Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0967 and Psyr_0968 are separated by 177 nucleotides Psyr_0968 and Psyr_0969 are separated by 4 nucleotides Psyr_0969 and Psyr_0970 are separated by 207 nucleotides
Psyr_0967: Psyr_0967 - ferrochelatase, at 1,100,499 to 1,101,521
_0967
Psyr_0968: Psyr_0968 - Xanthine/uracil permease family, at 1,101,699 to 1,102,976
_0968
Psyr_0969: Psyr_0969 - uracil phosphoribosyltransferase, at 1,102,981 to 1,103,619
_0969
Psyr_0970: Psyr_0970 - Phosphoribosyltransferase, at 1,103,827 to 1,104,384
_0970
Position (kb)
1101
1102
1103 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1101.519 kb on + strand at 1101.519 kb on + strand at 1101.519 kb on + strand at 1101.519 kb on + strand at 1101.519 kb on + strand at 1101.519 kb on + strand at 1101.519 kb on + strand at 1101.519 kb on + strand at 1101.520 kb on - strand at 1101.520 kb on - strand at 1101.520 kb on - strand at 1101.520 kb on - strand at 1101.555 kb on + strand at 1101.840 kb on + strand, within Psyr_0968 at 1101.841 kb on - strand, within Psyr_0968 at 1102.078 kb on + strand, within Psyr_0968 at 1102.078 kb on + strand, within Psyr_0968 at 1102.078 kb on + strand, within Psyr_0968 at 1102.078 kb on + strand, within Psyr_0968 at 1102.078 kb on + strand, within Psyr_0968 at 1102.078 kb on + strand, within Psyr_0968 at 1102.079 kb on - strand, within Psyr_0968 at 1102.079 kb on - strand, within Psyr_0968 at 1102.117 kb on + strand, within Psyr_0968 at 1102.118 kb on - strand, within Psyr_0968 at 1102.118 kb on - strand, within Psyr_0968 at 1102.124 kb on + strand, within Psyr_0968 at 1102.387 kb on + strand, within Psyr_0968 at 1102.388 kb on - strand, within Psyr_0968 at 1102.388 kb on - strand, within Psyr_0968 at 1102.388 kb on - strand, within Psyr_0968 at 1102.388 kb on - strand, within Psyr_0968 at 1102.388 kb on - strand, within Psyr_0968 at 1102.682 kb on + strand, within Psyr_0968 at 1102.682 kb on + strand, within Psyr_0968 at 1102.682 kb on + strand, within Psyr_0968 at 1102.682 kb on + strand, within Psyr_0968 at 1102.682 kb on + strand, within Psyr_0968 at 1102.683 kb on - strand, within Psyr_0968 at 1102.683 kb on - strand, within Psyr_0968 at 1102.683 kb on - strand, within Psyr_0968 at 1102.683 kb on - strand, within Psyr_0968 at 1102.684 kb on + strand, within Psyr_0968 at 1102.684 kb on + strand, within Psyr_0968 at 1102.684 kb on + strand, within Psyr_0968 at 1102.684 kb on + strand, within Psyr_0968 at 1102.685 kb on - strand, within Psyr_0968 at 1102.685 kb on - strand, within Psyr_0968 at 1103.045 kb on + strand, within Psyr_0969 at 1103.046 kb on - strand, within Psyr_0969 at 1103.046 kb on - strand, within Psyr_0969 at 1103.046 kb on - strand, within Psyr_0969 at 1103.084 kb on + strand, within Psyr_0969 at 1103.084 kb on + strand, within Psyr_0969 at 1103.085 kb on - strand, within Psyr_0969 at 1103.085 kb on - strand, within Psyr_0969 at 1103.282 kb on + strand, within Psyr_0969 at 1103.282 kb on + strand, within Psyr_0969 at 1103.465 kb on + strand, within Psyr_0969 at 1103.466 kb on - strand, within Psyr_0969 at 1103.466 kb on - strand, within Psyr_0969 at 1103.466 kb on - strand, within Psyr_0969 at 1103.466 kb on - strand, within Psyr_0969 at 1103.498 kb on + strand, within Psyr_0969 at 1103.498 kb on + strand, within Psyr_0969 at 1103.498 kb on + strand, within Psyr_0969 at 1103.498 kb on + strand, within Psyr_0969 at 1103.498 kb on + strand, within Psyr_0969 at 1103.498 kb on + strand, within Psyr_0969 at 1103.498 kb on + strand, within Psyr_0969 at 1103.498 kb on + strand, within Psyr_0969 at 1103.498 kb on + strand, within Psyr_0969 at 1103.498 kb on + strand, within Psyr_0969 at 1103.498 kb on + strand, within Psyr_0969 at 1103.499 kb on - strand, within Psyr_0969 at 1103.499 kb on - strand, within Psyr_0969 at 1103.499 kb on - strand, within Psyr_0969 at 1103.499 kb on - strand, within Psyr_0969 at 1103.569 kb on - strand at 1103.587 kb on + strand at 1103.588 kb on - strand at 1103.652 kb on + strand at 1103.652 kb on + strand at 1103.652 kb on + strand at 1103.652 kb on + strand at 1103.653 kb on - strand at 1103.653 kb on - strand at 1103.653 kb on - strand at 1103.653 kb on - strand at 1103.662 kb on + strand at 1103.663 kb on - strand at 1103.681 kb on + strand at 1103.682 kb on - strand at 1103.746 kb on + strand at 1103.783 kb on + strand at 1103.784 kb on - strand at 1103.812 kb on + strand at 1103.881 kb on + strand at 1103.882 kb on - strand at 1103.882 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 1,101,519 + +0.4 1,101,519 + -1.3 1,101,519 + -0.2 1,101,519 + -1.4 1,101,519 + +0.3 1,101,519 + -0.0 1,101,519 + +0.9 1,101,519 + -0.5 1,101,520 - -0.1 1,101,520 - +0.2 1,101,520 - +0.0 1,101,520 - +1.5 1,101,555 + -0.7 1,101,840 + Psyr_0968 0.11 -1.4 1,101,841 - Psyr_0968 0.11 +1.9 1,102,078 + Psyr_0968 0.30 -0.2 1,102,078 + Psyr_0968 0.30 -0.3 1,102,078 + Psyr_0968 0.30 -1.2 1,102,078 + Psyr_0968 0.30 -0.4 1,102,078 + Psyr_0968 0.30 -1.9 1,102,078 + Psyr_0968 0.30 -1.9 1,102,079 - Psyr_0968 0.30 +1.5 1,102,079 - Psyr_0968 0.30 -0.6 1,102,117 + Psyr_0968 0.33 -0.5 1,102,118 - Psyr_0968 0.33 +0.5 1,102,118 - Psyr_0968 0.33 -2.3 1,102,124 + Psyr_0968 0.33 -0.6 1,102,387 + Psyr_0968 0.54 -0.1 1,102,388 - Psyr_0968 0.54 -1.1 1,102,388 - Psyr_0968 0.54 -0.4 1,102,388 - Psyr_0968 0.54 -1.9 1,102,388 - Psyr_0968 0.54 -1.3 1,102,388 - Psyr_0968 0.54 +0.5 1,102,682 + Psyr_0968 0.77 -0.4 1,102,682 + Psyr_0968 0.77 +0.7 1,102,682 + Psyr_0968 0.77 -0.5 1,102,682 + Psyr_0968 0.77 +0.3 1,102,682 + Psyr_0968 0.77 -1.4 1,102,683 - Psyr_0968 0.77 +0.9 1,102,683 - Psyr_0968 0.77 -1.4 1,102,683 - Psyr_0968 0.77 +0.9 1,102,683 - Psyr_0968 0.77 -0.7 1,102,684 + Psyr_0968 0.77 -1.4 1,102,684 + Psyr_0968 0.77 +0.5 1,102,684 + Psyr_0968 0.77 -0.1 1,102,684 + Psyr_0968 0.77 -2.7 1,102,685 - Psyr_0968 0.77 -1.1 1,102,685 - Psyr_0968 0.77 +0.3 1,103,045 + Psyr_0969 0.10 +0.2 1,103,046 - Psyr_0969 0.10 -0.0 1,103,046 - Psyr_0969 0.10 -0.1 1,103,046 - Psyr_0969 0.10 +0.6 1,103,084 + Psyr_0969 0.16 -1.3 1,103,084 + Psyr_0969 0.16 +0.9 1,103,085 - Psyr_0969 0.16 -1.1 1,103,085 - Psyr_0969 0.16 -0.4 1,103,282 + Psyr_0969 0.47 -0.2 1,103,282 + Psyr_0969 0.47 +0.4 1,103,465 + Psyr_0969 0.76 +0.1 1,103,466 - Psyr_0969 0.76 -0.9 1,103,466 - Psyr_0969 0.76 -0.9 1,103,466 - Psyr_0969 0.76 +0.7 1,103,466 - Psyr_0969 0.76 +1.4 1,103,498 + Psyr_0969 0.81 +0.5 1,103,498 + Psyr_0969 0.81 +0.2 1,103,498 + Psyr_0969 0.81 -0.4 1,103,498 + Psyr_0969 0.81 -0.3 1,103,498 + Psyr_0969 0.81 -2.5 1,103,498 + Psyr_0969 0.81 +0.5 1,103,498 + Psyr_0969 0.81 +0.9 1,103,498 + Psyr_0969 0.81 +0.3 1,103,498 + Psyr_0969 0.81 +0.1 1,103,498 + Psyr_0969 0.81 -0.3 1,103,498 + Psyr_0969 0.81 -0.6 1,103,499 - Psyr_0969 0.81 +0.0 1,103,499 - Psyr_0969 0.81 -1.4 1,103,499 - Psyr_0969 0.81 +0.3 1,103,499 - Psyr_0969 0.81 +0.6 1,103,569 - +0.9 1,103,587 + -0.9 1,103,588 - +0.4 1,103,652 + -2.3 1,103,652 + +0.9 1,103,652 + +1.2 1,103,652 + +1.1 1,103,653 - +0.9 1,103,653 - -0.9 1,103,653 - -0.5 1,103,653 - +1.3 1,103,662 + -0.0 1,103,663 - -0.0 1,103,681 + +1.2 1,103,682 - -1.5 1,103,746 + -0.8 1,103,783 + -0.3 1,103,784 - -0.5 1,103,812 + -0.1 1,103,881 + -0.1 1,103,882 - -0.7 1,103,882 - +0.3
Or see this region's nucleotide sequence