Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1265 and Psyr_1266 are separated by 176 nucleotides Psyr_1266 and Psyr_1267 overlap by 1 nucleotides Psyr_1267 and Psyr_1268 overlap by 4 nucleotides
Psyr_1265: Psyr_1265 - hypothetical protein, at 1,427,595 to 1,429,571
_1265
Psyr_1266: Psyr_1266 - Twin-arginine translocation pathway signal, at 1,429,748 to 1,431,121
_1266
Psyr_1267: Psyr_1267 - tRNA-adenosine deaminase, at 1,431,121 to 1,431,630
_1267
Psyr_1268: Psyr_1268 - extracellular solute-binding protein, family 3:SLT, at 1,431,627 to 1,433,123
_1268
Position (kb)
1429
1430
1431
1432 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1428.774 kb on + strand, within Psyr_1265 at 1428.776 kb on + strand, within Psyr_1265 at 1428.796 kb on - strand, within Psyr_1265 at 1428.828 kb on + strand, within Psyr_1265 at 1428.828 kb on + strand, within Psyr_1265 at 1428.828 kb on + strand, within Psyr_1265 at 1428.828 kb on + strand, within Psyr_1265 at 1428.829 kb on - strand, within Psyr_1265 at 1428.829 kb on - strand, within Psyr_1265 at 1428.829 kb on - strand, within Psyr_1265 at 1428.829 kb on - strand, within Psyr_1265 at 1428.836 kb on + strand, within Psyr_1265 at 1428.836 kb on + strand, within Psyr_1265 at 1428.836 kb on + strand, within Psyr_1265 at 1428.836 kb on + strand, within Psyr_1265 at 1428.836 kb on + strand, within Psyr_1265 at 1428.836 kb on + strand, within Psyr_1265 at 1428.837 kb on - strand, within Psyr_1265 at 1428.837 kb on - strand, within Psyr_1265 at 1428.837 kb on - strand, within Psyr_1265 at 1428.837 kb on - strand, within Psyr_1265 at 1428.843 kb on + strand, within Psyr_1265 at 1428.843 kb on + strand, within Psyr_1265 at 1428.843 kb on + strand, within Psyr_1265 at 1428.843 kb on + strand, within Psyr_1265 at 1428.843 kb on + strand, within Psyr_1265 at 1428.843 kb on + strand, within Psyr_1265 at 1428.844 kb on - strand, within Psyr_1265 at 1428.844 kb on - strand, within Psyr_1265 at 1428.844 kb on - strand, within Psyr_1265 at 1428.844 kb on - strand, within Psyr_1265 at 1428.844 kb on - strand, within Psyr_1265 at 1428.878 kb on - strand, within Psyr_1265 at 1428.878 kb on - strand, within Psyr_1265 at 1428.936 kb on + strand, within Psyr_1265 at 1428.936 kb on + strand, within Psyr_1265 at 1428.937 kb on - strand, within Psyr_1265 at 1429.081 kb on + strand, within Psyr_1265 at 1429.081 kb on + strand, within Psyr_1265 at 1429.082 kb on - strand, within Psyr_1265 at 1429.150 kb on - strand, within Psyr_1265 at 1429.183 kb on - strand, within Psyr_1265 at 1429.183 kb on - strand, within Psyr_1265 at 1429.289 kb on + strand, within Psyr_1265 at 1429.332 kb on + strand, within Psyr_1265 at 1429.332 kb on + strand, within Psyr_1265 at 1429.332 kb on + strand, within Psyr_1265 at 1429.332 kb on + strand, within Psyr_1265 at 1429.332 kb on + strand, within Psyr_1265 at 1429.332 kb on + strand, within Psyr_1265 at 1429.332 kb on + strand, within Psyr_1265 at 1429.332 kb on + strand, within Psyr_1265 at 1429.332 kb on + strand, within Psyr_1265 at 1429.332 kb on + strand, within Psyr_1265 at 1429.332 kb on + strand, within Psyr_1265 at 1429.332 kb on + strand, within Psyr_1265 at 1429.332 kb on + strand, within Psyr_1265 at 1429.332 kb on + strand, within Psyr_1265 at 1429.332 kb on + strand, within Psyr_1265 at 1429.333 kb on - strand, within Psyr_1265 at 1429.333 kb on - strand, within Psyr_1265 at 1429.333 kb on - strand, within Psyr_1265 at 1429.333 kb on - strand, within Psyr_1265 at 1429.333 kb on - strand, within Psyr_1265 at 1429.333 kb on - strand, within Psyr_1265 at 1429.333 kb on - strand, within Psyr_1265 at 1429.333 kb on - strand, within Psyr_1265 at 1429.333 kb on - strand, within Psyr_1265 at 1429.333 kb on - strand, within Psyr_1265 at 1429.333 kb on - strand, within Psyr_1265 at 1429.333 kb on - strand, within Psyr_1265 at 1429.333 kb on - strand, within Psyr_1265 at 1429.333 kb on - strand, within Psyr_1265 at 1429.333 kb on - strand, within Psyr_1265 at 1429.333 kb on - strand, within Psyr_1265 at 1429.333 kb on - strand, within Psyr_1265 at 1429.333 kb on - strand, within Psyr_1265 at 1429.333 kb on - strand, within Psyr_1265 at 1429.333 kb on - strand, within Psyr_1265 at 1429.513 kb on + strand at 1429.514 kb on - strand at 1429.525 kb on + strand at 1429.526 kb on - strand at 1429.526 kb on - strand at 1429.526 kb on - strand at 1429.526 kb on - strand at 1429.526 kb on - strand at 1429.526 kb on - strand at 1429.536 kb on + strand at 1429.536 kb on + strand at 1429.536 kb on + strand at 1429.536 kb on + strand at 1429.537 kb on - strand at 1429.537 kb on - strand at 1429.537 kb on - strand at 1429.659 kb on + strand at 1429.660 kb on - strand at 1429.660 kb on - strand at 1430.075 kb on + strand, within Psyr_1266 at 1430.075 kb on + strand, within Psyr_1266 at 1430.108 kb on + strand, within Psyr_1266 at 1430.108 kb on + strand, within Psyr_1266 at 1430.147 kb on + strand, within Psyr_1266 at 1430.147 kb on + strand, within Psyr_1266 at 1430.153 kb on + strand, within Psyr_1266 at 1430.153 kb on + strand, within Psyr_1266 at 1430.480 kb on + strand, within Psyr_1266 at 1430.480 kb on + strand, within Psyr_1266 at 1430.480 kb on + strand, within Psyr_1266 at 1430.481 kb on - strand, within Psyr_1266 at 1430.481 kb on - strand, within Psyr_1266 at 1430.481 kb on - strand, within Psyr_1266 at 1430.704 kb on + strand, within Psyr_1266 at 1430.876 kb on + strand, within Psyr_1266 at 1430.876 kb on + strand, within Psyr_1266 at 1430.876 kb on + strand, within Psyr_1266 at 1430.876 kb on + strand, within Psyr_1266 at 1430.876 kb on + strand, within Psyr_1266 at 1430.876 kb on + strand, within Psyr_1266 at 1430.876 kb on + strand, within Psyr_1266 at 1430.876 kb on + strand, within Psyr_1266 at 1430.876 kb on + strand, within Psyr_1266 at 1430.877 kb on - strand, within Psyr_1266 at 1430.877 kb on - strand, within Psyr_1266 at 1430.878 kb on + strand, within Psyr_1266 at 1430.878 kb on + strand, within Psyr_1266 at 1430.879 kb on - strand, within Psyr_1266 at 1430.879 kb on - strand, within Psyr_1266 at 1431.051 kb on + strand at 1431.051 kb on + strand at 1431.051 kb on + strand at 1431.051 kb on + strand at 1431.051 kb on + strand at 1431.051 kb on + strand at 1431.051 kb on + strand at 1431.052 kb on - strand at 1431.052 kb on - strand at 1431.052 kb on - strand at 1431.052 kb on - strand at 1431.052 kb on - strand at 1431.052 kb on - strand at 1431.628 kb on + strand at 1431.905 kb on + strand, within Psyr_1268 at 1431.974 kb on - strand, within Psyr_1268 at 1432.035 kb on + strand, within Psyr_1268 at 1432.035 kb on + strand, within Psyr_1268 at 1432.035 kb on + strand, within Psyr_1268 at 1432.039 kb on + strand, within Psyr_1268 at 1432.039 kb on + strand, within Psyr_1268 at 1432.039 kb on + strand, within Psyr_1268 at 1432.039 kb on + strand, within Psyr_1268 at 1432.040 kb on - strand, within Psyr_1268 at 1432.048 kb on + strand, within Psyr_1268 at 1432.048 kb on + strand, within Psyr_1268
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 1,428,774 + Psyr_1265 0.60 -1.0 1,428,776 + Psyr_1265 0.60 +1.5 1,428,796 - Psyr_1265 0.61 -1.2 1,428,828 + Psyr_1265 0.62 -0.7 1,428,828 + Psyr_1265 0.62 +1.5 1,428,828 + Psyr_1265 0.62 -0.8 1,428,828 + Psyr_1265 0.62 +1.8 1,428,829 - Psyr_1265 0.62 -1.2 1,428,829 - Psyr_1265 0.62 -1.4 1,428,829 - Psyr_1265 0.62 -0.0 1,428,829 - Psyr_1265 0.62 -1.4 1,428,836 + Psyr_1265 0.63 +0.1 1,428,836 + Psyr_1265 0.63 -0.4 1,428,836 + Psyr_1265 0.63 -0.4 1,428,836 + Psyr_1265 0.63 -0.6 1,428,836 + Psyr_1265 0.63 -0.9 1,428,836 + Psyr_1265 0.63 -0.9 1,428,837 - Psyr_1265 0.63 -0.1 1,428,837 - Psyr_1265 0.63 -0.3 1,428,837 - Psyr_1265 0.63 -0.4 1,428,837 - Psyr_1265 0.63 +0.8 1,428,843 + Psyr_1265 0.63 -2.0 1,428,843 + Psyr_1265 0.63 -0.6 1,428,843 + Psyr_1265 0.63 +0.9 1,428,843 + Psyr_1265 0.63 +0.7 1,428,843 + Psyr_1265 0.63 -1.0 1,428,843 + Psyr_1265 0.63 +2.0 1,428,844 - Psyr_1265 0.63 +0.3 1,428,844 - Psyr_1265 0.63 +1.4 1,428,844 - Psyr_1265 0.63 -2.8 1,428,844 - Psyr_1265 0.63 +0.4 1,428,844 - Psyr_1265 0.63 -0.6 1,428,878 - Psyr_1265 0.65 -0.6 1,428,878 - Psyr_1265 0.65 -0.3 1,428,936 + Psyr_1265 0.68 +0.8 1,428,936 + Psyr_1265 0.68 +0.1 1,428,937 - Psyr_1265 0.68 -0.0 1,429,081 + Psyr_1265 0.75 -1.7 1,429,081 + Psyr_1265 0.75 -0.1 1,429,082 - Psyr_1265 0.75 -0.4 1,429,150 - Psyr_1265 0.79 -0.8 1,429,183 - Psyr_1265 0.80 -1.5 1,429,183 - Psyr_1265 0.80 -1.3 1,429,289 + Psyr_1265 0.86 -0.3 1,429,332 + Psyr_1265 0.88 +0.4 1,429,332 + Psyr_1265 0.88 -0.4 1,429,332 + Psyr_1265 0.88 -0.6 1,429,332 + Psyr_1265 0.88 -0.0 1,429,332 + Psyr_1265 0.88 -0.5 1,429,332 + Psyr_1265 0.88 +0.8 1,429,332 + Psyr_1265 0.88 +0.5 1,429,332 + Psyr_1265 0.88 +0.2 1,429,332 + Psyr_1265 0.88 +0.2 1,429,332 + Psyr_1265 0.88 +1.8 1,429,332 + Psyr_1265 0.88 -1.4 1,429,332 + Psyr_1265 0.88 -0.8 1,429,332 + Psyr_1265 0.88 +2.3 1,429,332 + Psyr_1265 0.88 +2.3 1,429,332 + Psyr_1265 0.88 +2.3 1,429,333 - Psyr_1265 0.88 +1.0 1,429,333 - Psyr_1265 0.88 -0.5 1,429,333 - Psyr_1265 0.88 +1.3 1,429,333 - Psyr_1265 0.88 -0.5 1,429,333 - Psyr_1265 0.88 -1.2 1,429,333 - Psyr_1265 0.88 +1.0 1,429,333 - Psyr_1265 0.88 -1.2 1,429,333 - Psyr_1265 0.88 +0.2 1,429,333 - Psyr_1265 0.88 +0.2 1,429,333 - Psyr_1265 0.88 +1.0 1,429,333 - Psyr_1265 0.88 +1.8 1,429,333 - Psyr_1265 0.88 +0.5 1,429,333 - Psyr_1265 0.88 +1.0 1,429,333 - Psyr_1265 0.88 -1.1 1,429,333 - Psyr_1265 0.88 +1.2 1,429,333 - Psyr_1265 0.88 -0.5 1,429,333 - Psyr_1265 0.88 +1.5 1,429,333 - Psyr_1265 0.88 -1.6 1,429,333 - Psyr_1265 0.88 -0.7 1,429,333 - Psyr_1265 0.88 -0.5 1,429,513 + -0.2 1,429,514 - -0.1 1,429,525 + +0.4 1,429,526 - -0.0 1,429,526 - -1.8 1,429,526 - -1.0 1,429,526 - -1.8 1,429,526 - -0.2 1,429,526 - -0.8 1,429,536 + +0.6 1,429,536 + +0.7 1,429,536 + +0.3 1,429,536 + -1.0 1,429,537 - -1.4 1,429,537 - +0.3 1,429,537 - +0.4 1,429,659 + -0.3 1,429,660 - -0.2 1,429,660 - +1.6 1,430,075 + Psyr_1266 0.24 -1.4 1,430,075 + Psyr_1266 0.24 -0.4 1,430,108 + Psyr_1266 0.26 +0.5 1,430,108 + Psyr_1266 0.26 -1.5 1,430,147 + Psyr_1266 0.29 -0.6 1,430,147 + Psyr_1266 0.29 -0.2 1,430,153 + Psyr_1266 0.29 -1.0 1,430,153 + Psyr_1266 0.29 +1.0 1,430,480 + Psyr_1266 0.53 -2.2 1,430,480 + Psyr_1266 0.53 +0.4 1,430,480 + Psyr_1266 0.53 -0.5 1,430,481 - Psyr_1266 0.53 -1.4 1,430,481 - Psyr_1266 0.53 +0.7 1,430,481 - Psyr_1266 0.53 -0.0 1,430,704 + Psyr_1266 0.70 -0.9 1,430,876 + Psyr_1266 0.82 +0.2 1,430,876 + Psyr_1266 0.82 -0.6 1,430,876 + Psyr_1266 0.82 -1.5 1,430,876 + Psyr_1266 0.82 -0.8 1,430,876 + Psyr_1266 0.82 -0.0 1,430,876 + Psyr_1266 0.82 -1.0 1,430,876 + Psyr_1266 0.82 -1.6 1,430,876 + Psyr_1266 0.82 +2.0 1,430,876 + Psyr_1266 0.82 -0.2 1,430,877 - Psyr_1266 0.82 +0.2 1,430,877 - Psyr_1266 0.82 +1.3 1,430,878 + Psyr_1266 0.82 -0.2 1,430,878 + Psyr_1266 0.82 +1.0 1,430,879 - Psyr_1266 0.82 -0.2 1,430,879 - Psyr_1266 0.82 -1.0 1,431,051 + -0.2 1,431,051 + -1.2 1,431,051 + -0.0 1,431,051 + -0.2 1,431,051 + -1.4 1,431,051 + -1.2 1,431,051 + -0.8 1,431,052 - +0.4 1,431,052 - -1.0 1,431,052 - -0.2 1,431,052 - -1.3 1,431,052 - +0.8 1,431,052 - -1.6 1,431,628 + +0.4 1,431,905 + Psyr_1268 0.19 -1.0 1,431,974 - Psyr_1268 0.23 -2.9 1,432,035 + Psyr_1268 0.27 -0.8 1,432,035 + Psyr_1268 0.27 -1.4 1,432,035 + Psyr_1268 0.27 -2.5 1,432,039 + Psyr_1268 0.28 -3.8 1,432,039 + Psyr_1268 0.28 -2.7 1,432,039 + Psyr_1268 0.28 +1.0 1,432,039 + Psyr_1268 0.28 -0.0 1,432,040 - Psyr_1268 0.28 -2.0 1,432,048 + Psyr_1268 0.28 -0.0 1,432,048 + Psyr_1268 0.28 +0.4
Or see this region's nucleotide sequence