Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1775 and Psyr_1776 are separated by 105 nucleotides Psyr_1776 and Psyr_1777 are separated by 354 nucleotides
Psyr_1775: Psyr_1775 - Aldo/keto reductase, at 2,021,862 to 2,022,683
_1775
Psyr_1776: Psyr_1776 - Histidine kinase, HAMP region:Bacterial chemotaxis sensory transducer, at 2,022,789 to 2,024,420
_1776
Psyr_1777: Psyr_1777 - transcriptional regulator, TetR family, at 2,024,775 to 2,025,383
_1777
Position (kb)
2022
2023
2024
2025 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2022.094 kb on + strand, within Psyr_1775 at 2022.094 kb on + strand, within Psyr_1775 at 2022.095 kb on - strand, within Psyr_1775 at 2022.365 kb on - strand, within Psyr_1775 at 2022.365 kb on - strand, within Psyr_1775 at 2022.365 kb on - strand, within Psyr_1775 at 2022.376 kb on + strand, within Psyr_1775 at 2022.377 kb on - strand, within Psyr_1775 at 2022.468 kb on + strand, within Psyr_1775 at 2022.468 kb on + strand, within Psyr_1775 at 2022.469 kb on - strand, within Psyr_1775 at 2022.529 kb on + strand, within Psyr_1775 at 2022.529 kb on + strand, within Psyr_1775 at 2022.529 kb on + strand, within Psyr_1775 at 2022.530 kb on - strand, within Psyr_1775 at 2022.530 kb on - strand, within Psyr_1775 at 2022.530 kb on - strand, within Psyr_1775 at 2022.699 kb on + strand at 2022.699 kb on + strand at 2022.699 kb on + strand at 2022.700 kb on - strand at 2022.700 kb on - strand at 2022.703 kb on + strand at 2022.703 kb on + strand at 2022.704 kb on - strand at 2022.704 kb on - strand at 2022.704 kb on - strand at 2022.711 kb on + strand at 2022.711 kb on + strand at 2023.353 kb on + strand, within Psyr_1776 at 2023.353 kb on + strand, within Psyr_1776 at 2023.353 kb on + strand, within Psyr_1776 at 2023.353 kb on + strand, within Psyr_1776 at 2023.354 kb on - strand, within Psyr_1776 at 2023.354 kb on - strand, within Psyr_1776 at 2023.354 kb on - strand, within Psyr_1776 at 2023.354 kb on - strand, within Psyr_1776 at 2023.354 kb on - strand, within Psyr_1776 at 2023.354 kb on - strand, within Psyr_1776 at 2023.892 kb on - strand, within Psyr_1776 at 2023.892 kb on - strand, within Psyr_1776 at 2023.939 kb on + strand, within Psyr_1776 at 2023.939 kb on + strand, within Psyr_1776 at 2023.940 kb on - strand, within Psyr_1776 at 2023.940 kb on - strand, within Psyr_1776 at 2023.940 kb on - strand, within Psyr_1776 at 2023.940 kb on - strand, within Psyr_1776 at 2024.074 kb on - strand, within Psyr_1776 at 2024.147 kb on - strand, within Psyr_1776 at 2024.175 kb on + strand, within Psyr_1776 at 2024.176 kb on - strand, within Psyr_1776 at 2024.242 kb on + strand, within Psyr_1776 at 2024.242 kb on + strand, within Psyr_1776 at 2024.242 kb on + strand, within Psyr_1776 at 2024.305 kb on + strand at 2024.306 kb on - strand at 2024.415 kb on - strand at 2024.439 kb on + strand at 2024.551 kb on + strand at 2024.573 kb on + strand at 2024.574 kb on - strand at 2024.620 kb on + strand at 2024.620 kb on + strand at 2024.620 kb on + strand at 2024.620 kb on + strand at 2024.620 kb on + strand at 2024.620 kb on + strand at 2024.621 kb on - strand at 2024.836 kb on + strand, within Psyr_1777 at 2024.899 kb on + strand, within Psyr_1777 at 2024.899 kb on + strand, within Psyr_1777 at 2025.126 kb on - strand, within Psyr_1777 at 2025.127 kb on + strand, within Psyr_1777 at 2025.128 kb on - strand at 2025.244 kb on + strand, within Psyr_1777 at 2025.244 kb on + strand, within Psyr_1777 at 2025.244 kb on + strand, within Psyr_1777 at 2025.244 kb on + strand, within Psyr_1777 at 2025.247 kb on + strand, within Psyr_1777 at 2025.247 kb on + strand, within Psyr_1777 at 2025.247 kb on + strand, within Psyr_1777 at 2025.247 kb on + strand, within Psyr_1777 at 2025.248 kb on - strand, within Psyr_1777 at 2025.250 kb on + strand, within Psyr_1777 at 2025.251 kb on - strand, within Psyr_1777 at 2025.251 kb on - strand, within Psyr_1777 at 2025.251 kb on - strand, within Psyr_1777 at 2025.251 kb on - strand, within Psyr_1777
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 2,022,094 + Psyr_1775 0.28 +0.3 2,022,094 + Psyr_1775 0.28 -1.4 2,022,095 - Psyr_1775 0.28 -1.9 2,022,365 - Psyr_1775 0.61 -1.2 2,022,365 - Psyr_1775 0.61 +1.8 2,022,365 - Psyr_1775 0.61 +0.9 2,022,376 + Psyr_1775 0.63 -0.2 2,022,377 - Psyr_1775 0.63 +0.2 2,022,468 + Psyr_1775 0.74 +1.8 2,022,468 + Psyr_1775 0.74 +0.5 2,022,469 - Psyr_1775 0.74 -0.8 2,022,529 + Psyr_1775 0.81 +1.4 2,022,529 + Psyr_1775 0.81 +0.9 2,022,529 + Psyr_1775 0.81 +3.2 2,022,530 - Psyr_1775 0.81 +0.5 2,022,530 - Psyr_1775 0.81 -0.1 2,022,530 - Psyr_1775 0.81 -1.7 2,022,699 + +0.8 2,022,699 + -0.5 2,022,699 + +1.5 2,022,700 - -1.1 2,022,700 - +0.3 2,022,703 + +1.9 2,022,703 + +0.2 2,022,704 - -0.2 2,022,704 - -0.7 2,022,704 - -0.1 2,022,711 + -0.7 2,022,711 + +0.8 2,023,353 + Psyr_1776 0.35 +0.5 2,023,353 + Psyr_1776 0.35 -0.3 2,023,353 + Psyr_1776 0.35 -0.2 2,023,353 + Psyr_1776 0.35 +0.2 2,023,354 - Psyr_1776 0.35 -0.5 2,023,354 - Psyr_1776 0.35 +1.2 2,023,354 - Psyr_1776 0.35 +0.2 2,023,354 - Psyr_1776 0.35 -0.4 2,023,354 - Psyr_1776 0.35 +1.8 2,023,354 - Psyr_1776 0.35 +0.6 2,023,892 - Psyr_1776 0.68 +0.8 2,023,892 - Psyr_1776 0.68 +0.5 2,023,939 + Psyr_1776 0.70 +0.0 2,023,939 + Psyr_1776 0.70 +1.2 2,023,940 - Psyr_1776 0.71 +0.5 2,023,940 - Psyr_1776 0.71 +0.5 2,023,940 - Psyr_1776 0.71 -0.1 2,023,940 - Psyr_1776 0.71 -0.2 2,024,074 - Psyr_1776 0.79 -0.5 2,024,147 - Psyr_1776 0.83 -1.0 2,024,175 + Psyr_1776 0.85 -0.2 2,024,176 - Psyr_1776 0.85 +0.6 2,024,242 + Psyr_1776 0.89 +1.3 2,024,242 + Psyr_1776 0.89 +0.5 2,024,242 + Psyr_1776 0.89 +1.3 2,024,305 + -0.9 2,024,306 - -1.7 2,024,415 - +0.2 2,024,439 + -0.8 2,024,551 + -0.7 2,024,573 + +1.2 2,024,574 - +0.8 2,024,620 + +0.6 2,024,620 + +2.4 2,024,620 + -0.2 2,024,620 + -0.5 2,024,620 + -0.4 2,024,620 + +0.6 2,024,621 - +0.9 2,024,836 + Psyr_1777 0.10 +0.2 2,024,899 + Psyr_1777 0.20 +0.7 2,024,899 + Psyr_1777 0.20 -1.4 2,025,126 - Psyr_1777 0.58 -0.5 2,025,127 + Psyr_1777 0.58 -0.5 2,025,128 - +2.5 2,025,244 + Psyr_1777 0.77 +0.9 2,025,244 + Psyr_1777 0.77 +0.1 2,025,244 + Psyr_1777 0.77 -0.0 2,025,244 + Psyr_1777 0.77 +0.1 2,025,247 + Psyr_1777 0.78 +1.4 2,025,247 + Psyr_1777 0.78 +0.9 2,025,247 + Psyr_1777 0.78 -0.5 2,025,247 + Psyr_1777 0.78 +1.9 2,025,248 - Psyr_1777 0.78 -0.1 2,025,250 + Psyr_1777 0.78 +0.3 2,025,251 - Psyr_1777 0.78 +0.1 2,025,251 - Psyr_1777 0.78 -0.1 2,025,251 - Psyr_1777 0.78 -1.7 2,025,251 - Psyr_1777 0.78 +0.5
Or see this region's nucleotide sequence