Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1670 and Psyr_RNA28 are separated by 150 nucleotides Psyr_RNA28 and Psyr_RNA29 are separated by 79 nucleotides Psyr_RNA29 and Psyr_RNA30 are separated by 115 nucleotides Psyr_RNA30 and Psyr_1671 are separated by 235 nucleotides Psyr_1671 and Psyr_1672 are separated by 346 nucleotides
Psyr_1670: Psyr_1670 - Short-chain dehydrogenase/reductase SDR, at 1,875,192 to 1,876,010
_1670
Psyr_RNA28: Psyr_RNA28 - tRNA-Val, at 1,876,161 to 1,876,236
_RNA28
Psyr_RNA29: Psyr_RNA29 - tRNA-Asp, at 1,876,316 to 1,876,392
_RNA29
Psyr_RNA30: Psyr_RNA30 - tRNA-Asp, at 1,876,508 to 1,876,584
_RNA30
Psyr_1671: Psyr_1671 - lipoprotein, putative, at 1,876,820 to 1,877,644
_1671
Psyr_1672: Psyr_1672 - L-threonine ammonia-lyase, at 1,877,991 to 1,879,526
_1672
Position (kb)
1876
1877
1878 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1875.887 kb on - strand, within Psyr_1670 at 1875.999 kb on + strand at 1876.000 kb on - strand at 1876.009 kb on - strand at 1876.037 kb on + strand at 1876.038 kb on - strand at 1876.194 kb on + strand, within Psyr_RNA28 at 1876.194 kb on + strand, within Psyr_RNA28 at 1876.457 kb on + strand at 1876.497 kb on + strand at 1876.497 kb on + strand at 1876.585 kb on + strand at 1876.585 kb on + strand at 1876.707 kb on + strand at 1876.708 kb on - strand at 1876.728 kb on + strand at 1876.728 kb on + strand at 1876.728 kb on + strand at 1876.728 kb on + strand at 1876.728 kb on + strand at 1876.728 kb on + strand at 1876.728 kb on + strand at 1876.728 kb on + strand at 1876.729 kb on - strand at 1876.729 kb on - strand at 1876.729 kb on - strand at 1876.729 kb on - strand at 1876.762 kb on - strand at 1876.772 kb on - strand at 1876.772 kb on - strand at 1876.825 kb on + strand at 1876.825 kb on + strand at 1876.826 kb on - strand at 1876.830 kb on + strand at 1876.973 kb on + strand, within Psyr_1671 at 1876.974 kb on - strand, within Psyr_1671 at 1876.974 kb on - strand, within Psyr_1671 at 1877.012 kb on + strand, within Psyr_1671 at 1877.012 kb on + strand, within Psyr_1671 at 1877.012 kb on + strand, within Psyr_1671 at 1877.012 kb on + strand, within Psyr_1671 at 1877.013 kb on - strand, within Psyr_1671 at 1877.013 kb on - strand, within Psyr_1671 at 1877.013 kb on - strand, within Psyr_1671 at 1877.013 kb on - strand, within Psyr_1671 at 1877.014 kb on + strand, within Psyr_1671 at 1877.015 kb on - strand, within Psyr_1671 at 1877.015 kb on - strand, within Psyr_1671 at 1877.015 kb on - strand, within Psyr_1671 at 1877.208 kb on + strand, within Psyr_1671 at 1877.208 kb on + strand, within Psyr_1671 at 1877.209 kb on - strand, within Psyr_1671 at 1877.605 kb on + strand at 1877.606 kb on - strand at 1877.642 kb on + strand at 1877.643 kb on - strand at 1877.643 kb on - strand at 1877.980 kb on + strand at 1878.640 kb on + strand, within Psyr_1672 at 1878.640 kb on + strand, within Psyr_1672 at 1878.640 kb on + strand, within Psyr_1672 at 1878.640 kb on + strand, within Psyr_1672 at 1878.641 kb on - strand, within Psyr_1672 at 1878.641 kb on - strand, within Psyr_1672 at 1878.641 kb on - strand, within Psyr_1672
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 1,875,887 - Psyr_1670 0.85 -0.2 1,875,999 + -0.1 1,876,000 - +0.9 1,876,009 - +0.8 1,876,037 + -0.4 1,876,038 - -0.3 1,876,194 + Psyr_RNA28 0.43 -0.8 1,876,194 + Psyr_RNA28 0.43 +0.9 1,876,457 + +0.8 1,876,497 + +0.1 1,876,497 + +1.2 1,876,585 + +0.9 1,876,585 + +2.5 1,876,707 + -0.2 1,876,708 - -0.9 1,876,728 + +0.5 1,876,728 + +0.3 1,876,728 + +0.2 1,876,728 + +0.3 1,876,728 + +0.2 1,876,728 + -0.1 1,876,728 + +0.1 1,876,728 + -1.9 1,876,729 - -0.4 1,876,729 - -0.7 1,876,729 - +0.6 1,876,729 - -2.4 1,876,762 - +0.7 1,876,772 - +0.6 1,876,772 - -0.1 1,876,825 + -0.3 1,876,825 + -0.8 1,876,826 - +0.8 1,876,830 + +0.6 1,876,973 + Psyr_1671 0.19 +0.1 1,876,974 - Psyr_1671 0.19 +0.2 1,876,974 - Psyr_1671 0.19 +0.6 1,877,012 + Psyr_1671 0.23 -0.1 1,877,012 + Psyr_1671 0.23 -0.3 1,877,012 + Psyr_1671 0.23 -0.5 1,877,012 + Psyr_1671 0.23 -1.4 1,877,013 - Psyr_1671 0.23 +1.9 1,877,013 - Psyr_1671 0.23 -0.3 1,877,013 - Psyr_1671 0.23 -0.5 1,877,013 - Psyr_1671 0.23 -1.7 1,877,014 + Psyr_1671 0.24 +1.1 1,877,015 - Psyr_1671 0.24 -0.6 1,877,015 - Psyr_1671 0.24 -0.3 1,877,015 - Psyr_1671 0.24 -0.7 1,877,208 + Psyr_1671 0.47 -1.1 1,877,208 + Psyr_1671 0.47 -0.2 1,877,209 - Psyr_1671 0.47 -1.2 1,877,605 + -0.1 1,877,606 - +1.0 1,877,642 + +1.1 1,877,643 - +0.6 1,877,643 - -0.3 1,877,980 + +0.7 1,878,640 + Psyr_1672 0.42 -0.8 1,878,640 + Psyr_1672 0.42 -1.7 1,878,640 + Psyr_1672 0.42 -0.7 1,878,640 + Psyr_1672 0.42 -1.4 1,878,641 - Psyr_1672 0.42 -1.5 1,878,641 - Psyr_1672 0.42 +0.4 1,878,641 - Psyr_1672 0.42 +0.2
Or see this region's nucleotide sequence