Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1963 and Psyr_1964 are separated by 66 nucleotides Psyr_1964 and Psyr_1965 are separated by 47 nucleotides Psyr_1965 and Psyr_1966 overlap by 8 nucleotides
Psyr_1963: Psyr_1963 - Cyclic peptide transporter, at 2,273,616 to 2,275,292
_1963
Psyr_1964: Psyr_1964 - Protein of unknown function DUF323, at 2,275,359 to 2,276,225
_1964
Psyr_1965: Psyr_1965 - Twin-arginine translocation pathway signal, at 2,276,273 to 2,277,553
_1965
Psyr_1966: Psyr_1966 - Peptidase M19, renal dipeptidase, at 2,277,546 to 2,278,940
_1966
Position (kb)
2276
2277
2278 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2275.351 kb on - strand at 2275.351 kb on - strand at 2275.401 kb on + strand at 2275.534 kb on + strand, within Psyr_1964 at 2275.534 kb on + strand, within Psyr_1964 at 2275.534 kb on + strand, within Psyr_1964 at 2275.535 kb on - strand, within Psyr_1964 at 2275.567 kb on + strand, within Psyr_1964 at 2275.588 kb on + strand, within Psyr_1964 at 2275.589 kb on - strand, within Psyr_1964 at 2275.612 kb on + strand, within Psyr_1964 at 2275.634 kb on - strand, within Psyr_1964 at 2275.684 kb on + strand, within Psyr_1964 at 2275.774 kb on + strand, within Psyr_1964 at 2275.810 kb on + strand, within Psyr_1964 at 2275.810 kb on + strand, within Psyr_1964 at 2275.811 kb on - strand, within Psyr_1964 at 2275.811 kb on - strand, within Psyr_1964 at 2275.945 kb on + strand, within Psyr_1964 at 2275.946 kb on - strand, within Psyr_1964 at 2275.946 kb on - strand, within Psyr_1964 at 2275.946 kb on - strand, within Psyr_1964 at 2275.946 kb on - strand, within Psyr_1964 at 2276.178 kb on + strand at 2276.178 kb on + strand at 2276.529 kb on + strand, within Psyr_1965 at 2276.530 kb on - strand, within Psyr_1965 at 2276.645 kb on + strand, within Psyr_1965 at 2276.778 kb on + strand, within Psyr_1965 at 2276.778 kb on + strand, within Psyr_1965 at 2276.779 kb on - strand, within Psyr_1965 at 2276.779 kb on - strand, within Psyr_1965 at 2276.779 kb on - strand, within Psyr_1965 at 2276.779 kb on - strand, within Psyr_1965 at 2276.779 kb on - strand, within Psyr_1965 at 2276.847 kb on + strand, within Psyr_1965 at 2277.163 kb on - strand, within Psyr_1965 at 2277.228 kb on + strand, within Psyr_1965 at 2277.307 kb on + strand, within Psyr_1965 at 2277.393 kb on + strand, within Psyr_1965 at 2277.820 kb on + strand, within Psyr_1966 at 2277.881 kb on - strand, within Psyr_1966 at 2277.895 kb on + strand, within Psyr_1966 at 2278.042 kb on + strand, within Psyr_1966 at 2278.347 kb on - strand, within Psyr_1966 at 2278.358 kb on - strand, within Psyr_1966 at 2278.358 kb on - strand, within Psyr_1966
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 2,275,351 - +0.2 2,275,351 - -0.0 2,275,401 + +1.2 2,275,534 + Psyr_1964 0.20 +0.7 2,275,534 + Psyr_1964 0.20 +2.5 2,275,534 + Psyr_1964 0.20 +0.4 2,275,535 - Psyr_1964 0.20 -0.2 2,275,567 + Psyr_1964 0.24 +0.5 2,275,588 + Psyr_1964 0.26 -1.5 2,275,589 - Psyr_1964 0.27 -0.0 2,275,612 + Psyr_1964 0.29 +0.4 2,275,634 - Psyr_1964 0.32 +1.2 2,275,684 + Psyr_1964 0.37 -1.2 2,275,774 + Psyr_1964 0.48 +0.5 2,275,810 + Psyr_1964 0.52 -0.7 2,275,810 + Psyr_1964 0.52 -1.0 2,275,811 - Psyr_1964 0.52 -0.9 2,275,811 - Psyr_1964 0.52 -0.6 2,275,945 + Psyr_1964 0.68 -0.0 2,275,946 - Psyr_1964 0.68 -0.4 2,275,946 - Psyr_1964 0.68 -1.0 2,275,946 - Psyr_1964 0.68 -1.7 2,275,946 - Psyr_1964 0.68 -1.1 2,276,178 + -0.1 2,276,178 + -0.7 2,276,529 + Psyr_1965 0.20 -0.2 2,276,530 - Psyr_1965 0.20 -1.5 2,276,645 + Psyr_1965 0.29 -0.6 2,276,778 + Psyr_1965 0.39 -1.0 2,276,778 + Psyr_1965 0.39 -1.2 2,276,779 - Psyr_1965 0.40 -0.8 2,276,779 - Psyr_1965 0.40 +0.4 2,276,779 - Psyr_1965 0.40 -0.6 2,276,779 - Psyr_1965 0.40 +1.0 2,276,779 - Psyr_1965 0.40 +0.4 2,276,847 + Psyr_1965 0.45 -2.2 2,277,163 - Psyr_1965 0.69 -1.6 2,277,228 + Psyr_1965 0.75 -0.0 2,277,307 + Psyr_1965 0.81 -0.7 2,277,393 + Psyr_1965 0.87 -0.4 2,277,820 + Psyr_1966 0.20 -0.2 2,277,881 - Psyr_1966 0.24 +0.5 2,277,895 + Psyr_1966 0.25 -0.9 2,278,042 + Psyr_1966 0.36 -0.8 2,278,347 - Psyr_1966 0.57 +2.4 2,278,358 - Psyr_1966 0.58 +2.0 2,278,358 - Psyr_1966 0.58 -0.0
Or see this region's nucleotide sequence