Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_0479

Experiment: KB with Berberine 250 ug/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_0478 and Psyr_0479 are separated by 94 nucleotidesPsyr_0479 and Psyr_0480 are separated by 250 nucleotidesPsyr_0480 and Psyr_0481 are separated by 282 nucleotides Psyr_0478: Psyr_0478 - pilus retraction ATPase PilT, at 516,463 to 517,497 _0478 Psyr_0479: Psyr_0479 - NLP/P60, at 517,592 to 518,269 _0479 Psyr_0480: Psyr_0480 - TM2, at 518,520 to 518,924 _0480 Psyr_0481: Psyr_0481 - dihydroorotase, at 519,207 to 520,478 _0481 Position (kb) 517 518 519Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 516.616 kb on + strand, within Psyr_0478at 516.616 kb on + strand, within Psyr_0478at 516.616 kb on + strand, within Psyr_0478at 516.616 kb on + strand, within Psyr_0478at 516.617 kb on - strand, within Psyr_0478at 516.617 kb on - strand, within Psyr_0478at 516.617 kb on - strand, within Psyr_0478at 516.617 kb on - strand, within Psyr_0478at 516.817 kb on - strand, within Psyr_0478at 516.898 kb on + strand, within Psyr_0478at 516.899 kb on - strand, within Psyr_0478at 516.899 kb on - strand, within Psyr_0478at 516.906 kb on + strand, within Psyr_0478at 516.912 kb on + strand, within Psyr_0478at 516.912 kb on + strand, within Psyr_0478at 516.912 kb on + strand, within Psyr_0478at 516.912 kb on + strand, within Psyr_0478at 516.913 kb on - strand, within Psyr_0478at 516.924 kb on + strand, within Psyr_0478at 516.924 kb on + strand, within Psyr_0478at 516.924 kb on + strand, within Psyr_0478at 516.925 kb on - strand, within Psyr_0478at 516.925 kb on - strand, within Psyr_0478at 516.925 kb on - strand, within Psyr_0478at 516.925 kb on - strand, within Psyr_0478at 517.133 kb on + strand, within Psyr_0478at 517.366 kb on + strand, within Psyr_0478at 517.366 kb on + strand, within Psyr_0478at 517.366 kb on + strand, within Psyr_0478at 517.366 kb on + strand, within Psyr_0478at 517.366 kb on + strand, within Psyr_0478at 517.367 kb on - strand, within Psyr_0478at 517.367 kb on - strand, within Psyr_0478at 517.367 kb on - strand, within Psyr_0478at 517.367 kb on - strand, within Psyr_0478at 517.367 kb on - strand, within Psyr_0478at 517.367 kb on - strand, within Psyr_0478at 517.488 kb on + strandat 517.654 kb on - strandat 517.743 kb on + strand, within Psyr_0479at 517.743 kb on + strand, within Psyr_0479at 517.744 kb on - strand, within Psyr_0479at 517.896 kb on + strand, within Psyr_0479at 517.896 kb on + strand, within Psyr_0479at 517.896 kb on + strand, within Psyr_0479at 517.896 kb on + strand, within Psyr_0479at 517.896 kb on + strand, within Psyr_0479at 517.896 kb on + strand, within Psyr_0479at 517.896 kb on + strand, within Psyr_0479at 517.896 kb on + strand, within Psyr_0479at 517.897 kb on - strand, within Psyr_0479at 517.897 kb on - strand, within Psyr_0479at 517.897 kb on - strand, within Psyr_0479at 517.986 kb on - strand, within Psyr_0479at 518.167 kb on - strand, within Psyr_0479at 518.167 kb on - strand, within Psyr_0479at 518.227 kb on + strandat 518.227 kb on + strandat 518.227 kb on + strandat 518.227 kb on + strandat 518.227 kb on + strandat 518.227 kb on + strandat 518.227 kb on + strandat 518.227 kb on + strandat 518.227 kb on + strandat 518.227 kb on + strandat 518.227 kb on + strandat 518.228 kb on - strandat 518.228 kb on - strandat 518.228 kb on - strandat 518.228 kb on - strandat 518.228 kb on - strandat 518.228 kb on - strandat 518.228 kb on - strandat 518.228 kb on - strandat 518.228 kb on - strandat 518.231 kb on + strandat 518.231 kb on + strandat 518.231 kb on + strandat 518.231 kb on + strandat 518.231 kb on + strandat 518.303 kb on + strandat 518.303 kb on + strandat 518.303 kb on + strandat 518.303 kb on + strandat 518.303 kb on + strandat 518.303 kb on + strandat 518.303 kb on + strandat 518.304 kb on - strandat 518.304 kb on - strandat 518.306 kb on - strandat 518.449 kb on + strandat 518.449 kb on + strandat 518.449 kb on + strandat 518.460 kb on - strandat 518.485 kb on - strandat 518.604 kb on + strand, within Psyr_0480at 518.605 kb on - strand, within Psyr_0480at 518.605 kb on - strand, within Psyr_0480at 518.605 kb on - strand, within Psyr_0480at 518.605 kb on - strand, within Psyr_0480at 518.605 kb on - strand, within Psyr_0480at 518.605 kb on - strand, within Psyr_0480at 518.605 kb on - strand, within Psyr_0480at 518.742 kb on + strand, within Psyr_0480at 518.799 kb on + strand, within Psyr_0480at 518.799 kb on + strand, within Psyr_0480at 518.799 kb on + strand, within Psyr_0480at 518.800 kb on - strand, within Psyr_0480at 518.800 kb on - strand, within Psyr_0480at 518.800 kb on - strand, within Psyr_0480at 518.800 kb on - strand, within Psyr_0480at 518.800 kb on - strand, within Psyr_0480at 518.800 kb on - strand, within Psyr_0480at 518.800 kb on - strand, within Psyr_0480at 518.868 kb on + strand, within Psyr_0480at 518.869 kb on - strand, within Psyr_0480at 518.966 kb on + strandat 518.966 kb on + strandat 518.966 kb on + strandat 518.967 kb on - strandat 518.967 kb on - strandat 518.967 kb on - strandat 518.988 kb on - strandat 519.164 kb on + strandat 519.218 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml
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516,616 + Psyr_0478 0.15 -0.9
516,616 + Psyr_0478 0.15 -0.2
516,616 + Psyr_0478 0.15 -2.2
516,616 + Psyr_0478 0.15 +0.0
516,617 - Psyr_0478 0.15 -3.4
516,617 - Psyr_0478 0.15 +0.0
516,617 - Psyr_0478 0.15 -2.3
516,617 - Psyr_0478 0.15 -0.9
516,817 - Psyr_0478 0.34 +1.0
516,898 + Psyr_0478 0.42 -1.3
516,899 - Psyr_0478 0.42 +0.4
516,899 - Psyr_0478 0.42 +0.7
516,906 + Psyr_0478 0.43 -0.9
516,912 + Psyr_0478 0.43 -0.5
516,912 + Psyr_0478 0.43 +0.0
516,912 + Psyr_0478 0.43 +0.2
516,912 + Psyr_0478 0.43 -0.1
516,913 - Psyr_0478 0.43 +0.1
516,924 + Psyr_0478 0.45 -1.9
516,924 + Psyr_0478 0.45 +0.4
516,924 + Psyr_0478 0.45 -1.0
516,925 - Psyr_0478 0.45 +0.7
516,925 - Psyr_0478 0.45 -1.2
516,925 - Psyr_0478 0.45 +1.4
516,925 - Psyr_0478 0.45 +0.8
517,133 + Psyr_0478 0.65 -0.9
517,366 + Psyr_0478 0.87 -1.2
517,366 + Psyr_0478 0.87 -1.3
517,366 + Psyr_0478 0.87 -2.0
517,366 + Psyr_0478 0.87 -3.4
517,366 + Psyr_0478 0.87 -1.0
517,367 - Psyr_0478 0.87 +0.2
517,367 - Psyr_0478 0.87 -1.4
517,367 - Psyr_0478 0.87 -0.1
517,367 - Psyr_0478 0.87 -0.3
517,367 - Psyr_0478 0.87 +0.8
517,367 - Psyr_0478 0.87 -0.1
517,488 + +0.7
517,654 - -1.0
517,743 + Psyr_0479 0.22 -2.8
517,743 + Psyr_0479 0.22 -3.0
517,744 - Psyr_0479 0.22 +0.3
517,896 + Psyr_0479 0.45 -2.3
517,896 + Psyr_0479 0.45 -2.9
517,896 + Psyr_0479 0.45 -1.6
517,896 + Psyr_0479 0.45 -2.3
517,896 + Psyr_0479 0.45 -0.8
517,896 + Psyr_0479 0.45 -2.0
517,896 + Psyr_0479 0.45 -1.7
517,896 + Psyr_0479 0.45 -0.6
517,897 - Psyr_0479 0.45 +0.9
517,897 - Psyr_0479 0.45 -0.3
517,897 - Psyr_0479 0.45 +0.2
517,986 - Psyr_0479 0.58 +0.8
518,167 - Psyr_0479 0.85 +1.3
518,167 - Psyr_0479 0.85 +0.4
518,227 + -1.9
518,227 + -3.6
518,227 + -2.6
518,227 + -2.3
518,227 + -2.5
518,227 + -3.9
518,227 + -5.3
518,227 + -1.6
518,227 + -1.0
518,227 + -0.6
518,227 + -2.5
518,228 - -0.9
518,228 - +0.0
518,228 - -0.2
518,228 - +1.5
518,228 - -0.9
518,228 - +0.6
518,228 - +0.2
518,228 - +1.4
518,228 - -1.0
518,231 + -3.1
518,231 + -3.7
518,231 + -3.0
518,231 + -0.6
518,231 + -0.8
518,303 + -2.3
518,303 + -3.2
518,303 + -3.2
518,303 + -4.2
518,303 + -0.7
518,303 + -2.2
518,303 + -3.2
518,304 - -0.3
518,304 - +0.1
518,306 - -1.3
518,449 + -1.8
518,449 + -4.9
518,449 + -1.3
518,460 - +0.6
518,485 - +1.6
518,604 + Psyr_0480 0.21 +0.2
518,605 - Psyr_0480 0.21 +0.8
518,605 - Psyr_0480 0.21 +0.6
518,605 - Psyr_0480 0.21 +0.1
518,605 - Psyr_0480 0.21 +0.7
518,605 - Psyr_0480 0.21 +1.3
518,605 - Psyr_0480 0.21 +0.2
518,605 - Psyr_0480 0.21 +0.7
518,742 + Psyr_0480 0.55 +0.5
518,799 + Psyr_0480 0.69 -0.5
518,799 + Psyr_0480 0.69 +0.6
518,799 + Psyr_0480 0.69 +1.1
518,800 - Psyr_0480 0.69 +2.3
518,800 - Psyr_0480 0.69 +1.0
518,800 - Psyr_0480 0.69 -0.3
518,800 - Psyr_0480 0.69 -1.6
518,800 - Psyr_0480 0.69 -0.7
518,800 - Psyr_0480 0.69 +1.0
518,800 - Psyr_0480 0.69 +1.3
518,868 + Psyr_0480 0.86 +0.9
518,869 - Psyr_0480 0.86 +2.0
518,966 + +0.7
518,966 + +2.4
518,966 + +0.0
518,967 - +0.1
518,967 - +0.6
518,967 - +0.4
518,988 - +2.8
519,164 + +1.3
519,218 - +0.0

Or see this region's nucleotide sequence