Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0131 and Psyr_0132 are separated by 100 nucleotides Psyr_0132 and Psyr_0133 are separated by 83 nucleotides Psyr_0133 and Psyr_0134 are separated by 851 nucleotides
Psyr_0131: Psyr_0131 - HAD-superfamily hydrolase, subfamily IA, variant 3:HAD-superfamily hydrolase, subfamily IA, variant 1, at 139,587 to 140,261
_0131
Psyr_0132: Psyr_0132 - conserved hypothetical protein, at 140,362 to 140,610
_0132
Psyr_0133: Psyr_0133 - transcriptional regulator, AsnC family, at 140,694 to 141,164
_0133
Psyr_0134: Psyr_0134 - Aminotransferase, class V, at 142,016 to 143,203
_0134
Position (kb)
140
141
142 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 139.710 kb on - strand, within Psyr_0131 at 139.710 kb on - strand, within Psyr_0131 at 139.827 kb on + strand at 139.828 kb on - strand, within Psyr_0131 at 140.014 kb on - strand, within Psyr_0131 at 140.014 kb on - strand, within Psyr_0131 at 140.059 kb on + strand, within Psyr_0131 at 140.059 kb on + strand, within Psyr_0131 at 140.059 kb on + strand, within Psyr_0131 at 140.059 kb on + strand, within Psyr_0131 at 140.059 kb on + strand, within Psyr_0131 at 140.059 kb on + strand, within Psyr_0131 at 140.060 kb on - strand, within Psyr_0131 at 140.205 kb on + strand at 140.205 kb on + strand at 140.205 kb on + strand at 140.205 kb on + strand at 140.206 kb on - strand at 140.206 kb on - strand at 140.206 kb on - strand at 140.206 kb on - strand at 140.206 kb on - strand at 140.206 kb on - strand at 140.206 kb on - strand at 140.206 kb on - strand at 140.206 kb on - strand at 140.206 kb on - strand at 140.265 kb on + strand at 140.305 kb on + strand at 140.305 kb on + strand at 140.306 kb on - strand at 140.306 kb on - strand at 140.306 kb on - strand at 140.306 kb on - strand at 140.306 kb on - strand at 140.306 kb on - strand at 140.355 kb on + strand at 140.355 kb on + strand at 140.355 kb on + strand at 140.355 kb on + strand at 140.355 kb on + strand at 140.356 kb on - strand at 140.356 kb on - strand at 140.356 kb on - strand at 140.435 kb on + strand, within Psyr_0132 at 140.435 kb on + strand, within Psyr_0132 at 140.436 kb on - strand, within Psyr_0132 at 140.436 kb on - strand, within Psyr_0132 at 140.436 kb on - strand, within Psyr_0132 at 140.436 kb on - strand, within Psyr_0132 at 140.436 kb on - strand, within Psyr_0132 at 140.540 kb on + strand, within Psyr_0132 at 140.593 kb on + strand at 140.593 kb on + strand at 140.593 kb on + strand at 140.593 kb on + strand at 140.593 kb on + strand at 140.593 kb on + strand at 140.593 kb on + strand at 140.593 kb on + strand at 140.593 kb on + strand at 140.593 kb on + strand at 140.593 kb on + strand at 140.594 kb on - strand at 140.594 kb on - strand at 140.594 kb on - strand at 140.594 kb on - strand at 140.594 kb on - strand at 140.713 kb on + strand at 140.713 kb on + strand at 140.714 kb on - strand at 140.714 kb on - strand at 140.806 kb on + strand, within Psyr_0133 at 140.807 kb on - strand, within Psyr_0133 at 140.807 kb on - strand, within Psyr_0133 at 140.854 kb on + strand, within Psyr_0133 at 140.854 kb on + strand, within Psyr_0133 at 140.854 kb on + strand, within Psyr_0133 at 140.854 kb on + strand, within Psyr_0133 at 140.855 kb on - strand, within Psyr_0133 at 140.855 kb on - strand, within Psyr_0133 at 140.855 kb on - strand, within Psyr_0133 at 140.855 kb on - strand, within Psyr_0133 at 140.855 kb on - strand, within Psyr_0133 at 140.855 kb on - strand, within Psyr_0133 at 140.855 kb on - strand, within Psyr_0133 at 140.907 kb on + strand, within Psyr_0133 at 140.908 kb on - strand, within Psyr_0133 at 141.071 kb on + strand, within Psyr_0133 at 141.190 kb on + strand at 141.190 kb on + strand at 141.191 kb on - strand at 141.191 kb on - strand at 141.191 kb on - strand at 141.191 kb on - strand at 141.191 kb on - strand at 141.191 kb on - strand at 141.191 kb on - strand at 141.191 kb on - strand at 141.233 kb on - strand at 141.233 kb on - strand at 141.233 kb on - strand at 141.233 kb on - strand at 141.233 kb on - strand at 141.561 kb on - strand at 141.561 kb on - strand at 141.813 kb on + strand at 141.813 kb on + strand at 141.813 kb on + strand at 141.813 kb on + strand at 141.814 kb on - strand at 141.814 kb on - strand at 141.814 kb on - strand at 141.814 kb on - strand at 141.826 kb on + strand at 141.881 kb on - strand at 141.948 kb on + strand at 141.949 kb on - strand at 141.952 kb on + strand at 141.952 kb on + strand at 141.953 kb on - strand at 141.980 kb on + strand at 141.981 kb on - strand at 141.981 kb on - strand at 142.033 kb on - strand at 142.033 kb on - strand at 142.067 kb on + strand at 142.067 kb on + strand at 142.076 kb on + strand at 142.077 kb on - strand at 142.077 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 139,710 - Psyr_0131 0.18 -0.4 139,710 - Psyr_0131 0.18 -1.9 139,827 + -0.7 139,828 - Psyr_0131 0.36 +0.3 140,014 - Psyr_0131 0.63 +0.0 140,014 - Psyr_0131 0.63 -0.5 140,059 + Psyr_0131 0.70 +0.2 140,059 + Psyr_0131 0.70 +0.5 140,059 + Psyr_0131 0.70 +0.1 140,059 + Psyr_0131 0.70 -1.1 140,059 + Psyr_0131 0.70 +1.1 140,059 + Psyr_0131 0.70 -0.2 140,060 - Psyr_0131 0.70 -1.2 140,205 + +0.7 140,205 + -2.4 140,205 + -1.2 140,205 + -1.5 140,206 - +0.1 140,206 - +0.3 140,206 - -0.5 140,206 - +1.2 140,206 - -0.5 140,206 - +0.9 140,206 - -0.2 140,206 - +0.2 140,206 - -0.3 140,206 - +0.0 140,265 + +0.4 140,305 + +0.5 140,305 + -1.1 140,306 - +0.8 140,306 - +0.4 140,306 - -2.2 140,306 - +1.6 140,306 - -0.9 140,306 - -0.2 140,355 + +0.7 140,355 + +0.5 140,355 + +0.9 140,355 + +0.2 140,355 + -0.8 140,356 - +0.5 140,356 - +0.1 140,356 - +0.7 140,435 + Psyr_0132 0.29 -0.6 140,435 + Psyr_0132 0.29 -0.6 140,436 - Psyr_0132 0.30 -0.1 140,436 - Psyr_0132 0.30 +1.9 140,436 - Psyr_0132 0.30 -0.7 140,436 - Psyr_0132 0.30 -0.9 140,436 - Psyr_0132 0.30 +0.4 140,540 + Psyr_0132 0.71 +0.3 140,593 + +1.0 140,593 + +0.7 140,593 + +0.3 140,593 + +0.9 140,593 + +0.1 140,593 + -0.0 140,593 + -0.1 140,593 + -0.2 140,593 + -0.1 140,593 + +0.5 140,593 + +1.6 140,594 - +0.0 140,594 - +0.1 140,594 - -0.4 140,594 - +1.1 140,594 - -0.2 140,713 + +0.5 140,713 + -0.2 140,714 - -0.9 140,714 - +0.9 140,806 + Psyr_0133 0.24 -0.0 140,807 - Psyr_0133 0.24 +0.2 140,807 - Psyr_0133 0.24 -0.7 140,854 + Psyr_0133 0.34 -0.1 140,854 + Psyr_0133 0.34 -0.4 140,854 + Psyr_0133 0.34 -0.1 140,854 + Psyr_0133 0.34 +0.2 140,855 - Psyr_0133 0.34 -0.1 140,855 - Psyr_0133 0.34 -1.0 140,855 - Psyr_0133 0.34 -1.1 140,855 - Psyr_0133 0.34 -0.8 140,855 - Psyr_0133 0.34 -0.7 140,855 - Psyr_0133 0.34 -2.2 140,855 - Psyr_0133 0.34 -0.4 140,907 + Psyr_0133 0.45 -0.7 140,908 - Psyr_0133 0.45 -1.6 141,071 + Psyr_0133 0.80 -0.1 141,190 + +0.3 141,190 + +0.5 141,191 - -0.1 141,191 - +0.9 141,191 - +0.3 141,191 - +1.2 141,191 - -0.1 141,191 - -0.4 141,191 - +0.9 141,191 - -0.1 141,233 - -0.8 141,233 - -0.0 141,233 - +0.9 141,233 - -1.2 141,233 - +1.3 141,561 - -0.0 141,561 - +0.3 141,813 + -0.0 141,813 + -0.9 141,813 + +1.3 141,813 + +0.1 141,814 - +1.7 141,814 - -1.0 141,814 - +0.5 141,814 - -0.4 141,826 + -0.5 141,881 - +0.9 141,948 + -0.1 141,949 - -1.3 141,952 + -0.2 141,952 + +1.0 141,953 - +0.5 141,980 + -0.5 141,981 - +1.3 141,981 - +0.4 142,033 - +0.9 142,033 - +0.0 142,067 + +0.7 142,067 + +0.8 142,076 + -0.4 142,077 - +0.0 142,077 - -0.7
Or see this region's nucleotide sequence