Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1259 and Psyr_1260 are separated by 119 nucleotides Psyr_1260 and Psyr_1261 are separated by 94 nucleotides
Psyr_1259: Psyr_1259 - Exodeoxyribonuclease VII large subunit, at 1,415,962 to 1,417,353
_1259
Psyr_1260: Psyr_1260 - conserved hypothetical protein, at 1,417,473 to 1,418,021
_1260
Psyr_1261: Psyr_1261 - inosine-5'-monophosphate dehydrogenase, at 1,418,116 to 1,419,585
_1261
Position (kb)
1417
1418
1419 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1417.040 kb on + strand, within Psyr_1259 at 1417.181 kb on + strand, within Psyr_1259 at 1417.181 kb on + strand, within Psyr_1259 at 1417.181 kb on + strand, within Psyr_1259 at 1417.181 kb on + strand, within Psyr_1259 at 1417.181 kb on + strand, within Psyr_1259 at 1417.181 kb on + strand, within Psyr_1259 at 1417.181 kb on + strand, within Psyr_1259 at 1417.181 kb on + strand, within Psyr_1259 at 1417.182 kb on - strand, within Psyr_1259 at 1417.182 kb on - strand, within Psyr_1259 at 1417.182 kb on - strand, within Psyr_1259 at 1417.182 kb on - strand, within Psyr_1259 at 1417.182 kb on - strand, within Psyr_1259 at 1417.182 kb on - strand, within Psyr_1259 at 1417.182 kb on - strand, within Psyr_1259 at 1417.182 kb on - strand, within Psyr_1259 at 1417.182 kb on - strand, within Psyr_1259 at 1417.182 kb on - strand, within Psyr_1259 at 1417.293 kb on + strand at 1417.294 kb on - strand at 1417.354 kb on - strand at 1417.354 kb on - strand at 1417.354 kb on - strand at 1417.354 kb on - strand at 1417.434 kb on + strand at 1417.458 kb on - strand at 1417.605 kb on + strand, within Psyr_1260 at 1417.605 kb on + strand, within Psyr_1260 at 1417.605 kb on + strand, within Psyr_1260 at 1417.605 kb on + strand, within Psyr_1260 at 1417.605 kb on + strand, within Psyr_1260 at 1417.605 kb on + strand, within Psyr_1260 at 1417.605 kb on + strand, within Psyr_1260 at 1417.606 kb on - strand, within Psyr_1260 at 1417.606 kb on - strand, within Psyr_1260 at 1417.606 kb on - strand, within Psyr_1260 at 1417.606 kb on - strand, within Psyr_1260 at 1417.606 kb on - strand, within Psyr_1260 at 1417.606 kb on - strand, within Psyr_1260 at 1417.607 kb on + strand, within Psyr_1260 at 1417.608 kb on - strand, within Psyr_1260 at 1417.722 kb on + strand, within Psyr_1260 at 1417.722 kb on + strand, within Psyr_1260 at 1417.722 kb on + strand, within Psyr_1260 at 1417.722 kb on + strand, within Psyr_1260 at 1417.722 kb on + strand, within Psyr_1260 at 1417.722 kb on + strand, within Psyr_1260 at 1417.722 kb on + strand, within Psyr_1260 at 1417.722 kb on + strand, within Psyr_1260 at 1417.722 kb on + strand, within Psyr_1260 at 1417.722 kb on + strand, within Psyr_1260 at 1417.722 kb on + strand, within Psyr_1260 at 1417.722 kb on + strand, within Psyr_1260 at 1417.722 kb on + strand, within Psyr_1260 at 1417.722 kb on + strand, within Psyr_1260 at 1417.722 kb on + strand, within Psyr_1260 at 1417.723 kb on - strand, within Psyr_1260 at 1417.723 kb on - strand, within Psyr_1260 at 1417.723 kb on - strand, within Psyr_1260 at 1417.723 kb on - strand, within Psyr_1260 at 1417.723 kb on - strand, within Psyr_1260 at 1417.723 kb on - strand, within Psyr_1260 at 1417.723 kb on - strand, within Psyr_1260 at 1417.723 kb on - strand, within Psyr_1260 at 1417.723 kb on - strand, within Psyr_1260 at 1417.723 kb on - strand, within Psyr_1260 at 1417.723 kb on - strand, within Psyr_1260 at 1417.723 kb on - strand, within Psyr_1260 at 1417.723 kb on - strand, within Psyr_1260 at 1417.723 kb on - strand, within Psyr_1260 at 1417.723 kb on - strand, within Psyr_1260 at 1417.723 kb on - strand, within Psyr_1260 at 1417.928 kb on + strand, within Psyr_1260 at 1418.017 kb on + strand at 1418.017 kb on + strand at 1418.019 kb on + strand at 1418.020 kb on - strand at 1418.035 kb on + strand at 1418.036 kb on - strand at 1418.047 kb on + strand at 1418.047 kb on + strand at 1418.047 kb on + strand at 1418.047 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 1,417,040 + Psyr_1259 0.77 +0.5 1,417,181 + Psyr_1259 0.88 -0.7 1,417,181 + Psyr_1259 0.88 +1.2 1,417,181 + Psyr_1259 0.88 +1.2 1,417,181 + Psyr_1259 0.88 -1.6 1,417,181 + Psyr_1259 0.88 +0.5 1,417,181 + Psyr_1259 0.88 -0.9 1,417,181 + Psyr_1259 0.88 -0.3 1,417,181 + Psyr_1259 0.88 +1.0 1,417,182 - Psyr_1259 0.88 +0.1 1,417,182 - Psyr_1259 0.88 +0.5 1,417,182 - Psyr_1259 0.88 -0.9 1,417,182 - Psyr_1259 0.88 -0.3 1,417,182 - Psyr_1259 0.88 +1.0 1,417,182 - Psyr_1259 0.88 +0.4 1,417,182 - Psyr_1259 0.88 +1.7 1,417,182 - Psyr_1259 0.88 -0.6 1,417,182 - Psyr_1259 0.88 +1.4 1,417,182 - Psyr_1259 0.88 -0.5 1,417,293 + +1.0 1,417,294 - -0.3 1,417,354 - +1.4 1,417,354 - +1.0 1,417,354 - -1.0 1,417,354 - +0.2 1,417,434 + -1.4 1,417,458 - +1.0 1,417,605 + Psyr_1260 0.24 +0.1 1,417,605 + Psyr_1260 0.24 -0.2 1,417,605 + Psyr_1260 0.24 -0.2 1,417,605 + Psyr_1260 0.24 +2.0 1,417,605 + Psyr_1260 0.24 -1.0 1,417,605 + Psyr_1260 0.24 -0.3 1,417,605 + Psyr_1260 0.24 +0.8 1,417,606 - Psyr_1260 0.24 +0.6 1,417,606 - Psyr_1260 0.24 -0.6 1,417,606 - Psyr_1260 0.24 -1.3 1,417,606 - Psyr_1260 0.24 -0.4 1,417,606 - Psyr_1260 0.24 -2.1 1,417,606 - Psyr_1260 0.24 -0.2 1,417,607 + Psyr_1260 0.24 +1.0 1,417,608 - Psyr_1260 0.25 +0.6 1,417,722 + Psyr_1260 0.45 -0.8 1,417,722 + Psyr_1260 0.45 -0.0 1,417,722 + Psyr_1260 0.45 +0.2 1,417,722 + Psyr_1260 0.45 -1.6 1,417,722 + Psyr_1260 0.45 -0.4 1,417,722 + Psyr_1260 0.45 +1.0 1,417,722 + Psyr_1260 0.45 +1.0 1,417,722 + Psyr_1260 0.45 -2.1 1,417,722 + Psyr_1260 0.45 -0.2 1,417,722 + Psyr_1260 0.45 -0.0 1,417,722 + Psyr_1260 0.45 -0.8 1,417,722 + Psyr_1260 0.45 -1.2 1,417,722 + Psyr_1260 0.45 +0.0 1,417,722 + Psyr_1260 0.45 -1.8 1,417,722 + Psyr_1260 0.45 +0.0 1,417,723 - Psyr_1260 0.46 -0.4 1,417,723 - Psyr_1260 0.46 +0.4 1,417,723 - Psyr_1260 0.46 +1.4 1,417,723 - Psyr_1260 0.46 -0.0 1,417,723 - Psyr_1260 0.46 -1.0 1,417,723 - Psyr_1260 0.46 +0.7 1,417,723 - Psyr_1260 0.46 +0.8 1,417,723 - Psyr_1260 0.46 -1.7 1,417,723 - Psyr_1260 0.46 -0.8 1,417,723 - Psyr_1260 0.46 +0.7 1,417,723 - Psyr_1260 0.46 -1.4 1,417,723 - Psyr_1260 0.46 -0.6 1,417,723 - Psyr_1260 0.46 -0.5 1,417,723 - Psyr_1260 0.46 -2.9 1,417,723 - Psyr_1260 0.46 -1.8 1,417,723 - Psyr_1260 0.46 -1.7 1,417,928 + Psyr_1260 0.83 +0.6 1,418,017 + -0.1 1,418,017 + +0.5 1,418,019 + -0.2 1,418,020 - -1.0 1,418,035 + +0.6 1,418,036 - -2.7 1,418,047 + -0.0 1,418,047 + +0.2 1,418,047 + +1.0 1,418,047 + +0.6
Or see this region's nucleotide sequence