Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1971 and Psyr_1972 are separated by 52 nucleotides Psyr_1972 and Psyr_1973 are separated by 271 nucleotides Psyr_1973 and Psyr_1974 are separated by 399 nucleotides
Psyr_1971: Psyr_1971 - Peptidase S45, penicillin amidase, at 2,285,834 to 2,288,173
_1971
Psyr_1972: Psyr_1972 - conserved hypothetical protein, at 2,288,226 to 2,288,537
_1972
Psyr_1973: Psyr_1973 - Aspartate transaminase, at 2,288,809 to 2,290,005
_1973
Psyr_1974: Psyr_1974 - Excinuclease ABC subunit B, at 2,290,405 to 2,292,420
_1974
Position (kb)
2288
2289
2290
2291 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2287.819 kb on + strand, within Psyr_1971 at 2287.898 kb on + strand, within Psyr_1971 at 2287.898 kb on + strand, within Psyr_1971 at 2287.898 kb on + strand, within Psyr_1971 at 2288.136 kb on - strand at 2288.283 kb on + strand, within Psyr_1972 at 2288.284 kb on - strand, within Psyr_1972 at 2288.284 kb on - strand, within Psyr_1972 at 2288.284 kb on - strand, within Psyr_1972 at 2288.323 kb on + strand, within Psyr_1972 at 2288.324 kb on - strand, within Psyr_1972 at 2288.532 kb on - strand at 2288.541 kb on + strand at 2288.542 kb on - strand at 2288.542 kb on - strand at 2288.542 kb on - strand at 2288.542 kb on - strand at 2288.657 kb on - strand at 2288.664 kb on - strand at 2288.664 kb on - strand at 2288.664 kb on - strand at 2288.671 kb on - strand at 2288.671 kb on - strand at 2288.671 kb on - strand at 2288.677 kb on + strand at 2288.677 kb on + strand at 2288.677 kb on + strand at 2288.678 kb on - strand at 2288.678 kb on - strand at 2288.684 kb on + strand at 2288.684 kb on + strand at 2288.691 kb on + strand at 2288.691 kb on + strand at 2288.692 kb on - strand at 2288.692 kb on - strand at 2288.705 kb on + strand at 2288.705 kb on + strand at 2288.705 kb on + strand at 2288.705 kb on + strand at 2288.839 kb on + strand at 2288.839 kb on + strand at 2288.840 kb on - strand at 2288.840 kb on - strand at 2288.840 kb on - strand at 2288.840 kb on - strand at 2288.840 kb on - strand at 2288.878 kb on - strand at 2288.897 kb on + strand at 2288.897 kb on + strand at 2288.898 kb on - strand at 2289.152 kb on + strand, within Psyr_1973 at 2289.152 kb on + strand, within Psyr_1973 at 2289.152 kb on + strand, within Psyr_1973 at 2289.249 kb on - strand, within Psyr_1973 at 2289.551 kb on + strand, within Psyr_1973 at 2290.084 kb on + strand at 2290.084 kb on + strand at 2290.085 kb on - strand at 2290.085 kb on - strand at 2290.085 kb on - strand at 2290.085 kb on - strand at 2290.085 kb on - strand at 2290.087 kb on + strand at 2290.087 kb on + strand at 2290.087 kb on + strand at 2290.087 kb on + strand at 2290.087 kb on + strand at 2290.089 kb on + strand at 2290.090 kb on - strand at 2290.090 kb on - strand at 2290.090 kb on - strand at 2290.149 kb on + strand at 2290.149 kb on + strand at 2290.149 kb on + strand at 2290.150 kb on - strand at 2290.150 kb on - strand at 2290.150 kb on - strand at 2290.156 kb on - strand at 2290.188 kb on + strand at 2290.188 kb on + strand at 2290.212 kb on + strand at 2290.212 kb on + strand at 2290.212 kb on + strand at 2290.212 kb on + strand at 2290.212 kb on + strand at 2290.213 kb on - strand at 2290.213 kb on - strand at 2290.213 kb on - strand at 2290.214 kb on + strand at 2290.214 kb on + strand at 2290.214 kb on + strand at 2290.214 kb on + strand at 2290.214 kb on + strand at 2290.214 kb on + strand at 2290.214 kb on + strand at 2290.214 kb on + strand at 2290.214 kb on + strand at 2290.215 kb on - strand at 2290.215 kb on - strand at 2290.215 kb on - strand at 2290.215 kb on - strand at 2290.215 kb on - strand at 2290.215 kb on - strand at 2290.215 kb on - strand at 2290.216 kb on - strand at 2290.226 kb on + strand at 2290.226 kb on + strand at 2290.227 kb on - strand at 2290.227 kb on - strand at 2290.227 kb on - strand at 2290.404 kb on + strand at 2290.405 kb on - strand at 2290.405 kb on - strand at 2290.405 kb on - strand at 2290.405 kb on - strand at 2290.663 kb on + strand, within Psyr_1974 at 2290.664 kb on - strand, within Psyr_1974 at 2290.664 kb on - strand, within Psyr_1974 at 2290.676 kb on - strand, within Psyr_1974 at 2290.684 kb on + strand, within Psyr_1974 at 2290.684 kb on + strand, within Psyr_1974 at 2290.685 kb on - strand, within Psyr_1974 at 2290.687 kb on + strand, within Psyr_1974 at 2290.687 kb on + strand, within Psyr_1974 at 2290.702 kb on + strand, within Psyr_1974 at 2290.702 kb on + strand, within Psyr_1974 at 2290.702 kb on + strand, within Psyr_1974 at 2290.702 kb on + strand, within Psyr_1974 at 2290.703 kb on - strand, within Psyr_1974 at 2290.703 kb on - strand, within Psyr_1974 at 2290.837 kb on + strand, within Psyr_1974 at 2290.838 kb on - strand, within Psyr_1974 at 2290.861 kb on + strand, within Psyr_1974 at 2290.942 kb on + strand, within Psyr_1974 at 2290.942 kb on + strand, within Psyr_1974 at 2290.943 kb on - strand, within Psyr_1974 at 2290.943 kb on - strand, within Psyr_1974 at 2290.943 kb on - strand, within Psyr_1974
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 2,287,819 + Psyr_1971 0.85 -0.1 2,287,898 + Psyr_1971 0.88 -1.9 2,287,898 + Psyr_1971 0.88 -1.4 2,287,898 + Psyr_1971 0.88 +0.1 2,288,136 - -1.3 2,288,283 + Psyr_1972 0.18 -1.0 2,288,284 - Psyr_1972 0.19 -1.2 2,288,284 - Psyr_1972 0.19 -0.8 2,288,284 - Psyr_1972 0.19 -0.4 2,288,323 + Psyr_1972 0.31 +0.1 2,288,324 - Psyr_1972 0.31 -1.9 2,288,532 - -0.6 2,288,541 + -0.6 2,288,542 - +0.9 2,288,542 - -2.2 2,288,542 - +0.5 2,288,542 - -1.3 2,288,657 - -1.0 2,288,664 - -0.9 2,288,664 - -0.6 2,288,664 - -0.6 2,288,671 - -0.4 2,288,671 - -0.3 2,288,671 - -1.9 2,288,677 + -1.1 2,288,677 + -0.4 2,288,677 + +0.1 2,288,678 - +0.7 2,288,678 - -0.4 2,288,684 + -0.7 2,288,684 + +0.2 2,288,691 + +1.2 2,288,691 + -0.1 2,288,692 - +0.6 2,288,692 - +1.5 2,288,705 + -0.5 2,288,705 + -2.2 2,288,705 + +0.1 2,288,705 + +0.5 2,288,839 + -1.2 2,288,839 + +0.7 2,288,840 - +1.9 2,288,840 - +0.9 2,288,840 - +0.4 2,288,840 - -0.6 2,288,840 - +0.9 2,288,878 - -0.5 2,288,897 + -0.4 2,288,897 + -0.6 2,288,898 - -0.1 2,289,152 + Psyr_1973 0.29 -0.1 2,289,152 + Psyr_1973 0.29 -0.2 2,289,152 + Psyr_1973 0.29 -0.1 2,289,249 - Psyr_1973 0.37 -0.7 2,289,551 + Psyr_1973 0.62 -0.4 2,290,084 + +0.9 2,290,084 + -1.1 2,290,085 - -1.4 2,290,085 - -0.6 2,290,085 - +0.6 2,290,085 - -0.3 2,290,085 - -0.5 2,290,087 + -2.1 2,290,087 + -0.9 2,290,087 + -1.4 2,290,087 + +1.3 2,290,087 + +0.9 2,290,089 + +0.2 2,290,090 - -2.2 2,290,090 - +0.1 2,290,090 - +0.2 2,290,149 + -0.1 2,290,149 + -1.4 2,290,149 + -1.5 2,290,150 - -2.4 2,290,150 - -0.5 2,290,150 - +1.5 2,290,156 - +0.9 2,290,188 + +0.9 2,290,188 + -0.6 2,290,212 + -0.5 2,290,212 + +0.4 2,290,212 + -0.6 2,290,212 + -2.9 2,290,212 + -0.3 2,290,213 - +0.1 2,290,213 - +0.9 2,290,213 - -2.2 2,290,214 + -0.9 2,290,214 + -1.9 2,290,214 + -0.4 2,290,214 + -0.4 2,290,214 + -0.4 2,290,214 + +0.4 2,290,214 + -0.3 2,290,214 + +1.7 2,290,214 + -1.2 2,290,215 - -0.9 2,290,215 - +0.5 2,290,215 - +1.4 2,290,215 - -1.1 2,290,215 - -0.4 2,290,215 - +1.3 2,290,215 - -2.2 2,290,216 - -0.2 2,290,226 + -1.4 2,290,226 + -0.1 2,290,227 - -1.5 2,290,227 - -0.8 2,290,227 - -1.1 2,290,404 + +0.0 2,290,405 - +0.9 2,290,405 - +1.3 2,290,405 - -1.3 2,290,405 - -2.1 2,290,663 + Psyr_1974 0.13 -0.9 2,290,664 - Psyr_1974 0.13 -2.0 2,290,664 - Psyr_1974 0.13 -0.4 2,290,676 - Psyr_1974 0.13 -1.6 2,290,684 + Psyr_1974 0.14 -0.1 2,290,684 + Psyr_1974 0.14 -0.6 2,290,685 - Psyr_1974 0.14 +1.1 2,290,687 + Psyr_1974 0.14 -0.5 2,290,687 + Psyr_1974 0.14 -1.1 2,290,702 + Psyr_1974 0.15 -0.1 2,290,702 + Psyr_1974 0.15 +0.2 2,290,702 + Psyr_1974 0.15 -1.9 2,290,702 + Psyr_1974 0.15 -1.4 2,290,703 - Psyr_1974 0.15 -0.8 2,290,703 - Psyr_1974 0.15 +0.6 2,290,837 + Psyr_1974 0.21 -0.7 2,290,838 - Psyr_1974 0.21 -0.5 2,290,861 + Psyr_1974 0.23 -2.8 2,290,942 + Psyr_1974 0.27 -0.4 2,290,942 + Psyr_1974 0.27 -0.6 2,290,943 - Psyr_1974 0.27 -0.4 2,290,943 - Psyr_1974 0.27 -1.4 2,290,943 - Psyr_1974 0.27 -0.4
Or see this region's nucleotide sequence