297.062 kb on + strand at 2297.062 kb on + strand at 2297.062 kb on + strand at 2297.062 kb on + strand at 2297.062 kb on + strand at 2297.062 kb on + strand at 2297.062 kb on + strand at 2297.063 kb on - strand at 2297.063 kb on - strand at 2297.063 kb on - strand at 2297.063 kb on - strand at 2297.063 kb on - strand at 2297.063 kb on - strand at 2297.063 kb on - strand at 2297.063 kb on - strand at 2297.063 kb on - strand at 2297.063 kb on - strand at 2297.063 kb on - strand at 2297.063 kb on - strand at 2297.063 kb on - strand at 2297.063 kb on - strand at 2297.063 kb on - strand at 2297.063 kb on - strand at 2297.063 kb on - strand at 2297.063 kb on - strand at 2297.063 kb on - strand at 2297.063 kb on - strand at 2297.066 kb on + strand at 2297.066 kb on + strand at 2297.066 kb on + strand at 2297.066 kb on + strand at 2297.066 kb on + strand at 2297.066 kb on + strand at 2297.066 kb on + strand at 2297.067 kb on - strand at 2297.067 kb on - strand at 2297.067 kb on - strand at 2297.067 kb on - strand at 2297.067 kb on - strand at 2297.067 kb on - strand at 2297.067 kb on - strand at 2297.067 kb on - strand at 2297.067 kb on - strand at 2297.067 kb on - strand at 2297.067 kb on - strand at 2297.067 kb on - strand at 2297.067 kb on - strand at 2297.067 kb on - strand at 2297.067 kb on - strand at 2297.103 kb on + strand at 2297.103 kb on + strand at 2297.103 kb on + strand at 2297.103 kb on + strand at 2297.103 kb on + strand at 2297.104 kb on - strand at 2297.104 kb on - strand at 2297.104 kb on - strand at 2297.104 kb on - strand at 2297.104 kb on - strand at 2297.183 kb on - strand at 2297.241 kb on - strand at 2297.241 kb on - strand at 2297.334 kb on + strand at 2297.334 kb on + strand at 2297.334 kb on + strand at 2297.335 kb on - strand at 2297.335 kb on - strand at 2297.335 kb on - strand at 2297.335 kb on - strand at 2297.335 kb on - strand at 2297.335 kb on - strand at 2297.443 kb on + strand, within Psyr_1978 at 2297.443 kb on + strand, within Psyr_1978 at 2297.443 kb on + strand, within Psyr_1978 at 2297.443 kb on + strand, within Psyr_1978 at 2297.443 kb on + strand, within Psyr_1978 at 2297.444 kb on - strand, within Psyr_1978 at 2297.444 kb on - strand, within Psyr_1978 at 2297.444 kb on - strand, within Psyr_1978 at 2297.444 kb on - strand, within Psyr_1978 at 2297.444 kb on - strand, within Psyr_1978 at 2297.444 kb on - strand, within Psyr_1978 at 2297.444 kb on - strand, within Psyr_1978 at 2297.444 kb on - strand, within Psyr_1978 at 2297.444 kb on - strand, within Psyr_1978 at 2297.444 kb on - strand, within Psyr_1978 at 2297.444 kb on - strand, within Psyr_1978 at 2297.444 kb on - strand, within Psyr_1978 at 2297.762 kb on - strand, within Psyr_1978 at 2297.812 kb on + strand at 2297.977 kb on - strand, within Psyr_1979 at 2297.977 kb on - strand, within Psyr_1979 at 2297.977 kb on - strand, within Psyr_1979 at 2297.982 kb on + strand, within Psyr_1979 at 2297.982 kb on + strand, within Psyr_1979 at 2297.982 kb on + strand, within Psyr_1979 at 2297.982 kb on + strand, within Psyr_1979 at 2297.982 kb on + strand, within Psyr_1979 at 2297.982 kb on + strand, within Psyr_1979 at 2297.983 kb on - strand, within Psyr_1979 at 2297.983 kb on - strand, within Psyr_1979 at 2297.983 kb on - strand, within Psyr_1979 at 2297.983 kb on - strand, within Psyr_1979 at 2297.983 kb on - strand, within Psyr_1979 at 2297.988 kb on + strand, within Psyr_1979 at 2297.988 kb on + strand, within Psyr_1979 at 2297.988 kb on + strand, within Psyr_1979 at 2297.989 kb on - strand, within Psyr_1979 at 2297.989 kb on - strand, within Psyr_1979 at 2297.989 kb on - strand, within Psyr_1979 at 2297.989 kb on - strand, within Psyr_1979 at 2297.989 kb on - strand, within Psyr_1979 at 2298.029 kb on + strand, within Psyr_1979 at 2298.244 kb on + strand, within Psyr_1979 at 2298.369 kb on + strand, within Psyr_1979 at 2298.369 kb on + strand, within Psyr_1979 at 2298.731 kb on + strand at 2298.731 kb on + strand at 2298.731 kb on + strand at 2298.732 kb on - strand at 2298.732 kb on - strand at 2298.769 kb on + strand at 2298.770 kb on - strand at 2298.770 kb on - strand at 2298.870 kb on + strand at 2298.871 kb on - strand at 2298.908 kb on - strand at 2298.944 kb on + strand at 2298.945 kb on - strand at 2299.053 kb on + strand, within Psyr_1980 at 2299.053 kb on + strand, within Psyr_1980 at 2299.054 kb on - strand, within Psyr_1980 at 2299.200 kb on + strand, within Psyr_1980 at 2299.201 kb on - strand, within Psyr_1980 at 2299.201 kb on - strand, within Psyr_1980 at 2299.201 kb on - strand, within Psyr_1980 at 2299.259 kb on + strand, within Psyr_1980 at 2299.413 kb on + strand at 2299.414 kb on - strand at 2299.538 kb on + strand at 2299.547 kb on + strand at 2299.547 kb on + strand at 2299.547 kb on - strand at 2299.548 kb on - strand at 2299.548 kb on - strand at 2299.548 kb on - strand at 2299.553 kb on + strand at 2299.554 kb on - strand at 2299.554 kb on - strand at 2299.742 kb on + strand, within Psyr_1981 at 2299.742 kb on + strand, within Psyr_1981 at 2299.742 kb on + strand, within Psyr_1981 at 2299.742 kb on + strand, within Psyr_1981 at 2299.742 kb on + strand, within Psyr_1981 at 2299.743 kb on - strand, within Psyr_1981 at 2299.743 kb on - strand, within Psyr_1981
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 2,296,891 + -0.9 2,296,922 + -0.4 2,296,927 - +0.4 2,296,927 - +0.6 2,297,003 - +0.3 2,297,003 - -1.8 2,297,003 - -1.0 2,297,060 + -0.4 2,297,062 + -0.6 2,297,062 + -1.8 2,297,062 + -1.0 2,297,062 + +1.4 2,297,062 + -0.0 2,297,062 + -0.6 2,297,062 + -1.3 2,297,062 + +1.0 2,297,062 + -0.5 2,297,062 + -0.3 2,297,062 + -1.1 2,297,062 + +0.4 2,297,062 + +0.1 2,297,062 + -0.8 2,297,062 + +0.4 2,297,063 - +0.4 2,297,063 - +0.8 2,297,063 - -0.4 2,297,063 - -2.3 2,297,063 - -0.0 2,297,063 - +0.7 2,297,063 - -0.8 2,297,063 - -0.0 2,297,063 - +0.6 2,297,063 - -1.9 2,297,063 - +2.2 2,297,063 - -0.9 2,297,063 - -0.0 2,297,063 - -0.5 2,297,063 - -1.1 2,297,063 - -0.3 2,297,063 - -0.9 2,297,063 - -0.3 2,297,063 - -0.4 2,297,063 - -0.5 2,297,066 + +0.5 2,297,066 + -0.3 2,297,066 + +0.1 2,297,066 + -0.4 2,297,066 + -0.2 2,297,066 + -0.7 2,297,066 + -1.2 2,297,067 - +0.5 2,297,067 - -0.0 2,297,067 - -0.6 2,297,067 - -0.3 2,297,067 - +1.8 2,297,067 - -1.9 2,297,067 - +0.0 2,297,067 - +2.5 2,297,067 - -2.4 2,297,067 - +0.4 2,297,067 - -0.9 2,297,067 - +0.6 2,297,067 - -0.3 2,297,067 - +0.5 2,297,067 - +1.0 2,297,103 + +0.5 2,297,103 + -1.7 2,297,103 + -0.0 2,297,103 + -0.4 2,297,103 + +0.6 2,297,104 - -0.2 2,297,104 - -0.5 2,297,104 - +0.5 2,297,104 - -0.1 2,297,104 - -2.5 2,297,183 - +0.1 2,297,241 - +0.6 2,297,241 - -1.7 2,297,334 + +1.0 2,297,334 + +0.3 2,297,334 + -1.1 2,297,335 - +2.0 2,297,335 - -2.4 2,297,335 - +1.0 2,297,335 - +0.2 2,297,335 - +1.3 2,297,335 - -1.6 2,297,443 + Psyr_1978 0.19 -2.2 2,297,443 + Psyr_1978 0.19 -0.0 2,297,443 + Psyr_1978 0.19 +1.0 2,297,443 + Psyr_1978 0.19 +1.4 2,297,443 + Psyr_1978 0.19 -0.9 2,297,444 - Psyr_1978 0.20 +0.3 2,297,444 - Psyr_1978 0.20 -1.0 2,297,444 - Psyr_1978 0.20 -0.1 2,297,444 - Psyr_1978 0.20 -1.1 2,297,444 - Psyr_1978 0.20 +0.6 2,297,444 - Psyr_1978 0.20 +0.3 2,297,444 - Psyr_1978 0.20 -1.1 2,297,444 - Psyr_1978 0.20 -0.5 2,297,444 - Psyr_1978 0.20 -0.4 2,297,444 - Psyr_1978 0.20 +0.1 2,297,444 - Psyr_1978 0.20 -1.5 2,297,444 - Psyr_1978 0.20 -0.6 2,297,762 - Psyr_1978 0.89 -1.0 2,297,812 + -0.3 2,297,977 - Psyr_1979 0.14 +2.5 2,297,977 - Psyr_1979 0.14 -1.1 2,297,977 - Psyr_1979 0.14 -0.1 2,297,982 + Psyr_1979 0.14 -1.4 2,297,982 + Psyr_1979 0.14 -1.5 2,297,982 + Psyr_1979 0.14 -0.6 2,297,982 + Psyr_1979 0.14 -0.6 2,297,982 + Psyr_1979 0.14 -0.9 2,297,982 + Psyr_1979 0.14 -0.1 2,297,983 - Psyr_1979 0.14 -1.2 2,297,983 - Psyr_1979 0.14 +0.3 2,297,983 - Psyr_1979 0.14 -2.8 2,297,983 - Psyr_1979 0.14 +0.1 2,297,983 - Psyr_1979 0.14 +0.1 2,297,988 + Psyr_1979 0.15 -1.1 2,297,988 + Psyr_1979 0.15 -0.6 2,297,988 + Psyr_1979 0.15 -2.6 2,297,989 - Psyr_1979 0.15 -2.6 2,297,989 - Psyr_1979 0.15 -0.6 2,297,989 - Psyr_1979 0.15 -0.8 2,297,989 - Psyr_1979 0.15 -0.2 2,297,989 - Psyr_1979 0.15 -0.6 2,298,029 + Psyr_1979 0.19 -0.4 2,298,244 + Psyr_1979 0.41 -0.4 2,298,369 + Psyr_1979 0.54 -0.6 2,298,369 + Psyr_1979 0.54 +1.5 2,298,731 + +1.1 2,298,731 + +2.5 2,298,731 + -0.9 2,298,732 - +0.4 2,298,732 - -2.4 2,298,769 + -2.2 2,298,770 - +1.4 2,298,770 - -0.6 2,298,870 + +0.5 2,298,871 - +0.6 2,298,908 - +1.6 2,298,944 + -0.6 2,298,945 - -3.0 2,299,053 + Psyr_1980 0.26 -1.1 2,299,053 + Psyr_1980 0.26 +0.2 2,299,054 - Psyr_1980 0.26 -0.8 2,299,200 + Psyr_1980 0.59 +0.4 2,299,201 - Psyr_1980 0.59 -0.2 2,299,201 - Psyr_1980 0.59 -0.6 2,299,201 - Psyr_1980 0.59 -1.4 2,299,259 + Psyr_1980 0.72 +0.2 2,299,413 + -0.8 2,299,414 - -2.0 2,299,538 + +0.2 2,299,547 + +0.2 2,299,547 + -0.1 2,299,547 - +0.4 2,299,548 - -0.7 2,299,548 - +0.5 2,299,548 - -1.6 2,299,553 + -0.3 2,299,554 - -3.1 2,299,554 - +1.3 2,299,742 + Psyr_1981 0.13 -0.5 2,299,742 + Psyr_1981 0.13 +1.4 2,299,742 + Psyr_1981 0.13 -0.4 2,299,742 + Psyr_1981 0.13 +0.8 2,299,742 + Psyr_1981 0.13 +0.3 2,299,743 - Psyr_1981 0.13 -2.8 2,299,743 - Psyr_1981 0.13 +0.9
Or see this region's nucleotide sequence