Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1960 and Psyr_1961 are separated by 504 nucleotides Psyr_1961 and Psyr_1962 are separated by 250 nucleotides
Psyr_1960: Psyr_1960 - Non-ribosomal peptide synthase:Amino acid adenylation, at 2,259,252 to 2,267,903
_1960
Psyr_1961: Psyr_1961 - TonB-dependent siderophore receptor, at 2,268,408 to 2,270,819
_1961
Psyr_1962: Psyr_1962 - TonB-dependent siderophore receptor, at 2,271,070 to 2,273,310
_1962
Position (kb)
2268
2269
2270
2271 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2267.439 kb on + strand at 2267.440 kb on - strand at 2267.440 kb on - strand at 2267.977 kb on + strand at 2267.978 kb on - strand at 2268.024 kb on - strand at 2268.203 kb on + strand at 2268.203 kb on + strand at 2268.248 kb on + strand at 2268.248 kb on + strand at 2268.248 kb on + strand at 2268.257 kb on + strand at 2268.257 kb on + strand at 2268.257 kb on + strand at 2268.409 kb on + strand at 2268.436 kb on + strand at 2268.436 kb on + strand at 2268.436 kb on + strand at 2268.436 kb on + strand at 2268.436 kb on + strand at 2268.457 kb on + strand at 2268.490 kb on + strand at 2268.491 kb on - strand at 2268.494 kb on - strand at 2268.494 kb on - strand at 2268.685 kb on + strand, within Psyr_1961 at 2268.686 kb on - strand, within Psyr_1961 at 2268.937 kb on + strand, within Psyr_1961 at 2268.937 kb on + strand, within Psyr_1961 at 2268.937 kb on + strand, within Psyr_1961 at 2268.938 kb on - strand, within Psyr_1961 at 2268.938 kb on - strand, within Psyr_1961 at 2269.078 kb on + strand, within Psyr_1961 at 2269.115 kb on - strand, within Psyr_1961 at 2269.115 kb on - strand, within Psyr_1961 at 2269.189 kb on + strand, within Psyr_1961 at 2269.190 kb on - strand, within Psyr_1961 at 2269.195 kb on + strand, within Psyr_1961 at 2269.195 kb on + strand, within Psyr_1961 at 2269.196 kb on - strand, within Psyr_1961 at 2269.196 kb on - strand, within Psyr_1961 at 2269.270 kb on + strand, within Psyr_1961 at 2269.285 kb on + strand, within Psyr_1961 at 2269.285 kb on + strand, within Psyr_1961 at 2269.285 kb on + strand, within Psyr_1961 at 2269.488 kb on - strand, within Psyr_1961 at 2269.553 kb on + strand, within Psyr_1961 at 2269.568 kb on - strand, within Psyr_1961 at 2269.798 kb on - strand, within Psyr_1961 at 2269.849 kb on + strand, within Psyr_1961 at 2269.975 kb on + strand, within Psyr_1961 at 2270.188 kb on + strand, within Psyr_1961 at 2270.188 kb on + strand, within Psyr_1961 at 2270.188 kb on + strand, within Psyr_1961 at 2270.188 kb on + strand, within Psyr_1961 at 2270.188 kb on + strand, within Psyr_1961 at 2270.188 kb on + strand, within Psyr_1961 at 2270.188 kb on + strand, within Psyr_1961 at 2270.189 kb on - strand, within Psyr_1961 at 2270.189 kb on - strand, within Psyr_1961 at 2270.189 kb on - strand, within Psyr_1961 at 2270.189 kb on - strand, within Psyr_1961 at 2270.189 kb on - strand, within Psyr_1961 at 2270.401 kb on + strand, within Psyr_1961 at 2270.526 kb on + strand, within Psyr_1961 at 2271.053 kb on - strand at 2271.066 kb on + strand at 2271.066 kb on + strand at 2271.066 kb on + strand at 2271.067 kb on - strand at 2271.067 kb on - strand at 2271.067 kb on - strand at 2271.098 kb on + strand at 2271.116 kb on + strand at 2271.152 kb on + strand at 2271.153 kb on - strand at 2271.153 kb on - strand at 2271.153 kb on - strand at 2271.153 kb on - strand at 2271.155 kb on + strand at 2271.156 kb on - strand at 2271.156 kb on - strand at 2271.345 kb on - strand, within Psyr_1962 at 2271.345 kb on - strand, within Psyr_1962 at 2271.422 kb on + strand, within Psyr_1962 at 2271.422 kb on + strand, within Psyr_1962 at 2271.422 kb on + strand, within Psyr_1962 at 2271.423 kb on - strand, within Psyr_1962 at 2271.423 kb on - strand, within Psyr_1962 at 2271.423 kb on - strand, within Psyr_1962 at 2271.423 kb on - strand, within Psyr_1962 at 2271.457 kb on + strand, within Psyr_1962 at 2271.516 kb on - strand, within Psyr_1962 at 2271.549 kb on + strand, within Psyr_1962 at 2271.549 kb on + strand, within Psyr_1962 at 2271.550 kb on - strand, within Psyr_1962 at 2271.550 kb on - strand, within Psyr_1962 at 2271.550 kb on - strand, within Psyr_1962 at 2271.602 kb on + strand, within Psyr_1962 at 2271.603 kb on - strand, within Psyr_1962 at 2271.603 kb on - strand, within Psyr_1962 at 2271.603 kb on - strand, within Psyr_1962 at 2271.603 kb on - strand, within Psyr_1962 at 2271.665 kb on + strand, within Psyr_1962 at 2271.665 kb on + strand, within Psyr_1962 at 2271.713 kb on + strand, within Psyr_1962 at 2271.713 kb on + strand, within Psyr_1962 at 2271.713 kb on + strand, within Psyr_1962 at 2271.713 kb on + strand, within Psyr_1962 at 2271.714 kb on - strand, within Psyr_1962 at 2271.714 kb on - strand, within Psyr_1962 at 2271.743 kb on + strand, within Psyr_1962 at 2271.779 kb on + strand, within Psyr_1962 at 2271.779 kb on + strand, within Psyr_1962 at 2271.779 kb on + strand, within Psyr_1962 at 2271.779 kb on + strand, within Psyr_1962 at 2271.779 kb on + strand, within Psyr_1962 at 2271.779 kb on + strand, within Psyr_1962 at 2271.779 kb on + strand, within Psyr_1962 at 2271.780 kb on - strand, within Psyr_1962 at 2271.783 kb on + strand, within Psyr_1962 at 2271.783 kb on + strand, within Psyr_1962 at 2271.784 kb on - strand, within Psyr_1962 at 2271.784 kb on - strand, within Psyr_1962
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 2,267,439 + +1.0 2,267,440 - +0.4 2,267,440 - -1.0 2,267,977 + +0.1 2,267,978 - +0.3 2,268,024 - -1.5 2,268,203 + +0.6 2,268,203 + -1.2 2,268,248 + -0.4 2,268,248 + -2.2 2,268,248 + -0.6 2,268,257 + -1.1 2,268,257 + -0.5 2,268,257 + -0.0 2,268,409 + -0.3 2,268,436 + +1.0 2,268,436 + -1.0 2,268,436 + +1.5 2,268,436 + -0.6 2,268,436 + +0.1 2,268,457 + +0.4 2,268,490 + -0.9 2,268,491 - -0.8 2,268,494 - -0.4 2,268,494 - -0.8 2,268,685 + Psyr_1961 0.11 +0.1 2,268,686 - Psyr_1961 0.12 -0.6 2,268,937 + Psyr_1961 0.22 -0.4 2,268,937 + Psyr_1961 0.22 -0.9 2,268,937 + Psyr_1961 0.22 -0.3 2,268,938 - Psyr_1961 0.22 -0.2 2,268,938 - Psyr_1961 0.22 -1.4 2,269,078 + Psyr_1961 0.28 +2.5 2,269,115 - Psyr_1961 0.29 -1.0 2,269,115 - Psyr_1961 0.29 +1.0 2,269,189 + Psyr_1961 0.32 -2.4 2,269,190 - Psyr_1961 0.32 -2.0 2,269,195 + Psyr_1961 0.33 +0.4 2,269,195 + Psyr_1961 0.33 +0.5 2,269,196 - Psyr_1961 0.33 -0.9 2,269,196 - Psyr_1961 0.33 +0.4 2,269,270 + Psyr_1961 0.36 -0.9 2,269,285 + Psyr_1961 0.36 -1.1 2,269,285 + Psyr_1961 0.36 -1.4 2,269,285 + Psyr_1961 0.36 -1.6 2,269,488 - Psyr_1961 0.45 -1.0 2,269,553 + Psyr_1961 0.47 -0.3 2,269,568 - Psyr_1961 0.48 -1.7 2,269,798 - Psyr_1961 0.58 -0.0 2,269,849 + Psyr_1961 0.60 -0.2 2,269,975 + Psyr_1961 0.65 -1.7 2,270,188 + Psyr_1961 0.74 +2.0 2,270,188 + Psyr_1961 0.74 -0.5 2,270,188 + Psyr_1961 0.74 -0.6 2,270,188 + Psyr_1961 0.74 -0.4 2,270,188 + Psyr_1961 0.74 -0.4 2,270,188 + Psyr_1961 0.74 -0.0 2,270,188 + Psyr_1961 0.74 +0.4 2,270,189 - Psyr_1961 0.74 -1.5 2,270,189 - Psyr_1961 0.74 -1.0 2,270,189 - Psyr_1961 0.74 -0.9 2,270,189 - Psyr_1961 0.74 +0.6 2,270,189 - Psyr_1961 0.74 +0.5 2,270,401 + Psyr_1961 0.83 -0.1 2,270,526 + Psyr_1961 0.88 -2.0 2,271,053 - +0.1 2,271,066 + +0.4 2,271,066 + -1.7 2,271,066 + +1.0 2,271,067 - -0.6 2,271,067 - -0.6 2,271,067 - -0.2 2,271,098 + +0.2 2,271,116 + +0.6 2,271,152 + -1.4 2,271,153 - -0.4 2,271,153 - -0.0 2,271,153 - -0.9 2,271,153 - -1.4 2,271,155 + -0.9 2,271,156 - -1.4 2,271,156 - -2.0 2,271,345 - Psyr_1962 0.12 +1.0 2,271,345 - Psyr_1962 0.12 -0.7 2,271,422 + Psyr_1962 0.16 -1.4 2,271,422 + Psyr_1962 0.16 -2.0 2,271,422 + Psyr_1962 0.16 -0.0 2,271,423 - Psyr_1962 0.16 -0.5 2,271,423 - Psyr_1962 0.16 -0.6 2,271,423 - Psyr_1962 0.16 -0.0 2,271,423 - Psyr_1962 0.16 +0.4 2,271,457 + Psyr_1962 0.17 +0.1 2,271,516 - Psyr_1962 0.20 +0.4 2,271,549 + Psyr_1962 0.21 +1.3 2,271,549 + Psyr_1962 0.21 +0.5 2,271,550 - Psyr_1962 0.21 -1.6 2,271,550 - Psyr_1962 0.21 -0.4 2,271,550 - Psyr_1962 0.21 +1.7 2,271,602 + Psyr_1962 0.24 -1.9 2,271,603 - Psyr_1962 0.24 -1.5 2,271,603 - Psyr_1962 0.24 -1.6 2,271,603 - Psyr_1962 0.24 -0.7 2,271,603 - Psyr_1962 0.24 -2.9 2,271,665 + Psyr_1962 0.27 -1.6 2,271,665 + Psyr_1962 0.27 -1.0 2,271,713 + Psyr_1962 0.29 +0.4 2,271,713 + Psyr_1962 0.29 -0.6 2,271,713 + Psyr_1962 0.29 -0.9 2,271,713 + Psyr_1962 0.29 +0.2 2,271,714 - Psyr_1962 0.29 -0.7 2,271,714 - Psyr_1962 0.29 -0.0 2,271,743 + Psyr_1962 0.30 -1.0 2,271,779 + Psyr_1962 0.32 -1.9 2,271,779 + Psyr_1962 0.32 -0.0 2,271,779 + Psyr_1962 0.32 -0.0 2,271,779 + Psyr_1962 0.32 -1.4 2,271,779 + Psyr_1962 0.32 -1.6 2,271,779 + Psyr_1962 0.32 -1.4 2,271,779 + Psyr_1962 0.32 -1.5 2,271,780 - Psyr_1962 0.32 -1.3 2,271,783 + Psyr_1962 0.32 -1.4 2,271,783 + Psyr_1962 0.32 -0.0 2,271,784 - Psyr_1962 0.32 -0.3 2,271,784 - Psyr_1962 0.32 -0.0
Or see this region's nucleotide sequence