Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0577 and Psyr_0578 are separated by 171 nucleotides Psyr_0578 and Psyr_RNA2 are separated by 106 nucleotides Psyr_RNA2 and Psyr_RNA3 are separated by 160 nucleotides Psyr_RNA3 and Psyr_0579 are separated by 225 nucleotides
Psyr_0577: Psyr_0577 - Adenylosuccinate synthetase, at 655,874 to 657,166
_0577
Psyr_0578: Psyr_0578 - Histidine kinase, HAMP region:Bacterial chemotaxis sensory transducer, at 657,338 to 659,272
_0578
Psyr_RNA2: Psyr_RNA2 - tRNA-Leu, at 659,379 to 659,465
_RNA2
Psyr_RNA3: Psyr_RNA3 - tRNA-Leu, at 659,626 to 659,712
_RNA3
Psyr_0579: Psyr_0579 - RNAse R, at 659,938 to 662,571
_0579
Position (kb) 657
658
659
660 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 657.167 kb on + strand at 657.167 kb on + strand at 657.167 kb on + strand at 657.167 kb on + strand at 657.168 kb on - strand at 657.168 kb on - strand at 657.278 kb on + strand at 657.278 kb on + strand at 657.278 kb on + strand at 657.279 kb on - strand at 657.279 kb on - strand at 657.279 kb on - strand at 657.351 kb on + strand at 657.351 kb on + strand at 657.351 kb on + strand at 657.351 kb on + strand at 657.352 kb on - strand at 657.352 kb on - strand at 657.352 kb on - strand at 657.352 kb on - strand at 657.352 kb on - strand at 657.352 kb on - strand at 657.352 kb on - strand at 657.352 kb on - strand at 657.352 kb on - strand at 657.352 kb on - strand at 657.352 kb on - strand at 657.493 kb on - strand at 657.602 kb on - strand, within Psyr_0578 at 657.774 kb on + strand, within Psyr_0578 at 657.774 kb on + strand, within Psyr_0578 at 657.774 kb on + strand, within Psyr_0578 at 657.775 kb on - strand, within Psyr_0578 at 657.775 kb on - strand, within Psyr_0578 at 657.775 kb on - strand, within Psyr_0578 at 657.775 kb on - strand, within Psyr_0578 at 657.775 kb on - strand, within Psyr_0578 at 657.775 kb on - strand, within Psyr_0578 at 657.775 kb on - strand, within Psyr_0578 at 657.775 kb on - strand, within Psyr_0578 at 657.775 kb on - strand, within Psyr_0578 at 657.775 kb on - strand, within Psyr_0578 at 657.775 kb on - strand, within Psyr_0578 at 657.788 kb on + strand, within Psyr_0578 at 657.814 kb on + strand, within Psyr_0578 at 657.815 kb on - strand, within Psyr_0578 at 657.821 kb on + strand, within Psyr_0578 at 657.822 kb on - strand, within Psyr_0578 at 657.822 kb on - strand, within Psyr_0578 at 657.822 kb on - strand, within Psyr_0578 at 657.822 kb on - strand, within Psyr_0578 at 657.822 kb on - strand, within Psyr_0578 at 657.926 kb on + strand, within Psyr_0578 at 657.926 kb on + strand, within Psyr_0578 at 657.926 kb on + strand, within Psyr_0578 at 657.927 kb on - strand, within Psyr_0578 at 657.927 kb on - strand, within Psyr_0578 at 657.927 kb on - strand, within Psyr_0578 at 657.927 kb on - strand, within Psyr_0578 at 657.927 kb on - strand, within Psyr_0578 at 657.927 kb on - strand, within Psyr_0578 at 657.927 kb on - strand, within Psyr_0578 at 657.927 kb on - strand, within Psyr_0578 at 657.929 kb on + strand, within Psyr_0578 at 657.930 kb on - strand, within Psyr_0578 at 657.930 kb on - strand, within Psyr_0578 at 657.930 kb on - strand, within Psyr_0578 at 657.930 kb on - strand, within Psyr_0578 at 657.987 kb on + strand, within Psyr_0578 at 657.988 kb on - strand, within Psyr_0578 at 658.245 kb on + strand, within Psyr_0578 at 658.245 kb on + strand, within Psyr_0578 at 658.246 kb on - strand, within Psyr_0578 at 658.255 kb on - strand, within Psyr_0578 at 658.387 kb on + strand, within Psyr_0578 at 658.387 kb on + strand, within Psyr_0578 at 658.388 kb on - strand, within Psyr_0578 at 658.388 kb on - strand, within Psyr_0578 at 658.388 kb on - strand, within Psyr_0578 at 658.388 kb on - strand, within Psyr_0578 at 658.388 kb on - strand, within Psyr_0578 at 658.388 kb on - strand, within Psyr_0578 at 658.429 kb on + strand, within Psyr_0578 at 658.430 kb on - strand, within Psyr_0578 at 658.660 kb on + strand, within Psyr_0578 at 658.661 kb on - strand, within Psyr_0578 at 658.661 kb on - strand, within Psyr_0578 at 658.786 kb on + strand, within Psyr_0578 at 658.825 kb on + strand, within Psyr_0578 at 658.826 kb on - strand, within Psyr_0578 at 658.924 kb on + strand, within Psyr_0578 at 658.924 kb on + strand, within Psyr_0578 at 659.019 kb on + strand, within Psyr_0578 at 659.020 kb on - strand, within Psyr_0578 at 659.482 kb on - strand at 659.502 kb on + strand at 659.502 kb on + strand at 659.504 kb on + strand at 659.505 kb on - strand at 659.505 kb on - strand at 659.525 kb on + strand at 659.543 kb on + strand at 659.543 kb on + strand at 659.543 kb on + strand at 659.544 kb on - strand at 659.547 kb on - strand at 659.610 kb on - strand at 659.610 kb on - strand at 659.610 kb on - strand at 659.610 kb on - strand at 659.615 kb on - strand at 659.615 kb on - strand at 659.615 kb on - strand at 659.750 kb on - strand at 659.751 kb on + strand at 659.751 kb on + strand at 659.752 kb on - strand at 659.765 kb on + strand at 659.765 kb on + strand at 659.765 kb on + strand at 659.765 kb on + strand at 659.766 kb on - strand at 659.766 kb on - strand at 659.766 kb on - strand at 659.766 kb on - strand at 659.895 kb on + strand at 659.895 kb on + strand at 659.895 kb on + strand at 659.896 kb on - strand at 659.896 kb on - strand at 659.906 kb on - strand at 659.912 kb on + strand at 659.912 kb on + strand at 659.912 kb on + strand at 659.913 kb on - strand at 659.913 kb on - strand at 659.913 kb on - strand at 659.914 kb on + strand at 659.914 kb on + strand at 659.914 kb on + strand at 659.914 kb on + strand at 659.914 kb on + strand at 659.914 kb on + strand at 659.914 kb on + strand at 659.915 kb on - strand at 659.915 kb on - strand at 659.915 kb on - strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.919 kb on - strand at 659.919 kb on - strand at 659.919 kb on - strand at 659.919 kb on - strand at 659.919 kb on - strand at 659.919 kb on - strand at 659.919 kb on - strand at 659.919 kb on - strand at 659.919 kb on - strand at 659.936 kb on + strand at 659.936 kb on + strand at 659.936 kb on + strand at 660.158 kb on - strand at 660.158 kb on - strand at 660.158 kb on - strand at 660.158 kb on - strand at 660.175 kb on + strand at 660.175 kb on + strand at 660.176 kb on - strand at 660.176 kb on - strand at 660.176 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 657,167 + -0.3 657,167 + +2.2 657,167 + -0.7 657,167 + +0.4 657,168 - +0.4 657,168 - +0.6 657,278 + -1.3 657,278 + +1.6 657,278 + -0.9 657,279 - -1.6 657,279 - +0.0 657,279 - -0.4 657,351 + -0.4 657,351 + -0.7 657,351 + -1.7 657,351 + -1.1 657,352 - -0.6 657,352 - +0.4 657,352 - -0.0 657,352 - +0.7 657,352 - +0.4 657,352 - -0.7 657,352 - +0.4 657,352 - +1.6 657,352 - +2.0 657,352 - -0.3 657,352 - +0.2 657,493 - +1.7 657,602 - Psyr_0578 0.14 -0.3 657,774 + Psyr_0578 0.23 -0.4 657,774 + Psyr_0578 0.23 -1.5 657,774 + Psyr_0578 0.23 +0.2 657,775 - Psyr_0578 0.23 -0.4 657,775 - Psyr_0578 0.23 +0.0 657,775 - Psyr_0578 0.23 -1.8 657,775 - Psyr_0578 0.23 -0.4 657,775 - Psyr_0578 0.23 -0.6 657,775 - Psyr_0578 0.23 +1.6 657,775 - Psyr_0578 0.23 -0.6 657,775 - Psyr_0578 0.23 +0.0 657,775 - Psyr_0578 0.23 -1.0 657,775 - Psyr_0578 0.23 +1.2 657,775 - Psyr_0578 0.23 -1.4 657,788 + Psyr_0578 0.23 -0.2 657,814 + Psyr_0578 0.25 -0.4 657,815 - Psyr_0578 0.25 -0.3 657,821 + Psyr_0578 0.25 -0.2 657,822 - Psyr_0578 0.25 -1.2 657,822 - Psyr_0578 0.25 -1.6 657,822 - Psyr_0578 0.25 -0.3 657,822 - Psyr_0578 0.25 -0.2 657,822 - Psyr_0578 0.25 -1.1 657,926 + Psyr_0578 0.30 +0.6 657,926 + Psyr_0578 0.30 +0.2 657,926 + Psyr_0578 0.30 -0.4 657,927 - Psyr_0578 0.30 +0.6 657,927 - Psyr_0578 0.30 +0.2 657,927 - Psyr_0578 0.30 +1.7 657,927 - Psyr_0578 0.30 -0.2 657,927 - Psyr_0578 0.30 -1.9 657,927 - Psyr_0578 0.30 -2.8 657,927 - Psyr_0578 0.30 +1.1 657,927 - Psyr_0578 0.30 -1.2 657,929 + Psyr_0578 0.31 -1.0 657,930 - Psyr_0578 0.31 +0.6 657,930 - Psyr_0578 0.31 +0.1 657,930 - Psyr_0578 0.31 +0.3 657,930 - Psyr_0578 0.31 -1.3 657,987 + Psyr_0578 0.34 -0.1 657,988 - Psyr_0578 0.34 +0.3 658,245 + Psyr_0578 0.47 +0.4 658,245 + Psyr_0578 0.47 +0.6 658,246 - Psyr_0578 0.47 -0.6 658,255 - Psyr_0578 0.47 +0.3 658,387 + Psyr_0578 0.54 +0.2 658,387 + Psyr_0578 0.54 -0.9 658,388 - Psyr_0578 0.54 -0.2 658,388 - Psyr_0578 0.54 -1.8 658,388 - Psyr_0578 0.54 +0.0 658,388 - Psyr_0578 0.54 -0.7 658,388 - Psyr_0578 0.54 -1.4 658,388 - Psyr_0578 0.54 +0.5 658,429 + Psyr_0578 0.56 -0.6 658,430 - Psyr_0578 0.56 +3.0 658,660 + Psyr_0578 0.68 +0.4 658,661 - Psyr_0578 0.68 -0.3 658,661 - Psyr_0578 0.68 -1.7 658,786 + Psyr_0578 0.75 -0.8 658,825 + Psyr_0578 0.77 -0.6 658,826 - Psyr_0578 0.77 +0.9 658,924 + Psyr_0578 0.82 +0.1 658,924 + Psyr_0578 0.82 +0.0 659,019 + Psyr_0578 0.87 +0.2 659,020 - Psyr_0578 0.87 +1.0 659,482 - +0.8 659,502 + +0.2 659,502 + +0.4 659,504 + +0.3 659,505 - -0.3 659,505 - +1.6 659,525 + -0.7 659,543 + +0.1 659,543 + +0.5 659,543 + -0.7 659,544 - +0.4 659,547 - +0.4 659,610 - -1.0 659,610 - +0.1 659,610 - -0.5 659,610 - +1.0 659,615 - +0.0 659,615 - -0.2 659,615 - +0.6 659,750 - +1.0 659,751 + +0.0 659,751 + +0.5 659,752 - -1.2 659,765 + +0.0 659,765 + -0.4 659,765 + +0.5 659,765 + +0.1 659,766 - +0.2 659,766 - +1.4 659,766 - +0.0 659,766 - +2.0 659,895 + +0.1 659,895 + -0.8 659,895 + -0.7 659,896 - +2.0 659,896 - -0.6 659,906 - +1.0 659,912 + -0.3 659,912 + -1.7 659,912 + -1.0 659,913 - -0.7 659,913 - +0.8 659,913 - -2.0 659,914 + +1.0 659,914 + -1.6 659,914 + +0.9 659,914 + +0.9 659,914 + -0.9 659,914 + -0.1 659,914 + -0.3 659,915 - -0.6 659,915 - -0.2 659,915 - -1.6 659,916 + -0.5 659,916 + +0.7 659,916 + +1.3 659,916 + +0.2 659,916 + +1.4 659,916 + +0.0 659,916 + +1.7 659,916 + -1.5 659,916 + -0.5 659,916 + -1.2 659,916 + -0.2 659,916 + +0.5 659,916 + -0.4 659,916 + +0.1 659,916 + -0.1 659,916 + +0.3 659,916 + -0.9 659,916 + +1.4 659,916 + -2.4 659,916 + -0.4 659,916 + -0.7 659,916 + -0.1 659,917 - -1.6 659,917 - -0.6 659,917 - +0.0 659,917 - +0.6 659,917 - +0.0 659,917 - -1.0 659,917 - +0.4 659,917 - +1.0 659,917 - +0.8 659,917 - -0.7 659,917 - -1.0 659,917 - +0.0 659,917 - -1.0 659,917 - -1.0 659,917 - +1.0 659,917 - +0.0 659,917 - -0.7 659,918 + +0.7 659,918 + +1.0 659,918 + -0.6 659,918 + +0.0 659,918 + +0.4 659,918 + -0.5 659,918 + -1.9 659,918 + -1.4 659,918 + +1.2 659,918 + +0.8 659,918 + -0.4 659,918 + -0.6 659,918 + -0.3 659,918 + -0.3 659,918 + -0.6 659,918 + -0.6 659,918 + -1.1 659,919 - -0.3 659,919 - -1.0 659,919 - +0.5 659,919 - -0.4 659,919 - +0.3 659,919 - -0.6 659,919 - -1.3 659,919 - -1.8 659,919 - +2.0 659,936 + -0.6 659,936 + -0.8 659,936 + -1.1 660,158 - -1.2 660,158 - -1.1 660,158 - -1.7 660,158 - -0.7 660,175 + -1.0 660,175 + +1.0 660,176 - +1.4 660,176 - -1.4 660,176 - -1.0
Or see this region's nucleotide sequence