Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1901 and Psyr_1902 are separated by 42 nucleotides Psyr_1902 and Psyr_1903 are separated by 41 nucleotides Psyr_1903 and Psyr_1904 are separated by 102 nucleotides
Psyr_1901: Psyr_1901 - FAD-dependent pyridine nucleotide-disulfide oxidoreductase:Pyridine nucleotide-disulfide oxidoreductase dimerization region, at 2,176,378 to 2,177,811
_1901
Psyr_1902: Psyr_1902 - Glycerophosphoryl diester phosphodiesterase, at 2,177,854 to 2,178,576
_1902
Psyr_1903: Psyr_1903 - conserved hypothetical protein, at 2,178,618 to 2,179,205
_1903
Psyr_1904: Psyr_1904 - Protein of unknown function DUF214, at 2,179,308 to 2,180,558
_1904
Position (kb)
2178
2179
2180 Strain fitness (log2 ratio)
-2
-1
0
1 at 2178.356 kb on + strand, within Psyr_1902 at 2178.526 kb on - strand at 2178.563 kb on + strand at 2178.567 kb on + strand at 2178.568 kb on - strand at 2178.580 kb on + strand at 2178.596 kb on + strand at 2178.597 kb on - strand at 2178.696 kb on + strand, within Psyr_1903 at 2178.740 kb on + strand, within Psyr_1903 at 2178.742 kb on + strand, within Psyr_1903 at 2178.743 kb on - strand, within Psyr_1903 at 2178.743 kb on - strand, within Psyr_1903 at 2179.171 kb on + strand at 2179.194 kb on + strand at 2179.194 kb on + strand at 2179.194 kb on + strand at 2179.194 kb on + strand at 2179.194 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 2,178,356 + Psyr_1902 0.69 -0.7 2,178,526 - -0.1 2,178,563 + +0.9 2,178,567 + -1.5 2,178,568 - -2.6 2,178,580 + -1.3 2,178,596 + -0.5 2,178,597 - -1.0 2,178,696 + Psyr_1903 0.13 -1.6 2,178,740 + Psyr_1903 0.21 -1.1 2,178,742 + Psyr_1903 0.21 -1.9 2,178,743 - Psyr_1903 0.21 -0.5 2,178,743 - Psyr_1903 0.21 +1.1 2,179,171 + +0.3 2,179,194 + -0.3 2,179,194 + -0.5 2,179,194 + +0.6 2,179,194 + +0.4 2,179,194 + -0.2
Or see this region's nucleotide sequence