Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0540 and Psyr_0541 are separated by 48 nucleotides Psyr_0541 and Psyr_0542 are separated by 204 nucleotides
Psyr_0540: Psyr_0540 - oxidoreductase, FAD-binding protein, at 610,174 to 611,349
_0540
Psyr_0541: Psyr_0541 - Small multidrug resistance protein, at 611,398 to 611,730
_0541
Psyr_0542: Psyr_0542 - Three-deoxy-D-manno-octulosonic-acid transferase, N-terminal, at 611,935 to 613,080
_0542
Position (kb) 611
612 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 611.338 kb on - strand at 611.382 kb on - strand at 611.382 kb on - strand at 611.382 kb on - strand at 611.382 kb on - strand at 611.382 kb on - strand at 611.382 kb on - strand at 611.383 kb on + strand at 611.383 kb on + strand at 611.384 kb on - strand at 611.384 kb on - strand at 611.384 kb on - strand at 611.384 kb on - strand at 611.495 kb on + strand, within Psyr_0541 at 611.552 kb on + strand, within Psyr_0541 at 611.552 kb on + strand, within Psyr_0541 at 611.552 kb on + strand, within Psyr_0541 at 611.552 kb on + strand, within Psyr_0541 at 611.553 kb on - strand, within Psyr_0541 at 611.717 kb on + strand at 611.717 kb on + strand at 611.717 kb on + strand at 611.718 kb on - strand at 611.721 kb on - strand at 611.721 kb on - strand at 611.721 kb on - strand at 611.762 kb on + strand at 611.762 kb on + strand at 611.762 kb on + strand at 611.805 kb on + strand at 611.815 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 611,338 - -1.2 611,382 - -2.6 611,382 - -1.6 611,382 - -2.2 611,382 - -1.3 611,382 - -3.2 611,382 - -1.0 611,383 + -0.4 611,383 + -2.4 611,384 - -2.2 611,384 - -1.9 611,384 - -1.8 611,384 - -0.4 611,495 + Psyr_0541 0.29 -0.6 611,552 + Psyr_0541 0.46 -1.6 611,552 + Psyr_0541 0.46 -3.2 611,552 + Psyr_0541 0.46 -3.2 611,552 + Psyr_0541 0.46 -1.6 611,553 - Psyr_0541 0.47 -3.2 611,717 + -3.6 611,717 + -0.8 611,717 + -2.0 611,718 - -4.6 611,721 - -1.3 611,721 - -2.4 611,721 - -2.0 611,762 + -2.8 611,762 + -2.3 611,762 + -2.3 611,805 + -1.7 611,815 + -1.6
Or see this region's nucleotide sequence