Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1765 and Psyr_1766 are separated by 45 nucleotides Psyr_1766 and Psyr_1767 are separated by 195 nucleotides Psyr_1767 and Psyr_1768 are separated by 146 nucleotides
Psyr_1765: Psyr_1765 - GCN5-related N-acetyltransferase, at 2,006,021 to 2,006,569
_1765
Psyr_1766: Psyr_1766 - conserved hypothetical protein, at 2,006,615 to 2,006,830
_1766
Psyr_1767: Psyr_1767 - NADPH-dependent FMN reductase, at 2,007,026 to 2,007,610
_1767
Psyr_1768: Psyr_1768 - conserved hypothetical protein, at 2,007,757 to 2,012,985
_1768
Position (kb)
2007
2008 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2006.072 kb on + strand at 2006.072 kb on + strand at 2006.072 kb on + strand at 2006.072 kb on + strand at 2006.073 kb on - strand at 2006.073 kb on - strand at 2006.073 kb on - strand at 2006.073 kb on - strand at 2006.073 kb on - strand at 2006.098 kb on + strand, within Psyr_1765 at 2006.098 kb on + strand, within Psyr_1765 at 2006.183 kb on + strand, within Psyr_1765 at 2006.275 kb on + strand, within Psyr_1765 at 2006.310 kb on - strand, within Psyr_1765 at 2006.457 kb on + strand, within Psyr_1765 at 2006.458 kb on - strand, within Psyr_1765 at 2006.460 kb on - strand, within Psyr_1765 at 2006.474 kb on + strand, within Psyr_1765 at 2006.474 kb on + strand, within Psyr_1765 at 2006.616 kb on + strand at 2006.617 kb on - strand at 2006.617 kb on - strand at 2006.710 kb on - strand, within Psyr_1766 at 2006.856 kb on + strand at 2006.856 kb on + strand at 2006.857 kb on - strand at 2006.858 kb on + strand at 2006.858 kb on + strand at 2006.858 kb on + strand at 2006.858 kb on + strand at 2006.859 kb on - strand at 2006.859 kb on - strand at 2006.859 kb on - strand at 2006.859 kb on - strand at 2006.859 kb on - strand at 2006.890 kb on + strand at 2006.890 kb on + strand at 2006.891 kb on - strand at 2006.903 kb on + strand at 2006.903 kb on + strand at 2006.904 kb on - strand at 2006.904 kb on - strand at 2006.904 kb on - strand at 2006.904 kb on - strand at 2006.904 kb on - strand at 2006.904 kb on - strand at 2006.904 kb on - strand at 2006.905 kb on + strand at 2006.905 kb on + strand at 2006.906 kb on - strand at 2006.906 kb on - strand at 2006.933 kb on + strand at 2007.001 kb on + strand at 2007.001 kb on + strand at 2007.001 kb on + strand at 2007.001 kb on + strand at 2007.001 kb on + strand at 2007.001 kb on + strand at 2007.002 kb on - strand at 2007.002 kb on - strand at 2007.002 kb on - strand at 2007.002 kb on - strand at 2007.002 kb on - strand at 2007.002 kb on - strand at 2007.002 kb on - strand at 2007.003 kb on + strand at 2007.004 kb on - strand at 2007.004 kb on - strand at 2007.007 kb on + strand at 2007.007 kb on + strand at 2007.008 kb on - strand at 2007.055 kb on + strand at 2007.055 kb on + strand at 2007.056 kb on - strand at 2007.056 kb on - strand at 2007.067 kb on - strand at 2007.103 kb on + strand, within Psyr_1767 at 2007.182 kb on + strand, within Psyr_1767 at 2007.182 kb on + strand, within Psyr_1767 at 2007.182 kb on + strand, within Psyr_1767 at 2007.182 kb on + strand, within Psyr_1767 at 2007.182 kb on + strand, within Psyr_1767 at 2007.183 kb on - strand, within Psyr_1767 at 2007.221 kb on + strand, within Psyr_1767 at 2007.221 kb on + strand, within Psyr_1767 at 2007.222 kb on - strand, within Psyr_1767 at 2007.222 kb on - strand, within Psyr_1767 at 2007.222 kb on - strand, within Psyr_1767 at 2007.222 kb on - strand, within Psyr_1767 at 2007.222 kb on - strand, within Psyr_1767 at 2007.222 kb on - strand, within Psyr_1767 at 2007.222 kb on - strand, within Psyr_1767 at 2007.222 kb on - strand, within Psyr_1767 at 2007.222 kb on - strand, within Psyr_1767 at 2007.222 kb on - strand, within Psyr_1767 at 2007.222 kb on - strand, within Psyr_1767 at 2007.222 kb on - strand, within Psyr_1767 at 2007.222 kb on - strand, within Psyr_1767 at 2007.222 kb on - strand, within Psyr_1767 at 2007.222 kb on - strand, within Psyr_1767 at 2007.222 kb on - strand, within Psyr_1767 at 2007.222 kb on - strand, within Psyr_1767 at 2007.226 kb on + strand, within Psyr_1767 at 2007.226 kb on + strand, within Psyr_1767 at 2007.226 kb on + strand, within Psyr_1767 at 2007.226 kb on + strand, within Psyr_1767 at 2007.227 kb on - strand, within Psyr_1767 at 2007.281 kb on + strand, within Psyr_1767 at 2007.282 kb on - strand, within Psyr_1767 at 2007.282 kb on - strand, within Psyr_1767 at 2007.282 kb on - strand, within Psyr_1767 at 2007.442 kb on - strand, within Psyr_1767 at 2007.480 kb on - strand, within Psyr_1767 at 2007.480 kb on - strand, within Psyr_1767 at 2007.480 kb on - strand, within Psyr_1767 at 2007.640 kb on + strand at 2007.640 kb on + strand at 2007.650 kb on + strand at 2007.650 kb on + strand at 2007.651 kb on - strand at 2007.655 kb on - strand at 2007.729 kb on - strand at 2007.754 kb on + strand at 2007.754 kb on + strand at 2007.754 kb on + strand at 2007.754 kb on + strand at 2007.754 kb on + strand at 2007.754 kb on + strand at 2007.754 kb on + strand at 2007.755 kb on - strand at 2007.755 kb on - strand at 2007.755 kb on - strand at 2007.755 kb on - strand at 2007.755 kb on - strand at 2007.755 kb on - strand at 2007.755 kb on - strand at 2007.755 kb on - strand at 2007.755 kb on - strand at 2007.755 kb on - strand at 2007.775 kb on + strand at 2007.807 kb on + strand at 2007.807 kb on + strand at 2007.807 kb on + strand at 2007.808 kb on - strand at 2007.808 kb on - strand at 2007.847 kb on - strand at 2007.847 kb on - strand at 2008.029 kb on + strand at 2008.029 kb on + strand at 2008.029 kb on + strand at 2008.029 kb on + strand at 2008.029 kb on + strand at 2008.030 kb on - strand at 2008.065 kb on + strand at 2008.066 kb on - strand at 2008.220 kb on + strand at 2008.240 kb on + strand at 2008.240 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 2,006,072 + +1.0 2,006,072 + +1.5 2,006,072 + +0.3 2,006,072 + +0.5 2,006,073 - -1.0 2,006,073 - +1.5 2,006,073 - -0.9 2,006,073 - +0.1 2,006,073 - +0.8 2,006,098 + Psyr_1765 0.14 -0.3 2,006,098 + Psyr_1765 0.14 -0.7 2,006,183 + Psyr_1765 0.30 +0.1 2,006,275 + Psyr_1765 0.46 -0.5 2,006,310 - Psyr_1765 0.53 -1.0 2,006,457 + Psyr_1765 0.79 +0.0 2,006,458 - Psyr_1765 0.80 +1.2 2,006,460 - Psyr_1765 0.80 -0.5 2,006,474 + Psyr_1765 0.83 +1.6 2,006,474 + Psyr_1765 0.83 +1.6 2,006,616 + +0.2 2,006,617 - -0.6 2,006,617 - +0.9 2,006,710 - Psyr_1766 0.44 +0.3 2,006,856 + +1.2 2,006,856 + +0.5 2,006,857 - -0.1 2,006,858 + -0.1 2,006,858 + +0.9 2,006,858 + +0.3 2,006,858 + +0.1 2,006,859 - -0.3 2,006,859 - -0.3 2,006,859 - -1.5 2,006,859 - +0.1 2,006,859 - -1.1 2,006,890 + +0.1 2,006,890 + +0.7 2,006,891 - -0.2 2,006,903 + +0.4 2,006,903 + -0.9 2,006,904 - -0.4 2,006,904 - -0.9 2,006,904 - -1.3 2,006,904 - +0.0 2,006,904 - +1.1 2,006,904 - +1.1 2,006,904 - -1.4 2,006,905 + -0.9 2,006,905 + -0.6 2,006,906 - +0.1 2,006,906 - -1.3 2,006,933 + +1.5 2,007,001 + +0.3 2,007,001 + +0.2 2,007,001 + +0.4 2,007,001 + -0.2 2,007,001 + -1.7 2,007,001 + +0.9 2,007,002 - +0.4 2,007,002 - -0.4 2,007,002 - +1.6 2,007,002 - +0.2 2,007,002 - +1.4 2,007,002 - +0.2 2,007,002 - -0.9 2,007,003 + -0.3 2,007,004 - -0.5 2,007,004 - -2.1 2,007,007 + +0.3 2,007,007 + +0.4 2,007,008 - +0.5 2,007,055 + +1.7 2,007,055 + -0.4 2,007,056 - -1.5 2,007,056 - -1.6 2,007,067 - +0.4 2,007,103 + Psyr_1767 0.13 -0.9 2,007,182 + Psyr_1767 0.27 -0.1 2,007,182 + Psyr_1767 0.27 +0.8 2,007,182 + Psyr_1767 0.27 +0.1 2,007,182 + Psyr_1767 0.27 -1.0 2,007,182 + Psyr_1767 0.27 -1.3 2,007,183 - Psyr_1767 0.27 -0.9 2,007,221 + Psyr_1767 0.33 -0.7 2,007,221 + Psyr_1767 0.33 -0.7 2,007,222 - Psyr_1767 0.34 -0.9 2,007,222 - Psyr_1767 0.34 +0.5 2,007,222 - Psyr_1767 0.34 +0.2 2,007,222 - Psyr_1767 0.34 -0.7 2,007,222 - Psyr_1767 0.34 -0.7 2,007,222 - Psyr_1767 0.34 +0.2 2,007,222 - Psyr_1767 0.34 +0.2 2,007,222 - Psyr_1767 0.34 +0.5 2,007,222 - Psyr_1767 0.34 +1.9 2,007,222 - Psyr_1767 0.34 -0.3 2,007,222 - Psyr_1767 0.34 -1.4 2,007,222 - Psyr_1767 0.34 +1.2 2,007,222 - Psyr_1767 0.34 +0.6 2,007,222 - Psyr_1767 0.34 -0.7 2,007,222 - Psyr_1767 0.34 -0.5 2,007,222 - Psyr_1767 0.34 -2.2 2,007,222 - Psyr_1767 0.34 -1.2 2,007,226 + Psyr_1767 0.34 -0.2 2,007,226 + Psyr_1767 0.34 -0.0 2,007,226 + Psyr_1767 0.34 +0.3 2,007,226 + Psyr_1767 0.34 +0.1 2,007,227 - Psyr_1767 0.34 -1.1 2,007,281 + Psyr_1767 0.44 -0.9 2,007,282 - Psyr_1767 0.44 -0.3 2,007,282 - Psyr_1767 0.44 -0.5 2,007,282 - Psyr_1767 0.44 -0.6 2,007,442 - Psyr_1767 0.71 +1.0 2,007,480 - Psyr_1767 0.78 +0.4 2,007,480 - Psyr_1767 0.78 -0.2 2,007,480 - Psyr_1767 0.78 -0.4 2,007,640 + +0.5 2,007,640 + -1.1 2,007,650 + +2.1 2,007,650 + +2.2 2,007,651 - +0.6 2,007,655 - -0.1 2,007,729 - +0.5 2,007,754 + +0.9 2,007,754 + +0.5 2,007,754 + -0.3 2,007,754 + +0.3 2,007,754 + -2.7 2,007,754 + -2.8 2,007,754 + +0.4 2,007,755 - +0.8 2,007,755 - +0.6 2,007,755 - +0.4 2,007,755 - -0.8 2,007,755 - -1.1 2,007,755 - +1.4 2,007,755 - +0.9 2,007,755 - +0.2 2,007,755 - -0.9 2,007,755 - -0.7 2,007,775 + -0.1 2,007,807 + -0.7 2,007,807 + +0.1 2,007,807 + -0.7 2,007,808 - -1.1 2,007,808 - -0.2 2,007,847 - -0.7 2,007,847 - +0.7 2,008,029 + -1.2 2,008,029 + +0.3 2,008,029 + +0.5 2,008,029 + -0.3 2,008,029 + +1.3 2,008,030 - +0.2 2,008,065 + +0.0 2,008,066 - -0.7 2,008,220 + +0.5 2,008,240 + -0.8 2,008,240 + +0.3
Or see this region's nucleotide sequence