Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0099 and Psyr_0100 are separated by 893 nucleotides Psyr_0100 and Psyr_0101 are separated by 18 nucleotides Psyr_0101 and Psyr_0102 are separated by 418 nucleotides
Psyr_0099: Psyr_0099 - Conserved hypothetical protein, at 111,332 to 111,541
_0099
Psyr_0100: Psyr_0100 - conserved hypothetical protein, at 112,435 to 112,968
_0100
Psyr_0101: Psyr_0101 - Protein of unknown function DUF796, at 112,987 to 113,487
_0101
Psyr_0102: Psyr_0102 - hypothetical protein, at 113,906 to 114,502
_0102
Position (kb)
112
113 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 111.444 kb on + strand, within Psyr_0099 at 111.512 kb on - strand, within Psyr_0099 at 111.523 kb on + strand at 111.527 kb on + strand at 111.543 kb on + strand at 111.581 kb on + strand at 111.582 kb on - strand at 111.590 kb on - strand at 111.624 kb on - strand at 111.712 kb on - strand at 111.716 kb on + strand at 111.716 kb on + strand at 111.717 kb on - strand at 111.717 kb on - strand at 111.717 kb on - strand at 111.717 kb on - strand at 111.756 kb on - strand at 111.801 kb on + strand at 111.801 kb on + strand at 111.801 kb on + strand at 111.801 kb on + strand at 111.801 kb on + strand at 111.802 kb on - strand at 111.802 kb on - strand at 111.802 kb on - strand at 111.802 kb on - strand at 111.905 kb on - strand at 111.906 kb on + strand at 111.906 kb on + strand at 111.906 kb on + strand at 111.906 kb on + strand at 111.906 kb on + strand at 111.907 kb on - strand at 111.907 kb on - strand at 111.908 kb on + strand at 111.908 kb on + strand at 111.909 kb on - strand at 111.910 kb on + strand at 111.910 kb on + strand at 111.910 kb on + strand at 111.910 kb on + strand at 111.911 kb on - strand at 111.911 kb on - strand at 111.911 kb on - strand at 111.911 kb on - strand at 111.914 kb on + strand at 111.914 kb on + strand at 111.915 kb on - strand at 111.915 kb on - strand at 111.915 kb on - strand at 111.916 kb on + strand at 111.916 kb on + strand at 111.917 kb on - strand at 111.917 kb on - strand at 111.917 kb on - strand at 111.917 kb on - strand at 111.960 kb on - strand at 111.973 kb on + strand at 111.973 kb on + strand at 111.974 kb on - strand at 111.997 kb on - strand at 111.997 kb on - strand at 112.021 kb on - strand at 112.115 kb on + strand at 112.147 kb on - strand at 112.173 kb on + strand at 112.173 kb on + strand at 112.173 kb on + strand at 112.522 kb on + strand, within Psyr_0100 at 112.522 kb on + strand, within Psyr_0100 at 112.522 kb on + strand, within Psyr_0100 at 112.523 kb on - strand, within Psyr_0100 at 112.527 kb on - strand, within Psyr_0100 at 112.527 kb on - strand, within Psyr_0100 at 112.531 kb on - strand, within Psyr_0100 at 112.711 kb on + strand, within Psyr_0100 at 112.711 kb on + strand, within Psyr_0100 at 112.712 kb on - strand, within Psyr_0100 at 112.720 kb on + strand, within Psyr_0100 at 112.770 kb on + strand, within Psyr_0100 at 112.770 kb on + strand, within Psyr_0100 at 112.770 kb on + strand, within Psyr_0100 at 112.772 kb on + strand, within Psyr_0100 at 112.772 kb on + strand, within Psyr_0100 at 112.772 kb on + strand, within Psyr_0100 at 112.772 kb on + strand, within Psyr_0100 at 112.772 kb on + strand, within Psyr_0100 at 112.772 kb on + strand, within Psyr_0100 at 112.772 kb on + strand, within Psyr_0100 at 112.772 kb on + strand, within Psyr_0100 at 112.773 kb on - strand, within Psyr_0100 at 112.773 kb on - strand, within Psyr_0100 at 112.773 kb on - strand, within Psyr_0100 at 112.773 kb on - strand, within Psyr_0100 at 112.773 kb on - strand, within Psyr_0100 at 112.862 kb on + strand, within Psyr_0100 at 112.875 kb on + strand, within Psyr_0100 at 112.931 kb on - strand at 112.968 kb on + strand at 112.968 kb on + strand at 112.968 kb on + strand at 112.968 kb on + strand at 112.968 kb on + strand at 112.968 kb on + strand at 112.968 kb on + strand at 112.968 kb on + strand at 112.968 kb on + strand at 112.968 kb on + strand at 112.968 kb on + strand at 112.968 kb on + strand at 112.968 kb on + strand at 112.969 kb on - strand at 112.969 kb on - strand at 112.969 kb on - strand at 112.969 kb on - strand at 112.969 kb on - strand at 112.969 kb on - strand at 112.969 kb on - strand at 112.969 kb on - strand at 112.969 kb on - strand at 112.969 kb on - strand at 112.994 kb on + strand at 112.994 kb on + strand at 112.995 kb on - strand at 112.996 kb on + strand at 112.996 kb on + strand at 112.996 kb on + strand at 112.997 kb on - strand at 112.997 kb on - strand at 113.070 kb on + strand, within Psyr_0101 at 113.070 kb on + strand, within Psyr_0101 at 113.071 kb on - strand, within Psyr_0101 at 113.071 kb on - strand, within Psyr_0101 at 113.072 kb on + strand, within Psyr_0101 at 113.072 kb on + strand, within Psyr_0101 at 113.072 kb on + strand, within Psyr_0101 at 113.072 kb on + strand, within Psyr_0101 at 113.072 kb on + strand, within Psyr_0101 at 113.072 kb on + strand, within Psyr_0101 at 113.072 kb on + strand, within Psyr_0101 at 113.073 kb on - strand, within Psyr_0101 at 113.073 kb on - strand, within Psyr_0101 at 113.073 kb on - strand, within Psyr_0101 at 113.073 kb on - strand, within Psyr_0101 at 113.073 kb on - strand, within Psyr_0101 at 113.073 kb on - strand, within Psyr_0101 at 113.073 kb on - strand, within Psyr_0101 at 113.073 kb on - strand, within Psyr_0101 at 113.093 kb on + strand, within Psyr_0101 at 113.093 kb on + strand, within Psyr_0101 at 113.094 kb on - strand, within Psyr_0101 at 113.131 kb on + strand, within Psyr_0101 at 113.131 kb on + strand, within Psyr_0101 at 113.131 kb on + strand, within Psyr_0101 at 113.131 kb on + strand, within Psyr_0101 at 113.132 kb on - strand, within Psyr_0101 at 113.166 kb on + strand, within Psyr_0101 at 113.166 kb on + strand, within Psyr_0101 at 113.185 kb on + strand, within Psyr_0101 at 113.185 kb on + strand, within Psyr_0101 at 113.186 kb on - strand, within Psyr_0101 at 113.186 kb on - strand, within Psyr_0101 at 113.196 kb on - strand, within Psyr_0101 at 113.325 kb on + strand, within Psyr_0101 at 113.326 kb on - strand at 113.392 kb on + strand, within Psyr_0101 at 113.392 kb on + strand, within Psyr_0101 at 113.472 kb on - strand at 113.472 kb on - strand at 113.472 kb on - strand at 113.493 kb on + strand at 113.516 kb on - strand at 113.523 kb on - strand at 113.534 kb on + strand at 113.534 kb on + strand at 113.535 kb on - strand at 113.535 kb on - strand at 113.535 kb on - strand at 113.654 kb on - strand at 113.735 kb on + strand at 113.736 kb on - strand at 113.737 kb on + strand at 113.737 kb on + strand at 113.737 kb on + strand at 113.737 kb on + strand at 113.737 kb on + strand at 113.737 kb on + strand at 113.737 kb on + strand at 113.737 kb on + strand at 113.738 kb on - strand at 113.738 kb on - strand at 113.738 kb on - strand at 113.751 kb on + strand at 113.751 kb on + strand at 113.755 kb on + strand at 113.755 kb on + strand at 113.755 kb on + strand at 113.755 kb on + strand at 113.755 kb on + strand at 113.755 kb on + strand at 113.755 kb on + strand at 113.755 kb on + strand at 113.755 kb on + strand at 113.755 kb on + strand at 113.755 kb on + strand at 113.755 kb on + strand at 113.755 kb on + strand at 113.755 kb on + strand at 113.755 kb on + strand at 113.756 kb on - strand at 113.756 kb on - strand at 113.756 kb on - strand at 113.756 kb on - strand at 113.756 kb on - strand at 113.756 kb on - strand at 113.756 kb on - strand at 113.756 kb on - strand at 113.756 kb on - strand at 113.756 kb on - strand at 113.756 kb on - strand at 113.793 kb on + strand at 113.793 kb on + strand at 113.793 kb on + strand at 113.794 kb on - strand at 113.804 kb on - strand at 113.875 kb on + strand at 113.875 kb on + strand at 113.876 kb on - strand at 113.876 kb on - strand at 113.876 kb on - strand at 113.876 kb on - strand at 113.876 kb on - strand at 113.876 kb on - strand at 113.963 kb on + strand at 113.964 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 111,444 + Psyr_0099 0.53 -0.1 111,512 - Psyr_0099 0.86 +0.2 111,523 + -0.3 111,527 + +0.5 111,543 + +0.2 111,581 + +0.9 111,582 - +0.1 111,590 - +0.1 111,624 - -0.9 111,712 - +0.1 111,716 + -1.1 111,716 + +0.3 111,717 - -0.3 111,717 - -0.4 111,717 - -0.4 111,717 - +0.2 111,756 - +0.2 111,801 + -0.7 111,801 + +0.3 111,801 + +0.8 111,801 + -0.6 111,801 + +0.9 111,802 - -0.1 111,802 - +0.2 111,802 - -0.5 111,802 - -0.7 111,905 - -0.0 111,906 + -1.7 111,906 + +0.0 111,906 + -0.1 111,906 + +0.3 111,906 + +1.1 111,907 - +0.0 111,907 - -1.1 111,908 + +0.3 111,908 + -0.1 111,909 - +0.1 111,910 + -1.0 111,910 + -1.8 111,910 + +0.7 111,910 + -0.5 111,911 - +0.1 111,911 - +0.9 111,911 - -1.2 111,911 - +0.5 111,914 + +0.9 111,914 + +0.1 111,915 - -0.3 111,915 - -1.3 111,915 - -0.9 111,916 + +0.4 111,916 + +0.2 111,917 - -0.3 111,917 - -1.4 111,917 - +0.7 111,917 - -0.1 111,960 - +0.2 111,973 + +0.6 111,973 + -0.2 111,974 - +1.6 111,997 - -1.2 111,997 - +0.3 112,021 - -0.4 112,115 + +0.7 112,147 - +0.1 112,173 + +0.1 112,173 + +0.4 112,173 + -0.7 112,522 + Psyr_0100 0.16 -0.7 112,522 + Psyr_0100 0.16 -1.1 112,522 + Psyr_0100 0.16 +2.7 112,523 - Psyr_0100 0.16 -1.2 112,527 - Psyr_0100 0.17 -0.1 112,527 - Psyr_0100 0.17 +1.2 112,531 - Psyr_0100 0.18 -0.1 112,711 + Psyr_0100 0.52 -0.4 112,711 + Psyr_0100 0.52 -0.5 112,712 - Psyr_0100 0.52 +1.2 112,720 + Psyr_0100 0.53 +1.3 112,770 + Psyr_0100 0.63 +0.9 112,770 + Psyr_0100 0.63 +0.9 112,770 + Psyr_0100 0.63 +0.7 112,772 + Psyr_0100 0.63 -0.2 112,772 + Psyr_0100 0.63 -0.0 112,772 + Psyr_0100 0.63 +1.1 112,772 + Psyr_0100 0.63 +0.4 112,772 + Psyr_0100 0.63 +1.1 112,772 + Psyr_0100 0.63 +0.5 112,772 + Psyr_0100 0.63 +0.9 112,772 + Psyr_0100 0.63 +0.9 112,773 - Psyr_0100 0.63 +2.1 112,773 - Psyr_0100 0.63 -0.1 112,773 - Psyr_0100 0.63 +1.6 112,773 - Psyr_0100 0.63 -0.3 112,773 - Psyr_0100 0.63 -0.1 112,862 + Psyr_0100 0.80 -0.1 112,875 + Psyr_0100 0.82 +1.3 112,931 - -0.1 112,968 + +0.4 112,968 + +0.0 112,968 + -0.1 112,968 + -0.5 112,968 + +0.8 112,968 + +0.8 112,968 + -0.8 112,968 + -0.6 112,968 + -0.7 112,968 + -1.1 112,968 + +0.5 112,968 + -0.5 112,968 + +1.2 112,969 - +0.2 112,969 - -1.3 112,969 - -0.8 112,969 - -0.4 112,969 - +0.2 112,969 - +0.1 112,969 - -0.3 112,969 - -0.1 112,969 - -2.5 112,969 - -0.7 112,994 + -1.3 112,994 + -1.2 112,995 - -0.8 112,996 + +1.1 112,996 + -1.9 112,996 + -0.1 112,997 - +0.4 112,997 - -0.0 113,070 + Psyr_0101 0.17 -1.5 113,070 + Psyr_0101 0.17 +0.8 113,071 - Psyr_0101 0.17 -0.7 113,071 - Psyr_0101 0.17 +0.5 113,072 + Psyr_0101 0.17 +0.6 113,072 + Psyr_0101 0.17 -0.3 113,072 + Psyr_0101 0.17 +0.8 113,072 + Psyr_0101 0.17 -0.3 113,072 + Psyr_0101 0.17 -0.6 113,072 + Psyr_0101 0.17 +0.4 113,072 + Psyr_0101 0.17 -0.5 113,073 - Psyr_0101 0.17 +0.8 113,073 - Psyr_0101 0.17 -0.4 113,073 - Psyr_0101 0.17 -0.1 113,073 - Psyr_0101 0.17 +0.9 113,073 - Psyr_0101 0.17 +0.1 113,073 - Psyr_0101 0.17 +1.3 113,073 - Psyr_0101 0.17 -0.5 113,073 - Psyr_0101 0.17 -0.1 113,093 + Psyr_0101 0.21 -0.5 113,093 + Psyr_0101 0.21 -1.1 113,094 - Psyr_0101 0.21 +0.3 113,131 + Psyr_0101 0.29 +0.1 113,131 + Psyr_0101 0.29 -2.1 113,131 + Psyr_0101 0.29 +0.8 113,131 + Psyr_0101 0.29 -0.9 113,132 - Psyr_0101 0.29 -1.2 113,166 + Psyr_0101 0.36 +1.3 113,166 + Psyr_0101 0.36 +0.0 113,185 + Psyr_0101 0.40 +0.8 113,185 + Psyr_0101 0.40 -0.9 113,186 - Psyr_0101 0.40 -1.7 113,186 - Psyr_0101 0.40 +0.4 113,196 - Psyr_0101 0.42 +0.6 113,325 + Psyr_0101 0.67 -0.1 113,326 - -1.1 113,392 + Psyr_0101 0.81 +0.5 113,392 + Psyr_0101 0.81 -0.4 113,472 - +0.4 113,472 - +1.3 113,472 - -0.1 113,493 + -0.8 113,516 - -1.4 113,523 - +0.0 113,534 + +0.3 113,534 + -0.1 113,535 - +0.9 113,535 - +0.5 113,535 - -0.1 113,654 - +0.1 113,735 + +0.7 113,736 - +0.1 113,737 + +0.7 113,737 + -0.7 113,737 + +0.7 113,737 + +0.1 113,737 + -0.9 113,737 + +0.6 113,737 + +0.1 113,737 + -1.9 113,738 - +0.3 113,738 - -2.5 113,738 - +0.8 113,751 + -0.3 113,751 + +0.1 113,755 + -0.1 113,755 + -1.5 113,755 + -1.1 113,755 + -0.1 113,755 + +0.2 113,755 + +1.4 113,755 + +0.9 113,755 + -0.7 113,755 + +0.2 113,755 + +0.5 113,755 + -0.3 113,755 + +0.2 113,755 + -0.7 113,755 + -0.9 113,755 + -0.5 113,756 - -1.0 113,756 - +0.2 113,756 - -0.8 113,756 - -0.8 113,756 - -0.6 113,756 - -0.7 113,756 - +1.7 113,756 - -0.3 113,756 - -0.1 113,756 - +0.8 113,756 - -0.0 113,793 + -0.1 113,793 + -0.4 113,793 + -0.3 113,794 - +0.6 113,804 - +0.9 113,875 + +0.2 113,875 + +0.5 113,876 - -0.9 113,876 - +0.4 113,876 - -2.4 113,876 - -0.1 113,876 - +0.3 113,876 - -0.7 113,963 + -0.7 113,964 - +0.2
Or see this region's nucleotide sequence