Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0895 and Psyr_0896 are separated by 115 nucleotides Psyr_0896 and Psyr_0897 overlap by 26 nucleotides Psyr_0897 and Psyr_0898 are separated by 84 nucleotides
Psyr_0895: Psyr_0895 - SpoVT/AbrB-like protein, at 1,014,927 to 1,015,157
_0895
Psyr_0896: Psyr_0896 - Aldose 1-epimerase, at 1,015,273 to 1,016,154
_0896
Psyr_0897: Psyr_0897 - Protein of unknown function DUF6, at 1,016,129 to 1,017,100
_0897
Psyr_0898: Psyr_0898 - TRAP dicarboxylate transporter, DctM subunit, at 1,017,185 to 1,018,465
_0898
Position (kb)
1016
1017
1018 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1015.192 kb on + strand at 1015.192 kb on + strand at 1015.193 kb on - strand at 1015.193 kb on - strand at 1015.198 kb on + strand at 1015.198 kb on + strand at 1015.198 kb on + strand at 1015.198 kb on + strand at 1015.244 kb on - strand at 1015.270 kb on + strand at 1015.270 kb on + strand at 1015.270 kb on + strand at 1015.270 kb on + strand at 1015.270 kb on + strand at 1015.270 kb on + strand at 1015.270 kb on + strand at 1015.270 kb on + strand at 1015.270 kb on + strand at 1015.270 kb on + strand at 1015.270 kb on + strand at 1015.270 kb on + strand at 1015.270 kb on + strand at 1015.270 kb on + strand at 1015.270 kb on + strand at 1015.270 kb on + strand at 1015.270 kb on + strand at 1015.271 kb on - strand at 1015.271 kb on - strand at 1015.271 kb on - strand at 1015.271 kb on - strand at 1015.271 kb on - strand at 1015.271 kb on - strand at 1015.271 kb on - strand at 1015.271 kb on - strand at 1015.271 kb on - strand at 1015.271 kb on - strand at 1015.298 kb on + strand at 1015.445 kb on + strand, within Psyr_0896 at 1015.446 kb on - strand, within Psyr_0896 at 1015.446 kb on - strand, within Psyr_0896 at 1015.446 kb on - strand, within Psyr_0896 at 1015.446 kb on - strand, within Psyr_0896 at 1015.683 kb on - strand, within Psyr_0896 at 1015.772 kb on + strand, within Psyr_0896 at 1015.773 kb on - strand, within Psyr_0896 at 1015.773 kb on - strand, within Psyr_0896 at 1016.140 kb on + strand at 1016.140 kb on + strand at 1016.140 kb on + strand at 1016.140 kb on + strand at 1016.140 kb on + strand at 1016.140 kb on + strand at 1016.141 kb on - strand at 1016.141 kb on - strand at 1016.141 kb on - strand at 1016.141 kb on - strand at 1016.141 kb on - strand at 1016.141 kb on - strand at 1016.141 kb on - strand at 1016.525 kb on + strand, within Psyr_0897 at 1016.526 kb on - strand, within Psyr_0897 at 1016.604 kb on + strand, within Psyr_0897 at 1016.812 kb on + strand, within Psyr_0897 at 1016.812 kb on + strand, within Psyr_0897 at 1016.812 kb on + strand, within Psyr_0897 at 1016.813 kb on - strand, within Psyr_0897 at 1016.813 kb on - strand, within Psyr_0897 at 1016.813 kb on - strand, within Psyr_0897 at 1016.813 kb on - strand, within Psyr_0897 at 1016.829 kb on + strand, within Psyr_0897 at 1016.830 kb on - strand, within Psyr_0897 at 1016.830 kb on - strand, within Psyr_0897 at 1016.830 kb on - strand, within Psyr_0897 at 1016.830 kb on - strand, within Psyr_0897 at 1016.830 kb on - strand, within Psyr_0897 at 1017.100 kb on + strand at 1017.100 kb on + strand at 1017.100 kb on + strand at 1017.100 kb on + strand at 1017.100 kb on + strand at 1017.100 kb on + strand at 1017.101 kb on - strand at 1017.101 kb on - strand at 1017.228 kb on + strand at 1017.228 kb on + strand at 1017.228 kb on + strand at 1017.228 kb on + strand at 1017.228 kb on + strand at 1017.228 kb on + strand at 1017.228 kb on + strand at 1017.228 kb on + strand at 1017.228 kb on + strand at 1017.229 kb on - strand at 1017.229 kb on - strand at 1017.229 kb on - strand at 1017.229 kb on - strand at 1017.229 kb on - strand at 1017.229 kb on - strand at 1017.229 kb on - strand at 1017.264 kb on + strand at 1017.264 kb on + strand at 1017.264 kb on + strand at 1017.264 kb on + strand at 1017.265 kb on - strand at 1017.265 kb on - strand at 1017.265 kb on - strand at 1017.265 kb on - strand at 1017.265 kb on - strand at 1017.265 kb on - strand at 1017.265 kb on - strand at 1017.265 kb on - strand at 1017.265 kb on - strand at 1017.265 kb on - strand at 1017.265 kb on - strand at 1017.284 kb on + strand at 1017.285 kb on - strand at 1017.285 kb on - strand at 1017.285 kb on - strand at 1017.285 kb on - strand at 1017.525 kb on + strand, within Psyr_0898 at 1017.594 kb on + strand, within Psyr_0898 at 1017.595 kb on - strand, within Psyr_0898 at 1017.595 kb on - strand, within Psyr_0898 at 1017.687 kb on + strand, within Psyr_0898 at 1017.688 kb on - strand, within Psyr_0898 at 1017.787 kb on + strand, within Psyr_0898 at 1017.787 kb on + strand, within Psyr_0898 at 1017.788 kb on - strand, within Psyr_0898 at 1017.788 kb on - strand, within Psyr_0898 at 1017.993 kb on + strand, within Psyr_0898 at 1017.994 kb on - strand, within Psyr_0898 at 1017.994 kb on - strand, within Psyr_0898 at 1017.994 kb on - strand, within Psyr_0898
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 1,015,192 + +0.4 1,015,192 + -0.5 1,015,193 - +0.5 1,015,193 - +0.2 1,015,198 + +1.1 1,015,198 + -0.5 1,015,198 + -0.4 1,015,198 + -1.2 1,015,244 - -2.4 1,015,270 + +0.3 1,015,270 + +1.2 1,015,270 + +2.5 1,015,270 + +0.8 1,015,270 + -0.3 1,015,270 + -1.1 1,015,270 + +0.1 1,015,270 + +0.9 1,015,270 + -0.2 1,015,270 + +0.6 1,015,270 + -0.0 1,015,270 + +1.5 1,015,270 + +0.0 1,015,270 + +0.0 1,015,270 + +2.0 1,015,270 + +1.9 1,015,270 + -0.3 1,015,271 - +2.5 1,015,271 - -1.5 1,015,271 - -1.4 1,015,271 - +0.4 1,015,271 - -0.1 1,015,271 - -1.2 1,015,271 - +1.0 1,015,271 - +0.9 1,015,271 - +0.9 1,015,271 - +0.6 1,015,298 + +1.2 1,015,445 + Psyr_0896 0.20 -0.6 1,015,446 - Psyr_0896 0.20 +0.4 1,015,446 - Psyr_0896 0.20 -0.7 1,015,446 - Psyr_0896 0.20 -0.1 1,015,446 - Psyr_0896 0.20 -0.1 1,015,683 - Psyr_0896 0.46 -0.1 1,015,772 + Psyr_0896 0.57 +0.7 1,015,773 - Psyr_0896 0.57 +1.3 1,015,773 - Psyr_0896 0.57 +0.6 1,016,140 + -0.1 1,016,140 + -0.1 1,016,140 + +0.4 1,016,140 + -0.3 1,016,140 + +2.2 1,016,140 + +0.5 1,016,141 - -0.3 1,016,141 - +2.1 1,016,141 - +1.5 1,016,141 - +1.1 1,016,141 - +0.1 1,016,141 - +1.0 1,016,141 - +0.6 1,016,525 + Psyr_0897 0.41 +0.3 1,016,526 - Psyr_0897 0.41 +1.0 1,016,604 + Psyr_0897 0.49 +0.7 1,016,812 + Psyr_0897 0.70 +1.1 1,016,812 + Psyr_0897 0.70 +1.9 1,016,812 + Psyr_0897 0.70 +0.7 1,016,813 - Psyr_0897 0.70 +1.6 1,016,813 - Psyr_0897 0.70 +0.9 1,016,813 - Psyr_0897 0.70 -0.3 1,016,813 - Psyr_0897 0.70 -1.1 1,016,829 + Psyr_0897 0.72 -0.1 1,016,830 - Psyr_0897 0.72 +0.9 1,016,830 - Psyr_0897 0.72 +1.2 1,016,830 - Psyr_0897 0.72 +0.8 1,016,830 - Psyr_0897 0.72 +1.3 1,016,830 - Psyr_0897 0.72 +0.6 1,017,100 + -1.4 1,017,100 + +1.0 1,017,100 + -0.8 1,017,100 + -0.1 1,017,100 + +0.4 1,017,100 + +0.9 1,017,101 - -1.3 1,017,101 - -1.1 1,017,228 + -0.6 1,017,228 + +0.3 1,017,228 + +1.1 1,017,228 + +0.4 1,017,228 + -0.5 1,017,228 + -1.4 1,017,228 + +0.2 1,017,228 + +0.9 1,017,228 + -0.2 1,017,229 - -1.1 1,017,229 - +0.1 1,017,229 - +1.5 1,017,229 - +0.3 1,017,229 - -0.7 1,017,229 - -0.1 1,017,229 - +2.1 1,017,264 + -0.1 1,017,264 + +0.9 1,017,264 + -2.4 1,017,264 + -1.8 1,017,265 - +1.6 1,017,265 - +0.1 1,017,265 - -1.9 1,017,265 - +0.6 1,017,265 - -1.0 1,017,265 - +0.4 1,017,265 - +0.7 1,017,265 - +0.9 1,017,265 - -0.8 1,017,265 - -0.4 1,017,265 - +0.4 1,017,284 + +1.6 1,017,285 - -0.8 1,017,285 - +0.3 1,017,285 - +1.5 1,017,285 - +0.3 1,017,525 + Psyr_0898 0.27 -0.7 1,017,594 + Psyr_0898 0.32 -0.1 1,017,595 - Psyr_0898 0.32 -1.0 1,017,595 - Psyr_0898 0.32 +0.1 1,017,687 + Psyr_0898 0.39 +0.6 1,017,688 - Psyr_0898 0.39 -0.4 1,017,787 + Psyr_0898 0.47 +1.6 1,017,787 + Psyr_0898 0.47 +0.5 1,017,788 - Psyr_0898 0.47 -0.6 1,017,788 - Psyr_0898 0.47 -0.7 1,017,993 + Psyr_0898 0.63 -0.1 1,017,994 - Psyr_0898 0.63 +0.7 1,017,994 - Psyr_0898 0.63 -0.7 1,017,994 - Psyr_0898 0.63 +0.1
Or see this region's nucleotide sequence